Professor Philippe Collas - University of Oslo

Professor Philippe Collas - University of Oslo

Location: 
Seminar Room 2
Start Time: 
Fri, 15/02/2019 - 10:00am
End Time: 
Fri, 15/02/2019 - 11:00am

​Reorganization of spatial genome organization during differentiation

​Special seminar hosted by Dr Rhys Allan

Reversible changes in spatial genome conformation establish blueprints of developmental gene expression. These changes reflect a 4-dimensional orchestration of temporal gene regulation. We will discuss evidence for large-scale alterations in genome conformation during dual lineage adipose stem cell differentiation. These change entail long-range associations between topologically associated domains (TADs) forming repressive TAD cliques. Computational 3D genome modeling using a new platform developed in our laboratory (Chrom3D), FISH and analyses of single-cell Hi-C data support a concept of long-range TAD-TAD associations. Our data further suggest that TAD cliques may stabilize heterochromatin at the nuclear periphery. Lastly, we provide evidence of dynamic TAD cliques during mouse embryonic stem cell neuronal differentiation and during mouse B cell reprogramming towards pluripotency. TAD cliques therefore seem to constitute a new evolutionarily conserved level of higher-order chromatin organization that may strengthen the robustness of developmental gene silencing.

Paulsen et al. 2017. Chrom3D: three-dimensional genome modeling from Hi-C and nuclear lamin-genome contacts. Genome Biol. 18, 21-29

Paulsen et al. 2018. Computational 3D genome modeling using Chrom3D. Nature Protoc 13, 1137-1152