Understanding the genetics of neutrophil maturation

Understanding the genetics of neutrophil maturation

Project details

Neutrophils are early responders to inflammation. They contain antimicrobial granules which they release to combat infection. They are also characterised by a polymorphic nucleus which becomes multilobed in mature neutrophils.

We have been conducting an image based CRISPR screen to identify genes that are involved in two key components of neutrophil maturation – granule production and the increasing complexity of nuclear morphology.

In this project, you will characterise hits we have identified from this screen in detail. Firstly, you will use CRISPR and other molecular biology techniques confirm whether the genes have a role in neutrophil maturation. Confirmed hits will be further characterised with microscopy to study morphological defects and in vitro assays to characterise functional defects.

About our research group

Our laboratory studies the development and function of blood cells. We aim to identify genes and molecular pathways that are important in regulating normal blood cell production and understand the changes that lead to blood cell disorders such as autoimmune and inflammatory diseases, leukemia and lymphoma. We work with clinicians and industry partners to find better ways of treating these illnesses.

We take a multidisciplinary approach combining cell biology, physiology, biochemistry and molecular biology, genomics, bioinformatics and computational science to understand the blood cell system and its individual lineages. We also develop software that assists biologist in visualising and interacting with large data sets.

 

Email supervisors

 

Researchers:

Dr Carolyn de Graaf profile photo
Dr
Carolyn
de Graaf
Blood Cells and Blood Cancer division

Professor Doug Hilton

Professor Doug at WEHI
Professor
Doug
Hilton
Institute Director; Laboratory Head

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