RNA is the functional readout of the genome (DNA). In disease, changes in the sequence and abundance of RNA occur. Sequencing RNA is a powerful means to understand diseases and is used to revolutionise how we diagnose and treat them. As such, petabytes of RNA sequencing data have been generated and are available to the research community. However, current analysis approaches focus largely on RNA’s abundance (gene-expression), to the exclusion of its sequence (transcripts).
Despite this, numerous unique transcripts are expressed, including ones that drive disease. This project will analyse transcripts from large public databases to reveal the diversity of RNA across healthy and disease tissue, with a focus on cancer to aid the discovery of novel biomarkers and/or predictors of drug response.