Zeiss Lattice Lightsheet (pre-serial instrument)

The Zeiss Lattice Lightsheet is the first pre-commercial system for lattice light sheet microscopy, enabling a greater number of researchers to access the current state of the art technology for live-cell imaging.

Lattice light sheet microscopy is at the cutting edge of technology for live cell imaging due to its combination of high spatial and temporal resolution whilst simultaneously being incredibly gentle on the samples that are imaged.

This technology is now available as a pre-commercial platform offering researchers experimental flexibility in an easy to use system. The technology offers the ability to interrogate complex biological dynamics in 4D with high resolution over long time periods.

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Hardware

Objectives

Excitation objective

Zeiss 13.3x Magnification, 0.44 NA

Detection Objective

Zeiss 44.93x, 1 NA

Imaging characteristics

44.93x Magnification
XY Resolution – 330 nm
Z resolution – 650 nm
Light sheet length – 10 um – 100 µm

Light sources

Diode lasers lines – 488, 561 and 640 nm

Detection source

PCO.edge sCMOS
2048 x 2048 Pixels
6.5 µm x 6.5 µm pixel size
16 – bit
100 fps (At full frame)
82% QE (Peak at 560 nm)

Technological specifications (Capabilities)

Available modalities

Z-stack
Time Series
Multi-positions

Unique features

Compatible with standard sample chambers such as chamber slides, dishes and multi-well plates.
Contains transmitted light source for quick and easy sample finding.
Can cover a large field of view in a short time.