Research Assistant

Research Assistant

Application closing date: 
Wed, 21/04/2021 - 5:00pm

An opportunity is available for a Research Assistant to join the Structural Biology Division at Australia’s pre-eminent biomedical research institute.

About the position

The role will form part of two newly established labs (Glukhova and Shakeel labs) at WEHI (Structural Biology Division), with common interest in cryoEM. The successful applicant will work 50 % in each of the two labs. Both labs are physically located next to each other. Mission of both the labs is to determine atomic-scale structures of cellular machineries to understand their functions, so that these structures can pave the way to drug discoveries.

The successful applicant will assist in – production of high-quality recombinant proteins in insect and mammalian cells expression system; preparation of protein complexes for structural and biochemical studies; development of innovative biochemical assays to assess protein interactions or protein enzymatic activity; structural studies of protein/ligand complexes (primarily using cryoEM, amongst other structural methods). The applicant will assist with maintenance of insect and mammalian cells in culture.

The ideal candidate will have experience in multiple aspects of protein biochemistry including cloning, recombinant protein expression and purification. Some experience in insect and/or mammalian cell culture, multiprotein complexes/membrane proteins production would be preferred but are not essential.

Key responsibilities:

  • Construct cloning
  • Virus generation, amplification and titration (using FACS and/or Western Blot)
  • Purification of high-quality recombinant proteins from E.coli, insect and mammalian cells and preparation of protein complexes.
  • Assist in the culturing of insect and mammalian cell lines.
  • Monitor and maintain accurate records of stocks and reagents, including constructs, protein stocks and lab consumables, in accordance with relevant protocols.
  • Liaise closely with other lab members to foster team science across the collaboration and enable breakthroughs.
  • Diligently record research findings using Electronic Lab Notebooks in a timely, clear and comprehensive manner

The position will report to the WEHI Lab Heads, Dr Alisa Glukhova and Dr Shabih Shakeel (Structural Biology Division) and work closely with the rest of the team.

Selection Criteria

Essential

  • A relevant Bachelor’s Degree (with Honours / Masters) qualification or equivalent
  • Demonstrated high level skills in molecular biology and biochemistry - including construct design, cloning, recombinant protein expression, purification (using AKTA systems) and characterisation (including SDS-PAGE, Western Blot). In addition to bacterial expression, experience in insect and mammalian cell expression would be an advantage.
  • Demonstrated ability and potential for excellence and productivity in research.
  • Outstanding organisational skills and a high attention to detail.
  • Excellent interpersonal, communication and presentation skills.
  • Ability to execute experiments accurately and with high competence and to analyse, summarise and effectively communicate research data.
  • Demonstrated ability to and to problem-solve within a team environment.

Desirable

  • Experience working with membrane proteins
  • Knowledge of protein structure determination (X-ray crystallography, NMR or cryoEM)

Terms of appointment

This position is available for 1 year in a full-time capacity in the first instance (0.5 FTE split between Glukhova and Shakeel labs), with the possibility of an extension. Salary is HEW5 ($70,971 - $81,520). Up to 17% superannuation and very attractive salary packaging options are available.

General enquiries can be directed to Dr Alisa Glukhova or Dr Shabih Shakeel – glukhova.a@wehi.edu.au or shakeel.s@wehi.edu.au

A position description is available here.

How to apply

Please email your application including cover letter, CV and a letter addressing the key selection criteria in pdf format to jobapplications@wehi.edu.au, quoting reference WEHI/ANSS070421 in the subject line.

Application closing date: 21 April 2021

Note: Applications may begin to be considered as they are received

Institute Life

At the Walter and Eliza Hall Institute we strive to ensure our staff and students enjoy a great working environment. We value diversity and gender equity in our work force and promote flexible working arrangements for staff to balance working requirements and personal needs. We have implemented a number of gender equity initiatives to support women laboratory heads.