';Aggcctcaacaaggaccgag@Taattgtatccgtcattcat@;2710Acctgtttat@Agcaaggtaaaactccgctg@xAgtttttataattggattga@zAttattatcatcatatatgt@Ggagaagaat@Tattaaattt@Tgtatttattcctgcagcaa@ Ä#sOGeneQS@FJ? @ @y@f) @09L!h@r@V(@?D<B/ 0`B8@1C2E3G4I5K6M7B9FHBAM2001MAY84@w Working WEHI STSs:*0002f12--0055:12--0055:12-000412-000512-000612-000712-0008 12-0009 12-0010 12-0011 12-0012 12-001312-001412-001512-001612-001712-001812-001912-002012-002112-002212-002312-002512-002612-002712-002812-002912-0030 12-0031!12-0032"12-0033#12-0034$12-0035%12-0036'12-0037(12-0038)12-0039*12-0040+12-0041,12-0042-JGnJ(x(t US Letter A4 LetterUS LegalInternational FanfoldComputer PaperEnvelope (#10)els^23456789 123456789 123456789 123456789 123456789 123456789 123456789 123456789 123456789 12345 A BsMain Dictionary G< L15M,,./: AM PMX{ 2/25/97@ ALTERNATIVE NAMESAPPROXIMATE LOCATIONCHROMOSOME ASSIGNMENT COLUMNCOMMENTS DATE OF ENTRY DATE OF MODIFICATION FRAGMENT LOCATION GENBANK IDISOLATE LAB YAC NAME LOCUSPCR PRODUCT SIZEPRIMER AF ENTRY DATE OF MODIFICATION FRAGMENT LOCATION GENBANK IDISOLATE LAB YAC NAME LOCUSPCR PRODUCT SIZEPRIMER A PRIMER BPROBE DEFINITION PROBE NAMEPROBE SEQUENCE PROBE SIZE PROVISIONALPUBLICREACTION CONDITIONSROW SERIAL NUMBERSTATUSSTS WELL LOCATIONYAC INSERT SIZE YAC LIBRARY YAC NUMBER YAC POOL                D ASTSBG APrimer aBAPrimer bBALocusBAAlternative namesBAPCR Product SizeBA Probe NameBAProbe SequenceB A Yac NumberBG r  AChromosome AssignmentB ACommentsB A Date of EntryBG  ADate of ModificationBG A Serial NumberBG L120 A Well LocationBAYac PoolBG  NAFragment LocationBA Yac LibraryBG   A23D7 MunI Tony Triglia size selected 3D7 R1 FCR3 A42AReaction ConditionsBG  A941.00', 55 1.00', 72 1.00', 35 cycles 941.00', 45 1.00', 72 1.00', 35 cycles 94 1.00, 50 1.00, 72 1.00, 35 cycles Other- see comments AProbe DefinitionBG     A7Anonymous marker Known gene sequence cDNA clone YAC endh7AIsolateBG   A B8 3D7 FCR3S A Probe sizeBAStatusBG  A'Mapped Unmapped Sequenced Non STS Probe'APublicBG  AYes NohA ProvisionalBG  AProvisional FinalhAApproximate LocationBG  ALeft Middle RighthAYac Insert sizeBG r A Genbank IDBARow  BG r AColumnBG r@  A Lab Yac NameBG r @@@XARecord@@AControl Screen@@AChromosome Listing@@A STS Listing@@A Probe Listing@@ANotes@@A Record CopyARecordB @"A     B     C     D       E     F@    A$,.#YesfNoG     H   O  I@    A$,.#YesfNoJ@    A$,.#YesfNoK   O  M     N*    O*      P@    A$,.#YesfNoQ     R     T     U     V     W@    A$,.#YesfNoX     Y      Z      ]       ^@    A$,.#YesfNo_     `     a@    A$,.#YesfNob     A&P $ʸ79E6P9G7ʸH9V6PIW7ʸ!(/%P#1'ʸ!*/P   B%ʸ8FP:5Hʸb'P(Jʸ+`P+ QXʸzJP IkyDC ʸN sP I O ʸ`P B ʸzq ,,ksy ,Z`l+M3   DYl(+ 3l+3 h,,s ,Z   V gE   pC  p  pN  p, p!pp p  F p    zE,,kyD,ZY}gz+Ygs3)',,()G,ZzDr,,kHyo,Z'|I,(,Z,,[,Z F, ,    H``zIIkJy ^\!f:z ky"f:z!II`ky#f:z"ky$f:9GP%m@%C   ESTSC   EPrimer aC   EPrimer bC   ELocusC     EAlternative namesC   EPCR Product SizeC   E Probe NameC   EProbe or STS Sequence C   E Yac Number C   E Chromosome C   EComments C   EYac PoolC   E Date of EntryC   EModification DateC   E Serial NumberC   EFragment LocationC   EMenuC   E Yac Library  C   EReaction ConditionsC   E DefinitionC   EIsolateC   ESizeC   EStatusC   EPublic C   E Insert size!C   E Genbank ID"C   ERow#C   EColumn$C   E Lab Yac Name%C   Ebp @B) Bv)    A  !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIBw)        /13579;=>?ACE GABCDEFGHIJKM NOPQR T U  V WXYZ]^_` ab"""A"""""  "1 "/ "5 " "C"E""?"G""">" "7"3"""""9"=";"/ "5!"""C#"E$"%"?&"G'"/("5)"*"C+"E,"-"?."G  /"/0"51"2"C3"E4"5"?6"G7"/8"59":"C;"E<"="?>"G?"/@"5A"B"CC"ED"E"?F"GG"/H"5I"J"CK"EL"M"?N"GO"/P"5Q"R"CS"ET"U"?V"GAControl ScreenB @  A '`<i `'a<[Qc?XR`Kzi `<i`'*sLc `'dQBfRc} c`'LY6yV p8xA 7BnAyV BY6yVhY6yV   C}L@QL f L f>PAf ; LL %g 6Dc `'6UQ9RREc 6c`'} `'QR  E `'@ `'2Q/R"@ @`'IFW  ֐Ww nv 7Blw BWwhWw }PW ֐-M   Gl 0M8-J85F-M X@C E-Wellcome Foundation Malaria Sequence DatabaseC  E Add an EntryC  EEdit an Existing EntryC  EExport Data to Excel C  EData entry and Editing C  ESearch for an Entry C  ERecord Manipulation C  E View Data   C  EChromosome OrderC  ESTSC  E Probe NameC  ESend an update to RossC  EHELP @BP˲BP˲Ar  !"#$%&'()*+,-./012345678rAChromosome ListingB  @A@    A$,.#YesfNo  #B     D     A/=[, ,? Pv33,?wPl+0q>3,?PF+0>3,' # V"g  B' 'pC%&p &pN$%p,&p!pp&pCT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenu A* UU  UU* @B>3333@A$ $B>3333@ABQ3333@ A STS ListingB @A@    A$,.#YesfNoB     D       A0>!, ,?PB33,?Pjl+0>3?lP+0f>3,' # V"gB' 'pC%&p &pN$%p,&p!pp&p  CT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenuA* UU  UU* @B>3333@A$ $B>3333@ABQ3333@  ! A Probe ListingB @A@    A$,.#YesfNoB     D     A0>, ,?P@33,?qPl+0p>3?Pp+0>H3' # V"g  " B' 'pC%&p &pN$%p,&p!pp&pCT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenu !#*A* UU  UU* @B>3333@A$ $B>3333@ABQ3333@ ANotesB  "$ AP  Vg pCp pNp,Bp!pppp  @C   #% ASTS- Nomenclature is of the form X00-0000 X is single letter lab designation I- Institute of Molecular Medicine W-WEHI F-Florida M-Menzies R- NIMR S- Sloan Kettering First two numbers are chromosome number UsBe 00 if not assigned to a chromosome Next four digits is STS number Nomenclature for corresponding primers X00-0000y where y is either a or b corresponding to primers in the listC   EMenu @B[d^ $&  B[d^A   B[d^A Record CopyB @"A     D     I@    A$,.#YesfNoM     R     V     ^@    A$,.#YesfNo %' e`      a@    A$,.#YesfNob      A&P $ʸP)NP I(H ʸ)u ,,w( ,Z)I,, (H &( B,Z)Hv,,L(s,Z)MIN( ) ("f:)!I(#f:)C$"($f:m@%C   ESTS C   E Yac Number C   EYac PoolC   E Yac LibraryC   EIsolate C   E Insert size"C    ') "ERow#C   EColumn$C   E Lab Yac Name @B) B) A& &B)      ADIM R V ^` ab                    (R            ! " # $ % & ' ( ) * + , - . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I AW 3-00081/03/96 2A>    >TAnonymous markerU3D7V300 bp[Middle^F_8s' A gA gA gA gA gA gA  g  g g g g g g g g  g  g  g  g  g g g g g g g g g'C   g g g g g g  g     @ Find New entryTestFind all/Control ScreenChromosome sort STS Sort Probe SortUpdate for Ross AFind. BHH +6G{HH d'@E7 Y*``QA New entryE7 (QATestEQAFind all/Control ScreenBHH%.7@G{HH%.d'@E7-/ { (*`QlAChromosome sortEL157'` (> e ? QlASTS SortEL157 '`>  ?QA Probe SortEL157 '`>  ?Q AUpdate for Ross.0  BHH +6G{HH d'@EI7 Hzr*`@ $@@XYes                    />               d g g g  gQBUUXcM  A$,.#YesfNoA : AM PM@ HelveticaGenevaCourierTimesArial@`823KAPICT *A *  $HHff33̙ff33Bff33ffffffffffff33ff33333333ff333333ff3313 Cff33̙ff33̙̙̙̙ff̙33̙ffffffffffff33ff33333333ffD333333ff33ff33̙ff33ff3324 Eҙffffffffffff33ff33333333ff333333ff33ffffffffffff33ffffffffF̙ffffff33ffffffffffffff33ffffffffffffffffffffffff33ffffff33ff33ff33ff33ffff3333ff33ffffffffffff35 G33ff33333333ff333333333333̙33ff33333333333333ff33333333ff33ff33ff33ffff33ff3333ff3333H333333333333ff333333333333333333ff333333ff33̙ff3346 Iff33ffffffffffff33ff33333333ff333333ff33wwUUJDD""wwUUDD""wwUUDD""57 KһwwwwwwUUUUUUDDDDDD""""""   L            69 YMR  3 *A`PICTAFF FF FF"% q% ?0% 4?1 "0 #### ###  >1 `7: BFF "+ "D">dMDPLk UUUUq.1D1>D DD=851dMDPL  C"1 ############### UUUU  FF # #######"2  "1#9= D FF#"* dMDPL}/ wwwwq> :3&* . 0539:88 3%&V """"q> :3&* . 0539:E88 3%&dMDPL  "&####################### """"  Y12-0001@12-0043@12-0091@HW@[Acagtttggaaacataaata@ gaagacaa@Ggtcatggtgatgataacaa@Ttattcaagcgatgtaggaa@ Ttatttaaatcttcaagtaa@kAagattagggtattatggtt@Agtaccttctacactctcct@Catgatgactgctatctacc@Taattgtatccgtcattcat@Tatactttccatgcttctct@Tccctatagatgattcttgt@_TctttttcttcttcctctatTttttccatgttatttattg@Ã"@Ã4P3a9f@4b4fFX6a9f@Aatgcatcatttcataatat@Agtttttataattggattga? CD:F=A@AACBECGDIEKF:@ FFF ############"%  "&# FF"&")" ,"@  q<D <G@ D< "<#####  FF ###"1"% "? 3A(PICT08(ځ`V< K(@>`B8@1C2E3G4I5K6M7B9=A -A09 ~8  H I@B%/<@p@? R Q Q B&PP`p`3A3KPICT @B A   HH"Lff33̙ ff 33 Bҙ ff33ffffffffffff33ff33333333ff333333 !ff"33#$%&AC Cҙ'ff(33)*+,̙-ff.33/0̙1̙2̙3̙ff4̙335̙6ff7ff8ff9ffff:ff33;ff<33=33>33?33ff@3333DA33BCDEffF33GHIJKffL33MNOP̙QffR33STUVWffX33YZff[BD Eҙff\ff]ffff^ff33_ff`33a33b33c33ffd3333e33fghiffj33klffmffnffoffffpff33qffrffsfftff̙uffFffvff33wffxffyffzff{ffff|ff33}ff~ffffffffffffffffffffff33ffffff33ff33ff33ff33ffff3333ff33ffffffffffff33ffCE G33333333ff333333333333̙33ff33333333333333ff33333333ff33ff33ff33ffff33ff3333ff33333333H33333333ff333333333333333333ff333333ff33̙ff33ffDF I33ffffffffffff33ff33333333ff333333ff33ٻڪۈwwUUDDJ""wwUUDD""wwUUDD""E KҪwwwwwwUUUUUUDDDDDD""""""   L   3 NProvisionalomInOrderedOverlapPrimersSequencedSequences()*+,-./012356 Cs :User Dictionary!&@D2F6F&dehlopÁ@fgijkmnqrstuvwÂ#  !"#$%'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bcă#`a017W12-0018W12-0019W12-0020W12-0021W12-0022W12-0023W12-0025W12-0026W12-0027W12-0028W12-0029W12-0030 W12-0031!W12-0032"2f?tMA44oC annealing@A>    >TAnonymous markerU3D73D73D73D7B8V296bpWcompleteXYesYFinal[Left\75606560190^GBBAE_85575A W 12-0004BAAATAAACTCGTTTTTCTCATTACAAACGTATATTCAAGTAAAGTCG F107G8G3FI341 313 717J12L21/03/96M27/03/96N4P448R3D7 MunI3D7 MunI3D7 MunIS43oC annealingTAnonymous markerU3D73D73D7D7 MunIFEPro 2.0F!\@\@C2(H Pro 2.0 - 2.1I-w @‚69:;<=>^_‚G-‚QMNOP‚Sfg‚z-./0a‚‚/01‚ Ã"PfÃ# 34567Ã#PÃ$stÃ&PkÃ&p]^Ã(fÃ) JKLMNOPQRSÃ)`jÃ10HIÃ4Ã4Ã5@-Ã6PjÃ6Ã7pIÃ8P?@Ã:@789:;<=Ã:PmÃAkÃA ÃA`567ÃB0VWÃB@ ÃCkÃC\]^ÃDpSTUVÃZ`%'(Ãa 'Ãd !"#Ãe./012Ãi0+,-.ÃiBCDEÃjP$%Ãq@01ÃrPCDEF0\A W 12-0001BATATCGTAGAATGTTTGCTTGAA CTGTGGTGGTGGTTTTGGTAGTTG D2F6FF196G2F6FATTGTACTGTCGCAGGAGCTCAGAAAATGGAGTTACTAACAAAAAGGATATCGTAGAATGTTTGCTTGAAAATCTTAAAACAAAAGCAAAAACTTGTCCAAACCAAGTTAATGGCGAAAACCAAACGTGTGATTCCCTCCCTCATGTTGAAGACGATGACGATGAGGAACCCCTTGAAGAAACAGAAGAAAATACTGTGGAACAACCGAACBoATTTGTCCGCAACTACCAAAACCACCACCACAACCAGGGGATGAAGACGGTTGTAAACAGGCATCACCAGCACCTAGTGAAGGCACAGAGAACCAACCCCCTGTGATCAAGA@A I349 1262J12Homology with var-31ITWcompleteXYesY Provisional[Left\19013565^ECD_519A W 12-0005BTGGAGAAAAGAAATTAGGAAATA CGATCCAATATACCATCAGCACTT F104G4B4FAATGTAATAATCATGGAGAAAAGAAATTAGGAAATATATTAGAAGTATCTCATGATAATGTATCGAATGAATTGCCGAGAATGATATCTGAAAAAAGTGCTGATGGTATATTGGATCAACTGTTTGATGAACAATCCCTAAAGACATCAAATACCTTAAGTAGTGATATAATGGGAGAAGTAAATGAT @A I341 717J12L21/03/96M27/03/96N5P48R3D7 MunI3D7 MunIA45oC annealing5X Ä#sÄ#w0Ä# Ä#()*Ä#*Ä$1_Ä$XÄ$?@AÄ$KLMÄ%STÄ%#fgiÄ!mrs @fgijkmnqrstuvw‚#  !"#$%'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bc8BeBeenBelieveButByCdna)ChainChromosomeClone)ComplementConfusionControlDecemberQ Y Ä#s ‚# 1i1995AndKChromosomenDecember]F@%In3d7@Mun1Reverse@vSexualCX625400006@2X766430001@*Yac@p ‚U@d123_3d7BMuniSize45ocX?ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_fgjknqruvwSequence$%6@bcimtYacs3d7  !"#$%'()*+-./0123456789:;<=?@ABCDEHIJKLMNOPQRSTUVWYZ[\]^_`abcfgijkmnq +,-.9:;OS +Gene3d7sB8|ÃC @RPRIMER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALPIAMH@* A+ ,B-. B/ 0(3qÃC [ÃCPYÃC`ÃC ÃD?VÃD ÃD=ÃE ^ÃEP.ÃF>ÃFp*ÃG(ÃG,ÃH0XÃH!ÃI@nÃI`ÃJP ÃQ09ÃQ`CÃRNÃR@ÃR`5ÃSGÃUP8ÃV#ÃY Complete  !"#$%'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRST !"#$%'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRSTQJCompleteU3YesMiddlepÃ!N"Ã(@ER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALK@x^At@IWM@TBUBWTj Ji AZMU @A>    >TAnonymous markerU3D73D7V187bpWcompleteXYesYFinal[Left\19065^ED_59A W 12-0006BTTTCCTTTATCACCAAACCTGTT CGACTGTGTCTTTTTCATCTGAGG F228G1E9FAACAATCTTTTTTATAACATCATTATACTTGTATCTCTCAATGGTCATCTTCTTTCCTTTATCACCAAACCTGTTTTCACCATTTTTTACATTTTGTTCAACATCCACGTTTTCGTTTTTTTCTTCATACATATCATCAACTCCTATTTCATCTAACAATTTCTTCATATCCATTTTAACAAAACCTGTGGGCTTTCTTGTCCTTACAGGTD7Y Provisional[Left\190nITWBCTTGGCATATACAATGAAGCAGGTTGCTCAATACATATATGCTCTTCCTCAGATGAAAAAGACACAGTCCTTTTTACTACGTCACTATCTATATTCTTCCTTTTAAAAGTTACCTGTTTCTTCTTTTCATCATCATTCACATCCTT  e@A  I341 313J12L21/03/96M27/03/96N6P44R3D7 MunI3D7 MunIA49oC annealing@A>    >TAnonymous markerU3D73D7V356 bpWcompleteXYesYFinal[Left\190135^EC_51A W 12-0007ft\190135?sH @& @sA+ ', `B-. B/ 0(. B/ 0((((0(((0((((((UBATATTACAAGGTTTAGGAGGTATCTCATTATTATTCGTTGAAGGTATF176G2E4RATTTTATTTATTATTTTATATTACAAGGTTTAGGAGGTATATTCCTTGGGTTTTTTTCAGATATATATGGAAGAAAAATATGTTTAAATATATCCTTTTTTATTTTATTAATAATATTTATTCTTTCTTTTATTACGGTGAAAACATCTTTCTTTTTGTTTTATCAAAACATACCTTCAACGAATAATAATGAGAAGGGCCTGGATTTATTB.CTTTTATACTAATACACCTCATACACAAATAAATGCATATCATGAA@A I341 313 95J12L21/03/96M14/04/96N7P441R3D7 MunI3D7 MunITony Triglia size selectedS43oC annealing 4b4f4f6rP4h7rF5'k1cl12b55k5a11f 5b11fV5b12fJ5b1fS5c11f35c12f5d9f5e11f5e8f^5f11fB5f12f"5h11fK5h12f-5h1fX60@6a2f?6a4f>7e12r:8a11r8b10f,8d7f8e6f8e7f 8g2r48g3f8h4f0Abra6Atp2%Atp4Clat$OraAaaaaagaatattgaaagta7Aaaagtttataaaaatagat Aaaatacgaccacacaaatg4AaagaaattgaaaaaataatAaagagttaaattagttataAaatgtaaacacattaggaa Aatatctttataacattttg"Aatgaaaaatatgatgaagaac I1271 492J12L21/03/96M27/03/96N13P146R3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D7V282WcompleteXYesYFinal[Left\60225^BA_1112A W 12-0013BATATTCATACAATTTCTGAGCCAGTATGTAGAAACTGTGTACTG F800G1H11I1271 946 492J12L21/03/96M27/03/96N13P14106R3D7 MunI3D7 MunI3D7 MunIS50oC annealingTAnonymous markerU3D73D73D792h`  A>    >TAnonymous markerU3D7B8V262 bpWcompleteXYesYFinal[Left\135120^CH_111 A W 12-0010BACTGCGCTTAAATAATAATCACAA CTCAAATAATCTATACTACCGCTGTTF230G5A11FAAAATGTAAACACATTAGGAAGGGAAAATAACCCTTCTCTATTCTCATCAAGAAATGAACAACTGCGCTTAAATAATAATCACAACATCATGAATAATAACAACACCAACAACAATAATAATAATAACAACAATAATAATAATAACAACAATAATAATAATAATAACAATAATAATGGAAATAATAATAACCATTGTAGTCATGGAGATACI341 313J12 AvAaafijAaaatacttattaggttcctZAaaattaaataaccgtggac;Aaactaacaacgaaaatgct2AaataaactcgtttttctcaAaataaatgttggtatggtgHAaatatgaatatggatactaVAaatcaaatattgtttcaaa!AaattagttatagagaaaagAaatttaaatattgaagaagAacvAagkuAagcgactcgttgtgtaaaa>AatgAatataatagagaacatctt_Aatatcagtgggaaaatgtg=AatattttctgatatttcatXAattataggacacttcgttcAattcttgtgcttcatctaaRAcarvAcaattagtggaaataaaacGAgcaaggtaaaactccgctg?@JKLMNOPQRS^_0CDEFGHIJK[`fgijn@%'(-01?@AXYZ[P !"#$%MNOPku` )*56789:;<=\]^_jmqp !"#$%'()*+,-.0KLMTUVafgjmnrs !"#$'(/01345./0123456IJKLSTUVfgi"#$'-./0STYZaijn‚! !"#()*+./0?@HIkst`g CCGGTATAATTAATAAATGAAATAF248G6D11FAAAAAGTTTATAAAAATAGATGTGATGGTGTATGTGTATTAAATGATAATATATATATTTGGGATAAAATAAAAAAATGTATATATTTGTATACACCATATAATAATGTATGGTATATCTTTTTAAATATATATGAAGAAATGTCTCTAAGAAAAAATACACTTTGGTCTTTATGTAGTAGTGATATTAAAAATGATATTAATGTAAATATBvAAATAAATCATATTATAAATATCATAATAATATTTCATTTATTAATTATACCGACATGGTCTACTTAAACAATTCTTTATTTATTATAAGTAGGAATGCACATTTTATGAATATTTG H@A I95J12L21/03/96M14/04/96N11P1RTony Triglia size selectedTGTGTAT 4xtN@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesY Provisional[Left\75606560190^GBBAE_85575A W 12-0003BACAGTTTGGAAACATAAATAAGTCAGTACCTTCTACACTCTCCTTTTF157G6B1F12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@nonymous markerU3D7Tccctatagatgattcttgt TccttgtttaaactcctcatLTcgtTctacattcatctcatatttITcttcctccgtttcttgatt6Tctttttcttcttcctctat actcatttttagtTttttccatgttatttattg2f6f4AtpaseOra‚‚Ã!0Ã!PÃ!Ã"` Ã#pÃ$ Ã$@Ã% Ã% Ã&pÃ&Ã(Ã(pÃ)`Ã)pÃ*pÃ3Ã3Ã4 Ã4Ã5 ÃC0ÃGÃ1b12f1d11r1e1f 1e9f1h112a7f2c10r2e4r2e7f 2f6f2g9r3a8r4b4fZ] BCTACTTTTATAACTATCTACGCTATAGTGTTAATAACAATGGACGTAACCATAACAACAGCGGTAGTATAGATTATTTGAATGATAACGAAGAAAGTGATAACAAATAATAATAATAATAATAATAATAATAGTAGTACTAAAGAAAGAAACAAATAGTAGATC w@A I95J12L21/03/96M14/04/96N10P1RTony Triglia size selectedA46oC annealing@A>    >TAnonymous markerUB8V394 bpWcompleteXYesYFinal[Left\120^H_11 A W 12-0011BTAGATGTGATGGTGTATGTGTAT gY A W 12-0012BTTATTTAAATCTTCAAGTAATTCCAAAGAAGCAAATTATTGAATGTG F147G6H3FAACATCATCTAATAATTTCATTTTTTCCATTTCTAATTCTTTTCTCTTTTTATTTAAATCTTCAAGTAATTCATTATATCTATCCATTCTACTATTTAATTCTTTTGATTTTATTAAGTTATTTCGTTCATTTCTTTGTATTTCTTCTTCTTTGTTTTGTAGATTATTATATTCACATTCAATAATTTGGTTCTTTTCTTTTTCTAAAGATBITCTAATTCTTTTTTCAACATTTCCAATTCATTCTTTTCTTTCATATTATTCAATTTCTCTTCTTCTAACTCT  @A  CTTTCATATTATTCAATTTCTCTTCTTCTAACTCT  @A  nK1. YAC 620 is a multitransformant 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M3/10/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V213 bpYeWcompleteXYesY Provisional[Left\6075225^BGA_111012A W 12-0014BAGAACATTTTATATACACATATGCGATCTTAAGATATAAACATTCG F131G5C12FAAAAGAAATTGAAAAAATAATAGAACATTTTATATACACATATGAAAAACCAATTTTTAAATATTTAAAATGCAAAAAGGTAGATAACGTAAATATATTTTTTTCTTATCGTCTAAATAATAAAAACAAACGAAATGTTTATATCTTAAGATCTATATGTATATATATTATTGAAAGTCAATGTTTCAAA @A I1271 946 492J12L21/03/96M27/03/96N14P14106R3D7 MunI3D7 MunI3D7 MunIH1 -./019:;<=>MNOP^_afg'()*+13'()*+-14 ()*0153-4 5 !"