Followed by Genomix mixer in the Tapestry Area, with light refreshments provided by IDT
Uyen Nguyen – NGS Field Application Scientist, IDT Technologies
NGS Assays for Challenging RNA Samples
Library preparation is a necessary step to turn input DNA or RNA into final libraries before targeted capture or sequencing. Converting samples into NGS libraries is often challenging due to the low quantity and quality of RNA in such samples. Here, we present a workflow of our xGen Broad-range RNA library prep kit and xGen hybridization capture for targeted next-generation sequencing (NGS).
Nick MacDonald – Deakin University)
Tasmanian devil immune gene expression in response to transmissible cancers
Populations of the Tasmanian devil (Sarcophilus harrisii) are declining due to the transmissible cancer known as Devil Facial Tumour Disease (DFTD). DFTD encompasses two clonal cell lineages of cancer that spread from one individual to another via allograft. This disease exhibits an alarmingly high mortality rate, with infected devils often showing no discernible signs of an immune response. My project aimed to characterize immune gene expression to understand how the host responds to DFTD and identify the cause of the lack of response. I used custom panel gene expression approach, utilizing the IDT Hybridization capture protocol and identified genes showing differential expression in DFTD infected hosts.