Our lab seeks to understand how the protein kinase family organises complex intracellular communication networks. We are particularly interested in acquiring a deep understanding at the molecular level of the catalysis-independent signalling functions of kinase/pseudokinase scaffold.

We employ a multidisciplinary approach including kinase biochemistry and biology, structural biology (X-ray crystallography and cryo-electron microscopy), assay development, high-throughput screening, proteomics, imaging and chemical biology to gain high-resolution insights into kinase/pseudokinase signaling proteins.


Selected publications from Prof Isabelle Lucet

Carli ALE, Hardy JM, Hoblos H, Ernst M, Lucet IS, Buchert M. Structure-Guided Prediction of the Functional Impact of DCLK1 Mutations on Tumorigenesis. Biomedicines. 2023 Mar 22;11(3):990. doi: 10.3390/biomedicines11030990.

Patel O, Surudoi M, Dai W, Hardy J, Roy MJ, Lucet IS. Production and purification of the PEAK pseudokinases for structural and functional studies. Methods Enzymol. 2022;667:1-35. doi: 10.1016/bs.mie.2022.03.022.

Patel O, Brammananth R, Dai W, Panjikar S, Coppel RL, Lucet IS, Crellin PK. Crystal structure of the putative cell-wall lipoglycan biosynthesis protein LmcA from Mycobacterium smegmatis. Acta Crystallography D Struct Biol. (2022) Apr 1;78(Pt 4):494-508.

Hou J, Nguyen EV, Surudoi M, Roy MJ, Patel O, Lucet IS, Ma X, Daly RJ. Distinct PEAK3 interactors and outputs expand the signaling potential of the PEAK pseudokinase family. Sci Signal. 2022 Feb 22;15(722):eabj3554.

Patel O, Roy MJ, Kropp A, Hardy JM, Dai W, Lucet IS. Structural basis for small molecule targeting of Doublecortin like kinase 1 with DCLK1-IN-1. Commun Biol 2021 4(1):1105. DOI: 10.1038/s42003-021-02631-y PMID: 34545159

Liang LY, Roy M, Horne CR, Sandow JJ, Surudoi M, Dagley LF, Young SN, Dite T, Babon JJ, Janes PW, Patel O, Murphy JM, Lucet IS. The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs. Biochem J. 2021 Sep 17;478(17):3351-3371. doi: 10.1042/BCJ20210572. PMID: 34431498

Patel O, Roy MJ, Murphy JM, Lucet IS. The PEAK family of pseudokinases, their role in cell signalling and cancer. FEBS J. 2020 Oct;287(19):4183-4197. doi: 10.1111/febs.15087. PMID: 31599110

Liang LY, Patel O, Janes PW, Murphy JM, Lucet IS. Eph receptor signalling: from catalytic to non-catalytic functions. Oncogene. 2019 Sep;38(39):6567-6584. doi: 10.1038/s41388-019-0931-2. PMID: 31406248

Roy MJ, Winkler S, Hughes SJ, Whitworth C, Galant M, Farnaby W, Rumpel K and Ciulli A. SPR-measured dissociation kinetics of PROTAC ternary complexes influence target degradation rate. ACS Chem Biol. 2019 Mar 15;14(3):361-368. PMID: 30721025.

Patel O, Griffin MDW, Panjikar S, Dai W, Ma X, Chan H, Zheng C, Kropp A, Murphy JM, Daly RJ, Lucet IS. Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association. Nature Communications, 2017 Oct 27;8(1):1157. doi: 10.1038/s41467-017-01279-9. PMID: 29079850

Patel O, Dai W, Mentzel M, Griffin MD, Serindoux J, Gay Y, Fischer S, Sterle S, Kropp A, Burns CJ, Ernst M, Buchert M, Lucet IS. Biochemical and Structural Insights into Doublecortin-like Kinase Domain 1. Structure. 2016 Sep 6;24(9):1550-61. doi: 10.1016/j.str.2016.07.008. PMID: 27545623

Lab research projects

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