Centre for Dynamic Imaging

Nikon N-STORM 5.0

A super-resolution fluorescence microscope that localises single molecules in three dimensions to achieve resolutions roughly ten times better than conventional techniques.

The Nikon N-STORM is a single molecule localisation microscope that is capable of imaging in three-dimensions (3D) with resolutions approximately ten times better than conventional microscopy, typically ~20 nm in xy and ~50 nm in z. The system contains Mic-AO, an adaptive optics module to remove optical aberrations induced by thick samples.

The microscope is also capable of multi-colour, total internal reflection fluorescence (TIRF) and live cell imaging.

The system is compatible with standard slides, 35 mm dishes and 8-well chamber slides. It contains all necessary accessories for environmental control; CO2, humidity, and temperature control.

Staff at the Centre for Dynamic Imaging can provide expert guidance on the preparation of samples and choice of fluorophores.


Light sources

  • 405 nm, 488 nm, 568, 640 nm lasers


  • sCMOS cameras (Hamamatsu Orca-Flash4.0 V3)
Immersion mediaOilSilicone Oil
Working distance (mm)0.120.13

Technological specifications (capabilities)

Available modalities

  • 3D Single Molecule Localization (SML), (also known as PALM, FPALM, STORM, dSTORM, etc)
  • 2D SML
  • Multi-colour SML
  • Z-stacks for thick imaging
  • Fixed cell imaging
  • Live cell imaging
  • Tissue imaging
  • Perfect Focus for drift-free imaging

Unique features

  • The N-STORM is a stable microscope platform, which enables super-resolution imaging in cells and tissues.
  • The Mic-AO adaptive optics system removes optical aberrations to achieve more consistent and uniform resolution throughout thick samples.
  • The system enables multi-colour, 3D imaging.
  • A 100x/1.45 Silicone Oil objective is also available. Silicone Oil has a refractive index very close to biological specimens.


  • Ten to hundreds of thousands of camera frames need to be captured to create one super resolved image.
  • Weak staining, excessive bleaching or high levels of background can impair localisation.


  • Staff in the Centre for Dynamic Imaging can provide documentation to assist with the preparation of samples.

Related microscope


Dr Michael Mlodzianoski
Super-resolution specialist
Contact us

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.