Dr Andrew Keniry - Epigenetics and Development division

Dr Andrew Keniry - Epigenetics and Development division

Location: 
Davis Auditorium
Start Time: 
Wed, 03/04/2019 - 1:00pm
End Time: 
Wed, 03/04/2019 - 2:00pm
 

Improved understanding of female embryonic stem cells reveals unexpected mechanisms of epigenetic silencing

 

Wednesday seminar​

Female mouse embryonic stem cells (mESCs) are significantly harder to propagate than their male counterparts. This has resulted in the majority of mESC studies being performed in male cells, and therefore our knowledge of female pluripotency is lacking. We have developed a reporter line of female mESCs and have used this to develop robust culture protocols. We have experimentally proven the utility of these cells for the study of pluripotency, differentiation, induced pluripotency and X inactivation. Using these cells, we have performed screens for epigenetic regulators of X inactivation, revealing unexpected mechanisms are required for epigenetic silencing of the X chromosome, including nucleosome positioning and circadian rhythm. These experiments highlight the complex interplay between differentiation processes that are particular to female pluripotency.