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Centre for Dynamic Imaging

Nikon N-STORM 5.0

A super-resolution fluorescence microscope that localises single molecules in three dimensions to achieve resolutions roughly ten times better than conventional techniques.

The Nikon N-STORM is a single molecule localisation microscope that is capable of imaging in three-dimensions (3D) with resolutions approximately ten times better than conventional microscopy, typically ~20 nm in xy and ~50 nm in z. The system contains Mic-AO, an adaptive optics module to remove optical aberrations induced by thick samples.

The microscope is also capable of multi-colour, total internal reflection fluorescence (TIRF) and live cell imaging.

The system is compatible with standard slides, 35 mm dishes and 8-well chamber slides. It contains all necessary accessories for environmental control; CO2, humidity, and temperature control.

Staff at the Centre for Dynamic Imaging can provide expert guidance on the preparation of samples and choice of fluorophores.

Objectives

Light sources

  • 405 nm, 488 nm, 568, 640 nm lasers

Cameras

  • sCMOS cameras (Hamamatsu Orca-Flash4.0 V3)
Magnification100x100x
NA1.491.45
Immersion mediaOilSilicone Oil
Working distance (mm)0.120.13

Technological specifications (capabilities)

Available modalities

  • 3D Single Molecule Localization (SML), (also known as PALM, FPALM, STORM, dSTORM, etc)
  • 2D SML
  • TIRF SML
  • Multi-colour SML
  • Z-stacks for thick imaging
  • Fixed cell imaging
  • Live cell imaging
  • Tissue imaging
  • Perfect Focus for drift-free imaging

Unique features

  • The N-STORM is a stable microscope platform, which enables super-resolution imaging in cells and tissues.
  • The Mic-AO adaptive optics system removes optical aberrations to achieve more consistent and uniform resolution throughout thick samples.
  • The system enables multi-colour, 3D imaging.
  • A 100x/1.45 Silicone Oil objective is also available. Silicone Oil has a refractive index very close to biological specimens.

Limitations

  • Ten to hundreds of thousands of camera frames need to be captured to create one super resolved image.
  • Weak staining, excessive bleaching or high levels of background can impair localisation.

Resource

  • Staff in the Centre for Dynamic Imaging can provide documentation to assist with the preparation of samples.

Related microscope

Contact us

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.