Developing new tools to visualise necroptotic cell death

Developing new tools to visualise necroptotic cell death

Project details

There is growing evidence that necroptosis - a newly recognised form of cell death - contributes to many human pathologies including cancer, neurodegeneration, autoimmune disease, and tissue ischaemia. However, owing to its recent discovery, there are a lack of methods for identifying “when” and “where” necroptosis is activated during disease. To address this gap, we are developing several microscope-based techniques for the in situ detection of necroptosis. In particular, this project aims to develop novel immunofluorescence reagents, proximity ligation assays and split complementation systems for visualising necroptosis within tissues. 

Development of these advanced tools will provide critical insights into how necroptosis mediates disease. During this project, the student will learn state-of-the-art imaging approaches ranging from fluorescence microscopy of cleared whole tissues to super-resolution microscopy. 

About our research group

Our laboratory is interested in the protein interactions that underpin necroptotic cell death, and how these interactions can be targeted for the development of novel anti-necroptotic therapies to treat human disease. Our studies draw upon diverse methodologies including structural biology, cellular biology, fluorescence microscopy (e.g. tissue clearing, light sheet, multiphoton and super-resolution microscopy) and in vivo models of disease to elucidate the molecular mechanisms of necroptosis. 

 

 

Researchers:

Dr Andre Samson profile photo
Dr
Andre
Samson
Cell Signalling and Cell Death division

Project Type: