Transcription rates are coordinated with cell size to maintain constant concentrations of mRNA in differently sized cells. I will describe how we are using quantitative measurement of mRNA and protein abundance at the single-cell level together with perturbation studies and mathematical modelling to understand the mechanistic basis of this phenomenon. I will also describe recently developed experimental and computational approaches we use to visualise and quantify many different proteins within the same single cells, at high resolution. These versatile approaches have the potential to be widely applied in perturbation screening studies and will enable systematic mapping of multiscale atlases of biological organisation.
Scott has a background in Theoretical Physics and Molecular Biology. He studied a PhD at the John Innes Centre in Norwich, UK, on mechanisms of epigenetic memory in plants, before moving to the University of Zurich in Switzerland as an HFSP and EMBO postdoctoral fellow. In Zurich, Scott worked on mechanisms of mRNA concentration homeostasis in mammalian cells. In 2021, Scott started his group at Single Molecule Science, the EMBL Australia node at the University of New South Wales in Sydney. His group works on quantitative regulation of gene expression at the single-cell level, primarily employing microscopy and systems biology approaches, and mathematical modelling.