#)*6  !"#%'(./7 !"#$%+,-.8 !"#$%9 !"#$%./3d7  !"#$%'()*+-./0Muni  !"#$%'()*+-./0cl2A45oC annealing@A>    >TAnonymous markerUB83D73D7V189 bpWcompleteXYesYFinal[Left\6075225^BGA_111012A W 12-0015BATTGAAAAAGCAAAGAGAAAAAT CGATTGGTGTGATGGTTGATGTTCF186G7D8RI1271 946 492J12L21/03/96M27/03/96N14P14106R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@I3D7 MunIA45oC annealing@yK@r wAuAz[{A@|MA@PBAK@AK^@AAAR A!BIo"@#AA$%&@'K(AR)A )A *@A+A,B-B A.B B/@CK01AA2A]a[ S42oC annealingTAnonymous markerUB8V327 bpWcompleteXYesYFinal[Left\120^H_11AATATATATATTTGGGATAAAATAAAAAAATGTATATATTTGTATACACCATATAATAATGTATGGTATATCTTTTTAAATATATATGAAGAAATGTCTCTAAGAAAAAATACACTTTGGTCTTTATGTAGTAGTGATATTAAAAATGATATTAATGTAAATATBvAAATAAATCATATTATAAATATCATAATAATATTTCATTTATTAATTATACCGACATGGTCTACTTAAACAATTCTTTATTTATTATAAGTAGGAATGCACATTTTATGAATATTTG H@A I95J12L21/03/96M27/03/96N11P1RTony Triglia size selectediZ ATCCGTACATTTTCTACATCTCTTCAAATGATTATATTTATCATATAATTGATTATTAATATAATCTTTCATAATATTTAAAGCGTCTTTAGCAACTTCTTTTTTTACTTCAAAACAATACATGGGAACAAGCTCCACATCTCTTGTATACGAAAAAGGTAATATTTCTTCTAAGTTTATAACTTGTCTATCCATATTCTGATAATAATTAB5AATATAGGGCTAAATATTTTTTATTCATATATTTTATATATATCATACATGT  @A  I313 95J12L21/03/96M14/04/96N9P41R3D7 MunITony Triglia size selectedA41oC annealing@jhP J12L21/03/96M27/03/96N8P44R3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D7V244 bpWcompleteXYesYFinal[Left\190135^EC_51 A W 12-0009BATTATTAATATAATCTTTCAT CAGAATATGGATAGACAAGTTA F149G1E1FA I341 313J12L21/03/96M27/03/96N8P44 I341 313J12L21/03/96M27/03/96N8P44i A W 12-0008BTAAGTATTTGTTTTATTATTACGCTCCCTATAGATGATTCTTGTTCAF115G2E7FAGAATTTCTTCCACTTTTTGTATTTTTTGTATTTTTTGTATTTTTTGAATTTCTGCTTGATTATAATATTCAAAAGAATTATCATAAGTATTTGTTTTATTATTACGTAAATTTAAACGTGTATCCATTCGATTATGTACATTTTTTTTGTTTTTTTTATTTTTTTGATTATTATATGAACAAGAATCATCTATAGGGATATTACACATATB"TACAATATTCATCAATTGAATTGATAAAAAAAAA@A I341 313TAGGGATATTACACATATB"TACAATATTCATCAATTGAATTGATAAAAAAAAA@A I341 313 Ttatttaaatcttcaagtaa Ttcctcatccttagtatcat/TtgvTtttTttagatgaaaaacaaatgg*TttatcgtacgaaatgaatgBTttcctttatcaccaaacctTttgtcctgctagaaatagaUAaairAaaaaaaattagatgatagtSAaaaacaggaaaaaagagttAaacgtatattcaagtaaagAaagaaattacattattataAaagaagcaaattattgaat AaagcactgtctaaggtatcQAaatatgttacatcatccac,AaatcttgtgcttcatctaaRAaatttaaatattgaagaagAacvAaccattttagattcatcttAAacgtatatacattcaagttJAacgttcgttcattatttcaCAagfaaatggag*eqAAATTTTTAAAAATACTCATGTCAAGAAAACATATCATACAAAAAATTATAGATAAAAATAATTTATCCACACATATGAATGAATATATCGAATATAATTATTATTTAGAATATAATAAAAAATTGAAAAAGCAAAGAGAAAAATTACTAAATCTAATTATAAATAAAAAAGGACAAATTCGAACACTTAAAAGCATAAAAAATATGTAACBmTTATGGAAGAAAATATGGTACAAGGGAAAAATAATATAAATACACATTTTGTTAAAACATTAAAGAAGTTATTAGAACATCAACCATCACACCAATCAGAAAATAACA ?@A I946 620 1092 492J12AAAATTACTAAATCTAATTATAAATAAAAAAGGACAAATTCGAACACTTAAAAGCATAAAAAATATGTAACCATTTTATATACACATATG9[Middle\150125^AC_51:A W 12--0055BCATATGATCATTTGTCAGTTTTT CTATACTTTCCATGCTTCTCTATT F114G7E12RATTTGATAAGCCATATTACTAATATCATATATATGAGCTTTCATATGTTGTTCATTAGACATATGATCATTTGTCAGTTTTTTTAAATATATATTCATTTGATATATATCATACAACCCTTTTCTCTCTTTATATGGATTAACAACTAATAATAGAGAAGCATGGAAAGTATATATTTTGTTTTTCTTATATCTGTTTTTTATATTTTGTAB-ATACATTCTCTACATTTAACTGTATAATATCTATATTGTGTTCG @A I293 25J12  >TACACATATGbrtWcompleteXYesYFinal[ Left Middle11070130225^DCDGA_81211412>TAnonymous markerU3D73D73D73D7V318 bp[ Middle Left\75110225^GDCA_1081212A W 12-0016BAGACATGCATATTCATTCTGTTGCTTTTTCCATGTTATTTATTGATAF79G2C10R0 1092 239 76 225J12KYAC 620 is a multitransformantL21/03/96M27/03/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI !6AC 620 is a multitransformant 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M27/03/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V213 bp* Yac'-./012YacsYk45f10.3C Iw}u { !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abc:dCe^fg_hijhkmlmntopuq{rstu/v Iw{  "#$%&'(/1T+lK1. YAC 620 is a multi-transformant. 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M27/03/96N15P107126R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V318 bpWcompleteXYesYFinalnwL27/03/96M27/03/96N18A W 12-0017BTCATATAAGATGTGTAGGAGTAA CCTTATCACATTATTGGTTGAACA F227G3A8RAAATTAATATTATAGATTTGAAAGGACTAGTGACAATAGAAGTAAAATTATTAAATGGTATATCATATAAGATGTGTAGGAGTAATATCAAGATAGATGAAATATCTAGTTTTTTTGGATCTTCCAATAATTATTCTTCATATAATGATATAATAAATAAGACTATTTTATGTAACTACAATTTGAGTGTTAAGGAAGAAATTGACCTTTTBzTAATAAAAACGATAAATCATTTTTAAAACAAGATAAAGATAAAAATATTTCGAAATGTTCAACCAATAATGTGATAAGCCATAATATTATTAATAATCATTATAATAATA TAATTAGGAGGL@A I620&+ A gA gA gA gA gA gA gA g A  g A  g A  g A  g A  gA gA gA gA gA gA gA gA gA gA gA gg^IAAGAATATTGAGTTTATTAAATTTAAAACATTTCAATCATTAGTTTCATATGATAGATCTACAAAATGTTTATCTGAAAATAGTAAATATCACGTGAACTCTGATAATAACAAAGGTAATAAGTTGTTTGGACAGAAACAGTTTGGAAACATAAATAAGTGTGATGTTAAAGATGATGAATTAAAAATATCTAAAGATAATACAAACAAGABWAAAAGAAAATATGTTTTAAAAAAGAGAAAAGGTCGAATGAAGAAGAGTATAATAATTTAGAAAAGGAGAGTGTAGAAGGTACTTGT  )@A  I464 785 1073 1255 341J12L21/03/96M27/03/96N3P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIwz LA>    >TAnonymous markerU3D73D73D73D73D7V331 bpWcompleteXYesYFinal[ Left Middle11070130225^DCDGA_81211412A W 12-0018BGTGTGCTATTATGACTCTTATTA CCATTCATATTCATTCTGTTGATA F149G2A7FL27/03/96M27/03/96N20A42oC annealing@A>    >[ Left MiddleCe ReverseSSequence-.imtSequencedfwSequencesIwSequencinguSexual[/0Subunit>SurfaceSSwiss!-.:>Synthase!T-complex>T02521)T18056MThe-./0ThereIThisThree-./0To-./0IJKLMNOPQRSTTonyTrigliaTrna!TwoUCU194900001Var-3Var-7SVectorWasIWeWhereIWith!)-.:=>CMPQUWithvariant-specificSWould)*X546310001PX555040001=ru 1092 239 76 492J12 A1. YAC 620 is a multi-transformant. 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker sBL on this YAC 3. STS sequence is reverse and complement of original sequenceL27/03/96M27/03/96N19P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing@^A W 12-0002BTGCCTCTATTGTGTATACAT CTGCCTCTATTGTGTATACAT DORAF360GORAI464 785 1073 1255 341J12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing^FB_12A W 12-0002BTGCCTCTATTGTGTATACAT CTGCCTCTATTGTGTATACAT DORAF360GORAI464 785 1073 1255 341J12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing-3f; @aIYWc@eAlKq[KbWnKq[eAlKq[q[lKq[Kq[AlKq[Kq[AlKq[AlKq[u{AGTTAACATTATTACTATTTTTTACTTTGATTACACCAGGAGGTGTGCTATTATGACTCTTATTAACACTATAAAAAGAGTTAATATTACTACTAGTATTATTTATAGGAATTTTTTTTTCCATGTTATTTATTGATATATCCTTATAAAGGGATATATATTTACCTTTATCAACAGAATGAATATGAATGTCTGTTGATTT @A I239 492J12L27/03/96M27/03/96N20P36R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V201 bpWcompleteXYesYFinalz[ Left Middle\70225^DA_1112A W 12-0019BACGAAACATCAAACTTTTATTAT CTTTATATACTCATTTTTAGTCAT F156G1D11RAATGACGAAACATCAAACTTTTATTATATTATACAATATGAGAATAAAGATTTTCTTACCATAATATTTATATCTCCTATAACCCTTACGACATATTACACGCTAAAAATAACTGACTTCTACTCAGGTACAAAAATATGACTAAAAATGAGTATATAAATATATATGACATGTACAAGCCATATTACATTTTGTTCGATT @A I76 492J12L27/03/96M27/03/96N21P16R3D7 MunI3D7 MunIA42oC annealing@9A W 12-0020BACATCATCTAGATCCCACACAAT CTACAATTTTTAATTCTCCTTTTT F102G5E11FATAGAGACATCATCTAGATCCCACACAATATTTACAGTAATATTAGAATCGAATCAAGTATTAAATGATGGAACATCTATTAATAAAAAAGGAGAATTAAAAATTGTAGATTTAGCTGGTAACGAAAGAGCAGGAAGAGCGGCTGAAGGGATAGAAAA@A I76 492J12L27/03/96ATCGAATCAAGTATTAAATGATGGAACATCTATTAATAAAAAAGGAGAATTAAAAATTGTAGATTTAGCTGGTAACGAAAGAGCAGGAAGAGCGGCTGAAGGGATAGAAAA@A I76 492J12L27/03/96APAnonymous !"#'()*+,-./012345789:;<=>?ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_fgjknqruvwCdna`aClone`aEndsGene$%6@bcimt\RG'()*?@IJKfgiH /01345JKLMNOPQRSjn|NM27/03/96N22P16R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V157 bpWcompleteXYesYFinal[ Left Middle\130225^GA_412A W 12-0021BAATTATAGGACACTTCGTTCAAT CACCAATTATATTCATTTTTCATA F97G8D7FL27/03/96M27/03/96N23A42oC annealing@A>    >TAnonymous marker[ Left MiddleTagagacatcatctagatccTagagattacmTagaggaagatTagatagatatTagatatatatTagatatgactTagatattattTagctcagtctTagctgggtgmTagctggttgmTaggaataagtTaggactaacmTaggagtttcmTaggattaatiTaggtaaatttTatagttaagmTatatataatmTatatggatamTatatgtgcgmTatattattamTatatttatamTatcaaaagamTatcctgaagmTatcgatcatmTatgaatcagmTatgaatgatmTatgatatcamTatgatcggamTatgatgaagmTatgcatctamTatggacaaamTatggtatatmTatgtacaaamTatgtcatacmTattagatca MuniSelected +,-.9:;<=>CDEFGHIVWXYZ[`amrsSize +,-.9:;<=>CDEFGHIVWXYZ[`amrs,743o/43oc "(18:44oc#545oc $%-2346946oc )*;47.47oc'048oc!<49oc50ocAnnealing  !"#$%'()*+,-./0123456789:;?BCDEFGHIJLMNOPQRSTUVWXYZ[\]^`Provisional%)*+0@AKbcfijkmnqrswLeft  !"#$,-.fgijnMiddle !"#$%'()*,-./)*+Ã!-Ã"Ã"`'/Ã#`./Ã$%'()*+Ã$` Ã%`AAATTATAGGACACTTCGTTCAATTGATGAACATACAGAAAAGTCTTTAAAATATTCTATGTCTATTTCAGCTAATATGAAAAATGAATATAATTGGTTAAGGTCTTTAGATACTAATGAATATGATACCAAGTGGGACGGTTTCGATAAATATGATGATCAGAATGCTAAAGAAAAA @A I76 492J12L27/03/96M14/04/96N23P16R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V177 bpWcompleteXYesYFinal[ Left Middle\130225^GA W 12-0022BCAAATCTAATACATACGAATCAT CACTTGCTTTTATAACTATCAATA F185G2G9RAGGATAAAAATATTAACCATATAAAGAATGAAACTGATGCATTAAAATGTAGTAGTTATAAAAAAGAAATCAAATCTAATACATACGAATCATTTACAAACAATTCTTTAATTAATTTTGAAGATAAGAAAAGTAACGATGTTAAGAGTAATATCAGTACTTTATTATTAAATCTATATGAATATAATAATAAAAGTACAGATCAAATACABtTTATTTTGATCGAATTAAATCTATTGATAGTTATAAAAGCAAGTATAAAAATGGTTCATATTTATTGGAATTACACTCAAAAGATAGTGAAAATGATAGTAATAAAAATGATGCA F@A I492J12L27/03/96M27/03/96N2403/96N24P6R3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D7V325 bpWcompleteXYesYFinal[ Left Middle\225^A_12A W 12-0023BCAAATAAATGAAGAAGACAAAAA CCATGATGACTGCTATCTACCTTA F322G8A11RAAATGAAAAATATGATGAAGATTTTACTTTGAATTATATTCTGCAGAAATACGATGATGATGATCAATTTTTTTGTAGCAATATTTATACTCAAATAAATGAAGAAGACAAAAAAGATGAATTAAAAAAATATACAGAAGGAATGAATAGGTTAGTAGAACAAATGTACTTTTATGATAATTTCAATGATGAATATAAAATAACTAAAGAA CaaataaatgaagaagacaaCaaatctaatacatacgaatCaatatttgaatgtaatgtcWCagaatcttttccagttactMCagcttgtaaatctgttgaa5Catatgatcatttgtcagtt:CcafkuCcattggactcttggttctaCCcguCctkCcttatctccattcttgttgnCgtsCtsCtaaacagttgtgctaatacLCtactattctttctaactctaCtcrCtgtggataattcttggtccECttattaaaaattgatgagg^CttgtttataaatgtgatggDGaagGaagataacactgacgggaaYGaatataaatcgtgcttacc`GacgGaggrGatktGcakGctctatctttaataatcatAGgaagcaatataatgagtaaNu|AatgcatcatttcataatatAatggagtggmAatggctggatAatggtattaiAatgtaaagatAatgtaatgttAatgtagatgiAatgttaacatAatgttagttmAatgttcctamAattaaaaagtAattaaaatamAattaaatgttAattaaggaaiAattaataaatAattaataatmAattaatacgtAattaatattatagatttgaAattaattatattaatctaa/AattacgatgtAattagaaaatAattagctatiAattagtaattAattataggacacttcgttcAattattttgmAattcaatatttatattattRAattctttaatattataaac[AattgataccmAattggtataiAattgttatamAatttataaam%\ In3d7InhibitedUIs'./012STUVmItKb0Kinase-L406090001L426360001SLibrariesLightLikely-./0M691470001[MalariaimtMappedMarkerMore0Multi-transformantSTUVmMultitransformant'./012MunMun1-./012IJKLMNOPQRSTUVa-./0P)P31209.P37880!P39077-PcrsPf0131c)Polyadenylate-bindin.PositiveProtein!-.Regulatory-RestrictionaKNo positive YACs in3D7 Mun1 or Tony Triglia B8 YAC libraries but OK in positive control PCRs. The fragment has been mapped by restriction analysis of 3D7 gDNA.L27/03/96M27/03/96N26A45oC annealing@A>    >TAnonymous markerV148 bpXYesY Provisional[ Left MiddleGAACACTTAATTAATTTAGAA @A J12    >BAATAAAAAAGAGACAACTGAATCGTTTGATATTATAGATACATCATTGAATATACATGTTGTTGATGCTAATGAAGATAATATAACAGATGTAAACAAGGATAATCAGGAAATTATTTCAAAAATGGAATTAAAGATAAAAGAGTTGGAGAAAACTATTGATGAAATGAAAAAGAAGGATAAGGTAGATA GCAGTCATCATGA  @A  J12KNo positive YACs in3D7 Mun1 or Tony Triglia B8 YAC libraries but OK in positive control PCRs. The fragment has been mapped by restriction analysis of 3D7 gDNA.L27/03/96M27/03/96N25A46oC annealing@A W 12-0025BTCTTCCTTAAGTGATGAAAATAACA CAGCAGCAAACATTAATGACCTCGT DATPase 4F237GATP4I1347 237 357J12L27/03/96M27/03/96N27P153ysis of 3D7 gDNA.L27/03/96M27/03/96N26A45oC annealing@A>    >TAnonymous markerV148 bpXYesY Provisional[ Left MiddleA W 12-0025BTCTTCCTTAAGTGATGAAAATAACA CAGCAGCAAACATTAATGACCTCGT DATPase 4F237GATP4I1347 237 357J12L27/03/96M27/03/96N27P153 A>    >TAnonymous markerV413 bpXYesY Provisional[ Left MiddleAW12-0024BAAATTAGTTATAGAGAAAAGAGG CTTAATTAAGTGTTCTAAAACTTC F133G7A12FAAAAGAGTTAAATTAGTTATAGAGAAAAGAGGTAGAAAGGGATTAGATAAACATGAACATATTAATATATTATCTAAATTATGTGAAATAGAAAAACAATTAGTACACAATCTTATATAGAAGTTTTAGAACACTTAATTAATTTAGAA @A J12hAgtaccttctacactctcctOAgtatgtagaaactgtgtacAtaaaattattaaatcacaa8AtagatgtcgacaatgttatFAtatttttaaatcagtatgtAtgiAtgaccaccccctttttatc0AtgtttactcatcataggnAttgjsAttaatagatatgtcctgtg2Attcaagcccttcgtaatct=AtttcttctccgttatcaatBCasCaattttaaatatttcagaaqCactuCacagaaagattcataaagcGCaggruCaggcgaaagctatagttcta-Gtagtaataaaacgaaaagt/TacaatttttaattctccttTataatcatagtgtgggtaa+Tcaaataatctatactaccg Tcattattattcgttgaagga4R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D7WCOMPLETEXYesY Provisional[ Left Middle\60100145^ADF_399A W 12-0026BATTTTTTTCAAATATAGAAACTC CAAAGAAATTACATTATTATATCC F104G8E6FL27/03/96M27/03/96N28A45oC annealing @A>     >[ Left MiddleATTAAAATAATAATGTAATATAAAAATTATCAAATAAATCAATTAATCTTTTTCTTATTTTTTTCAAATATAGAAACTCAATAATGATCAAACGTTTATAATTTTTTTTTCTTTTTCAAATTAAATATATAATCGAGGGGATATAATAATGTAATTTTTTTATAATAAAATATAAAAA @A  I237 357J12L27/03/96M27/03/96N28P34R3D7 MunI3D7 MunIA45oC annealing @A>     >TAnonymous markerU3D73D7V177 bpWcompleteXYesYFinal[ Left Middle\100145^DK1. Homology with outer arm dynein light chain December 1995 Genbank protein acc. no. U194900001 2. Could not get a signal hybridizing Y1103 (Mun1 12D11) with pBR - deletion in vector sequence?L27/03/96M14/04/96N29P34126R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V192 bpWcompleteXNoYFinal[ Left Middle\100145225qb[ Middle Left\75110225^GDCA_1081212A W 12-0016BAGACATGCATATTCATTCTGTTGCTTTTTCCATGTTATTTATTGATAF79G2C10RAAATGCATCATTTCATAATATGTCTTCTATATATAAAAACAATGTAATCAAAAACCAAGGTACCTTTCCCTCAGGTAATGAAAAATCAACAGACATGCATATTCATTCTGTTGATAAAGGTAAATATATATCCCTTTATAAGGATATATCAATAAATAACATGGAAAAAAAAATTCCTATAAATAATACTAGTAGTAATATTAACTCTTTTBTAT @A I620 1092 239 76 492J12?y% AZ B` C]Sg% December!)-.:=>CPQSU[DeletionDetectedIDirectionuDirectionsfDivisionUDnaPDyneinEachuElegansCEndswlciparum)M[FoundFoundyac-./0FragmentFrom)*-./0fimtuGamma>GdnaGenbank)=CMPQSU[GeneQGetGlucoseUGroup-./0Grow)*GyrasePHas-./0IJKLMNOPQRSTaHaveHeavy:Homolgy:Homology!)-.=>CMPQSU[HybridizingInuQA W 12-0028BAAATTTAAATATTGAAGAAGAAA CAAATTTAAATATTGAAGAAGAAA F170G1B12F@A>    >TAnonymous markerU3D73D73D73D7V192 bpWcompleteXNoYFinal[ Left Middle\100145225^DFDE_991110A W 12-0028BAAATTTAAATATTGAAGAAGAAA CAAATTTAAATATTGAAGAAGAAA F170G1B12FL27/03/96M27/03/96N30A42oC annealing@   >LxGKEHomology with var-3 December 1995 Genbank protein acc. n.o.L406090001L21/03/96M27/03/96N1P414R3D7 MunI3D7 MunIA50oC annealing@A>    >TAnonymous markerU3D73D7V321bpWcompleteXYesYFinal[Left\7075^FB_12ATTATGAAATGAATTACTTGTATGAAAATTTAAATATTGAAGAAGAAAAGAGAAAGCGAAAATTAAGCAGCCTGAAAAATTATGAAAAAGATTTAAAACAAACCATTAATAAATTAAAGAAATATAAAATTATAAATGAAAATATTCAATATATTAATTTAGATGGTAATGGAAAAGGAGTGTATAAAATAATCGATGTGGATATAAACCAB AAAAGAAAAAG  @A  I538 3 619 724 822J12L27/03/96M27/03/96N30P61789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA42oC annealing@"[Ã( !"#$%Ã(` !"#$%-./0arsuvÃ)VWX`Ã)`13456Ã* Ã3`  !"#Ã4CDEFGHIJKLMNOPQRSijnÃH+,-.a1k4k5k+,-.789:;<=BCDEHISTUVXYZ[abcB $%'()*./01567VWfC  !"#$+-01345679:;<=>TUVWX^_`D !"#$()*+013456?