Giardia duodenalis phosphoproteome and protein kinase network

Giardia duodenalis phosphoproteome and protein kinase network

Project details

Giardia duodenalis is a parasite causing diarrhoeal disease (giardiasis) globally. Giardia has unexplored reliance on protein modifications, including phosphorylation. Phosphorylation is dynamic and reversible, regulating functions of the modified proteins (‘substrate’). Phosphorylation is catalysed by protein ‘kinase’ enzymes, and Giardia possesses the smallest core complement of kinases (~80 kinases) and a unique Nek kinase family (~200 kinases) whose function remains largely unknown (Manning et al, Genome Biology, 2011 12:R66).

This project will use proteomic technologies to enrich phosphorylated protein substrates and define exact phosphorylation sites in these proteins. Processing trophozoites into sub-cellular structures will allow identification of co-occuring protein kinases and their protein substrates. Further opportunities exist for localisation of protein kinases, western-blot screening of cellular phosphorylation and protein kinase drug-inhibition screens.

About our research group

The Jex research group uses advanced sequencing technologies to dissect and understand parasite biology to improve treatment options and outcomes. This research involves the gastrointestinal parasite Giardia duodenalis, which causes significant global disease, has limited treatments, but also possesses a compact genome and in vitro culture system making it a useful model organism. We have ongoing Giardia culture for selection of drug resistance, in vitro inducible stress models and for modelling parasite life cycle. The material generated through culture is sequenced, with lab expertise including quantitative proteomics, transcriptomics and genomics. The lab also collaborates with the Svärd Lab in Uppsala, Sweden, who have extensive expertise in Giardia cell biology, as well as Microbial Screening Technologies, who specialise in microbial natural products and inhibitors for drug screening.

 

Researchers:

Samantha Emery profile photo
Dr
Samantha
Emery
Population Health and Immunity division

Dr Andrew Webb

Dr Andrew Webb in the lab
Dr
Andrew
Webb
Acting Division Head

Project Type: