WEHI researchers have discovered how dormant Toxoplasma parasites in the brain manipulate their host cells to ensure their own survival.
Associate Professor Chris Tonkin (L) and Dr Simona Seizova led research that showed how dormant Toxoplasma parasites manipulate host cells to survive.
The researchers showed that the parasites were able to lay dormant and undetected inside neurons (brain cells) and muscle cells by releasing proteins that switch off the cells’ ability to alert the immune system. With expertise and technologies from WEHI’s Advanced Genomics facility and Centre for Dynamic Imaging, they were able to visualise the parasites in real-time.
The discovery provides a better understanding of how Toxoplasma parasites can hide in the brain and what triggers them to reactivate and cause disease. It could pave the way for new drug targets to treat patients suffering from chronic toxoplasmosis infections.
Researchers have discovered how dormant Toxoplasma gondii parasites manipulate host cells in the brain to foster their own survival.
They showed that dormant parasites (called bradyzoites) exported proteins to silence immune signalling in infected host cells and hide undetected.
The discovery could pave the way for new treatments for chronic toxoplasmosis infections.
A ‘molecular battle’ for survival
Toxoplasmosis is a parasitic infection that affects humans and other mammals. It is caused by eating undercooked or contaminated meat, and from exposure to infected cat faeces.
Around one in five Australians have been infected with Toxoplasma gondii, the parasite that causes toxoplasmosis. Toxoplasma infections can cause congenital birth defects, blindness and neurological dysfunction in unborn children. In adults who are immunocompromised, it can cause blindness and even death.
Dr Seizova, who undertook the research as her PhD project at WEHI, said research in the area had been limited due to the complexity of working with the parasite during chronic stages of infection.
“However with an interdisciplinary team of dedicated researchers and the state-of-the-art facilities at WEHI, we were able to start unpacking Toxoplasma’s secrets,” Dr Seizova said.
“Our results were surprising. The field has previously described the parasite as dormant in the chronic stages; however we showed that bradyzoites aren’t silent players. The parasites continue to manipulate their host and protect themselves from immune attack long past the initial stages of infection.
She said the most exciting part of the discovery was that it has opened the door to a new area of research in the field.
“Toxoplasma is a fascinating parasite and there is much more to learn about these chronic stages of infection and their dance with the immune system.”
PhD student Ushma Ruparel said Toxoplasma parasites often lay dormant in the brain of the host animal, where they could reactivate to cause severe disease.
“We know that Toxoplasma parasites make their own molecules and proteins that it exports into the host cell to protect itself from detection by the immune system. While this is well-understood in the acute, initial stage of infection, how Toxoplasma hijacks its host cells in its dormant form was unknown,” Ms Ruparel said.
“With our colleagues who have expertise in genomics and advanced imaging, we showed that the parasite exports proteins called Inhibitor of STAT1 transcription (IST) to the host cell to suppress immune signals.”
Associate Professor Tonkin said IST played a key role in limiting interferon signalling in bradyzoites, to protect host cells from immune-mediated cell death, which is essential to the body’s fight against Toxoplasma.
“Interferon is the beacon molecule of the immune system. It gives off a blinking sign to notify the immune system that the body has been infected with a range of diseases, including Toxoplasma,” he said.
“Interferon is crucial in the fight against disease. However, the cunning parasite tries to turn this signal off during the latent period, as this is the only way it can survive. While the immune system is determined to rid the infection from the body, the parasite only has survival on its mind. So, it’s essentially a tug-of-war, a molecular battle.”
Link to neurological disease
A Toxoplasma-infected human cell moments before it bursts. The golden orbs are sacs filled with hundreds of parasites that will eventually burst free and infect neighbour cells (blue). Credit: Simona Seizova/WEHI
While the exact mechanism of why and how the pathogen is reactivated to create disease is little understood, there appears to be a correlation between people who have immune deficiencies and brain lesions.
The association of Toxoplasma with neuropsychiatric conditions over the past decade is another curious element to this disease, said Associate Professor Tonkin.
“While there is a correlation between Toxoplasma infection and schizophrenia, bipolar disorder and other neurodegenerative conditions like Alzheimer’s disease, there’s no causation yet as we don’t know the cause and effect,” Associate Professor Tonkin said.
“The way this parasite resides in our brains during chronic infection and the way that it shuts down our innate immune system to survive is quite unique. While this work was all done in the laboratory, it will lay the foundation to bring us closer to understanding these age-old questions about how these pathogens interfere in our neural pathways and what their exact role is.
“Our next step is to figure out what it is about chronic infections that make those changes really unique to chronic infections. We have found one specific parasite protein that seems to have a role, but it doesn’t seem to account for all changes, and we want to explore if there are any others.”
Tackling a key health burden
The findings have started to unravel the molecular players that help pathogens create latency, which could pave the way for a better understanding of the impact of parasites on the brain.
Associate Professor Tonkin said latent infections were one of the biggest global health burdens.
“Diseases such as HIV, hepatitis, malaria and tuberculosis are all examples of chronic or latent infections that cause significant disease, disability and economic impact. If we can understand what causes these latent infections to reactivate and their control over our cells and brain, we will be on the pathway to finding new treatments that diminish this burden for some of the most underprivileged people around the world.”
This research was supported by the Australian National Health and Medical Research Council, the David Winston Turner Endowment Fund and the Victorian Government.
Dr Seizova is now a postdoctoral researcher at the University of Dundee, Scotland.
WEHI authors
Simona Seizova, Ushma Ruparel, Alexandra Garnham, Stefanie Bader, Alessandro D Uboldi, Michael Coffey, Lachlan Whitehead, Kelly Rogers, Christopher Tonkin.
