Dr Alisa Glukhova, Structural Biology division

Dr Alisa Glukhova, Structural Biology division

Location: 
Online
Start Time: 
Mon 22/03/2021 - 11:00 AM
End Time: 
Mon 22/03/2021 - 11:30 AM

WEHI Postgraduate Lecture series hosted by Dr Brad Sleebs

Dr Alisa Glukhova
Laboratory Head, Structural Biology division

Single particle electron microscopy
 

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Includes Q&A session

 

Single-particle electron microscopy is a technique that allows to determine the structures of proteins embedded in vitreous ice. Recent developments in data acquisition and data processing led to achieving near-atomic resolution for well-behaving protein samples. However, the unique ability of single particle analysis to classify particles based on their similarities and to focus the refinement on local structural features of interest makes it possible to determine high-resolution structures of difficult, flexible and heterogeneous samples. In this seminar, I will introduce the basics of cryo-em single-particle analysis using the structure of SARS-CoV-2 Spike protein in complex with neutralising nanobodies.

Dr Alisa Glukhova is interested in understanding the molecular details of different steps in Wnt pathways. Wnt signalling is initiated by Wnts that bind to their receptors and co-receptors, leading to different cellular responses. Multiple steps of the Wnt pathways have been explored as therapeutic targets for cancer treatment, however, due to the extreme complexity and importance of Wnt signalling for normal tissue homeostasis, its safe and selective targeting remains a challenge.

Alisa and her laboratory are combining cryo-electron microscopy and x-ray crystallography with biochemical, biophysical, and pharmacological assays to gain insights into the atomic-level details of individual proteins as well as large complexes involved in Wnt signal transduction.