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© Copyright 2006
Walter & Eliza Hall Institute
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Local Time @ WEHI:
06:23AM on Tuesday, December 2, 2008.
Free Flow Cytometry Software
This software has been developed and used at WEHI and is provided in a spirit of collegiality for testing by interested parties. It is provided without warranty of any kind and no claims are made regarding its suitability for any task. All are Java programs and will run on systems with Java 1.4.2 or later installed. Unpack the downloaded file to extract the application and launch by double-clicking.
!WARNING: Do not subject primary data to any of these programs. All files should be backed up prior to processing.
The Cellular Symphony:
The Cellular Symphony is an audio representation of flow cytometric data. Each cell is represented by musical notes of timbre, pitch and volume dependent on its FCM parameters. The program correlates the audio output with 2D and 3D graphical display. It may be that only a musical savant can detect the intricacies in a 3-instrument cellular symphony or it may be that the average cytometrist also can do so with practice. Your comments are invited.
- Download Cellular Symphony for Macintosh [114,173 bytes].
- Download Cellular Symphony for other systems [429,226 bytes].
A description of The Cellular Symphony was presented at a meeting of the Australasian Flow Cytometry Group (see AFCG 2006).
Note: in addition to Java, CellSymphony requires an accessible midi synthesizer (supplied with most modern sound cards).
De-Identifier:
Where flow cytometry data files from the clinic are to be used in research projects, DeID is used to remove patient identification. This is achieved by writing a copy of the data file with selected keyword values removed.
Hackit:
Where it is feared that a flow cytometry data file has been violated, for example at the start of the file by cells carried over from the previous sample, or at the end of the file by a nozzle blockage, Hackit can be used to clip the offending stream of cells from the file. It may be used also to concatenate the data from a number of files.
Weasel:
WEASEL is a general purpose flow cytometry data analysis and display program developed at WEHI. It provides many different display formats and the extraction of a number of numerical or statistical measures. Multiple data files can be compared side-by-side on screen and there is facility for batch processing. Display layouts may be saved as may high quality graphics which may be incorporated into other documents. One of the program's design parameters was to make operation as simple and intuitive as possible.
WEASEL is not provided free but a fully functional copy may be downloaded free for testing (for further information on the latest version see WEASELv2) .