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Technologies
Themes / Divisions

About

The Genomics Laboratory implements advanced genomics technologies, seeking the most effective ways to prepare biological samples for sequencing on our Illumina, MGI, PacBio and Oxford Nanopore platforms.

Members of the Bowden Laboratory are expert in:

* new and established sequencing technologies
* pathogen sequencing and interrogating host-pathogen interactions
* single-cell and spatial genomics and “multi-omics”
* highly multiplexed sequencing
* high-throughput functional genomics
* high-throughput assays using iPSC and cellular/organoid models for disease

Our team enables genomics-based discovery through collaboration. WEHI’s 2021-2025 Genomics Strategic plan provides for the expansion of the Institute’s genomics capability in enhanced service, collaboration and R&D. We are focused on institutional provision, extensive collaboration and selected external partnerships centred in Parkville.

Publications

Selected publications from Prof Rory Bowden

Brown DV, Swierczak A, You Y, You Y, Amann-Zalcenstein D, Hickey P, Hsu A, Ritchie ME, Alhamdoosh M, Field J, Bowden R. A dataset examining technical factors on fixed white blood cell single-cell RNA-seq. Data in Brief. 2024;57:10.1016/j.dib.2024.111096

Zeglinski K, Montellese C, Ritchie ME, Alhamdoosh M, Vonarburg C, Bowden R, Jordi M, Gouil Q, Aeschimann F, Hsu A. An optimized protocol for quality control of gene therapy vectors using nanopore direct RNA sequencing.Genome Research. 2024;:10.1101/gr.279405.124

Baldi A, Braat S, Hasan MI, Bennett C, Barrios M, Jones N, Abdul Azeez I, Wilcox S, Roy PK, Bhuiyan MSA, Ataide R, Clucas D, Larson LM, Hamadani J, Zimmermann M, Bowden R, Jex A, Biggs B-A, Pasricha S-R. Effects of iron supplements and iron-containing micronutrient powders on the gut microbiome in Bangladeshi infants: a randomized controlled trial. Nature Communications. 2024;15(1):10.1038/s41467-024-53013-x

Kadambari S, Harvala H, Nguyen D, Sadarangani M, Martin NG, Al-Rawahi GN, Sekirov I, Defres S, Solomon T, Golubchik T, Bowden R, Pollard AJ, Simmonds P. Characterising the molecular epidemiology of human parechovirus in young infants in the UK and Canada. Journal of Clinical Virology. 2024;174:10.1016/j.jcv.2024.105715

Mayne R, Secret S, Geoghegan C, Trebes A, Kean K, Reid K, Lin G-L, Ansari MA, de Cesare M, Bonsall D, Elliott I, Piazza P, Brown A, Bray J, Knight JC, Harvala H, Breuer J, Simmonds P, Bowden RJ, Golubchik T. Castanet: a pipeline for rapid analysis of targeted multi-pathogen genomic data. Bioinformatics. 2024;40(10):10.1093/bioinformatics/btae591

Baldi A, Braat S, Imrul Hasan M, Bennett C, Barrios M, Jones N, Moir-Meyer G, Abdul Azeez I, Wilcox S, Saiful Alam Bhuiyan M, Ataide R, Clucas D, Harrison LC, Arifeen SE, Bowden R, Biggs B-A, Jex A, Pasricha S-R. Community use of oral antibiotics transiently reprofiles the intestinal microbiome in young Bangladeshi children. Nature Communications. 2024;15(1):10.1038/s41467-024-51326-5

Chiou S, Al-Ani AH, Pan Y, Patel KM, Kong IY, Whitehead LW, Light A, Young SN, Barrios M, Sargeant C, Rajasekhar P, Zhu L, Hempel A, Lin A, Rickard JA, Hall C, Gangatirkar P, Yip RK, Cawthorne W, Jacobsen AV, Horne CR, Martin KR, Ioannidis LJ, Hansen DS, Day J, Wicks IP, Law C, Ritchie ME, Bowden R, Hildebrand JM, O’Reilly LA, Silke J, Giulino-Roth L, Tsui E, Rogers KL, Hawkins ED, Christensen B, Murphy JM, Samson AL. An immunohistochemical atlas of necroptotic pathway expression. EMBO Molecular Medicine. 2024;16(7):10.1038/s44321-024-00074-6

Lin G-L, Drysdale SB, Snape MD, O’Connor D, Brown A, MacIntyre-Cockett G, Mellado-Gomez E, de Cesare M, Ansari MA, Bonsall D, Bray JE, Jolley KA, Bowden R, Aerssens J, Bont L, Openshaw PJM, Martinon-Torres F, Nair H, Golubchik T, Pollard AJ. Targeted metagenomics reveals association between severity and pathogen co-detection in infants with respiratory syncytial virus. Nature Communications. 2024;15(1):10.1038/s41467-024-46648-3

Brown DV, Anttila CJA, Ling L, Grave P, Baldwin TM, Munnings R, Farchione AJ, Bryant VL, Dunstone A, Biben C, Taoudi S, Weber TS, Naik SH, Hadla A, Barker HE, Vandenberg CJ, Dall G, Scott CL, Moore Z, Whittle JR, Freytag S, Best SA, Papenfuss AT, Olechnowicz SWZ, MacRaild SE, Wilcox S, Hickey PF, Amann-Zalcenstein D, Bowden R. A risk-reward examination of sample multiplexing reagents for single cell RNA-Seq. Genomics. 2024;116(2):10.1016/j.ygeno.2024.110793

Christensen KT, Pierard F, Bonsall D, Bowden R, Barnes E, Florence E, Ansari MA, Nguyen D, de Cesare M, Nevens F, Robaeys G, Schrooten Y, Busschots D, Simmonds P, Vandamme A-M, Van Wijngaerden E, Dierckx T, Cuypers L, Van Laethem K. Phylogenetic Analysis of Hepatitis C Virus Infections in a Large Belgian Cohort Using Next-Generation Sequencing of Full-Length Genomes. Viruses. 2023;15(12):10.3390/v15122391

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