The Walter and Eliza Hall Institute of Medical Research

Ms Lisa Connolly

Details

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Division: Systems Biology and Personalised Medicine

Research Overview

Before joining the WEHI Proteomics Laboratory as a data analyst, I worked over 14 years in the Joint Proteomics Facility (JPSF) where I contributed to many collaborations between scientists at the Ludwig Institute or Walter and Eliza Hall Institute.

My area of expertise is the analysis of mass spectrometric data, and as a research technician I am involved in the fee-for-service component of the WEHI Proteomics Lab. My role is to use algorithms such as Mascot to analyse mass spec data and send out QC reports and Scaffold results files.

I can also offer a more detailed analysis to validate the data within Scaffold, perform extended expert analyses which involve de novo interpretation of MS/MS data or manual trawling where we attempt to identify and extract as much relevant information from the raw mass spec data as possible.

Major Publications

1. Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000 Jul 7;102(1):43-53. PMID: 10929712
2. Moritz RL, Hall NE, Connolly LM, Simpson RJ. Determination of the disulfide structure and N-glycosylation sites of the extracellular domain of the human signal transducer gp130. J Biol Chem. 2001 Mar 16;276(11):8244-53. PMID: 11098061
3. Verhagen AM, Silke J, Ekert PG, Pakusch M, Kaufmann H, Connolly LM, Day CL, Tikoo A, Burke R, Wrobel C, Moritz RL, Simpson RJ, Vaux DL. HtrA2 promotes cell death through its serine protease activity and its ability to antagonize inhibitor of apoptosis proteins. J Biol Chem. 2002 Jan 4;277(1):445-54. PMID: 11604410
4. Kapp EA, Schütz F, Connolly LM, Chakel JA, Meza JE, Miller CA, Fenyo D, Eng JK, Adkins JN, Omenn GS, Simpson RJ. An evaluation, comparison, and accurate benchmarking of several publicly available MS/MS search algorithms: sensitivity and specificity analysis. Proteomics. 2005 Aug;5(13):3475-90. PMID: 16047398
5. Debrincat MA, Zhang JG, Willson TA, Silke J, Connolly LM, Simpson RJ, Alexander WS, Nicola NA, Kile BT, Hilton DJ. Ankyrin repeat and suppressors of cytokine signaling box protein asb-9 targets creatine kinase B for degradation. J Biol Chem. 2007 Feb 16;282(7):4728-37. PMID: 17148442

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