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The laboratory has been providing a cell sorting service at WEHI since 1977, with the introduction of the ‘FACS II’ which was the first cell sorter operated in the southern hemisphere. The ‘FACS II’ was still running (in greatly modified form) up to its retirement and replacement in 2002. The Cytometry laboratory currently runs 5 cell-sorters of a variety of types. For do-it-yourself flow cytometric analyses, we maintain 7 cytometers, also of a wide variety of types. 

New Users:

Those new to flow cytometry may read the basic introduction to cytometry. New or potential users of the cell sorting service or the analytical cytometers should look at the new users information and cytometer rules, for brief comparisons of the instruments, and may review the instructions on reserving sorter or analyser time. For potential users of the analytical instruments, a CD tutorial is provided that describes how the FACScans operate. Introductory training for these and the more sophisticated cytometers and their software can be arranged with Cytometry lab staff on an ad hoc basis.

Advanced Users:

More experienced users who are considering more sophisticated or less common fluorescent staining of cells might first consult "Fluorescence in Cytometry" to review the basic principles and should then check the feasibility of the experiment using the fluorescence plotting program FCMDesigner.

Biohazards:

It is most important to be familiar with the procedures regarding biohazardous or potentially biohazardous samples.

Charges:

Visit the user pays billing page for details of the cost to your laboratory of using the sorters or cytometers.

Data Preservation:

Data acquired from the sorters will be stored for each user in their K-Share server FACS_DATA area.  Users of the analytical cytometers are responsible for their own data preservation.  See the data storage and analysis section for more information. K-Shared flow cytometry data may be analysed and displayed using WEHI's in-house analysis and display program WEASEL.

Utility Software:

Several utility programs used at WEHI for flow cytometry data operations are available for public download. See free software.

Quick Links:

• Introduction to Flow Cytometry; Background info on the technology.
• New Users Information; Info on using the WEHI facility.
• How to Reserve Sorter or Analyser time [or go directly to the on-line schedules]:
  • Analysers on line (DIY operation).
  • Cell Sorters on line (Cytometry lab staff operation).
• Cell Sorter Technical Comparison; compares capabilities of WEHI's sorters.
• Fluorochrome Combinations; combinations suited to WEHI's cytometers.
• FCMDesigner; Fluorochrome and optical filter spectrum plotter.
• Fluorescence in Cytometry; Guidelines for choosing fluorochrome combinations.
• Exciting Fluorescence; Semi-classical view of the physics of fluorescence.
• Biohazards procedures
• WEASEL; Data analysis and display.
• Data Storage and Analysis.
• Billing.
• Example results
  • BFP/GFP
  • APC-Cy7
  • UV and 488 Lasers on MoFlo
  • Quantum Dot 655 Conjugation
• Recent Presentations
• Cytometry lab flow cytometry publications
• Free Flow Cytometry Software
• Other useful links

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