Cytometry Lab

Confocal Laser Scanning Microscopy (CLSM)

The Cytometry laboratory at the WEHI is equipped with a Confocal Laser Scanning Microscope (CLSM). Confocal microscopy is a relatively new technique that permits us to obtain high-resolution images of very thin sections of a specimen. This unique optical sectioning property, together with a suitable computer, allows us to image structures in three dimensions. Specimens to be imaged with the confocal must be labelled with a fluorescent probe.

Confocal Microscopy training sessions are available for new users by appointment. Both training sessions and bookings can be made with the Cytometry staff on 9345 2540.

 

An overview of Confocal Microscopy

The WEHI Confocal Microscopy system

Preparation of samples for CLSM

Colocalisation in Confocal Microscopy

Confocal Microscopy at the WEHI (powerpoint presentation, works best with Microsoft internet explorer)

How to use the Leica TCS-SP2 (powerpoint presentation, works best with Microsoft internet explorer)

Useful CLSM Links