Robyn McConville - Infectious Diseases & Immune Defence division

Robyn McConville - Infectious Diseases & Immune Defence division

Location: 
Bio21 Auditorium
Start Time: 
Wed, 01/02/2023 - 1:00pm
End Time: 
Wed, 01/02/2023 - 2:00pm

WEHI Wednesday Seminar hosted by Associate Professor Justin Boddey

Robyn McConville

PhD Student - Boddey Laboratory, Infectious Diseases & Immune Defence division – Infection, Inflammation & Immunity Theme, WEHI (this is a PhD Completion seminar)



Dissecting the role of protein export in Plasmodium falciparum liver stages




Bio21 Auditorium

Join via ZOOM

Webinar ID: 850 5905 8387

Passcode: wehiwednes

Including Q&A session



The malaria parasite Plasmodium falciparum undergoes initial replication and development in the liver before establishing bloodstream infections and clinical symptoms of malaria. Plasmodium liver stages reside within a unique parasite-derived compartment known as the parasitophorous vacuole (PV). How and to what extent these parasite stages export proteins into the cytoplasm of the host hepatocytes and acquire nutrients is poorly understood. Recent studies suggest that Plasmodium liver stages express components of the Plasmodium Translocon of EXported Proteins (PTEX) complex that is involved in transporting parasite proteins across the PV membrane of asexual blood stages. To investigate whether the PTEX translocon is functional and important for liver stage development we established the first conditional FLP/FRT recombination system in P. falciparum inducing site-specific recombination during mosquito transit and selective knock-out of the PTEX in liver stage parasites. The in vivo liver development phenotype of PTEX-deficient parasites was assessed in humanised mice and revealed expression is critical for liver stage development. This work will provide new tools for studying the biology of liver stage parasites and the potential role of PTEX in regulating the transport of effector proteins and nutrients in these stages.

 

All welcome!