Long-read sequencing for transcriptome and epigenome analysis

Long-read sequencing for transcriptome and epigenome analysis

Project details

 

Long-read sequencing as generated by Pacific Biosciences’ new Sequel platform or Oxford Nanopore Technology’s MinION and PromethION devices provide researchers with the ideal tool for resolving transcript architecture, studying methylation patterns genome-wide and assembling genomes. 

This project will explore a number of applications of long-read technology using data sets that look at splicing patterns in platelets, methylation changes associated with X-inactivation and genome structure in cell-lines. It will involve collaboration with researchers at CSL and the successful candidate may be eligible for an industry top-up scholarship.

We are looking for a student with a strong statistical or computational background (e.g. an undergraduate degree in Statistics and Mathematics), programming skills and an interest in biology.

About our research group

The Ritchie and Blewitt labs collaborate on the development of analysis methods tailored to new applications of genomic technology in biomedical research that includes:

  • Statistical methods for RNA-seq data analysis.
  • Analysis of high-throughput sequencing data from genetic screens.
  • Open-source software for genomic analysis distributed freely to researchers worldwide via the Bioconductor project.

The Ritchie lab is a member of the MinION Analysis and Reference Consortium and has been successfully generating data on the MinION platform for the past 12 months. This project aims to build on this experience and explore emerging applications of this technology and PacBio sequencing, which is also available at the Institute.

Researchers:

Dr Matthew Ritchie

Dr Matthew Ritchie profile photo
Dr
Matthew
Ritchie
Laboratory Head
Dr Charity Law working on a computer
Dr
Charity
Law
Molecular Medicine division

Project Type: