Cells are the fundamental building blocks of our body. Diverse types of cells with distinct gene expression profiles together orchestrate the functions of tissues or drive disease pathology. A number of technologies have emerged recently to measure cellular transcriptomic and proteomic information with subcellular resolution. However, these methods have only been successfully applied to thin tissue sections, which are not representative of tissues in the 3-dimensional context.
This project aims to develop and refine a method to enable 3D multiplexed imaging of biomolecules in healthy and pathological tissues (i.e. lung, bone marrow) with subcellular precision. The resulting organ-scale multiomics analysis will advance our understanding of basic biology and the origin for tissue heterogeneity. Students will combine molecular techniques, tissue processing and advanced imaging.
The Centre for Dynamic Imaging is a leading advanced microscopy facility for biomedical research. We are a multidisciplinary team of scientists who specialise in confocal, light sheet, lattice light sheet and super-resolution microscopy techniques and image analysis. We develop and apply state-of-the-art microscopy techniques to tackle challenging medical problems.
This project will involve collaborations with the antibody production and advanced genomics facility at WEHI.