WEHI Technical Seminar hosted by Perkin Elmer
 

Antony Sheehan
Director of Immunoassays – Site Head / TGR BioSciences Pty Ltd

Protacs and Alphalisa Surefire Ultra technology for high throughput screening of kinases in drugy discovery

Davis Auditorium

Join via SLIDO enter code #WEHIseminar

Including Q&A session

Screening of compound libraries for modulators of cellular function can require high throughput technologies amenable to automation. Protein phosphorylation within different cellular pathways is a common event  for measurement of drug effectiveness. The AlphaLISA SureFire Ultra  panel of protein phosphorylation kits provide such rapid, homogeneous assays, allowing drug effectiveness to be analysed for large or small  sample numbers. More recently there has been an increased focus on ‘Total’ SureFire Ultra assay development and its combination with PROTACS for targeted protein degradation analysis. For such work, ideally, we require assays against the ’Total’ proteins for directly assessing target engagement as well as assays that measure the downstream phosphorylation effects resulting from targetengagement.

Data will be presented on numerous recently developed assays and  PROTACS examples, highlighting the utility of Alpha SureFire technology as a platform of choice for both high and low throughput screening  applications in drug discovery.

All welcome!