Leica Stellaris 8

Leica Stellaris 8 is our most recent confocal microscope with multiple advanced modalities, such as live cell imaging, fluorescence lifetime imaging (FLIM), SpectraPlex high-plex imaging and LIGHTNING deconvolution super-resolution imaging. Its strengths span multiple areas such as high speed, high resolution, high sensitivity and flexible signal detection, temporally resolved signal unmixing (for signal with overlapping emission spectra), 3D high-multiplexing (up to 15-plex), as well as gentle yet precise Z-stacking.

Magnification	Numerical Aperture (NA)	Immersion	Free Working Distance (um)	XY Resolution at 488 nm (um)	Z Resolution at 488 nm (um)	Cover glass thickness (mm)
20x	0.75	Air (Dry)	620	260.27	648.63	0.17 (#1.5)
40x	1.3	Oil	240	150.15	299.1	0.17 (#1.5)
63x	1.4	Oil	140	139.43	235.82	0.17 (#1.5)
86x	1.2	Water	300	162.67	290.28	0.14-0.19 (#1-1.5)

Name	Spectral Range (nm) of Highest Sensitivity Relative to Other HyDs	Best for
HyD S	<550	General fluorescence, photon-counting
HyD X	550-750	Weak fluorescent signal, FLIM, photon-counting, high temporal resolution imaging
HyD R	750-825	Far-red fluorophores
Trans PMT	400-750	Transmitted light imaging e.g. brightfield

Other specifications

Model: DMi8 microscope
Acousto-Optical Beam Splitter (AOBS): allows fast flexible and precise wavelength transmission without switching hardware; offers better excitation/emission separation than traditional beam splitting mirrors
Live imaging incubation chamber and component control unit: CO2, humidity and temperature controlled
Super Z Galvo stage: high speed, low vibrations, and high precision for real-time Z-stacking
USB Control panel: allows quick adjustment on users’ pre-defined settings e.g. smart intensity, gain, zoom factor, pinhole, and Z position etc.

Unique features

Leica Stellaris 8 is our most versatile confocal microscope available at the CDI, with multiple attractive features that push the boundaries of conventional confocal microscopy.

It enables fast, high-resolution visualization of both fixed and live samples in multiple colors, while minimizing crosstalk and hardware-related artifacts.

This ensures high-quality results and empowers researchers to explore unprecedented temporal and spatial information in 3D.

Contact the Centre for Dynamic Imaging to book equipment or discuss imaging collaborations.

imaging@wehi.edu.au

Centre for Dynamic Imaging