Optical imaging, which includes confocal microscopy, is routinely used in biomedical research for probing cellular morphology, tissue structure and function. A unique feature of this method is the ability to visualise discrete details in the intact biological system across a broad range of scales in both space and time. We specialise in the capture and quantitative analysis of multi-dimensional images, ranging from the subcellular to whole organism level, with a major focus on applying these methods to studies on important diseases, particularly those involving blood and stem cell biology.

The capture and analysis of multi-dimensional data is a multi-stage process, incorporating:
* Experimental design and sample preparation
* Image acquisition
* Image processing and analysis

We work together with researchers with the aim to apply and optimise optical imaging experiments at each stage of this process, while maintaining an emphasis on training and education.


Selected publications from Prof Kelly Rogers

Potts KS, Sargeant TJ, Markham JF, Shi W, Biben C, Josefsson EC, Whitehead LW, Rogers KL, Liakhovitskaia A, Smyth GK, Kile BT, Medvinsky A, Alexander WS, Hilton DJ, Taoudi S. A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. Blood. 2014 Jul 25. pii: blood-2014-02-559468. [Epub ahead of print] PubMed PMID: 25079356.

Kan A, Tan YH, Angrisano F, Hanssen E, Rogers KL, Whitehead L, Mollard VP, Cozijnsen A, Delves MJ, Crawford S, Sinden RE, McFadden GI, Leckie C, Bailey J, Baum J. Quantitative analysis of Plasmodium ookinete motion in three dimensions suggests a critical role for cell shape in the biomechanics of malaria parasite gliding motility. Cell Microbiol. 2014 May;16(5):734-50. doi: 10.1111/cmi.12283. Epub 2014 Mar 28. PubMed PMID: 24612056.

Riglar DT, Rogers KL, Hanssen E, Turnbull L, Bullen HE, Charnaud SC, Przyborski J, Gilson PR, Whitchurch CB, Crabb BS, Baum J, Cowman AF. Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes. Nat Commun. 2013;4:1415. doi: 10.1038/ncomms2449. PubMed PMID: 23361006; PubMed Central PMCID: PMC3562467.

Dragavon J, Blazquez S, Rekiki A, Samson C, Theodorou I, Rogers KL, Tournebize R, Shorte SL. In vivo excitation of nanoparticles using luminescent bacteria. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8890-5. doi: 10.1073/pnas.1204516109. Epub 2012 May 21. PubMed PMID: 22615349; PubMed Central PMCID: PMC3384150.

Josefsson EC, James C, Henley KJ, Debrincat MA, Rogers KL, Dowling MR, White MJ, Kruse EA, Lane RM, Ellis S, Nurden P, Mason KD, O’Reilly LA, Roberts AW, Metcalf D, Huang DC, Kile BT. Megakaryocytes possess a functional intrinsic apoptosis pathway that must be restrained to survive and produce platelets. J Exp Med. 2011 Sep 26;208(10):2017-31. doi: 10.1084/jem.20110750. Epub 2011 Sep 12. PubMed PMID: 21911424; PubMed Central PMCID: PMC3182050.

Riglar DT, Richard D, Wilson DW, Boyle MJ, Dekiwadia C, Turnbull L, Angrisano F, Marapana DS, Rogers KL, Whitchurch CB, Beeson JG, Cowman AF, Ralph SA, Baum J. Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte. Cell Host Microbe. 2011 Jan 20;9(1):9-20. doi: 10.1016/j.chom.2010.12.003. PubMed PMID: 21238943.

Roncali E, Savinaud M, Levrey O, Rogers KL, Maitrejean S, Tavitian B. New device for real-time bioluminescence imaging in moving rodents. J Biomed Opt. 2008 Sep-Oct;13(5):054035. doi: 10.1117/1.2976426. PubMed PMID: 19021415.

Rogers KL, Martin JR, Renaud O, Karplus E, Nicola MA, Nguyen M, Picaud S, Shorte SL, Brûlet P. Electron-multiplying charge-coupled detector-based bioluminescence recording of single-cell Ca2+. J Biomed Opt. 2008 May-Jun;13(3):031211. doi: 10.1117/1.2937236. PubMed PMID: 18601535.

Rogers KL, Picaud S, Roncali E, Boisgard R, Colasante C, Stinnakre J, Tavitian B, Brûlet P. Non-invasive in vivo imaging of calcium signaling in mice. PLoS One. 2007 Oct 3;2(10):e974. PubMed PMID: 17912353; PubMed Central PMCID: PMC1991622.

Rogers KL, Stinnakre J, Agulhon C, Jublot D, Shorte SL, Kremer EJ, Brûlet P. Visualization of local Ca2+ dynamics with genetically encoded bioluminescent reporters. Eur J Neurosci. 2005 Feb;21(3):597-610. PubMed PMID: 15733079

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