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Centre for Dynamic Imaging

Zeiss LSM 980 with Airyscan 2

A next generation confocal for fast and gentle 4D imaging.

The Zeiss LSM 980 with Airyscan 2 confocal microscope at the Walter and Eliza Hall Institute is one of the first available in the world. The new Airyscan detector module with the Definite Focus.2 and Z-Piezo drive offers unrivalled flexibility in fast, high resolution live cell imaging in a familiar confocal setup.

The system is compatible with most sample holders and contains all necessary accessories for environmental control, for example, CO2, humidity and temperature control.

Zeiss LSM 980 microscope

Objectives

Light sources

  • Diode laser – 405 nm
  • Argon laser – 445 nm, 488 nm and 514 nm
  • Diode pumped solid state laser – 561 nm
  • HeNe Laser – 594 nm
  • HeNe laser – 639 nm
  • HAL 100 Illuminator
  • HXP-120 120 V

Detection source

  • 32 channel GaAsP array detection
  • 2 x MA-PMT
  • Airyscan 2 detector
  • Transmitted-PMT
Magnification10x20x40x63x63x
NA0.450.81.31.41.2
Immersion mediaAirAirOilOilWater
XY-resolution (µm) (@ 488 nm)0.6610.3720.2290.2120.248
Z-resolution (µm) (@ 488 nm)4.8191.5240.8760.7550.902
Working distance (mm)2.0000.5500.210.1400.28

Technological specifications (capabilities)

Available modalities

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions
  • Spectral linear unmixing
  • Online fingerprinting
  • FRAP
  • Airyscan 2 detection
  • Fast Airyscan

Unique features

The brand new Airyscan 2 detector enables the fastest point-scanning confocal system on the market. Operating at up to 10 fps with the resolution of standard confocal or better with high Signal-to-Noise.

Limitation

  • Photobleaching
Contact us

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.

Centre for Dynamic Imaging

Zeiss LSM 880 Fast Airyscan Confocal

A high speed, high resolution microscope for investigating dynamic processes in living cells.

The Zeiss LSM 880 with fast Airyscan confocal microscope is the essential confocal platform for live cell imaging. The unique combination of the Airyscan detector module with the Definite Focus.2 and Z-Piezo drive offers unrivalled flexibility in fast, high resolution live cell imaging.

The system is compatible with most sample holders and contains all necessary accessories for environmental control (for example, CO2, humidity, and temperature control).

Above: Magnified snapshot of a mammary gland during lactation. The yellow patterns are tiny muscles that contract and release, helping to squeeze milk into the mammary ducts. Purple flecks in and around the structures are immune cells on high alert for any threats in order to help keep the breast tissue healthy. Credit: Caleb Dawson
Above: A Zeiss LSM 880 microscope at WEHI

Hardware

Light sources

  • Diode laser – 405 nm
  • Argon laser – 458 nm, 488 nm and 514 nm
  • Diode pumped solid state laser – 561 nm
  • HeNe Laser – 594 nm
  • HeNe laser – 633 nm
  • HAL 100 Illuminator
  • HXP-120 120 V

Detection sources

  • 32 channel GaAsP array detection
  • 2 x MA-PMT
  • Airyscan detector
  • Transmitted-PMT
Magnification10x20x40x63x63x
NA0.450.81.31.41.2
Immersion mediaAirAirOilOilWater
XY-resolution (µm) (@ 488 nm)0.6610.3720.2290.2120.248
Z-resolution (µm) (@ 488 nm)4.8191.5240.8760.7550.902
Working distance (mm)2.0000.5500.210.1400.28

Technological specifications (capabilities)

Available modalities

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions
  • Spectral linear unmixing
  • Online fingerprinting
  • Single point FCS
  • FRAP
  • Airyscan detection
  • Fast Airyscan

Additional hardware

  • Z-Piezo drive
  • Definite Focus.2

Unique features

  • The Zeiss LSM 880 combines the fast Airyscan modality with the Z-Piezo drive system to offer fast live cell three-dimensional imaging.
  • The Definite Focus.2 module provides one of the most robust systems for live time-lapse imaging.