@ACDEFGHIKLMN2SA@[DOFIJJ CGPRIMER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALK@x^At@IWM@TBUBWT @jJi Ah OA>    >TAnonymous markerU3D73D73D73D73D7V221 bpWcompleteXYesYFinal[ Left Middle\22575175175170^EADEE_103746A W 12-0029BATCAGTAAAAATTATTGACCTTT CATATTTTTAAATCAGTATGTGGT F185G7A2FL27/03/96M27/03/96N31A42oC annealing@A>    >TAnonymous markerAATGGTAAGAGATGATGGCTGGGTAGGTTTTGATAAAGCTGAAATTATAAAAGTATTTAATAATAATAGTAAAATTAAAGATGTACTTATACGTAACCTATCAAAAAATGATGAAATTATATCAGTAAAAATTATTGACCTTTATATGGATTATGTAAATAATCATAATCATGGTCATAATCATAATAATAATCATAACAATATTAATATABAATGATGATGACGAAAATAAAAATGATGAAAATAATAATAATGATGATGATATCTTAAAAAGAATTGCTGGACCACATACTGATTTAAAAATATTAAACCAGTTAGCTATAACATATATATGTAAATGGTTAAAATATAATATATC e@A I538 414 619 724 822J12L27/03/96M27/03/96N31P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V356 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_106746 A W 12-0030BTCTTATTATCCTTTTTATTTATG CTCTTTTTCTTCTTCCTCTATGAA F386G8E7F8 414 619 724J12L27/03/96M27/03/96N32P6578R3D7 MunI,- !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvw   @ Find New entryTestFind all/Control ScreenChromosome sort STS Sort Probe SortUpdate for Ross AFind_zTctttttcttcttcctctatTgajsvTgagagtgaagaagctttaa%TgatggacgtctaggaattaKTgcctctattgtgtatacatTgtrvTgtggtggtggttttggtagTgttacaacctattttgcctHTtaisTtaaataatttaaatgatgtNTtaattaagtgttctaaaacTtagttatacagacattcta_TtcgrtTttatatactcatttttagtTttcaataattacatttctgYTttgaatgtagactttttac#TtttcgtcggaggtatttgtPTtttgttcctaatttatcct(ÃIp Ã1b12f1d11r1e1f 1e9f1h112a7f2c10r2e4r2e7f 2f6f2g9r3a8r4b4f ACTTATTATATATTTGTAATATGTATTTCCAAAATTTGAAAGATGATATATATTTTTTTTCTTATTATCCTTTTTATTTATGGCCAGTTGAATTAATGAATTTTATTCATAACTTTTTATTATCAAATTTTAATTTTTTTTATAACGATATTATATCACTTTTTAAAAACTATAAAAAAGTTGGATCATATGAAAAGAAAATATTTTCATCBTAAATATTATCATAATATAAATAAAAAAGTAGACCTTTTTTTGAATAATTTTTATAGCTCACAAGTTGAAGACATGTCCCTAGAATCCTTTATGAAAGAAACTGATTTTTTTAATAATAAGAAACAAGAAGGTGAAGATAATAATTATAATAGTAATATTAATAATTATGATAATATCGAATGTATATCTCTTGATATTTATGGAAAAAACDTTTCATAGAGGAAGAAGAAAAAGAATGTTTACATAAAAATGAAATATCACAAAGAATATTATTAGAA  @A I538 414 619 724 822J12L27/03/96M27/03/96N32P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V487 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_106746!A W 12-003127/03/96o!I538 414 619 724 822J12KNHomology with arginyl tRNA synthase December 1995 Swiss Protein acc. no.P37880L27/03/96M3/10/96N33P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA48oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V378 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_10674!BAAATCAAATATTGTTTCAAAATG CCTATGTTATCGACTGTTCTGGAT F247G7C4FAACTTCGTGTTTTAAATTTTTTATTATCTTCATTTAGAACGAAACCAAATCCTACATGCATTAATTTTGATTTTTATCTCCCCAGTTCGTTTTTTTAATCAAATCAAATATTGTTTCAAAATGTGTTAGTTGACCAATATCAGTAACATATATAACACAATTACAATTTAATTGTGTTAATCTATAATATAATGCAGCTACATCTGTAGAABTCATAACCATATCCCCCGTTCGATTTTTGTAAAAATAATGGTACTTTGAAATTTTCTGATTGATAACATATGGCATCTCCTATATTTGTTAATAATTTACTTTCCTTTAACAAATCCAGAACAGTCGATAACATAGGTACATAGAATGATTCACCAACATACTCTAAT {@A o"A W 12-0032BATTTTCTAAATTACCATTCTTTG CCCTTATTAGAGGATAGTGAACAA F128G5F12FAAATATCTTTATAACATTTTGTAAAGCTATCTACAATTGATGATAAGATTATAAAAATATTTTCTAAATTACCATTCTTTGGAATATCTTCACCTAAATATATACCTAATTCCTTAACATCTTTTTTAGTTTGTTCATATTGTTTTTGTGCTGTATTATATTTTTGTTCACTATCCTCTAATAAGGATACCATTTTTTCCTTAAA @A I538 414 619 724 822 705J12L27/03/96M27/03/96N34P657898R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIJ‚!‚" *-MNOPQRuv‚# -BCDESTUVWX]^_`bck ABHH +7G{HH%.d'@Dw/11/961/97ATTTC CTAGTTAGCC ATAGAATGGT TGATTAGGCA GCCTTTTCAA AAAAAAAAAA AAAAACAACA AAAAAAAACC ACCATAAAAT AAAATAAA"3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V204 bpWcompleteXYesYFinal[ Left Middle\2257017517517055^ECDEEC_1067469#A W 12-0033BGTATACTGGATCTAACTTCTTTT CTTTGAATGTAGACTTTTTACCAA F273G7D9FL27/03/96M27/03/96N35A44oC annealing@/Tttttccatgttatttattg1019c`1033ca11t2%2f6f3'k1cl12c45b60@Abra6Atpase%Clathrin$Cpn@CspiCtrpmK1cl12bOraPoltRnat$u‚J‚H‚X‚+‚R‚4‚K‚3[‚Ã!0Ã!PÃ!pMÃ!Ã"jnÃ"07Ã"@-Ã5 Ã7%Ã8@#ÃC0ÃGÃIp Ãd$Ã1b12f1c10r)1d11r1e1f 1e9f1h111h5r+2a7f2c10r2e4r2e7f 2e8r'2f6f2g9r3a8r3c10r(4b4f#AATAAAGTAAATCCTAAAGCAACTAAATCTTCATTATCATTTATACCATAATTTACATAATTTCCCCATTTTAAAACAGCTTTTAATAAAGATCTTAATCGTATACTGGATCTAACTTCTTTTACAGCTTTATCATATATATCTAAATCTTTATTTATTTCATTAATCATTTTATCATATTTTAAAGAAAATAAACATATTTGTATTTTACBTTTGACATTTATCTAGATATACAAATGGTATTAATTTCCTTTCAACATCTCTGAATTGTTCTGTTTTTATTTTTTCATTTTCTAATTTTGGTAATATACCGTCACATTCTTCTTTGGATGGAATAATATGTAATACTTTTTGGTAAAAAGTCTACATTCAAATCCGTCAGATCAAAAGAATTAATTTTATCAACAATTTCTTGAGTACTCCATTGGAAATCTTAATAAAATACCAATATTT @ #A I538 414 619 724 822 705J12L27/03/96M27/03/96N35P657898R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V450 bpWcompleteXYesYFinal[ Left Middle\2257017517517055^ECDEEC_10674#69$A W 12-0034BTGGTCTCTTCAAATTAGA CCTATAATATGGTACAGTTG DCLATHRINF530GCLATI619 724 822 705 594J12L27/03/96M27/03/96N36P78987R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesYFinal[ Left Middle\1751751705560^DEECB_7469$6%A W 12-0035BATAACAGAACCGTCAACAACT CTGAGAGTGAAGAAGCTTTAAT DATPase 2F260GATP2I724 822 594 495 1122J12L27/03/96M27/03/96N37P897612R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesY Provisional[Middle\1751706080 130^EEBBF_466H[W  !"#$%'()*+,-./012356789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ\]^_`abcgijkmnqrstuwW12-0024W12-00494W12-0088[W3fW3-0015v&L27/03/96M27/03/96N38'A W 12-0036BAGGCCTCAACAAGGACCGAGTAT CAGGCCTCAACAAGGACCGAGTAT F154G2E8RACATGAAATCTCTTTCTTTATATTCAGGTGGTAATTGAAACCACGACTTATTATAAAATCCCATATTGTTATCATCATCATCTAACTCTTCATTCAAATGTAATGTTGAAATAATTTTATCATTATATAAAGTATTCTTTTCCTTTATAACATTATTTTTAATTGCTTCTTCATAAGCACATGCTTCTAACTTGGTTAATATATATTTATTB*TATTTTTTTATCATTATTATCATCATTATCAGTCTTTACTT @A I495 1122 1231 501 1125J12I495 1122 1231 501 1125J12  Aggcctcaacaaggaccgag'Agggggtggtttcaatcctt0AggtgtacacgtaactgctcIAgttagttgatttgatgttt(AtavAtaaaaaatgaacaagttaaKAtaaagacatgaaatcatgtwAtatcgtagaatgtttgcttAtatcttcagatactggaac[AtattacaaggtttaggaggAtattatgcttcaaagattc]AtattcatacaatttctgagAtcvAtcagtaaaaattattgaccAtgijtAtggctttctatactgtttcSAttfgsAttatatttaaagttttcagTAttattaatataatctttca AttgaaaaagcaaagagaaaAttttctaaattaccattct"AtttttttcaaatatagaaaCfkCagCaafst'K YAC 1125 is a multitransformant.L27/03/96M27/03/96N39P61213612R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA47oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V251 bpWcompleteXYesYFinal[Middle\8013055115^BFGBF_36799(A W 12-0037BAGTTAGTTGATTTGATGTTTTTA CTTTTGTTCCTAATTTATCCTTTG F142G3C10R@(AGCCACCATAAATTATTATCTCTAATTTCTTTGGGTAATTGTATACATCTTTCGGTATCTTCATTTAATGCTTCAAAATGAAAAGTTAGTTGATTTGATGTTTTTAACAATGGTACATATTTTTCTGGATATTCAACCATTAAGTGTACATCAAAAAAAATACTTGGGGTATATTTTTTTAAATTATTAATAACAGGTGGACCAAAGGATABAATTAGGAACAAAATGCATATCCATAACATCTAAATGGATCCATTCTGCACCCAAAGATTCCATTCGTTGTGTTTCTCCAGCTAATTGGCTTATATTGGATGCAAGTACTGAAGGGGAATTATAGCTTTTTAACGTACCCATCTCCAGCA  i@A  I495 1122 1294 1231J12L27/03/96M27/03/96N40P6121413z(R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing @A>     >TAnonymous markerU3D73D73D73D7V360 bpWcompleteXYesYFinal[Middle\801308055^BFDG_36107)A W 12-0038BGTATTAAATTACTTTCGGGTTCC CAATGACAGGGTCAGATGAAGAAT F140G1C10R27/03/96M27/03/96N41A46oC annealing@A>    )ACTTTTTCTTAAATGTTGTCAAATGTATTAAATTACTTTCGGGTTCCCAAGTATTCTCATCATCTGAATATCCTTTCCATTTTACTAAATAAATAAAACCATTCTTCTTTTTTTTTATTTCAAGTATATCACCAATTTCAAATTCTTCATCTGACCCTGTCATTTTTTCTTCTTTATTATAAAACTACTTTTATTTTGTGTACAAGTAGGTBATATTGTGTATGTGTGTTCTTTC @A I1122 1294 1174 461J12 A1. Homology with P.falciparum cDNA clone Pf0131C December 1995 Genbank Nucleic acid acc. no. T02521 2. Y461 (Mun15G5) would never grow from our -70oC stock * A4Y461 (Mun15G5) would never grow from our -70oC stock4@A> ,  - >L27/03/96M14/04/96N42P121413514R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA46oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V356 bpWcompleteXYesY Provisional[Middle\130809045^FDBG5*G7D4RACGTGATAAAGAAGAAAAGTTACAAGAAAAATTAAGAATGAAACAAATAAAGAAGCTTTTAGATGAAAAACAAATGGTTATATGTAAAATTGAAGATATAGCTAAAAAGAGAAATAAAAAATATGTAAAAGCACAAGTATTATCTAAAGATTCTAAAATTGAACGAGCCTATTTTAGTCATAAAATGAAAAAATTTTATAATTCCAAATCTBAGCTATTTTGGTTGTGGATGGTCAAGTAACAAAAAGGAATGGACTCCATTTATTCATGCACCTTTCTTTGAAAAACAACATAACGCAATTTATAAAAATAGAAATAAAAAATTGTATGAAGAAATATATGATACATTATTACATGG e@A I1122 1294 1174 461 1295J12 >hCgtgataaagaagaaaagtt*CgtgtcttaamCgttgataatmCtaaaacttgmCtaatatatatCtaatccagtmCtacacataatCtaccctttttCtacgatatgmCtactattcamCtactttcattCtagtaaaaamCtagttagccmCtataaaagctCtatacctcctCtatgacatttCtatgcaaaamCtattattaatCtcaacaaatmCtccagttttmCtccatcaactCtccttaatccaatatttgaWCtctgaatacmCtgagagtagtCtgatttgatmCtgcaagtatmCtgcatgttcmCtgctgctgamCtggaaaattmCtgtggataattcttggtccECtgttatatgmCttacggatamCttagcagcamcgaf +A>    >TAnonymous markerU3D73D73D7B8V160 bpWcompleteXYesY Provisional\1303090370^FDCA_661067V356 bpWcompleteXYesY Provisional[Middle\130809045^FDBGD_61010511+A W 12-0040BTAAAGCTTATATTAAATGGTTGC CTATAATCATAGTGTGGGTAAAAT F83G1H5R22 1002 1186 582J12L27/03/96M27/03/96N43P1211137*D_61010511+A W 12-0040BTAAAGCTTATATTAAATGGTTGC CTATAATCATAGTGTGGGTAAAAT F83G1H5RAGGGTACCTTGTTGGATTTCTTTAATAAAGCTTATATTAAATGGTTGCGATTTTTAAACTTAAAAGAAAAGACGGATGAACTTATATTTTACCCACACTATGATTATAAATTAAATATACCTTCTGTTCTTAATTTTGTAAAATCAAAAAAAAAAAAAATT @A I1122 1002 1186 582J12L27/03/96M14/04/96N43P1211137R3D7 MunI3D7 MunI3D7 MunITony Triglia size selectedA41oC annealing@%,A W 12-0041BTTAAATCATGTTGATACAAAAAC CAAATATGTTACATCATCCACTTT F232G8B10FACAGCAATAATAAGATGTTAAAGGAACTGATTCCCCCCTTGGAGGCATCGTTTAAATCATGTTGATACAAAAACATTTTTAAGAATATAAGTATATATTTTATTAATGGATTTTTTTGTATATAGGTTTGTGTTTGTATTGTACTTGTGAGGGCACTTAGTTGATTAAAAGAAATATCAACCGATAATTTTGTATAATTAAAAAAGCATTTATGGATTTTTTTGTATATAGGTTTGTGTTTGTATTGTACTTGTGAGGGCACTTAGTTGATTAAAAGAAATATCAACCGATAATTTTGTATAATTAAAAAAGCATTTCBCCATTGGACTCTTGGTTCTATCC CAACGTTCGTTCATTATTTCATTT F234G6H2RATTTTAATGGATCTATAATTATAGCAAAGTCTCAAGATGGACCAAAAGAAAACAAGTTGATATATTTTCTTTCGATTTAATTCTTATACCCTTACACGTGGGTGGAAACCATTGGACTCTTGGTTCTATCCATATGAAAGACAAAAAAATATGTTTATATGATTCTTTGAATGGATCAAATAAAAAGTTTTTTGAATATATGAGAAGATATBATAGTTGATGAAATGAAGGATAAAAAACAAAAGGATTTGGACATATCCTTATGGACTTATAGTAAAGAGGGCGTTTCTGAGGTATCTTAATATATAAAAAGACTGAATAAATGAAATAATGAACGAACGTTTAATAGTATGATGTATATAAATATTATTATATATATGTATATGTATATATATATGTATATATGT @%36sF_99A W 12-0027BTGATTAGCACAATCAATAGCATC CAAAAACAGGAAAAAAGAGTTCTC F126 G5D9FATTCCTTTAATAAAAAAAACAGGAAAAAAGAGTTCTCAATTATAAAAAAATGGCTGATAGAAAATCTAATAAAAATGCAGTTGTAAAAAATGTAGATATGACAGAAGAAATGCAAATTGATGCTATTGATTGTGCTAATCAGGCCTTAGAAAAATATAACGTCGAAAAAGATATTGCTGCACATATAAAAAAG @A I237 357 1103 538J12{| A>    >TAnonymous markerU3D73D7V200 bpWcompleteXYesYFinal[ Left Middle\130225^GA_412d‚U,‚V-‚W.‚X/‚Y0‚Z1‚a2‚b3‚c4‚d5‚e6‚f7‚g8‚h9‚i:‚j;‚q<‚r=‚s>‚t?‚u@‚vA‚wB‚xC‚yD‚zE‚F‚G‚H‚I‚J‚K‚L‚M‚N‚O‚P‚Q‚R‚S‚T‚U‚V‚W‚X‚Y‚Z‚[‚\‚]‚^‚_‚`‚a‚b‚cÃ!dÃ! eÃ!0fÃ!@gÃ!PhÃ!`iÃ!pjÃ!kÃ!lÃ!mÃ"nÃ" oÃ"0pÃ"@qÃ"PrÃ"`sÃ"ptÃ"uÃ"vÃ"wN% ‚#Ã#`a 0424cM1921125'1212c1214-./018501995!)-.1f9-./02)-./03.03d70548./01262069-./06f8./01270oc)*81209d80A'-./012Acc!)-.Acid)AdditionalAnalysisAndArginyl!ArmB8Be-./0BeenBelieveButByCamp-dependent-Cdna)Chain-Chimeric-./0Chromosome-V-BAGAGCTAATTCTCCAAAGTAATC CGGAGAAGAAGGAGATACATTTTT F113G5H12FATTTTATAATAGCAGCTCTTGGTTTATTTTTAAGAAGAGCTAATTCTCCAAAGTAATCTCCCTTTGTATAAGTTTTGATGACTTTATTATCTTTAGAAGCTACAGGATTTCCATCTATAAGGATAAAAAATGTATCTCCTTCTTCTCCTTCTTTAATAATAATTTCTCCATCATTATAACTTTT @A I582 36 243 1211 69J12   >TAnonymous markerUB8V369 bpWcompleteXYesYFinal[ Left Middle^A_6-A W 12-0042BAGAGCTAATTCTCCAAAGTAATC p   )*+,-.3456>?@ABCDEFGHIJKLMNOPQRST02WXYZ[\]^_`abc:dC!789:;<=UVe_hmltop1fgijkmnqrstuvvvvv0@P`p  ‚! ‚" ‚$ ‚%‚&‚(‚)‚*‚1‚2‚3‚4‚5‚6‚7‚8‚9‚:‚A‚B‚C ‚D!‚E"‚F#‚G$‚H%‚I&‚J'‚Q(‚R)‚S*‚T+ jÃ"@Ã"P:Ã"` Ã"ZÃ#qÃ#`0Ã#pÃ#"Ã$ Ã$@UÃ$p\Ã%)Ã%0(9Ã%`TÃ%p/Ã% WÃ% Ã&gkÃ&P'Ã&pSÃ&OÃ&LÃ&QÃ'vÃ'@DÃ'p;Ã(Ã( BÃ(PPÃ(pÃ)`Ã)pÃ)1Ã*rÃ*@?Ã*P]Ã*pÃ1wÃ1P2Ã2aÃ2*Ã3`Ã3pEÃ3Ã3Ã4 Ã4 FÃ40,Ã4PC1b12f1c10r)1d11r1e1f 1e9f1h111h5r+2a7f2c10r2c3f72e4r2e7f 2e8r'2f2f.2f6f2g9r3a8r - >TAnonymous markerUB83D73D73D73D7V183 bpWcompleteXYesYFinal[ Left Middle\3708550100175^ACEEF_6123119.A W 12-0043BGTTATGAGAGATGAAAAAGAACA CAATTGTGGTGATTGAGGTGATGC F257G2F2FAGATGATATTATGTTAAGAGAATTATTTGAACCATTCGGTACTATAACATCAGCAAAGGTTATGAGAGATGAAAAAGAACAAAGTAAAGGCTTTGGTTTTGTTTGTTTTGCATCTCAAGAAGAAGCAAATAAAGCAGTAACAGAAATGCATTTAAAAATAATTAATGGAAAGCCATTATATGTAGGATTAGCAGAAAAAAGAGAACAAAGA- A1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. Sequence homology with cAMP-dependent protein kinase regulatory chaB.in December 1995 Swiss Protein acc. no. P39077 L31/03/96M14/04/96N45P713131RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing @A>    .BTTATCAAGATTACAACAAAGATTTCGTTTGCATCCAATTAGACATCATATGAATAATCCATTAAATACCCCAATGCAATATGCATCACCTCAATCACCACAATTACAATTTAGTCAGAATACATTAAGTTATGG Y@A I582 69 790 548J12 A1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. YAC 548 (Mun1 6F8) is a multitransformant 3. Sequence homology witAGCCATTATATGTAGGATTAGCAGAAAAAAGAGAACAAAGAH12-0043.12-0044/12-0045012-0046112-0047212-0048312-0050512-0051612-0052712-0053812-0054912-0056;12-0057<12-0058=12-0059>12-0060?12-0061@12-0062A12-0063B12-0064C12-0065D12-0066E12-0067F12-0068G12-0069H12-0070I12-0071J12-0072K12-0073L12-0074M12-0075N12-0076O12-0077P12-0078Q12-0079R12-0080S12-0081T12-0082U12-0083V12-0084W12-0085X12-0086Y12-0087Z12-0089\12-0090]. BKh polyadenylate-binding protein December 1995 Swiss protein acc. no. P31209L31/03/96M14/04/96N46P7196RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunIA47 oC annealing@A>    >TAnonymous markerUB83D73D73D7V344 bpWcompleteXYesYFinal[ Left Middle\370175125^AFBF_69108I3D7 MunI3D7 MunIA47 oC annealing/K1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N47P1916R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43o C annealing@A>    >TAnonymous markerU3D73D73D73D7V240 bpWcompleteXYesYFinal[Middle\175125115^FBH Chromosome./0Chromosome14-./0ChromosomesIJKLMNOPQRSTaClone)MComplementConfusionContaining-./0ControlCouldDatabaseimtDecember)/A W 12-0044BTTCCTCATCCTTAGTATCATAAT ecember 1995 Swiss protein acc. no. P31209L31/03/96M31/03/96N46P7196RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunIA47 oC annealing@A>    >TAnonymous markerUB83D73D73D7V344 bpWcompleteXYesYFinal[ Left Middle175125^AFBF_69108/A W 12-0044BTTCCTCATCCTTAGTATCATAAT l/CGTAGTAATAAAACGAAAAGTTGCF146 G6B9RAAATTAATTATATTAATCTAAAACAATCAATTATAAATTAAAATAAAATGATAAAACCAAAATAATAATAAATAAATATGGTTTCATTCCATATTACCAAATTATTATATTATATTATATTATATTATATTTTGAATTGATTAAAATGAATAAATAAATAAATATCATATATATATATATATATATATGTATGAGAAAGATATAAATAAATBATCATACTGATATATATATTTTATATGTAT @A I69 790 91 548J12O/F_910780A W 12-0045BAGGGGGTGGTTTCAATCCTTTTT CATGACCACCCCCTTTTTATCAAT F125G8H4FAAACATTTAATATTTTTTTATGTTGTGAGGGTATTGATCAAATTAGGGGGTGGTTTCAATCCTTTTTTTTTGTTTTTTTTTGTTTAAATTGGTTAAACCAAAGGAAAAAAAAAAGGTCCCAATTATTGAAAAATACTGATAAAATAATTGATAAAAAGGGGGTGGTCATTTTAAAAAGGGAAAAAATAAAAATTAATTTAAAAAATTATACB/CTTAATTAATAATAATAATAATGATAATAATAATAATAATAATAATAI1266 69 790 91 548 603 788 1196J12167913R3D7 MunI3D7 MunI3D7 MunID7fCatgatgactgctatctaccCattcatattcattctgttgCcajCcatttgtttgtactacgtt`Ccttattagaggatagtgaa"CcttctcattcagttcaaaaDCgafsCggtataattaataaatgaa CgttaaggaatatactcataWCgttccatgtcttctcatct>CtagvCtaaaggaatagaggactgg4Ctataatatggtacagttg$Ctatgttatcgactgttctg!CttgstuCttatcacattattggttgaCttttcaaagttttctgtgc;GuGactgtgtctttttcatctgGatfGatccaatataccatcagcaGatcttaagatataaacattGattggtgtgatggttgatgGctfikGgafGgagaagaaggagatacatt-" Middle0123456789:;<=>?@ABCDEFGHIJKLMNOPQRabcjkmnqrstRightBCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bctuvw‚A+‚FVW‚Q0u‚V*MNOP‚a-?@‚f"#$'(j‚q $%34567‚v345‚ !"#01567k‚[]^_`‚%'()*j‚-01\]^fk‚)*+?@A‚?@mqÃ!-BCDEMIJKLÃ&789:;<=Ã&`KLMÃ'`013d110vw11+TUu12%'()*+M_mquv13'()*+-013STUV]^_jn14 ()*0_u15?@AKLMSTfgi234567JKLMNOPQRS]^fgjknst127 !"#$%+,-.018 !"#$%9 !"#$%./013d7  !"#$%'()*+-./012Muni  !"#$%'()*+-./012N  ÃÃ`?@ÃÃ013456Ã0 !"#$%Ã0vwÃpÃ@uÃpTUÄ!+Ä!wuÄ!Ä!uvÄ!Ä"Ä"mqÄ"'_Ä"(MÄ"3%'()*+Ä"6'Ä"qSTUVÄ")*Ä"+Ä"_Ä"013Ä#"-Ä#5]^Ä#B'(Ä#SjnÄ#fPP1A W 12-0046BTCTAAATGACTCTCTTCAGAAAT CACAAATAAAACAAATAAAACTTA F187G6B2Fng@A>    >TAnonymous markerU3D73D73D73D73D73D73D73D7WcompleteXYesY Provisional[Middle\401751251158570165^BFBHFCBD_6910783881A W 12-0046BTCTAAATGACTCTCTTCAGAAAT CACAAATAAAACAAATAAAACTTA F187G6B2F 548J12L31/03/96t1A43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V218 bpWcompleteXYesYFinal[Middle\1158570165185^HFCBDD_7838882A W 12-0047BAAACTAACAACGAAAATGCTGTC CATTAATAGATATGTCCTGTGTGG F204G3F6F8J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N50P6R3D7 MunIA45oC annealing@v  Mun1Mun15g5)*Myosin:NNever)*No!)-.:=>CIMPQSU[NotNucleic)MNucleolar=OOfOkOnIOne0uOrOrderedIfuwOrf[OriginalOur)*OuterOverlapIwOxford-./0P)M[P31209.P37880!:P39077->PbrPcrsPf0131c)Polyadenylate-bindin.PositivePrimersIfuwProliferation-associ=Protein!-.:=>CPQSU[RIReactionsuRegulatory-Restriction1ACTTAGTATTATCTAAATGACTCTCTTCAGAAATTACTTTTAACTGTATTTTTTTTTCCTTTTTATTATAATGTACATCACTACTTTTTTCATGTTCATAAGATGAAGTACTATGATAATAACCATAGAATAATTTGAATAATTTTCACGGTGTTTTTTCGTTTTTATAGAAAAATAAGTTTTATTTGTTTTATTTGTTTTATAATTTTTAB TTTTTTTT @A I91 548 603 788 1196 812J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N49P1679139R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI*r3A W 12-0048BTATTTTCTATTTTTTATGTGTCC CAATTTCAACAATCTTATTCATCC F96G5C11FAvATTATTATCATCATATATGTTATTTTCTATTTTTTATGTGTCCTTTTTTATGTTTACACTATCTTGATTAATATCTCTAATGTCCGTTGTGCACGTGGATGAATAAGATTGTTGAAA v@A I1196 812 475 121J12L17/04/96M17/04/96N51P13952R3D7 MunI3D7 MunI3D7 MunI3D7 MunI196 812 475 121J12L17/04/96M17/04/96N51P13952R3D7 MunI3D7 MunI3D7 MunI3D7 MunI,BAATAATGGGTACTTTTGATGTACAATTTTTTTAATACTAACATTTTCAAAAAGTGGATGATGTAACATATTTTTATATAATTTTCTAATATTAATTAAATCTGATTGTATTGTATTATATATACATATATCAATATCTGAGTTATATATATCTATATCA r@A I582J12L31/03/96M14/04/96N44P7RTony Triglia size selectedA42oC annealing@A>    >TAnonymous markerUB8V369 bpWcompleteXYesYFinal[ Left Middle\370^A_6-A W 12-0042Wj{TAnonymous markerU3D73D7B8V256 bpWcompleteXYesYFinal[Left\190135120^ECH_5111A_412 ) @A>  >L27/03/96M14/04/96N41P1214135R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA46oC annealing@A>    >TAnonymous markerU3D73D73D73D7V233 bpWcompleteXYesY Provisional[Middle\1308090^FDBG_610105*A W 12-0039BTTTAGATGAAAAACAAATGGTTA CAGGTGCATGAATAAATGGAGTCC F217f9 ) @*G A+A,@B-B A.B B/@CK0KOPA2A3A4@AA5BT6\7@8B[9B[:K;AT<A=AK>AK?AA@[BACBADFEFA T GB[HAIBAAJJKKLB 1)-./012>IKLMNOPQRSTUV[m10921125'12IJKLMNOPQRSTa12c1212d1113IJKLMOPQRST13a8STUV14-./0a15d6KLM18501995!)-.:=>CPQSU[9d80A'-./012Acc!)-.Acid)AdditionalAnalysisAndArginyl!ArmB8Be-./0BeenBelieveButByCamp-dependent-Cdna)Chain-Chimeric-./0Chromosome-+^DFDE_991110*2AAGGACCTCGTGCTCTTCAGGTTGCTCGGTGTGTAAGTACCAAAACTAACAACGAAAATGCTGTCGCAAATATTATATACAGAGTTTTATCTGGAAGGCGCTCTTAATTAAAAAAAATATATATGTATAAAACAAGCAAAATTGTATTATATATACACAAATATTTATGTGTGTGTTTAAAAAATATATATATATATAATCACAATGTCCCBZAATAAAACAACCCCACACAGGACATATCTATTAATTTTATATAATATGTATATATATATATATATATATATATATATGTGTGTATATGTG,I548J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M07/05/96N50P6R3D7 MunIA45oC annealing@_)23-?@HIjmrs 56789:;<=IJKLSTUVW\]^km5 !"#)*345IJKMNOPQRVWXYZ`j6  !"#%'(./012TUVgij7 !"#$%+,-.01BCDEFGHIXYZ[`abc8 !"#$%9 !"#$%./013456?@3d7  !"#$%'()*+-./0123456789:;<=?@AN4AW12-0049BGGTCATGGTGATGATAACAATGT CCTAAAGGAATAGAGGACTGGTAT F91G8G2R >TAnonymous markerU3D73D73D73D7V117bpWcompleteXYesYFinal[Middle\1651856560^DDHC_88714AW12-0049BGGTCATGGTGATGATAACAATGT CCTAAAGGAATAGAGGACTGGTAT F91G8G2RL17/04/96M17/04/96N52A45oC annealing@A>    >TAnonymous marker[Middle4AAAAATACGACCACACAAATGATACAACTATTAATAATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATGATAACAATGTTGATAAGGGTACACACAAAAACAAACCAATBEACAATTACAAAATAAATACCAGTCCTCTATTCCTTTAGATAATGAAAGAAAAGAAAAAGAAAAAAAAA  @A  I812 475 121J12L17/04/96M17/04/96N52P952R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@5BTGCTCTTATACAATCTTTTAAAGTACACTGTCTATTAACTTTTTTTAAGATAACATCATCTATATGTTGAATACCTAATTGAACTCTTGTACAACCATAATACCTTAATCTTAGTAATTCATCTTTATTAATTTGATCAGGTCTTGTTTCTAAAGTTAAACCTATAATTCTGCAATTACTTTTTTCATTAATTTCTTGTTCTTCT @A I812 475 121 305J12L17/04/96M17/04/96N53P9524R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    > 4A>    >TAnonymous markerU3D73D73D7V278bpWcompleteXYesYFinal[Middle\1856560^DHC_8715A W 12-0050BCAGCTTGTAAATCTGTTGAACTT CAAGATTAGGGTATTATGGTTGTA F239G7A11FAATATGGTTTATATTCATGATTATTATACCATTTTTCAATTTTAGTAAATGGTGTAATTTCACAAGGATAAATTTTCCATTGATCAGCTTGTAAATCTGTTGAACTTAAAACATATTTAAACATATGAATATCTTTATATACATCAGAATATGGTAGATCAGGCATTAGATGAATATCAACTTTAAATCCATTTTCTTTTAATAAATAAAT3A45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V117bpWcompleteXYesYFinal[Middle\1651856560^DDHC_8871(:Ggtcatggtgatgataacaa4GgttggaaagagaagttcagQGtuaaattactttcgggt)GtgtgctattatgactcttaGttatgagagatgaaaaaga.Taaagcttatattaaatggt+Taagtatttgttttattatt Taatgattctaatataaaga9Tagatgtgatggtgtatgtg Tattttctattttttatgtg3TcatataagatgtgtaggagTccattattactttcttgtg7Tctaaatgactctcttcaga1Tcttattatcctttttattt TcttccttaagtgatgaaaaTgattagcacaatcaatagcTgcctctattgtgtatacatTggagaaaagaaattaggaaTggtctcttcaaattaga$Ttaaatcatgttgatacaaa,kAagattagggtattatggtt5AatjuvAataaattatctaacaaaca[Aatacatatgatgatacatt]AatataaatacgaaacattgEAatgacagggtcagatgaag)Aattgtggtgattgaggtga.Aatttatattattctcattg\Aatttcaacaatcttattca3AattttagatgcttatccaaZAckAcauAcaaataaaacaaataaaac1AcataattattttaaccaagAccsAccaattatattcatttttcActtgcttttataactatcaAgaikvAgaatatggatagacaagtt AgcagcaaacattaatgaccAggcctcaacaaggaccgag'Aggtgcatgaataaatggag*5TAnonymous markerU3D73D73D73D7V415 bpWcompleteXYesYFinal[Middle\185656070^DHCB_87156A W 12-0051BACCAGAACCTACTGTTACTAATGA CTCTTCCTCCGTTTCTTGATTAAGA DABRAF730GABRAI812 121 305J12L17/04/96M17/04/96N54P924R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D7WCOMPLETEXYesYFinal[Middle 6\1856070^DCB_8157A W 12-0052BTCCATTATTACTTTCTTGTGTTA CGTAGGTAGCTTTTTGATTCATTA F112G2C3FAAAAAAAGAATATTGAAAGTATACAAAATAAGAAACACTTAATTTATAAAAACAAATCATATAATCCATTATTACTTTCTTGTGTTAAAAAAATGAATATGTTAAAAGAAAATGTTGACTATATTCAAAAAAATCAAAACTTATTTAAAGAATTAATGAATCAAAAAGCTACCTACTCTTTTGTTAATACCAAAAAAAAA @A I121 305 293J12L17/04/96M3/10/96N55P244R3D7 MunI3D7 MunI3D7 MunIA42oC annealing 8BCACTTGCCAAAGATATCAATATTTGTTTTTTATCATCAACATAACATTTTAATACTTGACTTTTATGACCTATAAATGTTGTAAAATCTTTTAAATTCAAAAACATTTCATTTGTAACAACGTTTTGTGATTTAATAATTTTATTTGTTTCTAAATGTAGATAATTAATATTAAGATGATGACTATCATTAAAATCCTCATCACCCGGGACTTATTATTATCACCACTTGATTGG  @A  I293J12L17/04/96M3/10/96N56P4R3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D7V444 bpWcompleteXYesYFinal8[Middle\150^A_5CAATATTTGTTTTTTATCATCAACATAACATTTTAATACTTGACTTTTATGACCTATAAATGTTGTAAAATCTTTTAAATTCAAAAACATTTCATTTGTAACAACGTTTTGTGATTTAATAATTTTATTTGTTTCTAAATGTAGATAATTAATATTAAGATGATGACTATCATTAAAATCCTCATCACCCGGGACTTATTATTATCACCACTTGATTGG  @A  I293J12L17/04/96M17/04/96N56P3R3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D7V444 bpWcompleteXYesYFinal$9A W 12-0054BTAATGATTCTAATATAAAGAGAG CTATTATTATGAATAGACATGTCG F142G6A9FAATGTGTGTAATGATTCTAATATAAAGAGAGATTATTTTAATTTGAATTTTTATCTTGATTATTATAATAAAAAGAAGAATGAATTTTTAAATGTGGCTATAAATCATGATGAAGATAAGGAGGTGAACGACATGTCTATTCATAATAATAATAATAATATCTATAATGATAATATCCATATGGATAATAATAATAATAATAATGATATCCTAATAATAATAATAATGATATCCF8m9BATAATTATAATATCCATAATAATGATATCCATAATAATAATGATATATATAATAATAATATTCATAATAATAATATCCAAAATAATAATATCCAAATAATAATATCCATATTAATAACCATTATAATTATAACTATAATCATTCTAGTGATAACATTATAAATGAAAAGTGTACTCATCATGCGACAGTAC @A I293 25J12L17/04/96M3/10/96N57P41R3D7 MunITony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D7B8V402 bpWcompleteXYesYFinalm:KOHomolgy with Myosin heavy chain 95F Swiss protein acc. no. P37880 December 1995L17/04/96M3/10/96N58P41R3D7 MunITony Triglia size selectedA43oC annealing@A>    >TAnonymous markerU3D7B8V254 bpWcompleteXYesYFinal[Middle\150125^AC_51;A W 12-0056BAAAATTAAATAACCGTGGACTAT CCTTTTCAAAGTTTTCTGTGCTAT F166G3E3R25J1212-000112-000212-000312-000412-000512-000612-000712-0008 12-0009 12-0010 12-0011 12-0012 12-001312-001412-001512-001612-001712-001812-001912-002012-002112-002212-002312-002512-002612-002712-002812-002912-0030 12-0031!12-0032"12-0033#12-0034$12-0035%12-0036'12-0037(12-0038)12-0039*12-0040+12-0041,12-0042-_Tatactttccatgcttctct:TatctatattttccccctccTTatgaaggtggtttccatttMTattattatgaatagacatg9Tattttccacgatttcttta?TcagTcaaataatctatactaccg TcaaatgcatctttattttcVTcaataaaaggattagaaaaUTcattattattcgttgaaggTccf  19951f9-./0a2)-./0IKLMPQSTUm234120003C2h1JKLMNOPQRST3.0MS3d703g2I548./0126JKLMNOPQRST62069-./06f8./0127S70oc)*812095f:9d80A'-./012STUVmAcc!)-.:=>CMPQSU[Acid)MAdditionalAfg3QAnalysisAndIJelieveButByCCCamp-dependent-Cdna)CChain-:Chimeric-./0Chromosome-;AATTTTTTAATTATGCTGTTATGGATATCAGTATAAAAGAGGAACAAAAGAATAATGATTTCCTTACAAAATTAAATAACCGTGGACTATTAAAAGAAATGTGGGGATATTCATCTTTCCCACAGGGTATCTCATTATTATATTCAAATCGATTATCTACATATCCTCATTGGAGAATATATACATGTACGGATCTTATGCAATTAAGTAAB'TAGCACAGAAAACTTTGAAAAGGGAAAAGAGAAAAGAGA @A  I293 25J12L17/04/96M3/10/96N59P41R3D7 MunITony Triglia size selectedA46oC annealing@A>    >;TAnonymous markerU3D7B8V249 bpWcompleteXYesYFinal[Middle\150125^AC_51 <A  I293 25J12L17/04/96M3/10/96N60P41R3D7 MunITony Triglia size selectedA48oC annealing@A>    >TAnonymous markerU3D7B8V284 bpWcompleteXYesYFinal[Middle\150125^AC_51=A W 12-0058BAATATCAGTGGGAAAATGTGTTA CATTCAAGCCCTTCGTAATCTTAT F300G3A9FCGATCAAACACTAAAAAATATAAAAATTAATAAAATAATTATTTCTATATATTTCA @'&O11PA W 12-0077BAGAAGATGACGGAAGAGGTATCC CTTTTCGTCGGAGGTATTTGTATT F174G4F6RAATATTATTTGAAATTATTGATAATTCAGTAGATGAATATAATAATTTTGAATGTAATACAACTAGAGTCGTTATACATCAAGATGATAGTGTTACTATAGAAGATGACGGAAGAGGTATCCCTTGTGATGTTCATGAGGAACCCACAAAATCTGCCTTAGAAACAGTTCTTCCTGTTCTGCATTCAGGAGCGACATTTTTTGATGACGACBmATGGATGATGATATTGATAATAATATGAACAATACAGGTAATACAAATACCTCCGACGAAAACAATCGTGATAATATATCTAATGATAATAATAATAATAATAATAAAA?@A I181 479 40J1262 bpWcompletete< YSizeTony +,-.9:;<=>CDEFGHIVWXYZ[`amrsTriglia +,-.9:;<=>CDEFGHIVWXYZ[`amrs41oc +W42oc ,7LR]43o/43oc "(18:?N[\445ocv44oc#5DMOT^450cf45oc $%-23469>ABHJKSUV50ocIPAnnealing  !"#$%'()*+,-./0123456789:;<=>?@ABCDRB8<=>CDEFGHIVWXYZ[`amrsÃ! JÃ"3Ã%Ã%ZÃ&Ã'+Ã' kÃ'jÃ(Ã(BÃ)@-Ã)Ã)Ã*TÃ*0Ã*pUÃ*7Ã1Ã1 Ã1@RÃ1P"LÃ2@HÃ2PDWÃ21Ã3 Ã4@)Ã5/Ã5P Ã5;qÃ6 'Ã6P:Ã6pÃ60Ã70 FOÃ7Ã8@vÃ84Ã90 ]Ã9P<Ã9pSÃ:PEÃ:pÃ:aÃA2ÃArÃB0QÃB`\ÃBÃBPAgtttttataattggattga8AtaaaaaatatAtaaaaatgatAtaaaatacamAtaaaatgagtAtaaacgaaamAtaaagagaamAtaaagatggtAtaaagtaaatcctaaagca#AtaaatagtatAtaaatctaatAtaacagtagmAtaagataatmAtaagcaaaamAtaataaaaamAtaataataaimAtaatgctaamAtaatggtcgiAtacaaaacamAtacaaaatamAtacagatacmAtacatatacmAtacattattmAtacgagtaamAtacgcaatamAtagaaaagamAtagaatggtmAtagacgaatmAtagctgttgmAtaggacttgmAtaggttatgmAtagtacatamAtagtagaagmAtataaaagtmzAgtttttataattggattga@Ataaatgtaa@Ataatgggaa@Atataagactttaataagta@|Atatattggt@Atattagtga@AtcaccaaaatAtgacgaaacatcaaacttt@}Atgcaaaccc@Atgtggtaca@tatgt@eAttttttaattatgctgtta@~Cagcaataataagatgttaa@Ccaaatgcaa@lCgtgataaagaagaaaagtt@Cttagtattatctaaatgac@Gaaaacggat@kGaagaaaatc@Gaattcctaagaaacaaata@Gaatttcttccactttttgt@TGatatatttcacatgttaaa@Gatgatattatgttaagaga@jGccaccataaattattatct@iGgagaagaat=AATATAAGACTTTAATAAGTAAAGGAATATCAGTGGGAAAATGTGTTAATTCACCATATGGATTATTATTAACCAAAAATCAAATATTAAAAAATATTCATGAATATAAAAAAGGTTACTTCGAAATTCAAGATGAAGCTAGCCAAATAGTGAGTTCCAAAATACCTGTACATCCAGGTGATAAGGTGCTGGATTATTGTGCAGGGTCAGGBTGGAAAAACATTAGCATTTTCTATGTTAATGGAAAATACAGGAAAAATATATTTACACGATATACGTGATCAAATGTTATCTCAAGCCAAAATAAGATTACGAAGGGCTGGAATACCAAATTATATTQ@A I293 25J12L17/04/96M17/04/96N61S48oC annealingTAnonymous marker[MiddleXB3a9f=3c10r(3c12A3c3f<3e3r;3f1fT3f6f24b4f'=KjHomology with Proliferation-associated nucleolar antigen Genbank protein acc. no. X555040001 December 1995L17/04/96M3/10/96N61P41R3D7 MunITony Triglia size selectedS48oC annealingTAnonymous markerU3D7B8V337 bpWcompleteXYesYFinal[Middle\150125^AC_51GTTATCTCAAGCCAAAATAAGATTACGAAGGGCTGGAATACCAAATTATATTQ@A I293 25J12P>A W 12-0059BAAGCGACTCGTTGTGTAAAAATG CCGTTCCATGTCTTCTCATCTAAT F267G6A4Fcc. no. X555040001 December 1995L17/04/96M17/04/96N61P31R3D7 MunITony Triglia size selectedS48oC annealingTAnonymous markerU3D7B8V337 bpWcompleteXYesYFinal[Middle\150125^DC_111>A W 12-0059BAAGCGACTCGTTGTGTAAAAATG CCGTTCCATGTCTTCTCATCTAAT F267G6A4FL22/04/96M22/04/96N62A45oC annealing@A>    X6a9f96b11fQ6b1f6b2f16b5fO6b9r/6c12fZ6d11f 6h2rC6h3f 7a11f57a12f7a2f7a4f87a6fL7c4f!7d4r*7d8r7d9f#7e12r:8a11r8a12f[8a9r\8b10f,8d5f]8d7f8e11fU8e6f8e7f 8f5fM8f8fD8g2r48g3f8g7rN8h4f0Abra6Atp2%Atp4Clat$Cpn@CspiCtrpmJtkrJt108vJt33gJt58jJt90OraPfuPf3.3fPoltRnatY130ls1imt10imt1021imt1081imt1141imtaaga>AAGATAGTTAGTAAGCTAGCTTTAGAAGCGACTCGTTGTGTAAAAATGGATAATTTAATGGGAAGAAAAGAAATTGATATAAAGAGATATGCAAAGGTTGAAAAAATTCCTGGAGGAGATATTACGGATAGTTATGTTCTAAAAGGTGTTATGATAAATAAGGACATTACCCATCCTAAGATGAGACGTTATATTAAGAATCCTCGAATATBTATTATTAGATTGTACTTTAGAATATAAGAAAGCAGAAAGTCAAACTAATGTAGAGATATTAGATGAGAAGACATGGAACGAATTATTATTACAAGAAGAAATAGAAGTAAAAAAAATGTGTGAATATATAATAGATAGT^@A I25J12>K\Homology with T-complex protein 1 gamma subunit Swiss protein acc. no. P39077 December 1995.L22/04/96M22/04/96N62P1RTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerUB8V350 bpWcompleteXYesYFinal[Middle\125^C_1?A W 12-0060BGCTACCTCTTTATTTCCATACAT CTATTTTCCACGATTTCTTTATTA F193G6A2FL22/04/96M22/04/96N63A43oC annealing@?AAGAAATACTTATAAGAGATACAAACGAAACAACTTCAATAAAAAATAATTTCTATTTTTCTCATGATTATTTATATATTCCTCAAGCTACCTCTTTATTTCCATACATATATGATTTATTAAAAAATAATAACAAACAGAATATACAAAAATGTAATGATACAATATATGTAAGAAATAATCCGTATAGTGAAAATACACCTAATAATAAByTATTGATGTAAATAATAATATAGAAAAAAAGCAAGATAATTATATTAATAAAGAAATCGTGGAAAATAATAATGTGCATATAATAAATAATTCAAACATATTAAATAAGCAACGAGCATC  K@A  I274 805 1383J12L22/04/96M22/04/96N63P3915R3D7 MunI3D7 MunI3D7 MunI?A43oC annealing@A>    >TAnonymous markerU3D73D73D7V330 bpWcompleteXYesYFinal[Middle\509590^GDD_1013@A W 12-0061BAGATAAGGTTTTTAGATTCTTCT CTAATTGTATCCGTCATTCATAGA DCPN 60F246GCPN 60I274 805 1383J12L22/04/96M22/04/96N64P3915R3D7 MunI3D7 MunI3D7 MunIS46oC annealingTKnown gene sequenceU3D73D73D7WcompleteXYesY Provisional1:`Taattgtatccgtcattcat@TactTacaatttttaattctccttTatkstuTataatcatagtgtgggtaa+@[Middle\509590^GDD_1013AA W 12-0062BGCTCTATCTTTAATAATCATATGTAAC CAACCATTTTAGATTCATCTTC F885G3C12I1383J12L22/04/96M22/04/96N65P15R3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D7WcompleteXYesY Provisional[Middle\90^D_3BA W 12-0063BTTTATCGTACGAAATGAATGAAG CATTTCTTCTCCGTTATCAATACC F171G5F11F/96M22/04/96N66A45oC annealing@BATTTCGATTTTTTATCGTACGAAATGAATGAAGAAAATGAAAATAATGATAATATGATATCTATATTAGAAAATATTCATTTAAGTTATTTATTAGGTGGTTGTAATATTATAGGTACTAATACGTTTCAAGTTAATTTATATAGTTTCAAAAAGTTAGGTATTGATAACGGAGAAGAAA @A I588J12L22/04/96M22/04/96N66P7R3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D7V179 bpWcompleteXYesYFinal[ Middle Right\100^A_12CA W 12-006422/04/96N67_ 03d7BCDEHIJKLMNOPQRSTUVWYZ[\]^_`abcfgijkmnqstuvwMuni  !"#$%'()*+-./0123456789:;<=?@ABCDEHIJKLMNOPQRSTUVWYZ[\]^_`abcfgijkmnqstuvw Ã4PÃ4<Ã45Ã5 GÃ5p@Ã5!Ã5 Ã60VÃ6.