Eosinophil heterogeneity

Eosinophil heterogeneity

Project details

Our laboratory has performed single cell RNA sequencing on eosinophils isolated from the blood, bone marrow and lung. We will interrogate this sequencing data to find clusters of eosinophils with distinct patterns of gene expression. We will assess cytokine and granule protein expression in the clusters to determine if there are eosinophil subsets with specialized roles such as granule secretion, cytokine secretion, or secretion of different types of cytokine such as Th1 or Th2 cytokines. We will probe the function of these novel subsets of eosinophils using inflammatory models, helminth infections and transfer experiments.

The successful applicant will learn molecular, cellular and quantitative biological methods. They will also have the chance to perform data analysis on cutting edge single cell sequencing data.

About our research group

The Hilton laboratory studies the development and function of blood cells, with a current focus on granulocytes. We aim to identify genes and molecular pathways that are important in regulating normal blood cell production and understand the changes that lead to blood cell disorders such as autoimmunity, inflammatory diseases, and leukemia. We work with clinicians and industry partners to find better ways of treating these illnesses.

We take a multidisciplinary approach combining cell biology, physiology, biochemistry and molecular biology, genomics, bioinformatics and computational science to understand the blood cell system and its individual lineages. We also develop software that assists biologist in visualising and interacting with large data sets. We have a team of talented motivated post-docs ready to teach you the latest techniques.

 

 

Researchers:

Professor Doug Hilton

Professor Doug Hilton in the office
Professor
Doug
Hilton
Institute Director, Division Head
Dr Kirsten Fairfax profile photo
Dr
Kirsten
Fairfax
Molecular Medicine division
Dr Carolyn deGraaf profile photo
Dr
Carolyn
deGraaf
Molecular Medicine division

Project Type: