Zeiss LSM 880 Fast Airyscan Confocal

A high speed, high resolution microscope for investigating dynamic processes in living cells.

The Zeiss LSM 880 with fast Airyscan confocal microscope is the essential confocal platform for live cell imaging. The unique combination of the Airyscan detector module with the Definite Focus.2 and Z-Piezo drive offers unrivalled flexibility in fast, high resolution live cell imaging.

The system is compatible with most sample holders and contains all necessary accessories for environmental control (for example, CO2, humidity, and temperature control).

Equipment booking

Above: Magnified snapshot of a mammary gland during lactation. The yellow patterns are tiny muscles that contract and release, helping to squeeze milk into the mammary ducts. Purple flecks in and around the structures are immune cells on high alert for any threats in order to help keep the breast tissue healthy. Credit: Caleb Dawson

Above: A Zeiss LSM 880 microscope at WEHI

Hardware

Light sources

Diode laser – 405 nm
Argon laser – 458 nm, 488 nm and 514 nm
Diode pumped solid state laser – 561 nm
HeNe Laser – 594 nm
HeNe laser – 633 nm
HAL 100 Illuminator
HXP-120 120 V

Detection sources

32 channel GaAsP array detection
2 x MA-PMT
Airyscan detector
Transmitted-PMT

Magnification	10x	20x	40x	63x	63x
NA	0.45	0.8	1.3	1.4	1.2
Immersion media	Air	Air	Oil	Oil	Water
XY-resolution (µm) (@ 488 nm)	0.661	0.372	0.229	0.212	0.248
Z-resolution (µm) (@ 488 nm)	4.819	1.524	0.876	0.755	0.902
Working distance (mm)	2.000	0.550	0.21	0.140	0.28

Technological specifications (capabilities)

Available modalities

Z-stack
Time-series
Tile scan
Multi positions
Spectral linear unmixing
Online fingerprinting
Single point FCS
FRAP
Airyscan detection
Fast Airyscan

Additional hardware

Z-Piezo drive
Definite Focus.2

Unique features

The Zeiss LSM 880 combines the fast Airyscan modality with the Z-Piezo drive system to offer fast live cell three-dimensional imaging.
The Definite Focus.2 module provides one of the most robust systems for live time-lapse imaging.