Ã7%Ã708Ã7PYÃ7>Ã8@#Ã9^ÃA=iÃC0ÃF0NÃGÃIp Ãd$ÃfIÃztÃfÃ6ÃÃ_Ã`A1019c`1033ca11t12.1I120r1a12rG1a8rY1b12f1c10r)1c3fW1d11r1e1f 1e9f1g11rE1h111h5r+1h7rH2a12_2a7f2c10r2c3f72e3rR2e4r2e7f 2e8r'2f2f.2f6f2g9r3.9u3/a12w3/a5q3/b4n3a8r CA  I588 614J12K[Homology with C. elegans cDNA yK45F10.3 Genbank protein acc. no. U 234120003 December 1995L22/04/96M22/04/96N67P77R3D7 MunITony Triglia size selectedA47 oC annealing@A>    >TAnonymous markerU3D7B8V405 bpWcompleteXYesYFinal[ Middle Right\100230^AD_122DA W 12-0065BCTTGTTTATAAATGTGATGGTTT CCCTTCTCATTCAGTTCAAAAATA DF163G8F8FATTTATGTGCGTGGTATTGTAAATATAAATATCGACTTGTTTATAAATGTGATGGTTTATTAATTGATTATAAAGGTACAATCAAAACGGACAAACCAATTTTCACATTAAAAAAAGAAGTAGGAATACAAAATAAAAAATCTATGATTGAATATATTTATAATGAAGACAAACATATTTTTGAACTGAATGAGAAGGAAGATATTTACAABAAAA@A I588 614J12L23/04/96M23/04/96N68P77R3D7 MunITony Triglia size selectedA44oC annealing@A>    >TAnonymous markerU3D7DB8V214WCOMPLETEXYesYFinal[ Middle Right\100230^AD_122EA W 12-0066BCTGTGGATAATTCTTGGTCCTTA CAATATAAATACGAAACATTGGAA F226G1G11RACTGTGGATAATTCTTGGTCCTTATGATATGCTATATGATTATATGTCGTTTCCTTAAAATGATTATCACTATTAATAACATTATCCTTTTTATCATCATCAACATCTATTAAACCACCCCCACCTTCCTCTAAATGGATTTGCTCCTTTGGGCTAGAATCATTATTATATACATTAAAATAATTACTATTTATTATGTTCTTATTCCAATBTGTTTCGTATTTATATTATGTTCTTTATCTCTTTTATGGTTATCATTTATTATTAGATTATTGGAATGATCAAGATTCATGGTGA&@A <}AnnealingEFGHIJKLMNOPQRSTUVWXYZ[\]^_`afgijknqrstuvwC/EOc.CAnonymous  EI588 614J12L23/04/96M23/04/96N69P77R3D7 MunITony Triglia size selectedA46o C annealing@A>    >TAnonymous markerU3D7B8V294WCOMPLETEXYesYFinal[ Middle Right\100230^AD_122FA W 12-0067BTTATTCAAGCGATGTAGGAAGTA CATAGATGTCGACAATGTTATTTG F231G4H7RL23/04/96M23/04/96N70A46oC annealing@ 7@A>    >TAnonymous markerU3D73D73D7V199 bpWcompleteXYesYFinal[Middle\6070150^CBA_1558A W 12-0053BAGCTCAATTAAGTAAGAAACATT CATAAAATTATTAAATCACAAAAC F262G7A4FAAGTTTTTATAATTGGATTGAAAAGTATGTTCGTTCACTTCATCAATAATGTGTATATTTGTATTATCTTCATATTCAAGAACTAAATTTGGGAGCTCAATTAAGTAAGAAACATTTTTGAATGTCGGAATATAAAAGTTAATAACATTTTCACCATATTTCAAAGTTTTGATTGCATAACAATTATATATATCCCATATTTTTATTCCATSkTtattcaagcgatgtaggaaFE FAAGATATTATTCAAGCGATGTAGGAAGTACACAGGTTTGTGCGAACCCAGGTGATGATCACAATAATAATAATAATAATAATATTAACAATAGTAATGGTAATGTTTCTAATATTAGTAAATGGAGAAATCGTTTTTTGTATAAATGTATGAATCTCTTTAGGAATGATAAAAAAAGCGTTTTATCTAATAAGTTATATAATTTATATGGAB5TCCCAAATAACATTGTCGACATCTATAAATTTATGTAATCCGAATTTATTTC  @A  I614J12L23/04/96M23/04/96N70P7RTony Triglia size selectedA46oC annealing@A>    > rFTAnonymous markerUB8V262WCOMPLETEXYesYFinal[ Middle Right\230^D_2GA W 12-0068BACAATTAGTGGAAATAAAACAAT CCACAGAAAGATTCATAAAGCATA F241G1A12RAAAAGGAAAATAATACAATTGGTGGAAATAATACAATTAGTGGAAATAAAACAATTAGTGGAAATAATATAATTAGTGAAAATAATACAACTAGCGAAAATATTACAACTAGCGAAAATATTACAACTAGCGAAAATATTACAACTAGCGAAAATATTACAACTGGCGAAAATATTACAACTGGCGAAAATAATATTATGAACGAACTAGC[NAcagtttggaaacataaataOAcatcatctagatcccacacAccagaacctactgttacta6AcgaaacatcaaacttttatActuActgcgcttaaataataatc AgaijAgaacattttatatacacatAgaagatgacggaagaggtaPAgaatgaagtattcttaataJAgacatgcatattcattctgAgagctaattctccaaagta-Agataaggtttttagattct@Agcaaggtaaaactccgctg    >TAnonymous markerUB8V420WCOMPLETEXYesYFinalG[ Middle Right\230^D_2HA W 12-0069BAAATAAATGTTGGTATGGTGGAG CTGTTACAACCTATTTTGCCTCAC F78G1H7RAAAATGTGTAACATGTATGATGACACTGCACATATATATATATTTTTTTATTTATTTATTTATTTATTTTTATTTTTTGAAGGTTCCGGAGGAGGCTAAACAAGACGAATATGGTATAACAAATAAATGTTGGTATGGTGGAGAAATAATGAACAGTTCAACTAAAAACAGAAAGGTGAGGCAAAATAGGTTGTAACATATAAAATGTTTABTAT @A I614 202J12L23/04/96M23/04/96N72P73RTony Triglia size selected3D7 MunIA45oC annealing@ HA>    >TAnonymous markerUB83D7V213WCOMPLETEXYesYFinal[ Middle Right\230140^DA_210IA W 12-0070BAGGTGTACACGTAACTGCTC CTCTACATTCATCTCATATTTGG F550G12.1A12.1F; CATTATCGTTTCCTTTTATGTGATCATTTGTTTCCACATGATCATTTGTTTCCACATGATCATTTGTTTCTACATGATCATTTGTTTCCACATGATCATTTGTTTCCACATGATCATTTGTTTCTATATGAGCACTTGCACATATGTCATCTTTTTCTTTTTCAAGACCATCCTCTTCTGAGCGATTATTTTCTTGTGAAAGA412.1fI12.1rI1201imt121imt1210i20m1230m1240m1250m1260m1261m1321m1381m1441m1501m1510m1520m1530m1540m1550m1560m1561m1621m1681m1741m1801m181m1810m1820m1830m1840m1850m1860m1861m1921m1981m20m2041m2101m2110m2120m2130m2140m2150m2160m2161m2221m2281m2341m2401m241m2410m2420m2430m2440m2450m2460m2461m2521m2581m2641m2701m~7FIJKLM]^_afgijnG?3FGV~\P‚!EJFkHlJmLNPRT`VXZ\^`bdfhjlnsFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALK@x^At@IWM@TBUBWT @jJi Ah IA>    >TAnonymous markerUB83D73D73D73D7WcompleteXYesYFinal[ Middle Right\230140140135145^DGAGF_221028JA W 12-0071BAGAATGAAGTATTCTTAATACAC CAACGTATATACATTCAAGTTCAG F73G5B12FAeAAAGATTATATATTAAGAATGAAGTATTCTTAATACACATCATGAATTGTATTAATATTCAATATTCTGAACTTGAATGTATATACGTTGACTCTACAAAAe@A I458 181 356 IAR  R W RI614 266 202 458 356J12K1. Y266 (Mun1 3G2) has been assigned to chromosomes 12 and 13. 2. Ordered primers based on F and R sequences where there was no detected overlap.L23/04/96M23/04/96N73P73354RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunI3D7 MunIA50oC annealing@rkerUB83D73D73D73D7[ Middle Right\230IBGCAGTTACGTGTACACCTTCGTTATGTTCATTATGTTCATTTTGTGGTAAGGTTTTTCTATTTTCCATTTTCCTTTCATTGAACAATTTAATAGCTTGAAGAATTAAAGGAACATAATCCAGACATA 12.1R; GATATATTTCACATGTTAAATATTTCAACATCTCCTCTACATTCATCTCATATTTGGTACTATTCTTTGATCGTCCGTTTTCTCCTCTTTTTATTTTCCCTTAACACTGTTATTGTCGTTTCAGTATAGTCATCAATGTACCTTTCTAATACGTCAACATCTTGCTTTTTATTTTCAGCTGATACAGAGTTCTTCATCAAATGTGGGAAACCGAAAAGGTTCTTGGGAGCTTCTCCTTGAGTATTATTATTCACTGAGTAACGACATTTe@JJ12KAY181 (Mun1 2H1) has been assigned to chromosomes 12 and 6 and 13.L23/04/96M23/04/96N74P524R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D7V101 bpWcompleteXYesYFinal[ Middle Right\135230145^GHF_218KA W 12-0072BATAAAAAATGAACAAGTTAACAG CTGATGGACGTCTAGGAATTACCG F95G5H11FI458 181 356 1386J12KK1. Y181 (Mun1 2H1) has been assigned to chromosomes 12 and 6 and 13 2. y1386(Mun1 15D6) has been assigned to chromosomes 12 and 13L23/04/96M23/04/96N75P52415R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7WcompleteXYesY Provisional[ Middle Right\135230145155^GHFD_2186LA W 12-0073 , AndKLMNOPQRSTafwAntigen=SArginyl!ArmAssignedIJKLMNOPQRSTaB8BasedIBe-./0BeenIJKLMNOPQRSTaBelieveBothfwButByCCCamp-dependent-Cdna)CMChain-:Chimeric-./0Chromosome- LBCTAAACAGTTGTGCTAATACATA CTCCTTGTTTAAACTCCTCATCAT F158G7A6FATGTTGTAAATATAAATAATTTCATAGAAGATCTAAACAGTTGTGCTAATACATATGATGCCTTATCAAAAACAAATGATATGTTTTTGAAAAAAATGAAAAGTGATAATAATAATAATAATAATAATAATAATAATATTATACAAAAAAATGAAGAGTTACAAATCATGATGAGAGATTTAAACAAGGAGGTTACACACAAAAA @A I181 356 1386J12K|1.Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 2. Y1386 (Mun1 15D6) has been assigned to chromosomes 12 and 13L30/04/96M01/05/96N76P2#MWcompleteXYesYFinal[ Middle Right\23015510045small^HDHDF_161141NA W 12-0075BGGAAGCAATATAATGAGTAATGA CTTAAATAATTTAAATGATGTTTT F352G8G7RAAGAAATAACTTTTTAGTTTCTATGAATACTATAAAAAATAATGGAAGCAATATAATGAGTAATGATAATAATATAAATAATCATAGTAATAATAGTAATGATAATAATCATCACATGGATAATAATTTAGAAGCACAAATAGAAAATAATACAGATATGATTATGGATAGTAATATGGATAATGATATTGATATGAATATAGATAAAAAGrU3D73D73D7[ Right Middle\23010045^HHD_11143D7[DOPSTUV[\]^_`fgE  !"#$%-STUVYZ]^_jmnqF%'()*+-./0122-0026W12-0027W12-0028W12-0029W12-0030 W12-0031!W12-0032"W12-0033# M 40 1117J12K1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 2. Y1386 (Mun1 15D6) has been assigned to chromosomes 12 and 13 3. Homology with P falciparum cDNA clone 0424C Genbank nucleic acid acc. no. T18056L30/04/96M01/05/96N77P2155112R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7L415R3D7 MunI3D7 MunI3D7 MunIS42oC annealingTAnonymous markerU3D73D73D7V204WcompleteXYesYFinal[ Middle Right\230145155^HFD_186MA W 12-0074BCAGAATCTTTTCCAGTTACTTAT CTATGAAGGTGGTTTCCATTTACT F106G8F5FATCAGGTATTTGGCCCTCCAGAATCTTTTCCAGTTACTTATAACGGATAAGAAACAATTGAATATTCAAGTGAAAATAAAAATATCAAAAAATCGTCAAGCAGTAAATGGAAACCACCTTCATAAATTATATTTTTAATTATGTACTATAAGGTTATATGAA@A I181 1386 47993L M WNB#A$OB'PK&QC)K%RA,+S@./T 01UKQVA4J6W@5@7V8XB=>YL@ZNA[ABLD\AE^F]G^AJK_TLaKMcYNfBUJg@MVVAhCEqGpIoYajUYk@Xnl@ZmCEGIKMOQSUWY[]_acegikmoqs`n@ o@^qq Ã!NOPQRTUVYZbckÃ"fgistÃ"`'/01Ã# ^_Ã#`./09:;<=>STfgjÃ$%'()*+nÃ$` IJKÃ%HIjÃ%`IJKLnÃ&789:;<=XYZ[Ã&`KLMiÃ'STUVmuÃ'`013gvw$NBAATATTATTAATAAACATAATAATCATGATGAAAATAATAAAGATATGAGAATTAATTTATGTAGAGATGCTATACTATTCATTTTACATGATCTTGAAACTATATTAGAATTAAATATACCTATGCTTAGTAAAAATGTTAATATGTATAAATTATGTATAAAACATCATTTAAATTATTTAACCTTAATAAAAAATGA @A I181 479 40J12K=1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 L01/05/96M01/05/96N78P251R3D7 MunI3D7 MunI3D7 MunIA43oC annealing@#' NA>    >TAnonymous markerU3D73D73D7V410WCOMPLETEXYesYFinal[ Right Middle\23010045^HHD_1114OA W 12-0076BACAGTTTGGAAACATAAATAAGT CAGTACCTTCTACACTCTCCTTTT F157G6B5FAAACAAAACAATTCTGCCTGCTCATCTGAATTATTCTTCCTTTGAAAATACACCTTCAAGCTCTTTTCATCCCCATCATTCAAAACCCTATAATCAGTACTCCCCATTTGTTTTTTTACATACTCTTAAAAAATATTATATGTACAAATATATACATATATACATACATACATACATATTATATTTATAAATACATATTTATTTCATAAAT),QK1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13. 2. Homology with AFG3 gene Genbank protein acc.no. X766430001 December 1995 L06/05/96M06/05/96N81P25R3D7 MunI3D7 MunIA48oC annealing@A>    >TAnonymous markerU3D73D7V312 bpWcompleteXYesYFinal[ Middle Right\230100^HH_111RA W 12-0079BAATTCTTGTGCTTCATCTAAATC CAAATCTTGTGCTTCATCTAAATC F89G2E3R1H)PK1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13. 2. Homology with DNA gyrase Genbank protein acc. no. X546310001 December 1995 L03/05/96M06/05/96N80P251R3D7 MunI3D7 MunI3D7 MunIA50oC annealing@A>    >TAnonymous markerU3D73D73D7V319 bpWcompleteXYesYFinal[ Middle Right\23010045^HHD_1114QA W 12-0078BGGTTGGAAAGAGAAGTTCAGGAT $OB4TAATTTTCCTTATTTCGTTATAATAATATTTATATTATTTATATACAACAAA@A I181 40 479J12KF1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13. L03/05/96M06/05/96N79P215R3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    >TAnonymous markerU3D73D73D7V262 bpWcompleteXYesYFinal[ Middle Right\23045100^HDH_14SGtatactggatctaacttct#Gtattaaattactttcgggt)GtgtgctattatgactcttaGttkGttatgagagatgaaaaaga.Taaagcttatattaaatggt+Taagtatttgttttattatt Taatgattctaatataaaga9TacfrTagatgtgatggtgtatgtg TatgTattttctattttttatgtg3TcakTcaaagtcctcagtgctaac\TcatataagatgtgtaggagTccfTccattattactttcttgtg7Tctaaatgactctcttcaga1TctatactatctaagcataaTcttattatcctttttattt TcttccttaagtgatgaaaaTgarsTgattagcacaatcaatagcTgcctctattgtgtatacatTggtgttgatacaaa,&%QCAAAGCACTGTCTAAGGTATCAAT F159G6B11FATAAAAGAATACGGAGTTATTTGTTCATGAAAGATCAGTTGTTCCATCCATTGTATTTATTGATGAAATTGATGAAATTGGTGGAAAGAGAAGTTCAGGATCTGTTAACGGTGCAGGTCAAAGAGAACATGATCAAACATTAAATCAATTGTTAGTTGAAATGGATGGATTTAGTAATACTGTACATATTATGGTTATAGGTGCGACAAACBfAGAATTGATACCTTAGACAGTGCTTTATTACGACCAGGTAGATTTGATAGGATCGTTTATGTACCATTACCCGATATTAATGGAAGAAAGAAAATTCTTTTG8@A I181 479J122pt X766430001QY1103Y1160STUVY1386KLMY181JKLMNOPQRSTY266IY294mY461)*Y69a,. R>TAnonymous markerU3D73D7V203 bpWcompleteXYesYFinal[ Middle Right\230100^HH_111SA w 12-0080BATGGCTTTCTATACTGTTTCTCG CAAAAAAAATTAGATGATAGTTTG F156G5B1FAGGTGCTCGGTACATGTCATGGCTTTCTATACTGTTTCTCGATTCGCAGTCCACCAATCTTCTCGTAATTGAAAGAAATCTCCACCGTCATTTGTGTAGCGTTCTTTTACACCACCTGTCAATCCTTCATATATTTTCCCGAAAATTGTTTTCAAACTATCATCTAATTTTTTTTTTTTAAACTTATTAAAAATGTTTTGATTTTTTCCTTBMTTTTAATTTAAACTTTTTTTTAAAAATTTCGTGCAAAAATAGTAAAAATTTTTGAAAAATAATCTTTATTTTTTGT %+RAAATTCAATATTTATATTATTTAATTCTTCATTTTTTTCTTTTAAATCTAATATTAATTCTTGTGCTTCATCTAAATCGATTTTCAATTTTTTAATATCTTCTCCATCATCAATCATTTTTTCAATAGATTTTCTGGTAAGTTTTAATTTTTCTTTATTCATTTCTTTTATTTCTTTTTCATAATCATTAATAATTGAATTCAG @A I181 479J12KD1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13.L06/05/96M06/05/96N82P25R3D7 MunI3D7 MunIA42oC annealing@A>    K i ÃDpÃE  ÃEÃFpIJKLÃFÃRP !"#ÃSVWX`ÃTPjnÃU YZÃVIJKÃW )*ÃW0ÃWPÃX`345ÃXMNOPQRÃZ0 ÃZ`%'(Ãa 'ÃdPTUVÃd !"#Ãe./012ÃeijnÃfPgiÃhXYZ[Ãi0+,-.aÃiBCDEbcÃj0uÃjP$%Ãq@01ÃrPCDEF+/S@A I181 1401 336 1160J12K1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13. 2. Y1160 (Mun1 13A8) is a multi-transformant. 3. Homology withVariant-specific surface antigen 7 (var-7) Genbank protein acc. no. L426360001 December 1995.L06/05/96M06/05/96N83P215413R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    .0 S>TAnonymous markerU3D73D73D73D7V286 bpWcompleteXYesYFinal[Right\230125160^HEDA_19128TA w 12-0081BATTATATTTAAAGTTTTCAGAGC CTATCTATATTTTCCCCCTCCTCA F145G3F1FAACATTATAAGTGATTATATTTAAAGTTTTCAGAGCTGGTTCCATAGGAGATGGCTAATCGAGTATATATATTAAATGGATTATAGGAAAGAAAATAACCCCCAGAAATAATGAATGTAATTATAATATCTATATTGAGGAGGGGGAAAATATAGATAATAAAAATGAAAATTTAAATGAATATTATATAG @A I1401 336 534D77/1T 996 1160J12Kt1. Y181 (Mun1 2H1) has been assigned to chromosomes 6 and 12 and 13. 2. Y1160 (Mun1 13A8) is a multi-transformant. L06/05/96M06/05/96N84P15461113R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V190 bpWcompleteXYesYFinal[Right\125160100100^EDECA_9120QyT6128UA w 12-0082BTTTGTCCTGCTAGAAATAGACCT CTCAATAAAAGGATTAGAAAATGC F133G8E11FATGTATTTATTCCTGCAGCAATTCCTTGACAAGCTGCTTCTTCATATCCTGTAGTACCACAGATTTGTCCTGCTAGAAATAGACCTTTAACATTTTTTGTTTCTAAGGTATAATTTAAACATTTAGGATTAACATAATAATATTCAACATCATAGGCAGGAAAAACGATCTGTGCATTTTCTAATCCTTTTATTGAA@A I336 534 996 1160J12 markerU3D73D73D73D7[Right\160100100^DEC_12612C* X625400006U@ABCDEFGHIJKLMNOPQRSTRCompleteUVWXYZ[\]^`bcNoYes  !"#$%'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^`bcfijkmnqrswQ6VA W 12-0083BAAATATGAATATGGATACTATAC CTCAAATGCATCTTTATTTTCATG F252G5B11FAGCTCTGATCCTTTAGAAAATATAAAAAATATGAATATGGATACTATACAAAGCCCCCGTCTCAAAAATGATAAAACATATAATAATAGTAATAATAATAATAATAATAATAATAATAACAGCAGTTGTTTTTATAATAATTCATATCATAATAATTATATTTATAGAATGAAAAAATTAGAAAAAGAAAACTTTTTTCTAAAACATAATCByAACCTTTAATCAATCAAATTGAAAATCAATTAAACACAAAAAATCATGATAATAAAGATGCATTTGAAAATGCATGGATAAAATTAAATGAAATTAATAAAGAAAATGAACAAGGATCTA K@A I336 534 1160 312 42067JWA W 12-0084BCAATATTTGAATGTAATGTCC CCGTTAAGGAATATACTCATAA F147G1C3F6P4645R3D7 MunI3D7 MunI3D7 MunI3D7 MunITony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7B8V330 bpWcompleteXYesYFinal[Right\16010035180^DEBC_1261212WA W 12-0084BCAATATTTGAATGTAATGTCC CCGTTAAGGAATATACTCATAA F147G1C3F645VJ12K-1. Y1160 (Mun1 13A8) is a multi-transformant.L06/05/96M3/10/96N86P461345R3D7 MunI3D7 MunI3D7 MunI3D7 MunITony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7B8V330 bpWcompleteXYesYFinal[Right\16010035180^DEABC_12681212I336 534 1160 312 420J1258WACTCCTTAATCCAATATTTGAATGTAATGTCCCTTATTTTTTTCGCATTTTGTCTTATCGTTTCTTTTAATTTTTTATTGTTATAATTATTAGATGATCTTATTTTAATATGATTACTTATGTCAATGTCAATAATATTATGAGTATATTCCTTAACGTCCATACTATTTATATTCTTACATGGTATTATACAATAGTTTCATTTTTTTTCBTCAA @A I312 420J12L07/05/96M07/05/96N87P45R3D7 MunITony Triglia size selectedA41oC annealing@A>    >TAnonymous markerU3D7B87=hWV214 bpWcompleteXYesYFinal[Right\35180^BC_1212XA W 12-0085BAATATTTTCTGATATTTCATCAG CTAATCATATTATTGATTATACAG F79G5H1FATTTATCACCTGAACTTGTAATATTTTCTGATATTTCATCAGATTTATTCTTTTTATCGTTATCCATTTTATTATCTGTATAATCAATAATATGATTATTTTGTAATTTATTACCATCTCGATCATTTTGTAATTTACCACTAACTTTATCATTTTGTAATTTACCACTATCTTTATCATTTTGTAATTTACCACTAACTTTATCATTTTGmarkerUB8B8[Right\180150!01@AA2AA*3@A4@AA5BT6\7@ 8B[9@B[m:K;AT<A=AK>@AK?AA@[BACBADFEFA T GB[HAIBAAJJKKLBITaatcatattattgattataXEFGHIJKLMNOPQRSTUVWTaattgtatccgtcattcat@Tatactttccatgcttctct@Tccctatagatgattcttgt@_TctttttcttcttcctctatTttttccatgttatttattg@Ã"@Ã4P3a9f@4b4fFX6a9f@12.1f@1210m15012710@Aaaagtttataaaaatagat@rAaagaaattgaaaaaataat@sAaatgtaaacacattaggaa@cAacatttaatatttttttat@tAatatctttataacattttg@uAatgcatcatttcataatat@Aatttttaaaaatactcatg@dAcaatcttttttataacatc@vAcatcatctaataatttcat@wActtcgtgttttaaattttt@y  45ocXYZ_`agijknqrstuw46oE46oc )*;@F47.C47oc'0G48oc!<=Q49oc50ocIPAnnealing  !"#$%'()*+,-./0123456789:;<=>?@ABCD8>XBTAATTTATCACTATCTTTATCATTTTGTAATTTAACACTATCTTTATCATTTTGTAATTTAACACTATCTTTATCATTTTGTAATTTAACACTATCTTTTTCATTTTGTAATATATCACTATCTTTATCATTTTGTAATATATCACTATCTTTATCATCTTG u@A I420 579J12L07/05/96M07/05/96N88P57RTony Triglia size selectedTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerUB8B8V372 bpWcompleteXYesYFinal[Right\180=@X150^CA_123YA W 12-0086BGAAGATAACACTGACGGGAACAG CTTTCAATAATTACATTTCTGGAA F264G1A8RACCCACCCATGTTGATTCCTCTGTATGATTCGGAAGATAACACTGACGGGAACAGTACAGGTAAATATAAACATAAATATAAACATAAACATAAATATAAATATAAACATAAATATAAATGTGGGTATATATATGTATAAATGTGGGTATATATATGTATAAATGTGGGTATATATGTTTATATGTTTATGTACATATGACAATAATTGTABrACGATGTAAATATATTCAATCATTTTTCTTTCCATTTAaTCATATTTATATCCACACATGTTTTCCAGAAATGTAATTATTGAAAAGTATATTATTTTATTATTTTATTAATTTD@A I579 441J12J CGPRIMER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()BR]@\ALK@x^At@IWM@TBUBWT @jJi Ah Ah>AYL07/05/96M07/05/96N89P75RTony Triglia size selected3D7 MunIS45oC annealingTAnonymous markerUB83D7V324 bpWcompleteXYesYFinal[Right\150100^AE_39ZA W 12-0087BAAAATACTTATTAGGTTCCTGTG CAATTTTAGATGCTTATCCAATAG F117G6C12FAAAAATATTTAAAAAATTGAAATTTCCTTTTAAAAAAATACTTATTAGGTTCCTGTGGAAAAAAAACTTTGTTGTTATAATTGCTATCCTTCATGTATGGTTTCGGTGGAGGATTAATTGTATTTTGTACTATTGGATAAGCATCTAAAA@A I579 441J12L07/05/96M07/05/96@BNZN90P75RTony Triglia size selected3D7 MunIA45oC annealing@A>    >TAnonymous markerUB83D7V149 bpWcompleteXYesYFinal[Right\150100^AE_39[AW12-0088BATATCTTCAGATACTGGAACTAT CAATAAATTATCTAACAAACAATA F96G8A12F9 614J12L07/05/96M07/05/96N91RTony Triglia size selectedA43oC annealing@A>    AD[AAATTCTTTAATATTATAAACAAAAATTAAATGATCATCATAGTCATATATTTTTTGATCAAAAGTCATAACAAAATGTCTTAAGGTATTATTTATTACAGCTGTATTATCTCTTACCCATGTTATGTAATCATATATATCTTCAGATACTGGAACTATTCTAGGAGCAGGCATAAGATCCCTAATAACATCTGGTACGGTTTCATTAGTABoTTGTTTGTTAGATAATTTATTTGTTCTAGTGCAATATTTTTTTTTATATCTACTTCTAATATATGACCTTTTAGTTTGTTTATTTCGAGATCTAGATCTTTTAAACTTTTGA@A I579 614J12Kb1. Homology with P. falciparum sexual stage ORF Genbank protein acc. no. M691470001` December 1995v2  SexualSignalStage[Stock)*StsStss-./0Subunit>SurfaceSSwiss!