WEHI Wednesday Seminar hosted by Dr Lachlan Whitehead
 

Dr Pradeep Rajasekhar

Senior Research Officer – Centre for Dynamic Imaging, Advanced Technology & Biology division, wehi

Morphodynamics, not morphology: getting more from 4D microscopy data

Davis Auditorium

Join via SLIDO enter code #WEHIWednesday

Including Q&A session

When we observe living cells under a microscope, we capture hours of dynamic behaviour but typically reduce this to static measurements. How much of the information are we throwing away? Using 4D lattice light-sheet microscopy, we tracked thousands of neutrophils as they underwent NETosis, a form of immune cell death. We measured how cell morphology changes over time and uncovered  treatment effects that were not immediately apparent to conventional endpoint analysis. Different stimuli don’t just push cells to  different endpoints; they change how cells get there. We present a framework for extracting temporal information that can be applied to other live imaging experiments and introduce early work with self-supervised deep learning to push these approaches further.

All welcome!