Information for new users of the cell sorters and cytometers
WEHI's cell sorters differ in their capabilities in a number of ways. There are different laser configurations, different detector arrays, and all have the ability to sort into microtitre plate wells. The MoFlo, DiVa and Arias can sort 4 populations simultaneously and also sort 4 or more times faster than conventional sorters. The sorters are calibrated to give similar results for most fluorochromes wherever possible. Cytometry lab staff will allocate a suitable instrument when you request sorting time. If you have a preference for a particular sorter for good technical reasons, cite them in your request. See also the sorter technical comparison chart or information on common fluorochrome combinations.
It is important to be familiar with the procedures regarding biohazardous or potentially biohazardous samples.
Before beginning a new series of experiments, discuss your plans with cytometry lab staff.
To reserve sorter time, go to the online schedule or fill out a request form available in the Cytometry lab. Each Monday morning, the schedule opens for the week beginning 14 days ahead. Check the schedule online for time that may become available through cancellations. Empty slots may be requested but requests entered on line will not be processed until the next business day. External users must arrange time in person and may book only 6 days or less in advance.
Reserving analytical cytometer time is instantaneous. Go to the online scheduler and click on the time required. An internal phone number and cost centre code are required.
Rules for Cytometer Use
- Enter your name on the schedule before using the cytometer.
- If you use the cytometer you are responsible for shutting it down. DON'T JUST WALK AWAY AND LEAVE IT RUNNING.
- Only if you can talk to the next scheduled user in person, and they confirm they are coming, may you leave the cytometer running (less than 3 hrs in "standby" mode).
- If your name is on the cytometer schedule you are responsible for shutting the cytometer down.
- If you run biohazardous material in the cytometer, you must thoroughly decontaminate before leaving.
- If you allow the cytometer to run dry of saline, you are responsible for removing air bubbles from the saline lines.
- If you leave data on the cytometer's local hard disk, expect it to be gone when you return.