-.:>Synthase!T-complex>T02521)T18056MThe-./0ThereIThisThree-./0To-./0IJKLMNOPQRSTaTonyTrigliaTrna!TwouUCU194900001Var-3Var-7SVectorWasIWeWhereIWith!)-.:=>CMPQU[Withvariant-specificSWould)*X546310001PX555040001=BEb[L07/05/96M07/05/96N91P77RTony Triglia size selected3D7 MunIA43oC annealing@A>    >TAnonymous markerUB83D7V321 bpWcompleteXYesYFinal[Right\15075^AD_32\A W 12-0089BTCAAAGTCCTCAGTGCTAACCAC CAATTTATATTATTCTCATTGGAA F136G8A9RL07/05/96M07/05/96N92S43oC annealingTAnonymous marker[RightDF\ATAAAAATATTATTATTATTTCTTTTGTTAATAAAAATATAACTCTTTCTTATTTTAAAATAATTTATAATATATATTTTTTATTATATATTAATTTTTATAAAAGGTCTCGCCGGGGACTCGAACCCGGGTTGGAGGATTCAAAGTCCTCAGTGCTAACCACTACACTACGAAACCTTATGCACATAATTTTATATAATTTATTATTAATBjAAGATATTTAAATACGGAAAGAGAAAAAATATGTTTTAAATATTCCAATGAGAATAATATAAATTATATTTTTTACATTTATATTACTATAATATACATTTATTT <@A I329J12L07/05/96M07/05/96N92P4R3D7 MunIS43oC annealingTAnonymous markerU3D7V315 bpWcompleteXYesYFinal[Right\85EG\^D_5]A W 12-0090BATATTATGCTTCAAAGATTCTAT CAATACATATGATGATACATTTTT F194G8D5FATAAAATCTTTTAACCTTTCTAAATTAGGTATCACCTTCTTAATTTTATTTTCTATATTATGCTTCAAAGATTCTATTAATGATATAATATGATCAATATGGTCTATATAAGGTTTAATTATTAAGGTATCTATATTTGGTTGTAATATATTATAATTTACATTTAAACCATTTAATTCAATTTTGAGAACTTCCCATTCATAATTATCTCBICTATTAATTTCTCTAAAAATGTATCATCATATGTATTTATATTTTCCTTTAATTTCTTATATTTCTCATTTT @A I329 156 1224J12L07/05/96M07/05/96N93P4213R3D7 MunIFJK]3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D73D7V282 bpWcompleteXYesYFinal[Right\857575^DEF_51212^A W 12-0091BCTTATTAAAAATTGATGAGGATA CGGATGATTAAATTTGTTGATTAT F289G5E8F9 156 1224 25J12L07/05/96M07/05/96N94P4213R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@ker[Right12-0091^12-0092_12-0093`12-0094a12-0095b12-0096c3-0003g3-0004i3-0005n3-0006j3-0007k3-00083-0009q3-0010m3-0011r3-0012s3-0013t3-0014u3-0016w:Ggatgattaaatttgttgat^GtjGtacatgtacagatcttagawGtaggtagctttttgattca7Gtagtaataaaacgaaaagt/GtckGttgkrGtttgatcggtgatagaatg    >TAnonymous markerU3D73D73D73D7V341 bpWcompleteXYesYFinal[Right\857575120^DEFC_512121_A W 12-0092BAATATAATAGAGAACATCTTC CTTAGTTATACAGACATTCTAG F810G2A12I25 1116 1193 1320J12L07/05/96M07/05/96N95P1121314R3D7 MunI3D7 MunI3D7 MunI3D7 MunIS45oC annealingKM_TAnonymous markerU3D73D73D73D7[Right\120small75small^CEDF_112512`A W 12-0093BGAATATAAATCGTGCTTACCCCCATTTGTTTGTACTACGTTCAGGD1019CF~220G1019cI420 614J12L07/05/96M07/05/96N96P57RTony Triglia size selected3D7 MunIS45oC annealingT cDNA cloneUB83D7V387 bpWcompleteXYesYFinal[Right\18075^CD_122aA w 12-0094BCTACTATTCTTTCTAACTCTGCCCAGGCGAAAGCTATAGTTCTACD1033cF~210G1033cI582 69J12 & 5LNaK:Y69 (Mun1 1F9) has been assigned to chromosomes 12 and 14.L07/05/96M07/05/96N97P71RTony Triglia size selected3D7 MunIS45oC annealingT cDNA cloneUB83D7V298 bp[Middle\370175^AF_69bA W 12-0095D 5 K1cl12G5K1cl12I588J12L07/05/96M07/05/96N98P7R3D7 MunITKnown gene sequenceU3D7WCOMPLETEXYesY Provisional[ Middle Right\100^A_12cA W 12-0096D3K1cl12I588J12L07/05/96M07/05/96N99P7R3D7 MunITKnown gene sequenceU3D7WCOMPLETEXYesMUcY Provisional[ Middle Right\100^A_12dL26/9/96M26/9/96N100eL3/10/96M3/10/96N101fA W3 - 0002BCCA TGG AAA TCC ATT TAC CAA C CCGA AAG GCT GAT GGA GAC TCCF700GpF3.3AGAATTCCTAAGAAACAAATAAGAATATATTATATACGAGCAATATAAAGACATGCAAATATATACATATTATATATATATATATATTTATATATACATATATACCTTTAGTGATATTAACAAATCTCGAACGAAAGATCGAAAGGCTGATGGAGACTCCACACAAAAATTAAACATATCGACGGAGAATGTCTCTATGTGTGTGGGGGTTD73D73D7[Left85kbP0K1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. YAC 548 (Mun1 6F8) is a multitransformant 3. One more YAC:3D7 Mun1 9D8 YAC 812 (185 kb)L31/03/96M07/05/96N48P1419167913R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA47oC annealing@A>    O 0>TAnonymous markerU3D73D73D73D73D73D73D73D7V257 bpWcompleteXYesY Provisional[Middle\401751251158570165^BFBHFCBD_69107838814UK1. Y1160 (Mun1 13A8) is a multi-transformant. 2. Homology with Glucose inhibited division protein A Genbank protein acc. no. X625400006 December 1995 L06/05/96M3/10/96N85P461113R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V196 bpWcompleteXYesYFinal[Right\160100100^DECA_126128V)]BTCTATACTATCTAAGCATAATCACATAATTATTTTAACCAAGGAF150 bpGJT90ATTATGTCTTCCATTTATATTAGTTTTTATTTTTATTTTTATTTTGGTGTTTTTTTTATTTCCTTCCTTTATAGTATCTATACTATCTAAGCATAATAACAATGTGGTTAATATACGACTATGATCATTATTTGGATTAGGAGATGAAGAAGGAGATAAGGGCACATTATTTCTTTCCTCCTCATCATTTATAATGTTAATATATCCTTGGB9TTAAAATAATTATGTTTATCATGATCATTGCCATTTATTAATTGATTCTGAACATTT  I257 810 124 301 462J3L29/11/95M17/1/97P392457R3D7 MunI3D7 MunITony Triglia size selected N8h4f0( `TggfTggagaaaagaaattaggaaTggtctcttcaaattaga$TtaijstuTtaaatcatgttgatacaaa,Gaatttcttccactttttgt GacaacgagaiGacaatgatgmGacaatgggamGaccaaatattGactgtccatmGagaaaacgcmGagaataaatmGagaatatccmGagacagtaatGagaggaatctGagagtaaaatGagatgaagamGagcaaaatatGagcctttggmGaggaatggamGaggtagtggmGagtactaagmGagtatgaaatGagtattaaamGagtgattggmGagtgtgatamGagttaggtgtGataaiGataaacaaatGataactcagtGataatataatGataatatagmGataatgatatGataatgcagiGataatggtatGataatttcctGatagctgctmGatataaatgmGatatacatamNfBAAATTCACAAAAGATTGAGTTAAAAAGTTGTTATTATATGTGGTTGTGGTATTTCTACTTCAGGTATATTTACTACTACAAATTCTAACAATCCGT........GAATTCTTACCTCTGATCGAAACTACCATAGAGAGAAGTTGTTATAATATACTAGATTTAGAAAAGGAATTGTTAAAAAGAGAGCAAAAAAAATTAAAAAATGAAACTTGATGAATCCATGGAAATCCATTTACCAACAATCGAAGAAGCAAAACAAATAAATTTTCAAATGACTAGTAATATATTAAATGTATTATTAGAAACTATATTAGTTGATTATAGAGATAGTAATCCACATATAAAAGATGCAGAAGTATTTTCATTATTATCAACAGTCTTTAAAAAAATCGAAAATGTAACATGTCCTATCTTACCTAD!CAGTACTAAATTATGTATTATTACAACAATAGAI42 114 170 1412W gM17/1/97N103P12615R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7[Left\125 kb165110^DGF_628onal[Left85kbBG62gAW 3-0002BGAG TAT GAC AAT ATT GAA CACCTA TCA ATT GTT CAG TTC CTTF150GJT33 554 1412J3L30/10/96M30/10/96N103P12615R3D7 MunI3D7 MunIVoAhL31/10/96M31/10/96N104iAW 3-0004BATG ATG AGA AAA TTA GCTCATG ATG AGA AAA TTA GCTDCSPF300GCSPA 10 20 30 40 50 60 1 ATGATGAGAA AATTAGCTAT TTTATCTGTT TCTTCCTTTT TATTTGTTGA GGCCTTATTC 61 CAGGAATACC AGTGCTATGG AAGTTCGTCA AACACAAGGG TTCTAAATGA ATTP9 554 1412J3Ksequence from malaria databaseL31/10/96M20/11/96N105P6615R3D7 MunI3D7 MunI3D7 MunIS45oC annealingTKnown gene sequenceU3D73D7aYjU3D73D73D73D73D7V168 bpXYesY Provisional[ Left Middle\80 kb55 kb125 kb230 kb140 kb^HFEFH_52296TCCCAAAACGTCAGATATTAAAAAATTCTGTGATCAGAATTTTGAAGATTACGAACTTATTTGCTTTGGTGAAACTGATGCCTTATCAGGAAATI154 328 300J3L8/11/96M20/11/96N107P244R3D7 MunI3D7 MunI3D7 MunIA45oC annealingATCAGGAAATI154 328 300J3L8/11/96M20/11/96N107P244R3D7 MunI3D7 MunI3D7 MunIA45oC annealingkbXnKkA W 3-0007B&CCT GAT AAG GCA TCA GTT TCA CCA AAG C CGTT TAT GAA GCT GTC AGA GAA AC F150GJT 55HTAAAAAAAATATTATTGTTTATGAAGCTGTCAGAGAAACTGCAGGAAACTATTATAGTGATCAAAGATCCCAAAACGTCAGATATTAAAAAATTCTGTGATCAGAATTTTGAAGATTACGAACTTATTTGCTTTGGTGAAACTGATGCCTTATCAGGAAATI154 328 300J3L8/11/96M17/1/97N107P244R3D7 MunIAAAGATCCCAAAACGTCAGATATTAAAAAATTCTGTGATCAGAATTTTGAAGATTACGAACTTATTTGCTTTGGTGAAACTGATGCCTTATCAGGAAATI154 328 300J3L8/11/96M17/1/97N107P244R3D7 MunInlL13/11/96M13/11/96N108mAW 3-0010DCTRPGCTRPA 10 20 30 40 50 60 1 TATATTATTA AATTAAAATA TGTTAAGGAT TTAATTTTTT ATAAGATAAT GGAAATTATG 61 AAATTGTCAT AAATTATTTC CTAGTTAGCC ATAGAATGGT TGATTAGGCA GCBCTTTTCAA 121 AAAAAAAAAA AAAAACAACA AAAAAAAACC ACCATAAAAT AAAATAAATA AAAATAATAA 181 TAAATTAATA AAGACATTTA TACACCATTT AAAATAAATT ATTATATTTA TTATTTTTAT 241 AATTGTTATA TTTTATTTTT ATCCTTTTTT TTTGTTTTTT TTTTA GC3D7B8IA +,-.789:;<=BCDEHISTUVXYZ[abcmuvwB $%'()*./01567VWfmrsuvC  !"#$+-01345679:;<=>TUVWX^_`stuD !"#$()*+013456?@ACDEFGHIKLMN]LTony Triglia size selectedTony Triglia size selectedTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D73D7B8B8B8B8V267 bp[Middle\125 kb65 kb^FDCBG_56416R\`Tony Triglia size selectedTony Triglia size selectedTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D73D7B8B8B8B8V267 bpXYesY Provisional[Middle\125 kb65 kb^FDCBG_56416 810 124 301 462J3L29/11/95M20/11/96P392457R3D7 MunI3D7 MunITony Triglia size selectedoL20/11/96M20/11/96N111pL20/11/96M20/11/96N112qAW 3-0009BGCGCACAAAAGAAAATCCTACCCAATTTTAAATATTTCAGAAGF120 bpG3/A5HTGAAGGAATGAATTGTTTATACTAATTTTAAAAATATTCAGTATAATGAAAAAAACTGTAAAAATATATTTTCTTTTTATATTGCGCACAAAAGAAAATCCTACTATATATGTAAATTGATATTTGGATATAATTTTTTATAATTTACATGAATTATTATATATGAAAATAAATTAATTCAGTCTTCTGAAATATATAAAATTGATTAAAAGATTAAGGAATAATAATAAAAAAAAAAAAAAAAAACCTI1109J3L21/11/96M17/1/97N113P12R3D7 MunIS45oC annealingTAnonymous markerU3D7V249 bpXYesY Provisional[Middle34 56789:;<=IJKLSTUVW\]^kmr5 !"#)*345IJKMNOPQRVWXYZ`jn6  !"#%'(./012TUVgijn7 !"#$%+,-.01BCDEFGHIXYZ[`abcru8 !"#$%9 !"#$%./013456?@3d7  !"#$%'()*+-./0123456789:;<=?@AmETCA CACGTTTTTA GAAACTGTCT TTTATAACAC 610 620 630 640 650 660 601 ATTACTCTTG TTTCTCTACC TGAAATATTT GTTATTATTA TTGTCAAATT CTGTTATATG 661 TTTTACAATA TTTTAACTFTT ATCTATTTTA AAGATTCAGT TAAAAAAAAA AAATATATTC 721 ATACATATAC ATATCTATTT CATAATCTAA AAAATTAAAC AAATAACAAA TGAAGAAAGC 781 ATTTGTATTA ATATTTTCCT GTTTCTTACT TTTTTTGCAT TTGCACATTG TAAGAACGCA 841 CTATGCAAAA3D7B8XYesY Provisional[Middle95 kb160 kb^AEhXjAW 3-0006BATG ATG AGA AAA TTA GCTCCCA TAA ATT AAT TGA GTF110GJT58HTTGGGTGAGTTAGTTGTTAATGCTGTTTATAAATTAGGTGATAATTTAGATAAATCTAATATAGGTATAAAAAAAGTTACAGGAGGTTCTTGTTTAGATACTCAATTAATTTATGGTGTTGCATTTAAAAAAACATTTTCTTATGCTGGTTTGGAACAACAACCAAAAI185 254 434 549 1242J3L31/10/96M17/1/97N106P235613R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIS45oC annealingTAnonymous marker7 MunI3D7 MunI3D7 MunI3D7 MunIS45oC annealingTAnonymous markerirAaaaaagaatattgaaagta7AaaaaataactAaaaaataattAaaaaattaaiAaaaacaacamAaaaaccaaamAaaaacgattmAaaaagataatAaaaagggacmAaaaagtttcmAaaaataaaatAaaaataaattAaaaatcaaatAaaaatgacgtAaaaatgcagtAaaaattaactAaaaattacgmAaaaattagtmAaaacgaaagtAaaacgattatAaaacggaatmAaaagaataamAaaagatatatAaaagatttttattggtaccuAaaagggatgmAaaagggattmAaagagttaaattagttataAaagattatatattaagaatJAaaggaaaataatacaattgGAaataacgaaiAaatagtagaiAaatccaaatitAaatgtaaacacattaggaa AaatgtagatiAaatgtcaaamAaatgtgtaacatgtatgatHAaattaagagtAaattataaamAaattatttcmAaattcatattAaattcattttAaattgtcatmAaatttaattmAaattttcactAacaaaaaaatAacaaaacaattctgcctgcOAacaaagagamAacaaattacmAacaacaaaatAacaagagtgtAacaagcacatAacaatatattAacaatattamAacaatattgmAacaatgttttAacaattgtcmAacaatttcttAacacaagggiAacacctgattAacacctggamAacatatttgtAacatggaagmAacattaattmaatatgatgaagaAatgatattgivAatttttaaaaatactcatgAcaaaaatatmAcaaaaatgaaataacctatvAcaaagaagatAcaaccagtatAcaactattttAcaagaaatatAcaagaagtamAcaagatatamAcaagattcctAcaaggaatttAcaaggtaattAcaagtgtaamAcaatattcamAcaatcaaggiccciActtcgtgttttaaattttt!AgaaataactttttagtttcNAgaaatacttataagagata?AgaatattgagtttattaaaAgatagttagtaagctagct>AgatattattcaagcgatgtFAgatccaaatiAgattatgaaiAgcaaccaggiAgcaaggtaaaactccgctg    >TAnonymous markerU3D73D73D7V161 bpXYesY Provisional[Middle\85 kb100 kb70 kb^541_10412541mm541mm541mm541mm541mm360i360ii360ii360ii360ii360ii360ii60ii360ii360ii360ii360ii360iimmmmmmmmWpiBAAATTAT 121 GATAATGCAG GCATCAATTT ATATAATGAA TTAGAAATGA ATTATTATGG GAAACAGGAA 181 AATTGGTATA GTCTTAAAAA AAATAGTAGA TCACTTGGAG AAAATGATGA TGGAAATAAT 241 AATAATGGAG ATAATGGTCG TGAAGGTAAA GATGAAGATA AAAGACGATGG AAATAACGAA 310 320 330 340 350 360 301 GACAACGAGA AATTAAGGAA ACCAAAACAT AAAAAATTAA AGCAACCAGG GGATGGTAAT 361 CCTGATCCAA ATGCAAACCC AAATGTAGAT CCCAATMunIS45oC annealingTKnown gene sequenceU3D73D73D7XYesioqiDGCCA ACCCAAATGT AGATCCAAAT 421 GCAAACCCAA ATGCAAACCC AAATGCAAAC CCAAATGCAA ACCCAAATGC AAACCCAAAT 481 GCAAACCCAA ATGCAAACCC AAATGCAAAC CCAAATGCAA ACCCAAATGC AAACCCAAAT 541 GCAAACCCAA ATGCAAACCC AAATGCAAEAC CCAAATGCAA ACCCAAATGC AAACCCAAAC 610 620 630 640 650 660 601 GTAGATCCTA ATGCAAATCC AAATGCAAAC CCAAATGCAA ACCCTAACGC AAACCCCAAT 661 GCAAATCCTA ATGCAAATC901i910i920i930i940i950i960i961iAaaaaaaaatipiFC TAATGCAAAT CCTAATGCCA ATCCAAATGC AAATCCAAAT 721 GCAAACCCAA ACGCAAACCC CAATGCAAAT CCTAATGCAA ATCCTAATGC AAATCCTAAT 781 GCCAATCCAA ATGCAAATCC AAATGCAAAC CCAAACGCAA ACCCCAATGC AAATCCTAAT 841 GCCAATCCAA GATGCAAATCC AAATGCAAAC CCAAATGCAA ACCCAAATGC AAACCCAAAT 910 920 930 940 950 960 901 GCAAACCCAA ATGCAAACCC CAATGCAAAT CCTAATAAAA ACAATCAAGG TAATGGACAA 961 GbAaaagtttataaaaatagat AaaataaatamAaaataaatgmAaaataaattmAaaataataamAaaatacaaaiAaaatacgaccacacaaatg4AaaatatatttAaaatatgtamAaaatattaatAaaatattcgtAaaatattgctAaaatatttaaaaaattgaaZAaaatcacattAaaatcattatAaaatcctaamAaaatgatgaiAaaatgctaaiAaaatgtgaamAaaatgtggamAaaattaaacmAaaattagaatAaaattagcttAaaattattamtAaaattattttAaacaaaaaatAaacagtttgmAaaccagaacmAaacccaaaciAaacccaaatiAaaccccaatiAaactaatatmAaagaaaatgm AaagaaattgaaaaaataatAaagaatgaatAaagagatggiAaagagattatAaagagttaaattagttataAaagatataamAaagatgagcmAaagattaaatAaagattatatattaagaatJAaagctaaagtAaaggaaaataatacaattgGAaaggaaatamAaaggatgatmAaaggattagtAaaggattattAaagggttattAaagtacatcmAaagtttttcmAaataacaaamAaataacgaaiAaataagagamAaatacatcgmAaatagtagaiAaatatattcmAaatatgtcamAaatattatamAaatccaaatiAaatcctaatiAaatgatacamAaatgatcccmAaatgcaaaciAaatgggaaamcm Aacatttaatatttttttat0AacattttgttAaccacacgatAaccatttggtAacccctccgmAacccttgtgmAacctcaccatAacgagtaaamAacgataatttAacgatatttmAacggtggtgmAacgtaatgtmAacgttttaatAactaaaatamAactagatctmAactatttgtmAactcagatgtAactgaatggiAagagggatgmAagatatatgmAagatatcaamAagattcagtmAagcataggamAaggaccatamAaggatatagmAagtaaatatmAagtataactmAagtcatatamAagtgaaacamAagtgagttcmAagttggattmAataaaaaatmAataaacaacmAataaatatamAataataaaaAatatctttataacattttg"AatatgaatgmAatatggatamAatatgtaggtAatatgtgacmAatattattamtAatattcatttAatattcgcctAatattttaatAatcacctgttAatcaggaggmAatcatcctatAatccagatamAatcctaatcmAatctacatgmAatctgatatmAatgaaaaatatgatgaagaAatgaacaagtAatgaagaaamAatgaaggaatAatgatagtatAatgatatgatAatgatatgtmAatgatattgiAatgatgcaatAatgatggtatAatgatgtcgtAatgatttaatgatatgtmmAatgatattgiatgatattgiiatgatattgiiatgatattgiiatgatattgiidwAcaatcttttttataacatcAcaattgaaatAcacacacattAcactgtagcmAcagaaagggtAcagaatgtttAcaggagattmAcaggatattmAcataacagaiAcataatattmAcatatatattAcatatgacctAcatcaccaattgciAccctaacgciAcgcaaaccciActtcgtgttttaaattttt!AgaaataactttttagtttcNAgaaatacttataagagata?AgaatattgagtttattaaaAgatagttagtaagctagct>AgatattattcaagcgatgtFAgatccaaatiAgattatgaaiAgcaaccaggiAgcaaggtaaaactccgctgAgcaaggtaaaactccgctg@{Aggatgattg@Agtgaagatg@ccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActccagaagmmActtcgtgttttaaattttt!AgaaataactttttagtttcNAgaaatacttataagagata?AgaatattgagtttattaaaAgatagttagtaagctagct>AgatattattcaagcgatgtFAgatccaaatiAgattatgaaiAgcaaccaggiAgcaaggtaaaactccgctgAgatatgatgmAgatatgtaamAgatattattcaagcgatgtFAgatccaaatiAgatgaatcamAgattatagamAgattatgaaiAgattggtctmAgcaaccaggiAgcaagaaaamgixAgtttttataattggattgatttataattggattgaAgtttttataattggattgaAgcaaggtaaaactccgctg    >TAnonymous markerU3D73D73D73D7V383 bpXYesY Provisional[ Middle Left130 kb230 kb145 kbEFH29696GATGCTTT ACAATATTCA TTAAAAACTT TTACAAGACA CCCCAATAAT AGAGTTGATG CACCY294 is a multi-transformant.m_AATAGTGAT ATATCTGATG AGAAGGCATT ACAAGATATA TACTTAAATT ATAAAGAGAA 4321 ATCAATTAAG TTATTGATTA TAGGTATAGG TATAACGAAT ACGGGAAAAT TATTTAATGC 4381 TGGAGGATGT AATATGAATG GGGATAATTG TCCACATGTA TATGCATCTA AAAGTTTTTC` 4441 ATATATTGGT GGTGTTGATA CATTTTTAGA AGTAAATAAA TGTGATAGTA GTATCGATAA 4510 4520 4530 4540 4550 4560 4501 TAATGGAGGT GACAATGGGA ATGGGACAGA AGGAAATGTA AATCCAGATA Y294 is a multi-transformant.m]A TGGATTGTTC CACAAATATA ATGAACAGTT TAGTTATAGA 4021 TAAAAATAAG GTACATGTTT CGTTGATAAT ATCTTTAGAA AAGAAGTCTG TTCATCAAGG 4081 CTTTGATGAT GTTAATTCTT ATAATAAAAA TGAATTAATA AAGACATTAG GAAAGTTAGA 4141 GAATTCGACT ^GTTTTAAACA AAACTAATAT ATTAGATTCG TTAGTATATG GTATTCAACA 4210 4220 4230 4240 4250 4260 4201 ATCTTTTGGA AAAGGAAATA GAGAAAACGC ACCAAAAGTT ACTATGTTAT TAACCAACAG 4261 TY294 is a multi-transformant.m[TGGT ATTGGAGGTC CTACTATTCA 3721 TAAATTAAGA TTGTTAGGTG GATGTGATAA ATCGGATGGT GACTGTCCAT ATGTTGTAAA 3781 AGCAGAATGG AATAATTTAA AATATACGTC AAATTTAATT ATTGATAAAA TATGTCATAC 3841 AGATAAGCCA GTAGAAAAGC CAGGAGAT\AA TTCATCTGTA TGTAATTCTA AGGATGATTG 3910 3920 3930 3940 3950 3960 3901 TATATGTGCG AATTATTTTG ACTTAACATA TATTGAAGTT CCAACATTAA ATAGTACATA 3961 TACATGGAGA AGTGATTTTY294 is a multi-transformant.mYTAAGAGA 