WEHI Wednesday Seminar hosted by Professor Warren Alexander
 

Dr Carolyn de Graff
Senior Research Officer – Strasser Laboratory, Blood Cells & Blood Cancer division  – Cancer Research & Treatments Theme – WEHI

Evolution of haematopoiesis: regulation of gene expression in vertebrate blood cells

 

Davis Auditorium

Join via SLIDO enter code #WEHIWednesday

Including Q&A session
 

The major hematopoietic lineages have existed since early in vertebrate evolution, covering the key functions of oxygen transport, clotting and defense against infection. Some of the specifics of how these functions are performed are unique to mammals, such as using platelets formed from polyploid megakaryocytes for clotting rather than thrombocytes. Using single cell transcriptomes, we have created an atlas of gene expression of haematopoietic cells in mammals, birds and fish, and annotated cells to their closest mammalian homologue by lineage. We have used a mixture of publicly available data and generated our own atlas of chicken haematopoiesis as a representative of bird haematopoiesis. This has allowed us to look at how conserved haematopoietic gene expression is in different vertebrates in relation to human blood cells and find species specific haemopoietic and immune cell types. We have identified genes which are conserved as signatures of blood and immune lineages across all vertebrates and genes which are only associated with mammalian haematopoiesis. We have then investigated the regulatory elements associated with gene expression during haematopoiesis using ATAC-seq data. We have then taken the mammalian specific and vertebrate conserved gene lists and looked at their regulation via ATAC-seq and found that the more broadly conserved genes have more lineage specific open chromatin regions associated with them than genes with mammalian specific gene expression. This suggests that redundancy in regulation helps maintain lineage specific expression in divergent species.

Carolyn de Graaf completed her PhD at WEHI, investigating regulation of gene expression during haematopoeisis, and production of megakaryocytes. She then worked at the Netherlands Cancer Institute in Amsterdam, studying interactions between the genome and the nuclear lamina, and investigating single cell chromatin interactions using DamID. She then returned to WEHI and is currently in the Division of Blood Cells and Blood Cancer. 

All welcome!