Miss Dawn Lin - Immunology division

Miss Dawn Lin - Immunology division

Location: 
Davis Auditorium
Start Time: 
Wed, 17/04/2019 - 1:00pm
End Time: 
Wed, 17/04/2019 - 2:00pm

Clonal trajectories of dendritic cells during steady-state and emergency development

​Wednesday seminar (this is a PhD completion seminar)

Dendritic cells (DCs) represent a unique lineage that play a critical role in immunity against bacteria, viruses and cancer. DCs, like most blood cells, derive from haematopoietic stem and progenitor cells (HSPCs) in a process called haematopoiesis. While the steps in DC development are well characterised, the kinetics and diversity of cell production by individual clones (i.e. clonal trajectories) and how these aggregate to a population level is not clear. The major focus of Dawn’s PhD was to investigate this diversity and the changes that occur during ‘emergency’ conditions, where DC numbers rapidly increase.

First, using a single cell tracking technology called cellular barcoding, Dawn developed an experimental and computational framework to visualize and characterize the clonal dynamics of DC development. She identified multiple classes of clonal trajectories and demonstrated that these clonal behaviours are programmed within single HSPCs. Second, through integrated analysis of single cell division, lineage tracing and gene expression with single cell RNA-sequencing, Dawn investigated the clonal dynamics during emergency DC generation in vivo. This work revealed complex molecular controls regarding cell proliferation and differentiation within individual HSPCs that account for the increase in DC numbers.

These findings enhance our understanding of the clonal level control of stem and progenitor cell fate, with implications for maintenance or manipulation of DC numbers in health and disease.