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Areas
  • Fundamental biology
  • Biologics
  • Signal transduction
  • Structural biology

About

As a leading expert in kinase structural biology and drug discovery, my lab investigates the complex intracellular communication networks orchestrated by the protein kinase family, with a particular focus on the non-catalytic signalling functions of kinase and pseudokinase scaffolds.

We employ a multidisciplinary approach, combining kinase biochemistry, structural biology, high-throughput screening, proteomics, advanced imaging, chemical biology, and de novo protein design to gain high-resolution insights into these signalling proteins.

Our aim is to unravel deregulation mechanisms, develop innovative targeting strategies, and translate key signalling signatures into novel diagnostics and therapies for aggressive cancers, inflammatory conditions, and neurological disorders.

Publications

Selected publications from Prof Isabelle Lucet

Roy MJ, Surudoi MG, Kropp A, Hou J, Dai W, Hardy JM, Liang L-Y, Cotton TR, Lechtenberg BC, Dite TA, Ma X, Daly RJ, Patel O, Lucet IS. Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling. Nature Communications. 2023;14(1):10.1038/s41467-023-38869-9

Lucet IS, Daly RJ. View from the PEAKs: Insights from structural studies on the PEAK family of pseudokinases. Current Opinion in Structural Biology. 2024;89:10.1016/j.sbi.2024.102932

Afshar-Sterle S, Carli ALE, O’Keefe R, Tse J, Fischer S, Azimpour AI, Baloyan D, Elias L, Thilakasiri P, Patel O, Ferguson FM, Eissmann MF, Chand AL, Gray NS, Busuttil R, Boussioutas A, Lucet IS, Ernst M, Buchert M. DCLK1 induces a pro-tumorigenic phenotype to drive gastric cancer progression. Science Signaling. 2024;17(854):10.1126/scisignal.abq4888

Diplock N, Baudin M, Xiang X, Liang L-Y, Dai W, Murphy JM, Lucet IS, Hassan JA, Lewis JD. Molecular dissection of the pseudokinase ZED1 expands effector recognition to the tomato immune receptor ZAR1. Plant Physiology. 2024;196(1):10.1093/plphys/kiae268

Liang L-Y, Geoghegan ND, Mlodzianoski M, Leis A, Whitehead LW, Surudoi MG, Young SN, Janes P, Shepherd D, Ghosal D, Rogers KL, Murphy JM, Lucet IS. Co-clustering of EphB6 and ephrinB1 in trans restrains cancer cell invasion. Communications Biology. 2024;7(1):10.1038/s42003-024-06118-4

Roy MJ, Surudoi M, Kropp A, Hou J, Dai W, Hardy JM, Liang L-Y, Cotton TR, Lechtenberg BC, Dite TA, Ma X, Daly RJ, Patel O, Lucet IS. Structural mapping of the PEAK pseudokinase interactome identifies 14-3-3 as a molecular switch regulating PEAK3/Crk signalling. Acta Crystallographica Section A: Foundations and advances. 2023;79(a2):10.1107/s2053273323086850

Horne CR, Oliver MR, Shrestha S, Keown JR, Liang L-Y, Young SN, Sandow JJ, Webb AI, Goldstone DC, Lucet IS, Kannan N, Metcalf P, Murphy JM. It takes two to tango: structure of viral kinase PK-1 reveals a new mode of kinase regulation. Acta Crystallographica Section A: Foundations and advances. 2023;79(a2):10.1107/s2053273323087715

Ameen SS, Griem-Krey N, Dufour A, Hossain MI, Hoque A, Sturgeon S, Nandurkar H, Draxler DF, Medcalf RL, Kamaruddin MA, Lucet IS, Leeming MG, Liu D, Dhillon A, Lim JP, Basheer F, Zhu H-J, Bokhari L, Roulston CL, Paradkar PN, Kleifeld O, Clarkson AN, Wellendorph P, Ciccotosto GD, Williamson NA, Ang C-S, Cheng H-C. N-Terminomic Changes in Neurons During Excitotoxicity Reveal Proteolytic Events Associated With Synaptic Dysfunctions and Potential Targets for Neuroprotection. Molecular & Cellular Proteomics. 2023;22(5):10.1016/j.mcpro.2023.100543

Carli ALE, Hardy JM, Hoblos H, Ernst M, Lucet IS, Buchert M. Structure-Guided Prediction of the Functional Impact of DCLK1 Mutations on Tumorigenesis. Biomedicines. 2023;11(3):10.3390/biomedicines11030990

Patel O, Brammananth R, Dai W, Panjikar S, Coppel RL, Lucet IS, Crellin PK. Crystal structure of the putative cell-wall lipoglycan biosynthesis protein LmcA from Mycobacterium smegmatis. Acta Crystallographica Section D, Structural Biology. 2022;78(Pt 4):10.1107/s2059798322001772

Lab research projects

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