Cytometry Lab

The WEHI Confocal Microscopy system

The Confocal Microscope Facility at the WEHI consists of a Leica TCS4 SP2 spectral confocal scanner equipped with 4 lasers and attached to a Leica DMIRE2 fully motorized inverted microscope.

Laser	Wavelength/s (lnm)	Dye
Multi line Ar UV laser	351, 364	DAPI, Indo-1
Multi line Ar laser	458, 476, 488, 514	FITC, Alexa 488
Green He/Ne	543	TRITC (Rhodamine), Alexa 546, Cy-3
Red He/Ne	633	Cy-5, Alexa 633

Table 1. Lasers available on the Leica TCS4 SP2 at WEHI and some common dyes that can be used with this unit.

For more information on the confocal system go to Leica microsystems.

This unit is also equipped with a live-cell incubation chamber (Helmut Saur/Leica POC-Incubator) which has both temperature and gas regulation capabilities.