Technical overview of the cell sorters
Sorter |
FACStar |
MoFlo |
DiVa |
Aria-C |
Aria - W |
| Lasers | 1: 488nm* 2: 561nm |
1: 488nm 2: 640nm and 561nm 3: UV 351-364nm*** |
1: 488nm* 2: 595nm** 3: UV 351-364nm |
1: 488nm 2: 405nm 3: 375nm 4: 640nm |
1: 488nm 2: 375nm AND 405nm 3: 561nm 4: 640nm |
| Nozzle Size | 70 and 100 microns | 70 microns | 70 microns | 70 and 100 microns | 70 and 100 microns |
| Sort Tubes | Clear Falcon tubes #2003 12x75mm Polystyrene |
Opaque Falcon tubes #2005 12x75mm Polypropylene |
Clear Falcon tubes #2003 12x75mm Polystyrene |
Clear Falcon tubes #2003 12x75mm Unrestricted |
Clear Falcon tubes #2003 12x75mm Unrestricted |
|
Collection |
12x75mm plastic or glass 1.5ml eppendorf Plates: Terasaki or 6-96 well |
12x75mm plastic or glass 1.5ml eppendorf Plates: Terasaki or 6-96 well |
12x75mm plastic or glass 1.5ml eppendorf Plates: Terasaki or 6-96 well |
12x75mm plastic or glass 15ml yellow cap 1.5ml eppendorf Plates: Terasaki or 6-96 well |
12x75mm plastic or glass 15ml yellow cap 1.5ml eppendorf Plates: Terasaki or 6-96 well |
|
Optimum Cell |
5x106 cells/ml | 4x107 cells/ml | 3x107 cells/ml | 3x107 cells/ml | 3x107 cells/ml |
|
Typical Sort |
8,000 cells/sec | 10-30,000 cells/sec | 10-20,000 cells/sec | 10-20,000 cells/sec | 10-20,000 cells/sec |
| Number of Sort Populations |
1 or 2 or Plates | 1 to 4 or Plates | 1 to 4 or Plates | 1 to 4 or Plates | 1 to 4 or Plates |
| Sort Region Type | Rectangular | Polygonal | Polygonal | Polygonal | Polygonal |
| Running Pressure |
7.5 - 28psi | 55psi | 30psi or 55psi | 20psi, 35psi or 70psi | 20psi, 35psi or 70psi |
* May be switched to 458, 476, 515nm.
** May be adjusted continuously from 550-650nm.
*** May be switched to 405nm.
Allow 30 mins set-up time for any of these changes
For more details about possible choices of fluorochromes, see Fluorochrome Combinations.
Collection Tubes
1-2mls of neat FCS or media containing FCS (whatever your cells will be happiest in). The tubes can be either glass or plastic (eg Falcon 2003 or 2005)
Removal of dead cells and debris
For smooth running of the sort and maximum yield of cells, run your cells through a dead-cell-removal column (glass Pasteur pipette with a plug of cotton wool in the end) or use a 100um filter prior to sorting.