Oblique Plane Lightsheet Microscope

Description: Oblique Plane Light Sheet Microscopy (OPLSM) is an advanced imaging technique that uses an angled light sheet to illuminate a single plane within a sample, enabling fast, high-contrast volumetric imaging with minimal photodamage.

The SCAPE 2.0 system developed by the Hillman lab is a leading example of OPLSM, offering rapid 3D imaging of living tissues and organisms at high spatiotemporal resolution. This approach allows researchers to capture dynamic biological processes in real time across large volumes with unprecedented speed and clarity.

To book, please contact Dr Emma Gill.

Objectives

Set 1:

O1: Olympus XLUMPlanFL N, 20x, NA1.00 W, inf/0/FN22
O2: Nikon F FL, 20x, NA0.75, WD 1.0, Working distance 4.5mm
O3: Nikon M Plan Apo HR, 50x, NA0.75, inf/0 f=200, Working distance:5.2mm

Light sources:

Diode laser – 405 nm, 120 mW
CW Ytterbium Fiber Laser – 488 nm, 500 mW
CW Ytterbium Fiber Laser – 561 nm, 500 mW
CW Ytterbium Fiber Laser- 647 nm, 500 mW

Detectors:

ORCA-Quest, qCMOS camera 15550, SDR Scientific Hamamatsu

Image Processing

Napari plugin for custom analysis and visualization of lattice lightsheet and Oblique Plane Microscopy data.

The plugin is optimized for data from the Zeiss lattice lightsheet microscope.

Access the plugin

Unique Features

Unlike traditional point-scanning methods, SCAPE 2.0 utilises a single, stationary objective lens and a swept light sheet to rapidly capture three-dimensional data without the need for mechanical scanning, significantly reducing photodamage and photobleaching.

Limitations

A limitation of SCAPE 2.0 is that it is not yet commercially available and requires specialised analysis software, which can limit accessibility and ease of use for many laboratories. This custom setup demands technical expertise for both operation and data processing.

Centre for Dynamic Imaging