Quantitation of human T cell responses in primary immunodeficiency

Quantitation of human T cell responses in primary immunodeficiency

Project details

The balanced interplay of lymphocyte growth, survival and differentiation is essential for a healthy immune system. Upon antigen encounter cells undergo multiple rounds of division, before stopping to divide and eventually die. In patients with immune disorders, this balance is disturbed so that they mediate an immune response that is either too strong (autoimmunity) or insufficient (primary immunodeficiency). This project uses quantitative cellular and molecular assays established in the Hodgkin laboratory (Marchingo Science 2014(346):1123) to measure the regulation of proliferative responses in human T cells to explore a new theory for the emergence of immunodeficiency and autoimmune disorders. Understanding the mechanisms and molecules controlling human T cell responses will help understand the pathophysiology of immune disorders and help develop therapeutics targeting these processes.

About our research group

The principles of cellular calculation that underlie this project were developed in the lab of Prof Phil Hodgkin with the goal of building computer models that can be used to understand and improve treatments for immune disorders. Typical experiments in the lab use flow cytometry and direct video imaging to measure the effect of changing conditions such as cytokines, altered genetic makeup, or the impact of pharmacological agents on individual cells and how they vary in a population. Bringing these principles to human immunology is a long-term goal shared by Dr Bryant and Dr Heinzel, both internationally-trained experts in human immunology, and the research tools they have developed, together with Prof Phil Hodgkin, will provide a highly competitive advantage in investigation of the causes of primary immunodeficiency. 

Researchers:

Vanessa Bryant in the lab
Dr
Vanessa
Bryant
Immunology division
Susanne Heinzel profile
Dr
Susanne
Heinzel
Immunology division

Project Type: