Biogenesis of eosinophil granules

Biogenesis of eosinophil granules

Project details

We recently created an atlas of gene expression data covering all hematopoietic lineages, including eosinophils (de Graaf, Stem Cell Reports 2016). We have used this atlas in concert with rational design to select genes that may be involved in eosinophil maturation. 

MAPT, or microtubule-associate protein Tau, is involved in intracellular protein transport and is highly and specifically expressed in eosinophils. We will analyse whether MAPT plays a role in eosinophil granule generation and degranulation. We have developed a retroviral construct to allow us to visualise granule biogenesis in the presence and absence of MAPT. Stable extracellular granules may be a great way to deliver drugs.

The successful applicant will learn molecular, cellular and quantitative biological methods including cloning, qRT-PCR, imaging, subcellular fractionation, FACs and more!

 

About our research group

The Hilton laboratory studies the development and function of blood cells, with a current focus on granulocytes. We aim to identify genes and molecular pathways that are important in regulating normal blood cell production and understand the changes that lead to blood cell disorders such as autoimmunity, inflammatory diseases, and leukemia. We work with clinicians and industry partners to find better ways of treating these illnesses.

We take a multidisciplinary approach combining cell biology, physiology, biochemistry and molecular biology, genomics, bioinformatics and computational science to understand the blood cell system and its individual lineages. We also develop software that assists biologist in visualising and interacting with large data sets. We have a team of talented motivated post-docs ready to teach you the latest techniques.

 

 

Researchers:

Professor Doug Hilton

Professor Doug Hilton in the office
Professor
Doug
Hilton
Institute Director, Division Head
Dr Kirsten Fairfax profile photo
Dr
Kirsten
Fairfax
Molecular Medicine division
Dr Carolyn deGraaf profile photo
Dr
Carolyn
deGraaf
Molecular Medicine division

Project Type: