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Diseases
  • Head and neck cancer
  • Autoinflammatory disease
Technologies
  • Molecular biology
  • Mouse genetics
Divisions

About

My lab is interested in how cell death and inflammation are regulated. We focus on three key families of molecules:

* The tumor necrosis factor super family (TNFSF) that regulates the immune system and inflammation.
* Ubiquitin E3 ligases that play an important role regulating signalling from the TNFSF and other critical immune regulators.
* RIP Kinases, which play an important role in effecting inflammation, apoptotic and necroptotic cell death.

My lab uses a wide array of approaches including biochemistry, cell biology, small molecules and genetics so that discoveries made at the molecular level can be transposed into physiological findings in vivo. Using this approach I hope we can make medically relevant discoveries that can be translated into human treatments.

Publications

Selected publications from Prof John Silke

Lelliott EJ, Naddaf J, Ganio K, Michie J, Wang S, Liu L, Silke N, Ahn A, Ramsbottom KM, Brennan AJ, Freeman AJ, Goel S, Vervoort SJ, Kearney CJ, Beavis PA, McDevitt CA, Silke J, Oliaro J. Intracellular zinc protects tumours from T cell-mediated cytotoxicity. Cell Death & Differentiation. 2024;31(12):10.1038/s41418-024-01369-4

Anderton H, Bandala-Sanchez E, Simpson DS, Rickard JA, Ng AP, Di Rago L, Hall C, Vince JE, Silke J, Liccardi G, Feltham R. Correction to: RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development. Cell Death & Differentiation. 2024;31(12):10.1038/s41418-024-01401-7

Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P-C, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M. Spatial proteomics identifies JAKi as treatment for a lethal skin disease. Nature. 2024;635(8040):10.1038/s41586-024-08061-0

Liu L, Silke J. AXIN2 is a non‐redundant regulator of AXIN1 stability and β‐catenin in colorectal cancer cells. The FEBS Journal. 2024;:10.1111/febs.17336

Wang Y, Liu Z, Si Q, Lu W, Song Y, Jin W, Yang X, Li Z, Hu X, Ding L, Jing Y, Weng P, Yu Q, O’Reilly LA, Silke J, Zhang X, Hu Q, Ni Y. Complex IIa formation and ABC transporters determine sensitivity of OSCC to Smac mimetics. Cell Death & Disease. 2024;15(11):10.1038/s41419-024-07253-w

Nordmann T, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler P, Sinha A, Andreas M, Florian R, Maximilian Z, Satoh TK, Saito Y, Gong T, Thielert M, Kimura H, Nguyen H, Levesque M, Murray P, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M. 720 Cell-type resolved spatial proteomics uncovers a novel actionable therapeutic target in toxic epidermal necrolysis. Journal of Investigative Dermatology. 2024;144(8):10.1016/j.jid.2024.06.736

Chiou S, Al-Ani AH, Pan Y, Patel KM, Kong IY, Whitehead LW, Light A, Young SN, Barrios M, Sargeant C, Rajasekhar P, Zhu L, Hempel A, Lin A, Rickard JA, Hall C, Gangatirkar P, Yip RK, Cawthorne W, Jacobsen AV, Horne CR, Martin KR, Ioannidis LJ, Hansen DS, Day J, Wicks IP, Law C, Ritchie ME, Bowden R, Hildebrand JM, O’Reilly LA, Silke J, Giulino-Roth L, Tsui E, Rogers KL, Hawkins ED, Christensen B, Murphy JM, Samson AL. An immunohistochemical atlas of necroptotic pathway expression. EMBO Molecular Medicine. 2024;16(7):10.1038/s44321-024-00074-6

Meier P, Legrand AJ, Adam D, Silke J. Immunogenic cell death in cancer: targeting necroptosis to induce antitumour immunity. Nature Reviews Cancer. 2024;24(5):10.1038/s41568-024-00674-x

Tran HT, Kratina T, Coutansais A, Michalek D, Hogan BM, Lawlor KE, Vince JE, Silke J, Lalaoui N. RIPK3 cleavage is dispensable for necroptosis inhibition but restricts NLRP3 inflammasome activation. Cell Death & Differentiation. 2024;31(5):10.1038/s41418-024-01281-x

Oda H, Manthiram K, Chavan PP, Rieser E, Veli Ö, Kaya Ö, Rauch C, Nakabo S, Kuehn HS, Swart M, Wang Y, Çelik NI, Molitor A, Ziaee V, Movahedi N, Shahrooei M, Parvaneh N, Alipour-olyei N, Carapito R, Xu Q, Preite S, Beck DB, Chae JJ, Nehrebecky M, Ombrello AK, Hoffmann P, Romeo T, Deuitch NT, Matthíasardóttir B, Mullikin J, Komarow H, Stoddard J, Niemela J, Dobbs K, Sweeney CL, Anderton H, Lawlor KE, Yoshitomi H, Yang D, Boehm M, Davis J, Mudd P, Randazzo D, Tsai WL, Gadina M, Kaplan MJ, Toguchida J, Mayer CT, Rosenzweig SD, Notarangelo LD, Iwai K, Silke J, Schwartzberg PL, Boisson B, Casanova J-L, Bahram S, Rao AP, Peltzer N, Walczak H, Lalaoui N, Aksentijevich I, Kastner DL. Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency. Nature Immunology. 2024;25(5):10.1038/s41590-024-01817-w

Lab research projects

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