Epigenetics is a key regulatory layer determining how the universal blueprint – the genome – is utilized within each cell and during disease.
Students will perform an epigenetic assay, which involves detecting the localization of histone modifications using CUT&Tag (Kaya-Okur, Nat Commun, 2019 doi.org/10.1038/s41467-019-09982-5), and the binding of transcription factors and other DNA binding proteins via the CUT&RUN assay (Skene, eLife, 2017, doi.org/10.7554/eLife.21856).
CUT&Tag can be utilized to determine the localisation of multiple proteins on a single-cell level with engineered enzymes and adapter oligos (Stuart, Nat Biotechnol, 2022, doi.org/10.1038/s41587-022-01588-5 and Bartosovic, Nat Biotechnol, 2022, doi.org/10.1038/s41587-022-01535-4). Additionally, we try to acquire transcriptomic data from the same cell to integrate with single-cell RNA-sequencing results.
Students will learn various methods, including PCR, RTqPCR, cell culture, antibody staining, sequencing library preparation, preprocessing, and analysis of CUT&RUN and CUT&Tag data.
At the WEHI Advanced Genomics Facility, we have a dual focus. Firstly, we collaborate closely with researchers to provide advanced genomics support for their studies, including multi-omic single-cell and spatial genomics technologies. Additionally, we develop cutting-edge technologies in collaboration with biologists to address fundamental questions about basic biology and disease. We also evaluate the viability of new technologies and make them accessible to our colleagues.
As a member of the Research & Development program within the WEHI Advanced Genomics Facility, the student will be part of an exceptional team that includes postdoctoral researchers and other students offering a unique opportunity to collaborate on a wide range of diseases studied at WEHI. Students will learn a diverse range of genomic techniques, experimental design and considerations when applying genomics technologies.