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WEHI @ AFCG2007

Welcome to participants in the 2007 annual meeting of the Australasian Flow Cytometry Group Inc. The following presentations were delivered to the conference and to the preceding flow cytometry workshop respectively:

Characterising the Cyton: Modeling Lymphocyte Proliferation and Survival:

Edwin D. Hawkins, Mirja Hommel, Marian L. Turner, Francis L. Battye, John F. Markham and Phil D. Hodgkin.

A model of the proliferating lymphocyte, built on an internal mechanistic unit called the cyton, has been developed and implemented as a computer program called CytonCalculator. This program allows user adjustment of the model parameters while graphically comparing the model prediction with experimental proliferating cell numbers per division state at multiple time points.  These numbers are extracted from flow cytometry data using the Weasel flow cytometry data analysis program. CytonCalculator can also seek a best fit to the data by using Levenberg Marquardt fitting methods. [Abstract]. 

The full presentation is available here: (Presentation: 1,674,564 bytes).

For further information on measuring lymphocyte proliferation, click here.

For operating instructions and download of the CytonCalculator, click here.

For description and download instructions for the Weasel program, click here.

Principles of Flow Cytometry and Cell Sorting: Revealing the Identity, Function and History of Biological Cells:

Francis L. Battye. [Abstract] | [Presentation: 1,461,742 bytes]