Our research is dedicated to understanding the mechanisms that regulate cell death and inflammation in the human body. When cells die, they release inflammatory molecules contributing to numerous inflammatory diseases. Using proteomics, a comprehensive method for studying proteins, we have identified proteins released by dead cells and those that regulate cell death. This new knowledge holds great promise for creating novel treatment approaches to manage inflammation.
We are also dedicated to exploring dead cell removal by phagocytes. This process is essential for reducing inflammation because it removes dead cells and leads to the release of anti-inflammatory molecules. By employing proteomics, we aim to better understand how this process functions so that we can develop methods to control it, ultimately improving wound healing and decreasing inflammatory disease.
At our laboratory, we are committed to unveiling disease-relevant, druggable mechanisms that regulate cell death. To accomplish this goal, we are developing cutting edge proteomics techniques and integrating them with cell and molecular biology approaches.
My previous work during my PhD and Postdoc uncovered cell death mechanisms and signalling events that regulate inflammation, presenting innovative therapeutic approaches to treat inflammatory disease.
These studies laid the groundwork for developing cell death inhibitors, which are currently in the pre-clinical selection phase.
The efficient engulfment of dead cells by macrophages is crucial for preventing the release of inflammatory molecules into the environment.
This project aims to analyse the signalling events driving the engulfment process and the resulting reprogramming of macrophages, which leads to the release of anti-inflammatory cytokines. To achieve this, we will combine the analysis of post-translational modifications (phosphorylation and ubiquitylation) using high-sensitivity proteomics with CRISPR-Cas9 technology.
Macrophages play a vital role in wound healing by removing dead cells and releasing molecules that reduce inflammation and stimulate fibroblasts to produce extracellular matrix. We will, for the first time, comprehensively analyse proteins released by engulfing macrophages, investigating their release mechanisms and specific roles in wound healing. Moreover, we will examine the impact of dead cell clearance on antigen presentation.
Several studies investigating dead cell clearance indicate the leaking of lysosomal content into the cytosol. This can have dramatic effects on macrophage programming. With this project, we employ a combination of microscopy and proteomic techniques to investigate the mechanism of lysosomal leakage and its effect on cell signalling and macrophage reprogramming.
Several studies on dead cell clearance indicate lysosomal content leakage into the cytosol, which can have dramatic effects on macrophage programming.
In this project, we will employ a combination of microscopy and proteomic techniques to investigate the mechanism of lysosomal leakage and its effect on cell signalling and macrophage reprogramming.
We are a dynamic and driven team employing cutting-edge proteomics to investigate inflammation and its resolution.
Our laboratory is integrated within the Advanced Technology and Biology division and the Inflammation division, providing the ideal environment for the technology we employ and the biological questions we strive to answer.