Eosinophil and neutrophil heterogeneity (Masters option available)

Eosinophil and neutrophil heterogeneity (Masters option available)

Project details

Our laboratory has performed single cell RNA sequencing on eosinophils and neutrophils isolated from various tissues in mice and humans. We will interrogate this sequencing data to find clusters of eosinophils and neutrophils with distinct patterns of gene expression. We will assess cytokine and granule protein expression in the clusters to determine if there are subsets with specialized roles such as granule secretion, cytokine secretion, or secretion of different types of cytokine such as Th1 or Th2 cytokines. We will probe the function of these novel subsets using inflammatory models, helminth infections and transfer experiments. 

The successful applicant will learn molecular, cellular and quantitative biological methods. They will also have the chance to perform data analysis on cutting edge single cell sequencing data. 

About our research group

The Hilton laboratory studies the development and function of blood cells, with a current focus on granulocytes. We aim to identify genes and molecular pathways that are important in regulating normal blood cell production and understand the changes that lead to blood cell disorders such as autoimmunity, inflammatory diseases, and leukemia. We work with clinicians and industry partners to find better ways of treating these illnesses. 

We take a multidisciplinary approach combining cell biology, physiology, biochemistry and molecular biology, genomics, bioinformatics and computational science to understand the blood cell system and its individual lineages. We also develop software that assists biologist in visualising and interacting with large data sets. We have a team of talented motivated post-docs ready to teach you the latest techniques. 


Professor Doug Hilton

Professor Doug Hilton at the Institute
Institute Director; Laboratory Head
Dr Carolyn de Graaf profile photo
de Graaf
Blood Cells and Blood Cancer division
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Blood Cells and Blood Cancer division

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