3421 GTTCATTAAA TATGGACAAA AAGAAAGTTA TGATAAAAAT AACTTAGTAA GAAGAATACA 3481 CGATTTGAAA AAATATTATA AATCAGGAGG ATTTTCATAT ATAGTAGAAG CTTTGAAATA 3541 TGGATTATAT TCATATGCAA AAAGTACATC TTCTAGATTA AATGTZTCCTA AAGTAAATAT 3610 3620 3630 3640 3650 3660 3601 TTTGCTTACC GATGGGAATA ATACAGATAC GTCTGATTTT ATTTTAACTG AAGTGAGTTC 3661 CTTGTATAAG AAAGAAAATG TAAAGTTATT ATTGATY294 is a multi-transformant.mWTCAACA CCTGGAAGTC CTTCCGAGTC AACACCTGGA AACCCCTCCG AGTCAACACC 3181 TGGAAGTCCT TCCGAGTCAA CACCTGGAAG TCCTTCCGAG TCAACACCTT GCAGTGGAAC 3241 CGAATGTTTA TGCCACAATA CCTACGACTT AACATTAATT ATAGACGAAT CTGCAAGTAT X 3310 3320 3330 3340 3350 3360 3301 TGGATATTCT AACTGGGAAA AGGAAGTAGT TCCATTTACT ATAGGACTTG CAAGTAATTT 3361 AGAAATAAGT GAAAAGAAAG TGAATATGGG AATATTATTA TTTTCTGATA AAAY294 is a multi-transformant.mUAAAGGGATT ACAGGATATT GCATTATTAT ATAGAAAAGA 2881 AAATGTCAAA TTATTGGTAG TAGGAGTTTC TACAGCAAGT GAGAATAAAT TGAAAATGTT 2941 AGTTGGTTGT GCACCAAATG TTGTTTGCCC ATTTGTTATT AAAACGGAAT GGGGATTATT 3010 V 3020 3030 3040 3050 3060 3001 GAAAAGTGTA TCAGAAGTGT TTGTAAAAAA AATATGTGAC AATGGAGTGG TATTGCCACC 3061 AGGAAGCCCC TCCGAATCAA CACCTGGAAG TCCTTCCGAG TCAACACCTG GAAACCCCTC 3121 CGAAY294 is a multi-transformant.mST AAAAGAATAA ATATAAGTTA 2581 TAAAAATGTG CATATGGGTG TTTTGCTTTT CTCTGAATAC ACTAGAGATA TTGTTAGATT 2641 TTATGACAAT GCAAGATATG AAAAAGGGAC ACTACAGACA AAAATAAATG ATCTAAAAAG 2710 2720 2730T 2740 2750 2760 2701 AGATTATAGA AGTGGAAAGA AGACATATAT AATACAAGCT CTACGATATG CATTAACATA 2761 TTATAGCAAA CTTTCAAATA GAAAGGAAGC ACCTAAAGTA ACCATGTTAT TTACAGATGG 2821 AAATGATCCC TATGAATCAG AY294 is a multi-transformant.mQCTGA 2281 AGAGAATCCT GAACCAGTAG AAAAACCAAA TCCAGAGGAA AATCCTAATC CAGTGGAAAA 2341 ACCAACTCCA GAAGAAAATC CTAATCCAGT CGAAAAACCA ACTCCAGAAG AAAATCCTAA 2410 2420 2430 2440 2R450 2460 2401 TCCAGTGGAA AAACCAGAAC CAGAAAAGAA TCCTTGTATA AACATGGAAG ATTGCTATTG 2461 TAAAGATTTT TATGATATCA CTTTAGTTTT AGATGAATCA GCTAGTATAT CTGATTTGAT 2521 ATGGAGAAAC GAAGTTATTC CATTTTCTTT GGAAATTATY294 is a multi-transformant.Z`mCAATATA TATATATAAT 310 320 330 340 350 360 301 ACATAATATT TTTCCACCAA AATTTATAAA GTGGACATAA TTTTAAAGTC CTCCAGTTTT 361 TTGGAATTTT TTTTTTTTTT TTTATTATAA TATTCDATATG TCTTTATAAC AACGATATTT 421 TTATTTTTTA TGTATTTTTT TCCATATTTT TTTAAATATA TATTTGTATT TTGTTATATA 481 TTTATTATAT TCATATTTTT TTTTTTTTTT TTGTGTCGCC ATTTCTTTTA TATACATGTT 541 TAAGATCTAA GGCAATTCAT ATATTTTtGcgcacaaaagaaaatcctaqGctijGctacctctttatttccata?GctctatctttaataatcatAGgaagcaatataatgagtaaNGgtrTgaaggtaaaiTgaagtatatmTgaatataattTgaatatgggmTgaatatgtttTgaatgggaamTgaattaatamTgacacagcctTgagagactctTgagttgttctTgataaaaatmTgataaaactmTgataatgattTgatagaggttTgatcaacaatTgatcgttgatTgatgaaaacmTgatgacgattTgatgatatgtTgatgatgattTgattaggcamTgcaggttgtmTgcatctcaamTgcatttcccmTgccaaatgaiTgccaacaatiTgccacaatamTgcttagaatmTgcttctgccmTgcttttgtgmTggaaataatiTggaaatggtiTggaagcatgmTggaagtcctmTggaatgattrAW 3-0011BTGA TAC TAC CTC GGT GAG ACACTGT GTT CAG AAA TAA TGT TTC GAF190 bpGJT 120ATTATTTTTTATGATACTACCTCGGTGAGACATATGAATCATATTGGAATAATAAAGATTCGATATATTATTATTTATTTTAGATGCACCCATAAAACCCAGATGTGTATTATTCATATACATATTATTTATCTGATGAATAGAACCTCTTGTACTTCTATTATCTCTCAAGGATAATGTCGAAACATTATTTCTGAACACATTAATTGGTBbTTTATTCTTTCATTAAGTTCCTTTTTTATAATATTATATTTATTTGGATAATTCTTTCATTTTTAATAATAGATTATTATTTTCAACTCTTAAATTAT4IB8 210J3L27/11/96M17/1/97N114P3477/1/97N114P347Vr@Rs@t@BDFHJLNPRTVXZ\^`bdf h j l n prtvxz|\u@BLv@AVwB"D#!UfJ3K5Sequenced and primers ordered from both directions. L30/10/96M17/1/97N102P12215R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA450C annealing@A>    >TAnonymous markerU3D73D73D73D7XYesY Provisional[Left\125 kb85kb110^DBGF_6628A TCA ATT GTT CAG TTC CTTF150GJT33I42 554 1412J3L30/10/96M21/11/96N103P12615R3D7 MunI!h@WiBoDpFqHJhj@aUXk@Ynl@ZmCE`GIKMOQSUWY[]_acegikmoqs_n o@^q\ ^qVrgAW 3-0003BGAG TAT GAC AAT ATT GAA CACCTA TCA ATT GTT CAG TTC CTTF150GJT33I42 554 1412J3L30/10/96nI3D7 MunI3D7 MunI3D7 MunIA450C annealing@A>    >TAnonymous markerU3D73D73D73D7XYesY Provisional[Left\125 kb85kb110^DBGF_6628gAW 3-0003BGAG TAT GAC AAT ATT GAA CACCTA TCA ATT GTT CAG TTC CTTF150GJT33I42 554 1412J3L30/10/96IGarGaakGacfGactgtgtctttttcatctgGatfuGatccaatataccatcagcaGatcttaagatataaacattGattggtgtgatggttgatgGcctGctfikGgafGgagaagaaggagatacatt-sAW 3-0012BTGA CAA CAA TTA ATT CGT CTCTGA TTA TAT CGA ATT CTT ACC CAGY130LI130 B8 210 J3L27/11/96M17/1/97N115P23R3D7 MunITony Triglia size selectedTony Triglia size selectedonymous markerUB8B8B8V308 bpXYesY Provisional[Middle\175 kb^B_6sAW 3-0012BTGA CAA CAA TTA ATT CGT CTCTGA TTA TAT CGA ATT CTT ACC CAGY130LI130 B8 210 J3L27/11/96M17/1/97N115P23R3D7 MunITony Triglia size selectedTony Triglia size selectedsize selectedP 23d7stuvwB8 +,-.9:;OPQRSTUVWXYZ\]^_`abcgijkmnqrFtAW 3-0013BATG TTA TTA CAA TTT GAT GAT ATGCTAT TTC TCG CAC CTT GCC TAA TACD RNA POL 11F690 bpG RNA POL 11A 10 20 30 40 50 60 1 AAATAGAATA TAGATATATA AGAGAGTCAA TAAAATTAAT GTAATATATA TATATATATA 61 TAATATAATT ATATATAACA ATGACGGTTG ATTTGAATAT TCCATATTCA GCATG0 1 AAATAGAATA TAGATATATA AGAGAGTCAA TAAAATTAAT GTAATATATA TATATATATA 61 TAATATAATT ATATATAACA ATGACGGTTG ATTTGAATAT TCCATATTCA GCATG 15011510mt1520mt1530mt1540mt1550mt1560mt1561mt1621mt1681mt1741mt1801mt181imt1810mt1820mt1830mt1840mt1850mt1860mt1861mt1921mt1981mt20imt2041mt2101mt2110mt2120mt2130mt2140mt2150mt2160mt2161mt2221mt2281mt2341mt2401mt241imt2410mt2420mt2430mt2440mt2450mt2460mt2461mt2521mt2581mt2641mt2701mt 361t3610mt3620mt3630mt3640mt3650mt3660mt3661mt3721mt3781mt3841mt3901mt3910mt3920mt3930mt3940mt3950mt3960mt3961mt40imt4021mt4081mt4141 6481t650imt6541mt660imt6601mt661imt6610mt6620mt6630mt6640mt6650mt6660mt6661mt6721mt6781mt6841mt6901mt6910mt6920mt6930mt6940mt6950mt6960mt6961mt7021mt7081mt7141mt7201b88221t8281t8341t8401t841imt8410t8420t8430t8440t8450t8460t8461t8521t8581t901imt910imt920imt930imt940imt950imt960imt961imtAmAaaaaaaaaamtAaaaaaaaatiAaaaaaaaccmAaaaaaaagttAaaaaaaatttAaaaaaagaatAaaaaaattgtrsAaacataatttAaaccagaacmAaaccagttatAaacccaaaciAaacccaaatiAaaccccaatiAaacgaatattAaactaatatmAaactaatggtAaactcataatAaactgttgatAaagaaaatgmscAaaggtatgatAaagtacatcmAaagttacaatAaagttgcattAaagtttttcmAaataaaaaatAaataaaatttAaataacaaamAaataacgaaiAaataagagamAaatacatcgmAaatagaatatAaatagtagaiAaatatattcmAaatatgtcamAaatattacctAaatattatamAaatattcgctAaatatttgatAaatcaaagatAaatccaaatiAaatcctaatiAaatcggtagtAaatgatacamAaatgatcccmAaatgcaaaciAaatgggaaamAaatggttagtmAagagggatgmAagatatatgmAagatatcaamAagatgtgaatAagattcagtmAagatttagttAagcataggamAagcggtgtatAaggaccatamAaggaccatttAaggagatcatAaggatatagmAagggaaatgtAaggtgcatttAagtaaatatmAagtagaaagtAagtataactmAagtattaggtAagtcatatamAagtgaaacamAagtgaagtgtAagtgagttcmAagtggaaattAagtggagtatAagttcgtcaiAagttggattmAataaaaaatmAataaaaatatAataaacaacmAataaatatamAataaatgattAataacaatatAataagaaaatAataagaatctAataataaaadAattattcagtAattattttgmAattcaatatttatattattRAattcatttatAattctttaatattataaac[AattgataccmAattggtataiAattgttatamAatttaaatatAatttataaamAatttatcgttAattttcatttwFAccaaaacatiAccaaaagttmAccaaaatattAccaacatcatAccaactccamAccaattgtcmAccagaacctmAccataaaatmAccataatgttAccatctcctmAccatgtgacmAccatgtgcgtAccatgttatmAccattctcttAcccaaatgciAcccaaatgtiAcccatcttgmAcccattttctAccccaatgciAccctaacgciAcctaaaccttAcctaaagtamAcctaaatcatAcgaatctgcmAcgcaaaccciAcgcggatgamAcggaggaacmAcgggaaaatmActacagacamActagagatamActataataamActatgttatmActattgtagmActccagaagmActtaacatamyAcctgtttattAcgaatctgcmAcgaggacgatAcgcaaaccciAcgcggatgamAcggaggaacmAcgggaaaatmAcgtatgggttActaatgatatActacagacamActagagatamActagtggtatActataataamActatgttatmActattgatttActattgtagmActccagaagmActgttggtttActtaacatamActtattgaatx>Acctgtttat@Acttcgtgttttaaattttt@yAgaccttatg@y{AgaccttatgtAgacgaaatgtAgagaagatatAgagaaggtatAgagaatcctmAgagagtcaatAgagttgatatAgagttgatgmAgataagccamAgataataagmAgataatatctAgataatgcttAgatagttagtaagctagct>AgatatgatgmAgatatgtaamAgatattatttAgatattattcaagcgatgtFAgatattgattAgatccaaatiAgatctgagatAgatgaaagatAgatgaatcamAgattaaaagtAgattatagamAgattatgaaiAgattggtctmAgatttaggatAgcaaccaggiAgcaagaaaam{TAggatgattgmAggattattamAggatttgtatAggcaaacggtAggcacatgttAgggaaaggamAggtaaaaaatAggtgaatggtAggtgatgatmAggtgttccatAggtgttccttAggttataattAggttttgaamAgtaaataaamAgtaatagtatAgtaatattamAgtagaaaaamAgtagagaagtAgtagatatgmAgtatctaattAgtattaggttAgtcaacaccmAgtccaaggttAgtgaaccaamAgtgagtgtamAgtgataagciAgtgattttamAgtgctatggiAgtggaaagamAgtggtagtamAgttagaattmAgttagagaamAgttcttgtamAgttgaaacamAgttggttgtmAgtgaagatgtAgtgagtgtamAgtgataagciAgtgatgaattAgtgattttamAgtgctatggiAgtggaaagamAgtggacttttAgtggtagtamAgttaataattAgttagaattmAgttagagaamAgttagtgtttAgttcacctgtAgttctcatttAgttcttgtamAgttgaaacamAgttggttgtmAgttttgaagtAtaaatgtaamAtaaattatatAtaaattctctAtaaatttaatAtaacagtagmAtaacttcaatAtaagataatmAtaagcaaaamAtaataaaaamAtaataataaimtAtaataatagtAtaatattaatAtaatattggtAtaatatttatAtaatccatatAtaatgataatAtaatgatcttAtaatgctaamAtaatggtcgiAtacaaaacamAtacaaaatamAtacagatacmAtacatatacmAtacattattmAtacgagtaamAtacgcaatamAtagaaaagamAtagaatggtmAtagacgaatmAtagctgttgmAtaggacttgmAtaggttatgmAtagtacatamAtagtagaagmAtataaaagtm|AtaatgggaatAtaatggtcgiAtaattatattAtaattggcttAtacaaaacamAtacaaaatamAtacagatacmAtacatatacmAtacatcggctAtacattaaatAtacattattmAtacgagtaamAtacgcaatamAtactgatgatAtagaaaagamAtagaagcagtAtagaatggtmAtagacgaatmAtagaggagctAtagctgttgmAtaggacctttAtaggacttgmAtaggttatgmAtagtacatamAtagtagaagmAtagtccttttAtataaaagtmAtataaatactAtataacaatt|AtatattggtmAtatcatcagtAtatccaaagmAtatccattttAtatctatttmAtatctgatgmAtatgaaatttAtatgaatagtAtatgcataatAtatgcattatAtatgggtgamAtatggtttatattcatgat5AtatgtgacatAtattatttgaaattattgaPAtatttaaagiAtattttcctmAtatttttcamAtcaatggaamAtcaattaagmAtccaaatgciAtcctaatgciAtcctttcttmAtccttttttmAtcgacaaagmAtcggatggtmAtctaaaaagmAtctatcgaamAtctattttamAtctgacagtmAtcttcaacgmAtctttagaamAtcttttggamAtgaacagttmAtgaagtacam#AtattagtgamAtattatttgaaattattgaPAtattcccagtAtattcgatatAtattcgccctAtattgaacatAtattggaattAtatttaaagiAtattttaactAtattttatttAtattttcctmAtatttttcamAtattttttctAtcaaaaccatAtcaaaattttAtcaaataaatAtcaagaatttAtcaatggaamAtcaattaagmAtcaccaaaaAtccaaatgciAtcctaatgciAtcctttcttmAtccttttttmAtcgacaaagmAtcggatggtmAtctaaaaagmAtctatcgaamAtctattttamAtctgacagtmAtcttcaacgmAtctttagaamAtcttttggamAtgaacagttmAtgaagtacam}bAtcaccaaaatAtcacctttttAtcagcctattAtcagttgaatAtccaaatgciAtcctaatgciAtcctttcttmAtccttttttmAtcgacaaagmAtcggatggtmAtcgttaatttAtctaaaaagmAtctatcgaamAtctattgggtAtctattttamAtctccaaaatAtctgacagtmAtcttcaacgmAtctttagaamAtcttttggamAtgaaaattctAtgaacagttmAtgaagtacamAtgaatttcatAtgaccttgat}vAtgcaaaccciAtgcaaatcciAtgccgaatgmAtgcgtttcgmAtgctactattAtggaaaaaatAtggaacggamAtggaatttgmAtggagaaacmAtggagatattAtggagtagttAtggatcatcmAtgggacagamAtggtaagagatgatggctgAtggttttattAtgtaaaagatAtgtaaaatatAtgtaaagaatAtgtaataatcatggagaaaAtgtaggaaatAtgtataccatAtgtatcatttAtgtgaagaamAtgtgaagatmAtgtgccaattAtgtgtgtaatgattctaat9AtgttgtaaamAttaaaagtamAttaaattatiAttactcttgmAttagattcgmAttaggacatmAttatatttamePAtgtggtacatAtgtgtgtaatgattctaat9AtgttaataatAtgttacatttAtgttatcattAtgttatgtctAtgttgtaaamAttaaaagtamAttaaatatttAttaaattatiAttaacaccatAttactcttgmAttagataattAttagattcgmAttaggacatmAttaggtgggtAttataacaatAttataataatAttatatcattAttatatttamAttatcgacatAttatggaaatAttatgttgatAttattatcatcatatatgt@eAttgttctactAttttttaattatgctgtta@~Caaagagttt@Caatatgata@Cagcaataataagatgttaa@Cattttggtc@Ccaaatgcaa@lCccacatcacCgtgataaagaagaaaagtt@Ctgagagttt@Cttagtattatctaaatgac@Gaaaacggat@kGaagaaaatc@Gaattcctaagaaacaaata@Gaatttcttccactttttgt@TGataggtgtg@Gatatatttcacatgttaaa@Gatgatattatgttaagaga@jGccaccataaattattatct@iGgagaagaate~pAttgttctactAttgtttatatAtttaaatggtAtttaacttttAtttaagtggtAtttaatgaatAtttaattcttAtttacaaaatAtttagctacmAtttatcagttAtttatgttctAtttattatctAtttattatttAtttatttattAtttcttttamAtttgaatattAtttgatagttAtttgtattamAtttgttagatAtttgttattmAttttaactgmAttttcatatmAttttccacamAttttgatggtAtttttgaaamAtttttgtaam~8CaaagagtttmCaaataagaamCaaatattaatCaaatgataamCaaatgcttttCaaattaatctCaaattatagtCaacacaatctCaacaccagctCaagaacaaamCaagaagtattCaagattgttmCaagatttaatCaaggtgcgatCaagtaatttmCaagtacaacmCaagtagatttCaagtgctggtCaatacgttamCaatactacatCaatatacattCaatatcatamCaatgcaaatiCacaaatatamCacattaaaamCacattgccamCacccaaggtmCacctaaagtmCacctggaagmCaccttcatgmCaccttgtctmCacgtttttamCagaaaagaamCagaaagctcmCagaaccaaamTCaatatgatatCaatattaattCaatcaattgtCaatgcaaatiCaattttatttCacaaatatamCacaatttcctCacatcaccatCacattaaaamCacattgccamCaccaaaatatCacccaaggtmCacctaaagtmCacctggaagmCacctgtggctCaccttcatgmCaccttgtctmCacctttaaatCacgtttttamCacttcaccgtCagaaaagaamCagaaagctcmCagaaccaaamCagaagatactlJCattttggtctCatttttaattCatttttagamCcaaacgcaaiCcaaatctatmlCccacatcacCccacccatgttgattcctcYCccagatgagtCccatacagctCcccaacatctCcccaataatmCcctatgtggmCctaataaaaiCctaatgcaaiCctaatgccaiCctacaattamCctacgacttmCctatagatatCctatgttagtCctcatcatgtCctgatccaaiCctggaagtcmCcttcattactCctttaattatCcttttgatctCgaaaaaccamCgaagaaccaiCgaatacgacmCgaatcaacamCgaatgtttamCgatgaagcatCgatgatgagtCgatttaacamCgatttaactmCgatttgaaamCggaggaggamCgggtgaaattCggtgaatggmCggttattactCgtatgatgatCtgagagttttCtgatttgatmCtgcaagtatmCtgcatgttcmCtgctgctgamCtggaaaattmCtgtaatatctCtgtagtttctCtgtggataattcttggtccECtgttatatgmCttacggatamCttagcagcamCttagctgcttTXGataggtgtgtGatagtattatGatataaatatGatataaatgmGatatacatamGatatagtaatGgtttacaatGgttttactgtGgttttagatmGtaaaataggtGtaaagaaggtGtaaatagtttGtaaatgaaatGtaataataatGtaatatatatGtagaaaagcmGtagaaagccmGtagaatgtamGtagaattacmGtagatcctaiGtaggggttamGtagtcctagmGtagtgattgmGtagtggtgamGtatcatgggmGtatcgataamGtattcaacamGtcacttatgmGtcattattamGtccctgtccmGtcctcaatcmGtctagtaatmGtctgattttmGtcttaaaaaiGtgaaggttcmGtgatgaattmGtgattggtcmGtgcaacgtgmGtggacataamGtggagatttmGtgtaacttgiGtaatgaaacmGtaatttaaatGtacatgtttmGtaccaccgcmGtacgaagaatGtagaaaagcmGtagaaagccmGtagaatgtamGtagaattacmGtagatatattGtagatcctaiGtagattgtgtGtaggggttamGtagtcctagmGtagtgaagatGtagtgattgmGtagtggtgamGtataaatagtGtatcatgggmGtatcgataamGtattcaacamGtcacttatgmGtcattattamGtccctgtccmGtcctcaatcmGtctagtaatmGtctgattttmGtcttaaaaaiGtgaaggttcmGtgatgaattmGtgattggtcmGtgcaacgtgmGtggacataamGtggagatttmGtgtaacttgi$GtataatgcatGtatatatgatGtatattaggtGtatattcaatGtatcatgggmGtatcgataamGtatggagaatGtattaagaatGtattacaggtGtattcaacamGtattgaaaatGtcaaaaggatGtcacctgcatGtcacttatatGtcacttatgmGtcagccaggtGtcattattamGtccacattatGtccctgtccmGtcctcaatcmGtctagtaatmGtctgattttmGtcttaaaaaiGtcttatttttGtgaacaatatGtgaaggttcmGtgaatgcactGtgatgaattmGtgattggtcmGtgcaacgtgmGtgctagagatGtggacataamGtggagatttmGtggtatcattGtgtaacttgi$.GtgtaggagctGtgtaggtcatGtgtttgtgatTaaatgatattTaaatgtgaamTaaattaagamTaaattaatamTaacaaacaamTaacaaatatmTaacaagacatTaacagacagtTaacatttcttTaaccaacagmTaactcctaatTaagaaaatttTaagaacgcamTaagaagagctTaagaatacatTaagatctaamTaagatgtgttTaagattacgtTaagcataattTaagtgtaattTaataagcaatTaataataatmtTaataatagtmTaataatatgtTaataatatttTaataatgtttTaatacaacatTaatagataatTaatagtaattTaatagtgatmTaatagtgcamTaatataatttTaatattcaamTaatgataatmTaatgatgacmTaatgatgattTataacgaatmTataactaaatTataagaacatTataatattttTataatgctatTataatgctgtTataatgttatTatacatgttmTatactacattTatactttaamTatagaaaagmTatagaagattTataggaggatTatagtaaaatTatagttaagmTatataggtatTatatataatmTatatatatatTatatattactTatatcccaatTatatttatamTatcaaaagamTatcctgaagmTatcgatcatmTatgaatcagmTatgaatgatmTatgatatcamTatgatcggamTatgatgaagmTatgcatctamTatggacaaamTatggtatatmTatgtacaaamTatgtcatacmTattagatcaTatatggatamTatatgtgcgmTatattattamTatatttatamTatattttactTatcaaaagamTatcctgaagmTatcgatcatmTatcgctagctTatgaaagaatTatgaatcaatTatgaatcagmTatgaatctttTatgaatgatmTatgatatcamTatgatcggamTatgatgaagmTatgatgatgtTatgcaaaattTatgcacttttTatgcatctamTatggacaaamTatggacaggtTatggatatatTatggcatgctTatgggaggttTatgggtgtttTatggtaaaatTatggtatatmTatgtacaaamTatgtcatacmTatgttttcgtTattaaacaatTattaaagaatTattagatca+TattaaattttTattacaatttTattagatcaTattaaattt@TattagatcamTcaggtatttggccctccag@Tccgtacattttctacatct@Tccttccgag@Tcttctccat@Tgaaggtaaa@Tgatgatttt@TggaatgattmTgatgatttttTgattaggcamTgcaggttgtmTgcatctcaamTgcatgtaaatTgcatttcccmTgccaaatgaiTgccaacaatiTgccacaatamTgccgatagctTgctgaagaatTgctggtgtatTgcttagaatmTgcttctgccmTgcttttgtgmTggaaataatiTggaaatggtiTggaagcatgmTggaagtcctmTggaatgattTtacagaaaatTtacagatggmTtaccaattatTtagaaatgaiTtagatgatatTtagtatatgmTtagtatggttTtataaacacmTtatagcaaamTtatatattctTtattgattamTtattggtagmTtatttagaatTtatttttatmTtattttttamTtattttttatgatactaccrTtattttttttTtcaaaaaagmTtcaacatcatTtcaatatggtTtcaattttttTtcaccaacatTtcacctacatTtcaccttcatTtcatcaaggmTtcatcaccctTtcatctgtamTtccatattttTtcctttaataaaaaaaacaTtctaaaaaamTtctaaaaagmTtctaaatgaiTtctagattamTtctccaaattTtctccgaaatTtctctaatatTtctgaatggmTtctgcatctmTtgaaaaagamTtgaaaaggctTtgagaaaagmTtgcacattgmTtggaattttmTtggagaatgmTtgaaactagtTtgagaaaagmTtgagaggaatTtgataaatttTtgatgaacatTtgattatattTtgatttgtatTtgcacattgmTtggaattttmTtggagaatgmTtgggagagat~TttatgtgcgtggtattgtaDTttattaaaatTttattataamTttattatatmTttattatcatTttcatataatTttccaccaamTttcctttatttcgtacgBTttctctaccmTttgacatgtmTttgataagccatattacta:TttgccgaaamTttgcttaccmTttgtgaatamTttgttttttmTtttaaagtcmTtttaactttmTtttaatatamTtttaatggatctataattaCTtttacaatamTtttataatagcagctcttg-TtttatttattattttatatTtttatttttmTtttctgatamTtttgcttttmTtttggaaatmTttttcttttmTtttttctttmTtttttgcatmTttttttcttmTtttttttttmTttcgattttttatcgtacgBTttcgtttaatTttctctaccmTttcttgttgtaatatagcawTttgacatgtmTttgataagccatattacta:TttgatataatTttgatgaagtTttgccgaaamTttgctaagctTttgcttaccmTttggattcctTttgtaagtatTttgtgaatamTttgttaaaatTttgtttggatTttgttttttmTtttaaacattamTtttaatggatctataattaCTtttacaatamTtttataatagcagctcttg-TtttatttattattttatatTtttatttttmTtttctgatamTtttgcttttmTtttggaaatmTttttcttttmTtttttctttmTtttttgcatmTttttttcttmTtttttttttmKTtttaaagtcmTtttaactttmTtttaatatamTtttaatggatctataattaCTtttacaatamTtttataatagcagctcttg-TtttatttattattttatatTtttatttttmTtttcaccaatTtttccaagatTtttccatgctTtttcgatgttTtttctgatamTtttgcttttmTtttggaaatmTtttggatcctTtttgttacctTttttacatatTttttagaaatTttttataactTttttcttttmTttttgaccatTtttttctgttTtttttctttmTtttttgcatmtTttttttcttmTtttttttattTttttttttgtTtttttttttmtBTGAAT 