-

Professor Eduardo Eyras – Australian National University

18/07/2023 11:00 am - 18/07/2023 12:00 pm
Location
Davis Auditorium

WEHI Special Seminar hosted by Drs Ramyar Molania & Alex Yan
 

Professor Eduardo Eyras

EMBL Australia Group Leader, John Curtin School of Medical Research, Australian National University

 

Characterising the (epi)transcriptome at single-molecule resolution with long-read sequencing

 

Davis Auditorium

Join via ZOOM

Including Q&A session

 

We describe our recent developments in the study of transcriptomes and epitranscriptomes using long-read sequencing. The epitranscriptome embodies many largely unexplored functions of RNA. A major roadblock in epitranscriptomics is the lack of transcriptome-wide methods that detect multiple RNA modifications, identify RNA modifications in individual molecules, and estimate modification stoichiometry accurately. We addressed these issues with CHEUI, a new method that processes signals from nanopore direct RNA sequencing to identify RNA modifications at single molecule resolution from any sample. CHEUI outperforms other methods in detecting m6A and m5C sites and quantifying their stoichiometry, and further reveals a non-random co-occurrence of m6A and m5C in mRNA transcripts in cell lines and tissues. We also describe RISER, a method to perform in-silico biochemical-free enrichment or depletion of RNA classes in real time during direct RNA sequencing. RISER identifies RNA classes directly from the first few seconds of the signal without needing basecalling or a reference and communicates with the sequencing hardware in real-time to enact biochemical-free targeted RNA sequencing. We illustrate RISER for the enrichment and depletion of coding and non-coding RNA, demonstrating a 3.4-3.6x enrichment and 6.2-6.7x depletion of non-coding RNA in live sequencing experiments. RISER and CHEUI unlock novel ways to study transcriptomes and epitranscriptomes and enable discoveries and new applications across multiple fields.

 

Eduardo Eyras is an EMBL Australia Group Leader and Professor at the Australian National University (ANU), where he develops computational methods to study transcriptome and epitranscriptomes and their alterations in cancer using long-read sequencing technologies. Before joining ANU, Eduardo Eyras worked at the Sanger Institute (2001-2004) and was group leader at the Pompeu Fabra University in Barcelona, Spain (2005-2019). During this time, he developed methods to annotate RNA alternative splicing in genomes, contributed to the landmark analyses of the human, mouse, rat, chicken, and cow genomes, and led a research program on Machine Learning applied to RNA biology and cancer.

All welcome!

Support us

Together we can create a brighter future

Your support will help WEHI’s researchers make discoveries and find treatments to ensure healthier, longer lives for you and your loved ones.

Sign up to our quarterly newsletter Illuminate

Find out about recent discoveries, community supporters and more.

Illuminate Summer 2023
View the current issue