Limitation

  • Photobleaching

Experts

Professor Kelly Rogers
  • Head, Centre for Dynamic Imaging
Cindy Evelyn
  • Microscopist
Dr Lachlan Whitehead
  • Bioimage analyst
Dr Niall Geoghegan
  • Lattice light sheet specialist
Contact us

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.

Centre for Dynamic Imaging

Leica SP8 Resonant Scanning Confocal

The Leica SP8 is a high speed, high resolution microscope for investigating dynamic processes in living cells.

The Leica SP8 Resonant Scanning Confocal microscope is suitable for live cell imaging with subcellular resolution. The system contains a resonant scanning confocal mode allowing for fast imaging of dynamic cellular processes

The system is compatible with most sample holders and contains all necessary accessories for environmental control, for example, CO2, humidity and temperature.

Above: Blood vessels sprouting out of a piece of lab-grown bone can be live-imaged, allowing researchers to better understand how blood vessel growth is controlled. Credit: Zoe Grant
Above: Blood vessels converging at the centre of the retina. Credit: Sabrina Lewis and Leigh Coultas

Objectives

Light sources 

  • Diode laser – 405 nm
  • Argon laser – 458 nm, 488 nm and 514 nm
  • Diode pumped solid state laser – 561 nm
  • HeNe Laser – 594 nm
  • HeNe laser – 633 nm

Detection sources 

  • 3 x PMT
  • 2 x HyD detector
Magnification10x20x40x40x63x100x
NA0.40.751.31.11.41.4
Immersion mediaAirWater/GlycerolOilWaterOilOil
XY-resolution (µm) (@ 488 nm)0.7440.3960.2290.2701.2120.212
Z-resolution (µm) (@ 488 nm)6.0992.3110.8761.9740.7550.755
Working distance (mm)2.20.680.240.650.1400.28
Above: Researchers are studying the architecture and cellular composition of the structures that branch from the end of mammary ducts – the sites where breast cancer usually arises. Credit: Bianca Capaldo
Above: This image, captured on the Leica SP8, shows a section of tissue staining for different proteins that play essential roles during development. Credit: Zoe Grant

Technological specifications (capabilities)

Available modalities 

  • Z-stack
  • Time-series
  • Tile scan
  • Multi positions
  • FRAP

Additional hardware 

  • FRAP
Above: Fluorescent marking can be used to trace how a single rogue cell can give rise to the many cells in a tumour. While studying the development of breast cancer, researchers found these spectacular fluorescent hairs forming luminous roads. Imaged on a Leica SP8 confocal microscope and processed using Imaris software (Bitplane). Credit: Caleb Dawson
Above: Researchers study the genes involved in myogenesis (a process of muscle formation) to develop therapies for treating disorders such as muscular dystrophy. In this image myoblast cells are fluorescently stained with DAPI (yellow) to stain the nucleus and with Sidt2-FITC labelled antibody (pink) to stain the cytoplasm. Credit: Marilou Barrios

Unique features

  • Resonant scanner for fast imaging
  • FRAP module
Above: Researchers have used the Leica SP8 to study blood vessels endothelias cells in the small intestine, to better understand how blood vessels develop. Credit: Evelyn Trounson, Zoe Grant, Leigh Coultas
Above: In this image masses of immune cells (macrophages) are indicated in bright green. The lone blue ring is a cell nucleus, all that remains after a macrophage has detected and consumed a threat (bacteria or viruses). The adjacent red dot is an ASC speck, which indicates that the macrophage called for backup in its final moments. Credit: Dom De Nardo

Limitations

  • Photobleaching
  • SNR using resonant scanner is poor – requires averaging for low signal

Acknowledgement

This microscope was purchased with the generous support of the Walter and Eliza Hall Trust.

Above: Blood vessels at the edge of the retina. Credit: Sabrina Lewis and Leigh Coultas
Above: Migrating blood vessels (red) and blood cells (white) are observed in the study of how platelets affect brain bleeds/stroke. Credit: Alison Farley

Experts

Professor Kelly Rogers
  • Head, Centre for Dynamic Imaging
Cindy Evelyn
  • Microscopist
Dr Lachlan Whitehead
  • Bioimage analyst
Dr Niall Geoghegan
  • Lattice light sheet specialist
Contact us

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.