121 TAAAGAGAGT AAAACGATTA GAGTTAGGTG TTTTGGATCC AGAAATAATA AAAAAGATAA 181 GTGTTTGTGA AATAGTAAAT GTAGATATAT ATAAAGATGG TTTTCCAAGA GAAGGTGGAT 241 TAAATGATAT ACGTATGGGT ACTATTGATT ATAGGACCTT ATGTGGTCACA TGTAATATGA 310 320 330 340 350 360 301 ATGTAAAATA TTGTCCTGGT CATTTTGGTC ATATAGAATT AGCGAAACCT ATGTATCATT 361 ATGGTTTTAT GAATGTAGTA TTAAATGTTT TAAGATGT >TKnown gene sequenceU3D7[ Right MiddletDGT ATGTTATCAT TGTGGTAGAT 421 TATTATGTAA TGTGAACAGT TCTAAAGTTA AATATATTGA AAAGATTAAA GTAAATAGTT 481 TAAGATTACG AAAATTAGCT GAACTGTGTT TAGGAATAAG AGCATGTGAT CATTCTGTAG 541 AAGAAGAAGG ATTAAATATT AACGATAATTE CTTTAAATAA TTTTTATAAC AATGATTTAA 610 620 630 640 650 660 601 GTAATTTAAA TATGAATCAA CAAATGCTTT TAAATAAAAG TAATTATACG AACATATTTG 661 AAATGGTTAG TAAAGAAGAT tFGTAGATTGTG GATGTGTTCA ACCAAAATAT AGTAGAGAAG 721 GACCAAATAT GTATATTCAA TTTTTACATA GTAGTGAAGA AGATATTGAT GAGAGTAAAA 781 GAAAATTAAG TGCTGAAGAA GCATTAGAAA TATTAAAGAA AATTAGAAAA GAAGAGATGA 841 GTATATTAGG ATGTTAATTCT GATAGGTGTG TACCAGCTTC TTTAATATTA ACATGTATAC 910 920 930 940 950 960 901 CTATACCTCC ACCATGTGCG AGACCTTATG TTCAATATGG AAATCAAAGA AGTGAAGATG 961 ATTtHTAACTTT AAAATTATTA GATATAGTAA AAACGAATAT ACAATTGAAA AGGCAAACGG 1021 ATAGAGGAGC AAAATCACAT GTATTACAGG ATTTATGTTC CTTATTACAA TTTCATATAA 1081 CTACCCTTTT TGATAATGAT ATTCCAGGTA TGCCGATAGC AACCACACGA TCTAAGAAGC I 1141 CTATAAAAGC TATAAGAACA AGATTAAAAG GTAAAGAAGG AAGACTAAGA GGTAATTTGA 1210 1220 1230 1240 1250 1260 1201 TGGGTAAAAG AGTGGACTTT TCAGCAAGAA CGGTTATTAC AGGAGATCCA AAtJTTTAAATA 1261 TTGATTATAT AGGTGTTCCT AAATCGGTAG CTATGACATT AACATTTTGT GAGACAGTAA 1321 CACCTTTAAA TTATGATAAT TTAAAGAAGC TTGTAGAAAG GGGTCCATAT GAATGGCCTG 1381 GAGCAAAATA TATTATTAGA GATAATGGTA CAAAATATGA TTTAKAGACAT GTACGAAGAA 1441 ATTCAGAGAA AGAATTAGAG TATGGATATA AAGTAGAAAG ACATATGACC GATGAAGATT 1510 1520 1530 1540 1550 1560 1501 ATATTTTATT TAACAGACAG CCTTCATTAC ATAAAtLATGAG TATTATGGGT CATAAGGCCA 1561 AAATATTACC TTATTCAACA TTTCGTTTAA ATTTATCAGT CACTTCACCG TATAATGCTG 1621 ATTTTGATGG AGACGAAATG AACTTACATT TAGCTCAGTC ACATGAAACA AGATCTGAGA 1681 TTAAACATTT AATGATAGTA CAAAGACMAAA TAGTTTCACC ACAAGGTAAT AAACCAGTTA 1741 TGGGTATAGT ACAAGATTCC TTATTAGCTA TAAGAAAATT TACAAGAAGA GATAATTTCC 1810 1820 1830 1840 1850 1860 1801 TTACAAAAGA AGAAGTTAtNTG TCCTTATTAA TTTGGATTCC ATATTGGAAT CATGTGATAC 1861 CAACACCAGC AATAATAAAA CCAAGGGCAT TATGGACAGG TAAACAAATT TTTTCGATGT 1921 TATTACAATT TGATGATATG AATATAGAAG ATGATAAAAA TGACACAGCC AATAATAAGG 1981 TTGGGAGAGAO TGTTAATACA AATGTTAACA AGGATAGTAG CAAAATGAAT ACTAGTGGTA 2041 ATTATTATTA TGGTAATTCA ACTAATGATA ATACTGATGA TTATTTAGAA AAGGGAAATG 2110 2120 2130 2140 2150 2160 2101 tPCATATTCAAG AAGTGGAAAT AATCATCCTA ATAGTCCTTT ATCTATTGGG GATAATATAA 2161 ATGTAGGAAA TGTACAGCAA AATGATATGA GCTCCCCCAA TAATAATAAT AATAATAATA 2221 ATAATAATAA TAGTAATAAT AATAATAATA ATAATATTGG TGGAGGTATT AATTCATTTQA 2281 AACGTTTTAA TATGGTAAAA ATAAATTTAA TGAGAGACTC TTCAACATCA TCTAAAGATG 2341 ATAATCCATA TTGTTCAATT AATGATGGTA AGGTTATAAT AAAAAATAAC GAATTATTAA 2410 2420 2430 2440 2450tR 2460 2401 GTGGTATCAT ATGTAAAAGA ACTGTTGGTT CTTCTAGTGG ATCGTTAATT CATGTTTTAT 2461 GGCATGAAAT GGGTCCAGAT AAAACGAAAG ATTTTTTATC AGCTTTACAA AAAGTTACAA 2521 ATAATTGGCT TGAATATGTT GGTTTTACTG TGAGTTGTTC ASGATATTATT GCAAGTAATA 2581 AAGTATTAGG CAAGGTGCGA GAAATATTAG ATAAATCTAA AAGTGAAGTG TCAAAACTTG 2641 TTGAAAAGGC ACAGAAAGGG GAATTAGAAT GTCAGCCAGG AAAATCATTA TATGAATCTT 2710 2720 2730 tT 2740 2750 2760 2701 TTGAAACTAG AGTTAATAAT GAATTAAATT GTGCTAGAGA AATGGCTGGA AAAGTTGCAT 2761 CTGAGAGTTT AGATGAAAGA AATAATATTT TTAGTATGGT GGCTAGTGGG TCAAAAGGTT 2821 CTATTATTAA TATATCCCAA ATTAUTATCAT GTGTAGGTCA ACAGAATGTT GAAGGAAAAA 2881 GAATACCATT TGGTTTTAAT CATAGATCTT TACCTCATTT TATTAAATTT GATTATGGTC 2941 CTGAGAGTAG AGGATTTGTA TCAAATTCTT ATTTAAGTGG ATTAACACCA CAAGAAGTAT 3010 tV 3020 3030 3040 3050 3060 3001 TTTTCCATGC TATGGGAGGT AGAGAAGGTA TTATTGATAC TGCATGTAAA ACATCTGAAA 3061 CAGGGTATAT ACAAAGAAGA TTAATAAAAG CCATGGAAGA TGTTATGGTA CAATATGATA 3121 GAACTGTWAAG AAATTCATAT GGAGATATTA TTCAATTTTT GTATGGAGAA GATGGTATGG 3181 CAGGTGAATA TATAGAAGAT CAAATTATAG ATTTAATGAA ATTAGATAAT AAAGAGATTA 3241 ATAAATTATA TAAATATAAT TTTGATGAAG AACCATTTGG AAAGGATTAT TATATAGGTA tX 3310 3320 3330 3340 3350 3360 3301 ATAAAAATGA TGGTAGTAGA AATACTACGT ATATAGATTA TAATAAGCAA AATATTTTAA 3361 ATCAAGAATT TGAAGAATTA TATAAATGTA AAAATTATTT ATGTAAAGAA ATATTCYCCAG 3421 ATGGAGATAT AAGACAACAT TTACCAATTA ATATGAATAG ACTTATTGAA TATGCAAAAT 3481 CACAATTTCC ATGTATACCA TTTGTAAGTA ATAATAATAG TACAAACAAT AATAATAATA 3541 ATAATAATAA TAATAATATT AGTAATAGTA GAAAACTTAT GGATAAGGtZGT AATTTATCGT 3610 3620 3630 3640 3650 3660 3601 CTACACATAA TCATAAGGAA AATAAGAAAA GAAGAAAAAG AAGAAGGAGA AAAAATAAAT 3661 TTGATAAATT TAAAAATGAA AATAATGAAC TTATGTCTG[A AATTAAAAAG GAGTATGAAA 3721 ATAATGATCT TAATAATATG ATGATAAGTA AAGGAGATCA ATCACCTTTT AAAGGTATGA 3781 ATGAATTTCA TATGGGTGTT GCAGATAATG ACATGGGATC AGATTTAGGA AATAATAATA 3841 ATTATAACAA TGATGATTTT GTTGATGATG t\ATTATGTTGA TGATGATGAT TATGATGATG 3910 3920 3930 3940 3950 3960 3901 ATGATTATGA TGATGACGAT TATGATGATG ATGACCTTGA CGATGATGAG AATTATTCAG 3961 ATAATATTAA TATAGGAGGA A]ATAGAAAAT ATTATGGAAA TACCTTAAAA AATAATTATG 4021 ATGAAAATTC CATGTTAAAT CCAATTGATG TTGTACATAA AGTTAATAAT TTTTTAGAAA 4081 AATTAGTAAT TATTAAACAA ATAAATAGTA ATGATACTTT ATCAGTTGAA GCACAAAATA 4141 ATGCTACTAT TTTt^GTTAAAA GCACATTTAA GAACTTATTT GAATTCAAAA CTTTTAACTC 4210 4220 4230 4240 4250 4260 4201 AAACTCATAA AGTTAGTGTT AAAGGATTAG ATTGGTTATT ACAAGAAATA GAAAAAATAT 4261 TTTA_TAAATC CTTATGTCAT CCAGGAGAAT GTGTAGGAGC CTTAGCTGCT CAATCAATTG 4321 GGGAGCCTGC AACTCAGATG ACATTGAATA CATTTCACTT TGCTGGTGTA GGTTCAAAAA 4381 ATGTTACATT AGGTGTTCCA AGATTAAAAG AATTAATAAA TATAGTAAAA AATGTAAAGA t`4441 CTCCATCAAC AACAATATAT TTAGATGATA TGGTTTCAAA TGATCAACAA AAAGCTAAAG 4510 4520 4530 4540 4550 4560 4501 ATATTTTAAC AAAATTAGAA TATACTACAT TGAAACAATT AACTTCACAT GCAaCAAATTA 4561 TTTATGATCC TAATACAACA ACAACTATTT TAGAGGAAGA TAAATCATGG GTTAATGAAT 4621 TTTATGAATT CCCAGATGAG GATGATACTC AATATTCATT AGGTGAATGG GTATTAAGAA 4681 TACAATTAAC CAATATACAT GTAAATGAAA AAAAATTAAC TATGAtbAAGAA ATTGTTTATA 4741 TTATATATTC TGTCTTTTCA AGTGATGAAT TAGATATTAT ATATACAGAT GATAACTCAG 4810 4820 4830 4840 4850 4860 4801 AAGATTTAGT TTTAAGAATT CGGGTGAAAT ATTTAAcATGG TGAATATAAT TTTATGAATT 4861 ATGATGTTGT AGATAATGCT AATGAACAAG TTGATGAACA AGAAGAAGAT GAAGAACACT 4921 TAGTTGCTAA TGATAGAGGT AATTACGATG AAACAAAAAA TAGTACTCAT CCTCATCATG 4981 ATTATAATAA CAATACTACA AATATATT tdTA AGTCCAAGGT AAAAAATAAT ATATCATCAG 5041 ATATAAATAC AAAGAATGAA GATAGTATTA GTATAAATAG TAGTAACAAT GAACAAGTAA 5110 5120 5130 5140 5150 5160 5101 AAAATATTAA TTCATCACCeC GTTTCAAATA ATATGCATAA TAATAATAAT AATAATAATA 5161 ATGATAGTAG CAATATTAAT GATATTAAAG TGAAGAATAT AAAAAAAGAA GATGGAAATG 5221 AAGGTGCATT AAGAGGCGGT GGTGATTCTA ATACATCGGC TTTGTTTGGA AATAAAAATA 5281 GTCAAAAGGA  tfAGATAATATC GTGAACAATA ATAATGATAA TAATGATGAT GATGATGAGG 5341 AGGAGGAGGA GGAAGACTTT TTGTTTGGTG ACCATAATGT ATCTCCAAAA AATACGAAAG 5410 5420 5430 5440 5450 5460 5401 AgTGGAAAAAA TAAGAATACA AACAACAAAA GTAATAATAA TGAAAACAAA AACAAAAAAA 5461 GCGGAAATAA TAATAGTAAT AATAGTAATA CGTATGATGA TGGTGATGTT GATAATGATA 5521 ATGATGACGA TAATGATGAT AACAAGAGTG ATATAACAAT CAAAGAGGAC AATGATGTCG  th 5581 CATTCATGAA AACAAGCACA AAAAATGCAG AAGAAGATTT AGAACTTAAG AATAAGAATC 5641 ATATTGAACA TAACATTTCT AGAGAAGATA CAGAAGATAC GTTTTTAAAA AAACTAATGG 5710 5720 5730 5740 5750 i 5760 5701 AACAATGTTT GTCCACATTA AAATTAAGAG GTATTGAAAA TATAACTAAA GTATATATGA 5761 GAGAGGAATC CAAAATAACA TACGATTCAG ATAATGGGAA ATTTGTTAGA AGTTCTCATT 5821 GGGTATTAGA TACTGATGGA TGTAATTTAG AAAATATATT TT  tjGTGCACCA CAAGTAGATT 5881 TTAAAAAAAC AGTATCTAAT GATATTGTAG AAATATTTGA AGTATTAGGT ATAGAAGCAG 5941 TAAGAAGAGC TTTATTAAAA GAATTAAGAA CTGTAATATC ATTTGATAGT TCATATGTTA 6010 6020 6030 k 6040 6050 6060 6001 ATTATCGACA TTTATCAATA TTATGTGATG TTATGACACA AAAGGGTTAT TTAATGTCTA 6061 TAACAAGACA TGGTATAAAT AGAGTTGATA AAGGACCATT AATTAAATGT AGTTTTGAAG 6121 AAACTGTTGA AATATTATTA GAAGC  tlAGCTG CATTTGCTCA GGTAGATAAT TTGAGAGGAA 6181 TTACAGAAAA TATAATGTTA GGTCAATTGT GTAAAATAGG AACTGGTTCA TTTGATATAA 6241 TAATAGATAA TCAAAAATTG AATGATGCAA ATCAAAATTT AGAAACTATT CAAGATTTAA 6310 m6320 6330 6340 6350 6360 6301 CAAGTGCTGG GTTTACAACA CCAGATAGTT TACATGTTAT AACACCTGAT GGTTTACAAT 6361 CACCTGTGGC AATTAATACG ATAAATTCTC CTTTACCATT TTCACCAACA TATAATGCTA 6421 ATTTATTA tnTC TCCTACAGCA CCTATAGATA ATGTTAATAA TTTATTATCA CCACAATATA 6481 ATTTACAAAA TTATGGAGAT AATGTAATGT CCCCAACATC AAAAGATATA AATAATTTAG 6541 ATACATTAAA ATTAGGTGGG AAATTTTCAC CAACACAATC ACCTAAATCA CCAACATCTG o 6610 6620 6630 6640 6650 6660 6601 TTATGCATTC ACCATTCTCT CCTTTTGATC ATCAAAACCA ACAACCAGTA GATGCAACCA 6661 ATTTATTATT TTCTCCGAAA AATAATAATA TTATGAATTA TAATGTATTC TCACCTA tpAAC 6721 CAAATATTAA TAATAATGTT ATTCAATCAC CTAATATATA TTCTCCAAAT CCTATGTTAG 6781 ATATTTTTTC ACCTAAACCT CAAATTAATC ATAATATTTA TTCACCTTCA TATTCACCAA 6841 CATCACCTAC GTATAATGCA AATAATGCTT ATTATTCACC AACCTCACCqA AAAAATCAAA 6910 6920 6930 6940 6950 6960 6901 ATGATCAAAT GAATGTAAAT TCGCAGTATA ATGTTATGTC ACCTGTTTAT TCAGTAACAT 6961 CACCAAAATA TTCACCTACA TCACCAAAAT ATTCACCTACtr ATCACCAAAA TATTCGCCCA 7021 CATCACCAAA ATATTCGCCC ACATCACCAA AATATTCGCC CACATCACCA AAATATTCGC 7081 CCACATCACC AAAATATTCG CCCACATCAC CAAAATATTC ACCAACATCA CCAGTTGCAC 7141 AAAATATTGC TAGTCCAAAT TATTCACCTT AsTTCAATAAC ATCACCAAAA TTTTCACCAA 7210 7220 7230 7240 7250 7260 7201 CATCTCCAGC ATATTCGATA AGTTCACCTG TGTACGACAA AAGCGGTGTA GTGAATGCAC 7261 ATCAGCCTAT GTCACCTGCA TAttTATTTTAC AATCACCTGT GCAGATAAAA CAAAATGTAC 7321 AAGATGTGAA TATGTTTTCG CCCATACAGC AGGCACATGT CGATGAAGCA AAAAATGACG 7381 ACCCATTTTC TCCAATGCCT TACAACATAG ACGAGGACGA AATGAAGGAA AATATGTAGG 7441 ATGGAGTAGT TAAGuCATAAT AAATAAAATT ATGTGCCAAT ACACACACAT ATATATATAT 7510 7520 7530 7540 7550 7560 7501 ATATATATAT ATATATATAT ATATATATAT ATATATATTT ATATATATTT ATTTATTTAT 7561 TTATGtvTATAT TTTTTTTTAT TATTCCCTCA TTTTGTTACC ATTATTTCAA TTCCATATTT 7621 TACTTTTTAT CAATTTTATT TTATTTTTTT TTTTTTTTTG TGTTGAAATG TGTAATATAC 7681 ATATGAAATT TTAAACCTTT TGGTGTCGCA ATTGAAGTTA TATATATATA TATATATATA 7w741 TATATATATA TATAATATTT TTTTTTGCAT AAAAAAAAAA AAAAAAAAAA AATAATATAT 7810 7820 7830 7840 7850 7860 7801 ACATATATAT GTCTTATTTT GCATTTGAAC AAAAAAAATT AAAAAAATTG TAAGtxTGTAAT 7861 AAAAAAAAGT CCTTTAATTA AAAAATAAAA AAATAAAAAA ATAAAAAATA CATAAATATA 7921 TAAATACATA AATATATGAA GATATAAATA TATATATATA TATATATATA TTATTCCATT 7981 TCAATATATG GATAAACAAA ACATTTAACA TATAAATGTG TTTGCTyAAGC ACATTTGTAT 8041 GGTTTAAAAA AAAAAAAAAA TAGATAGATA GATATTTGCT TTATTCTAAC AATTTTCATT 8110 8120 8130 8140 8150 8160 8101 TTTTAAACAT ATAATTATAT AAACATAATT ATCAAATtzAAA TCAAATAAAT GATATTGTGG 8161 TAAAATAATA TTTAAGAAAT CATTTCTTAT GTCACTTATA ACATTTTTGA TATCGCTAGC 8221 TGATCGTTGA TATATATTAC CTTTATGATA AACAATTTCT TTTCCTTTAT TAGATATGAC 8281 ATATGCATTA ATATGTGACA TGGGTGATA{T TTTATGTAAC ATATCCATTT TTAATCTTTT 8341 TTTAATAGTA CTACTTTCAT CTGTAGTTTC AATAACAATA TATGCACTTT CATTTTTAAT 8410 8420 8430 8440 8450 8460 8401 TTTATATTCT TTTAATGTTAt| TTAAAGTTTC TAAAAAGAAA GATCTACTAG AATAAATGAT 8461 TTGATTTGTA TTAACATCTC TTAATACTTC TATTTTAATA ATTGTTCTAC AGGTAAAAAA 8521 AAGTGGAGTA TATGGCATGC TAGGTAAATT TTTTTGACCA TAAAAAATAT ATAACTTCAA 8581 AAATTCATTT T}0TCTCTAATA TAACTCCTAA TTTTTTCTGT ACAAGGAATT C NAQ1hI130J3KSequence from malaria databaseL27/11/96M17/1/97N116P2R3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D7[ Right Middle/uAW 3-0014BCCA TTA AAG CCG ACT AAG GTCCAG GAT AAT TAT CAC ACA CTT GF1.3 kbGpF 3.9AAAAAGATTTTTATTGGTACCATGGTGTGAACCTGTAAATTATAAAAAGTCAGATATAATATTTAATACATATTTAGATATACTAAATAATGATTCTATTTTCATGTTAGAGAAAATAATTTTTGTATCTAAAAATGGAGATAAAATAGTTAAAGAATTGAATGTAGAATATCAAACATTTTTTGTTCATCCATTTAATTTATTATTATTATTTAATTTATTATTATTA\IP` )*56789:;<=\]^_jmqp !"#$%'()*+,-.0KLMTUVafgjmnrs !"#$'(/01345./0123456IJKLSTUVfgivw"#$'-./0STYZaijnu‚! !"#()*+./0?@HIkstuuBTTATCAATAGATAATGAGCACCCGAACAATCAAAGAATTAGAACATTTGAGACATATTATAATCAAACGCTTACATGTAATCATTATAATAATGGTTTACAAGTGTGTGATAATTATCCTGAATTC........GAATTCCATTAAAGCCGACTAAGGTATACAAATTGAATGTTAGTATTGATGAATATAATCAGAATTTGCTTTATGCCTCATAAGAATTTGAAATATCCTAATAAATATTATTCTAATGTTTACATATTGATGTTCAAGGTAGCCTATTTGTTTAATAATTATGACTTTTATACATTCTTTTATGATTTAACTGGAATACGTTTAATAGATTCTAATGTTATCAGTAATATGGGTGAATTFI592 993 1066 1079J3KFPrimers ordered from two sequencing reactions - one in each direction.SvAW3-0015BACA AAC ATA TTG ATC ATC ACTGA AAT AAC CTA TGT AGA F160 bpGJT108D7 MunI3D7 MunI3D7 MunIS45oC annealingTAnonymous markerU3D73D73D73D73D7[Right\40 kb160 kb175 kb^BCAB_491011vAW3-0015BACA AAC ATA TTG ATC ATC ACTGA AAT AAC CTA TGT AGA F160 bpGJT108  0@P`p  ‚! ‚" ‚$ ‚%‚&‚(‚)‚*‚1‚2‚3‚4‚5‚6‚7‚8‚9‚:‚A‚B‚C ‚D!‚E"‚F#‚G$‚H%‚I&‚J'‚Q(‚R)‚S*‚T+vAACAAAAATGAAATAACCTATGTAGATATAAAGAATAATTTTACATTTAATTTTAAAAAATTAAATAATAAATATAATCCATTTGGTATATTGAATGAGATGAAAAATAAGAATAGGGCACCTAATATTTACTCTGGAAATTATAATGATGATCAATATGTTTGTAATAATAATAATAATAAATACAATAATAATAATGTTGATTGTATAAB?ATAATAAGATTACTTTTTCAGGTGAACATTGTGATTATTTATTTCGACCTTTTAGAAAACTCAI872 1079J3L15/1/97M17/1/97N118P1012R3D7 MunI3D7 MunIA445oC annealing@A>    >TAnonymous markerU3D73D7"XvV273 kb[Right\165 kb175 kb^AB_811wAW 3-0016BATAAAGACATGAAATCATGTGCGTACATGTACAGATCTTAGAGF~ 200 G3/A12AGTCGATAAATTAACATTTTCTCTCGATCATAGAGTTAGAGATAATTTAAAAACAGATTTGATTTTAAATAATAATGGGGAAAATGATTATGCTTATTTAAACAAATACGTTTATACTATATTAAATCGTGATTCAACAGAAAAAATTAAAACATTTTTTTCTCATAATAAAGACATGAAATCATGTGATTATTTTATATCAAAAGAATATD7 MunI3D7 MunIA445oC annealing@A>    >TAnonymous markerU3D73D7uL27/11/96M17/1/97N117P711121214R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIS45oC annealingTAnonymous markerU3D73D73D73D73D7[Right\40 kb160 kb175 kb^BCAB_491011t\110 kb^C_10@ABCDEFGHIJKLMNOPQRSTUVWXYZ\]^_`abcgijkmnqrsuusTony Triglia size selectedS45oC annealingTYAC endU3D7B8B8B8XYesY Provisional[Middle\110 kb175 kb^CB_106rRTony Triglia size selectedTony Triglia size selectedTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerUB8B8B8V308 bpXYesY Provisional[Middle\175 kb^B_6qAtaacagaaccgtcaacaac%AtatcgtagaatgtttgcttAtatcttcagatactggaac[AtattacaaggtttaggaggAtattatgcttcaaagattc]AtattcatacaatttctgagAtcvAtcagtaaaaattattgaccAtgijtAtggctttctatactgtttcSAttfgsAttatatttaaagttttcagTAttattaatataatctttca AttgaaaaagcaaagagaaaAttttctaaattaccattct"AtttttttcaaatatagaaaCfkCagCaafst