Neutrophil heterogeneity in inflammation

Neutrophil heterogeneity in inflammation

Project details

Neutrophils are the most abundant leukocyte in the blood and the first mobilised to a site of injury or infection where they use various strategies to provide critical first-line defence.  While once considered to be a homogeneous population, recent evidence suggests that neutrophils have different phenotypes and functions depending on the context. Despite the acceptance that neutrophils are heterogenous, few studies have explored how the subsets are different or how they contribute to the inflammatory process.

We will isolate neutrophils from in the blood of healthy people and individuals treated with G-CSF and use single-cell RNA sequencing to identify discrete populations. We will then use flow cytometry and functional immune readouts including cytokine production and cell migration to understand how these populations are different.

About our research group

The Wicks lab is interested in understanding the complex biological changes that occur during inflammatory and autoimmune diseases. A major research theme of my lab is to investigate how the cytokines G-CSF and GM-CSF promote inflammatory disease. This has resulted in collaborations with the biotechnology sector and led to the development of antibody-based therapies that are currently undergoing clinical trials.

We use preclinical models of these human diseases, in parallel with patient samples, to gain a better understanding of the molecular events that drive disease progression and tissue pathology. Through these studies we ultimately hope to unveil critical information that will enable the development of new diagnostic tools and therapies.

Researchers:

Dr Katherine Martin profile photo
Dr
Katherine
Martin
Inflammation division

Professor Ian Wicks

Ian Wicks
Professor
Ian
Wicks
Joint Division Head, Laboratory Head

Project Type: