';Aggcctcaacaaggaccgag@Taattgtatccgtcattcat@;2710Acctgtttat@Agcaaggtaaaactccgctg@xAgtttttataattggattga@zAttattatcatcatatatgt@Ggagaagaat@Tattaaattt@Tgtatttattcctgcagcaa@ Ä#sOGeneQS@FJ? @ @y@f) @09L!h@r@V(@?D<B/ 0`B8@1C2E3G4I5K6M7B9FHBAM2001MAY84@w Working WEHI STSs:*0002f12--0055:12--0055:12-000412-000512-000612-000712-0008 12-0009 12-0010 12-0011 12-0012 12-001312-001412-001512-001612-001712-001812-001912-002012-002112-002212-002312-002512-002612-002712-002812-002912-0030 12-0031!12-0032"12-0033#12-0034$12-0035%12-0036'12-0037(12-0038)12-0039*12-0040+12-0041,12-0042-JGnJ(x(t US Letter A4 LetterUS LegalInternational FanfoldComputer PaperEnvelope (#10)els^23456789 123456789 123456789 123456789 123456789 123456789 123456789 123456789 123456789 12345 A BsMain Dictionary G< L15M,,./: AM PMX{ 2/25/97@ ALTERNATIVE NAMESAPPROXIMATE LOCATIONCHROMOSOME ASSIGNMENT COLUMNCOMMENTS DATE OF ENTRY DATE OF MODIFICATION FRAGMENT LOCATION GENBANK IDISOLATE LAB YAC NAME LOCUSPCR PRODUCT SIZEPRIMER AF ENTRY DATE OF MODIFICATION FRAGMENT LOCATION GENBANK IDISOLATE LAB YAC NAME LOCUSPCR PRODUCT SIZEPRIMER A PRIMER BPROBE DEFINITION PROBE NAMEPROBE SEQUENCE PROBE SIZE PROVISIONALPUBLICREACTION CONDITIONSROW SERIAL NUMBERSTATUSSTS WELL LOCATIONYAC INSERT SIZE YAC LIBRARY YAC NUMBER YAC POOL                D ASTSBG APrimer aBAPrimer bBALocusBAAlternative namesBAPCR Product SizeBA Probe NameBAProbe SequenceB A Yac NumberBG r  AChromosome AssignmentB ACommentsB A Date of EntryBG  ADate of ModificationBG A Serial NumberBG L120 A Well LocationBAYac PoolBG  NAFragment LocationBA Yac LibraryBG   A23D7 MunI Tony Triglia size selected 3D7 R1 FCR3 A42AReaction ConditionsBG  A941.00', 55 1.00', 72 1.00', 35 cycles 941.00', 45 1.00', 72 1.00', 35 cycles 94 1.00, 50 1.00, 72 1.00, 35 cycles Other- see comments AProbe DefinitionBG     A7Anonymous marker Known gene sequence cDNA clone YAC endh7AIsolateBG   A B8 3D7 FCR3S A Probe sizeBAStatusBG  A'Mapped Unmapped Sequenced Non STS Probe'APublicBG  AYes NohA ProvisionalBG  AProvisional FinalhAApproximate LocationBG  ALeft Middle RighthAYac Insert sizeBG r A Genbank IDBARow  BG r AColumnBG r@  A Lab Yac NameBG r @@@XARecord@@AControl Screen@@AChromosome Listing@@A STS Listing@@A Probe Listing@@ANotes@@A Record CopyARecordB @"A     B     C     D       E     F@    A$,.#YesfNoG     H   O  I@    A$,.#YesfNoJ@    A$,.#YesfNoK   O  M     N*    O*      P@    A$,.#YesfNoQ     R     T     U     V     W@    A$,.#YesfNoX     Y      Z      ]       ^@    A$,.#YesfNo_     `     a@    A$,.#YesfNob     A&P $ʸ79E6P9G7ʸH9V6PIW7ʸ!(/%P#1'ʸ!*/P   B%ʸ8FP:5Hʸb'P(Jʸ+`P+ QXʸzJP IkyDC ʸN sP I O ʸ`P B ʸzq ,,ksy ,Z`l+M3   DYl(+ 3l+3 h,,s ,Z   V gE   pC  p  pN  p, p!pp p  F p    zE,,kyD,ZY}gz+Ygs3)',,()G,ZzDr,,kHyo,Z'|I,(,Z,,[,Z F, ,    H``zIIkJy ^\!f:z ky"f:z!II`ky#f:z"ky$f:9GP%m@%C   ESTSC   EPrimer aC   EPrimer bC   ELocusC     EAlternative namesC   EPCR Product SizeC   E Probe NameC   EProbe or STS Sequence C   E Yac Number C   E Chromosome C   EComments C   EYac PoolC   E Date of EntryC   EModification DateC   E Serial NumberC   EFragment LocationC   EMenuC   E Yac Library  C   EReaction ConditionsC   E DefinitionC   EIsolateC   ESizeC   EStatusC   EPublic C   E Insert size!C   E Genbank ID"C   ERow#C   EColumn$C   E Lab Yac Name%C   Ebp @B) Bv)    A  !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIBw)        /13579;=>?ACE GABCDEFGHIJKM NOPQR T U  V WXYZ]^_` ab"""A"""""  "1 "/ "5 " "C"E""?"G""">" "7"3"""""9"=";"/ "5!"""C#"E$"%"?&"G'"/("5)"*"C+"E,"-"?."G  /"/0"51"2"C3"E4"5"?6"G7"/8"59":"C;"E<"="?>"G?"/@"5A"B"CC"ED"E"?F"GG"/H"5I"J"CK"EL"M"?N"GO"/P"5Q"R"CS"ET"U"?V"GAControl ScreenB @  A '`<i `'a<[Qc?XR`Kzi `<i`'*sLc `'dQBfRc} c`'LY6yV p8xA 7BnAyV BY6yVhY6yV   C}L@QL f L f>PAf ; LL %g 6Dc `'6UQ9RREc 6c`'} `'QR  E `'@ `'2Q/R"@ @`'IFW  ֐Ww nv 7Blw BWwhWw }PW ֐-M   Gl 0M8-J85F-M X@C E-Wellcome Foundation Malaria Sequence DatabaseC  E Add an EntryC  EEdit an Existing EntryC  EExport Data to Excel C  EData entry and Editing C  ESearch for an Entry C  ERecord Manipulation C  E View Data   C  EChromosome OrderC  ESTSC  E Probe NameC  ESend an update to RossC  EHELP @BP˲BP˲Ar  !"#$%&'()*+,-./012345678rAChromosome ListingB  @A@    A$,.#YesfNo  #B     D     A/=[, ,? Pv33,?wPl+0q>3,?PF+0>3,' # V"g  B' 'pC%&p &pN$%p,&p!pp&pCT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenu A* UU  UU* @B>3333@A$ $B>3333@ABQ3333@ A STS ListingB @A@    A$,.#YesfNoB     D       A0>!, ,?PB33,?Pjl+0>3?lP+0f>3,' # V"gB' 'pC%&p &pN$%p,&p!pp&p  CT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenuA* UU  UU* @B>3333@A$ $B>3333@ABQ3333@  ! A Probe ListingB @A@    A$,.#YesfNoB     D     A0>, ,?P@33,?qPl+0p>3?Pp+0>H3' # V"g  " B' 'pC%&p &pN$%p,&p!pp&pCT$p' ' @C   E ChromosomeC   ESTSC   E Probe NameC   AMenu !#*A* UU  UU* @B>3333@A$ $B>3333@ABQ3333@ ANotesB  "$ AP  Vg pCp pNp,Bp!pppp  @C   #% ASTS- Nomenclature is of the form X00-0000 X is single letter lab designation I- Institute of Molecular Medicine W-WEHI F-Florida M-Menzies R- NIMR S- Sloan Kettering First two numbers are chromosome number UsBe 00 if not assigned to a chromosome Next four digits is STS number Nomenclature for corresponding primers X00-0000y where y is either a or b corresponding to primers in the listC   EMenu @B[d^ $&  B[d^A   B[d^A Record CopyB @"A     D     I@    A$,.#YesfNoM     R     V     ^@    A$,.#YesfNo %' e`      a@    A$,.#YesfNob      A&P $ʸP)NP I(H ʸ)u ,,w( ,Z)I,, (H &( B,Z)Hv,,L(s,Z)MIN( ) ("f:)!I(#f:)C$"($f:m@%C   ESTS C   E Yac Number C   EYac PoolC   E Yac LibraryC   EIsolate C   E Insert size"C    ') "ERow#C   EColumn$C   E Lab Yac Name @B) B) A& &B)      ADIM R V ^` ab                    (R            ! " # $ % & ' ( ) * + , - . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I AW 3-00081/03/96 2A>    >TAnonymous markerU3D7V300 bp[Middle^F_8s' A gA gA gA gA gA gA  g  g g g g g g g g  g  g  g  g  g g g g g g g g g'C   g g g g g g  g     @ Find New entryTestFind all/Control ScreenChromosome sort STS Sort Probe SortUpdate for Ross AFind. BHH +6G{HH d'@E7 Y*``QA New entryE7 (QATestEQAFind all/Control ScreenBHH%.7@G{HH%.d'@E7-/ { (*`QlAChromosome sortEL157'` (> e ? QlASTS SortEL157 '`>  ?QA Probe SortEL157 '`>  ?Q AUpdate for Ross.0  BHH +6G{HH d'@EI7 Hzr*`@ $@@XYes                    />               d g g g  gQBUUXcM  A$,.#YesfNoA : AM PM@ HelveticaGenevaCourierTimesArial@`823KAPICT *A *  $HHff33̙ff33Bff33ffffffffffff33ff33333333ff333333ff3313 Cff33̙ff33̙̙̙̙ff̙33̙ffffffffffff33ff33333333ffD333333ff33ff33̙ff33ff3324 Eҙffffffffffff33ff33333333ff333333ff33ffffffffffff33ffffffffF̙ffffff33ffffffffffffff33ffffffffffffffffffffffff33ffffff33ff33ff33ff33ffff3333ff33ffffffffffff35 G33ff33333333ff333333333333̙33ff33333333333333ff33333333ff33ff33ff33ffff33ff3333ff3333H333333333333ff333333333333333333ff333333ff33̙ff3346 Iff33ffffffffffff33ff33333333ff333333ff33wwUUJDD""wwUUDD""wwUUDD""57 KһwwwwwwUUUUUUDDDDDD""""""   L            69 YMR  3 *A`PICTAFF FF FF"% q% ?0% 4?1 "0 #### ###  >1 `7: BFF "+ "D">dMDPLk UUUUq.1D1>D DD=851dMDPL  C"1 ############### UUUU  FF # #######"2  "1#9= D FF#"* dMDPL}/ wwwwq> :3&* . 0539:88 3%&V """"q> :3&* . 0539:E88 3%&dMDPL  "&####################### """"  Y12-0001@12-0043@12-0091@HW@[Acagtttggaaacataaata@ gaagacaa@Ggtcatggtgatgataacaa@Ttattcaagcgatgtaggaa@ Ttatttaaatcttcaagtaa@kAagattagggtattatggtt@Agtaccttctacactctcct@Catgatgactgctatctacc@Taattgtatccgtcattcat@Tatactttccatgcttctct@Tccctatagatgattcttgt@_TctttttcttcttcctctatTttttccatgttatttattg@Ã"@Ã4P3a9f@4b4fFX6a9f@Aatgcatcatttcataatat@Agtttttataattggattga? CD:F=A@AACBECGDIEKF:@ FFF ############"%  "&# FF"&")" ,"@  q<D <G@ D< "<#####  FF ###"1"% "? 3A(PICT08(ځ`V< K(@>`B8@1C2E3G4I5K6M7B9=A -A09 ~8  H I@B%/<@p@? R Q Q B&PP`p`3A3KPICT @B A   HH"Lff33̙ ff 33 Bҙ ff33ffffffffffff33ff33333333ff333333 !ff"33#$%&AC Cҙ'ff(33)*+,̙-ff.33/0̙1̙2̙3̙ff4̙335̙6ff7ff8ff9ffff:ff33;ff<33=33>33?33ff@3333DA33BCDEffF33GHIJKffL33MNOP̙QffR33STUVWffX33YZff[BD Eҙff\ff]ffff^ff33_ff`33a33b33c33ffd3333e33fghiffj33klffmffnffoffffpff33qffrffsfftff̙uffFffvff33wffxffyffzff{ffff|ff33}ff~ffffffffffffffffffffff33ffffff33ff33ff33ff33ffff3333ff33ffffffffffff33ffCE G33333333ff333333333333̙33ff33333333333333ff33333333ff33ff33ff33ffff33ff3333ff33333333H33333333ff333333333333333333ff333333ff33̙ff33ffDF I33ffffffffffff33ff33333333ff333333ff33ٻڪۈwwUUDDJ""wwUUDD""wwUUDD""E KҪwwwwwwUUUUUUDDDDDD""""""   L   3 NProvisionalomInOrderedOverlapPrimersSequencedSequences()*+,-./012356 Cs :User Dictionary!&@D2F6F&dehlopÁ@fgijkmnqrstuvwÂ#  !"#$%'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bcă#`a017W12-0018W12-0019W12-0020W12-0021W12-0022W12-0023W12-0025W12-0026W12-0027W12-0028W12-0029W12-0030 W12-0031!W12-0032"2f?tMA44oC annealing@A>    >TAnonymous markerU3D73D73D73D7B8V296bpWcompleteXYesYFinal[Left\75606560190^GBBAE_85575A W 12-0004BAAATAAACTCGTTTTTCTCATTACAAACGTATATTCAAGTAAAGTCG F107G8G3FI341 313 717J12L21/03/96M27/03/96N4P448R3D7 MunI3D7 MunI3D7 MunIS43oC annealingTAnonymous markerU3D73D73D7D7 MunIFEPro 2.0F!\@\@C2(H Pro 2.0 - 2.1I-w @‚69:;<=>^_‚G-‚QMNOP‚Sfg‚z-./0a‚‚/01‚ Ã"PfÃ# 34567Ã#PÃ$stÃ&PkÃ&p]^Ã(fÃ) JKLMNOPQRSÃ)`jÃ10HIÃ4Ã4Ã5@-Ã6PjÃ6Ã7pIÃ8P?@Ã:@789:;<=Ã:PmÃAkÃA ÃA`567ÃB0VWÃB@ ÃCkÃC\]^ÃDpSTUVÃZ`%'(Ãa 'Ãd !"#Ãe./012Ãi0+,-.ÃiBCDEÃjP$%Ãq@01ÃrPCDEF0\A W 12-0001BATATCGTAGAATGTTTGCTTGAA CTGTGGTGGTGGTTTTGGTAGTTG D2F6FF196G2F6FATTGTACTGTCGCAGGAGCTCAGAAAATGGAGTTACTAACAAAAAGGATATCGTAGAATGTTTGCTTGAAAATCTTAAAACAAAAGCAAAAACTTGTCCAAACCAAGTTAATGGCGAAAACCAAACGTGTGATTCCCTCCCTCATGTTGAAGACGATGACGATGAGGAACCCCTTGAAGAAACAGAAGAAAATACTGTGGAACAACCGAACBoATTTGTCCGCAACTACCAAAACCACCACCACAACCAGGGGATGAAGACGGTTGTAAACAGGCATCACCAGCACCTAGTGAAGGCACAGAGAACCAACCCCCTGTGATCAAGA@A I349 1262J12Homology with var-31ITWcompleteXYesY Provisional[Left\19013565^ECD_519A W 12-0005BTGGAGAAAAGAAATTAGGAAATA CGATCCAATATACCATCAGCACTT F104G4B4FAATGTAATAATCATGGAGAAAAGAAATTAGGAAATATATTAGAAGTATCTCATGATAATGTATCGAATGAATTGCCGAGAATGATATCTGAAAAAAGTGCTGATGGTATATTGGATCAACTGTTTGATGAACAATCCCTAAAGACATCAAATACCTTAAGTAGTGATATAATGGGAGAAGTAAATGAT @A I341 717J12L21/03/96M27/03/96N5P48R3D7 MunI3D7 MunIA45oC annealing5X Ä#sÄ#w0Ä# Ä#()*Ä#*Ä$1_Ä$XÄ$?@AÄ$KLMÄ%STÄ%#fgiÄ!mrs @fgijkmnqrstuvw‚#  !"#$%'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bc8BeBeenBelieveButByCdna)ChainChromosomeClone)ComplementConfusionControlDecemberQ Y Ä#s ‚# 1i1995AndKChromosomenDecember]F@%In3d7@Mun1Reverse@vSexualCX625400006@2X766430001@*Yac@p ‚U@d123_3d7BMuniSize45ocX?ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_fgjknqruvwSequence$%6@bcimtYacs3d7  !"#$%'()*+-./0123456789:;<=?@ABCDEHIJKLMNOPQRSTUVWYZ[\]^_`abcfgijkmnq +,-.9:;OS +Gene3d7sB8|ÃC @RPRIMER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALPIAMH@* A+ ,B-. B/ 0(3qÃC [ÃCPYÃC`ÃC ÃD?VÃD ÃD=ÃE ^ÃEP.ÃF>ÃFp*ÃG(ÃG,ÃH0XÃH!ÃI@nÃI`ÃJP ÃQ09ÃQ`CÃRNÃR@ÃR`5ÃSGÃUP8ÃV#ÃY Complete  !"#$%'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRST !"#$%'()*+,-./013456789:;<=>?@ABCDEFGHIJKLMNOPQRSTQJCompleteU3YesMiddlepÃ!N"Ã(@ER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALK@x^At@IWM@TBUBWTj Ji AZMU @A>    >TAnonymous markerU3D73D7V187bpWcompleteXYesYFinal[Left\19065^ED_59A W 12-0006BTTTCCTTTATCACCAAACCTGTT CGACTGTGTCTTTTTCATCTGAGG F228G1E9FAACAATCTTTTTTATAACATCATTATACTTGTATCTCTCAATGGTCATCTTCTTTCCTTTATCACCAAACCTGTTTTCACCATTTTTTACATTTTGTTCAACATCCACGTTTTCGTTTTTTTCTTCATACATATCATCAACTCCTATTTCATCTAACAATTTCTTCATATCCATTTTAACAAAACCTGTGGGCTTTCTTGTCCTTACAGGTD7Y Provisional[Left\190nITWBCTTGGCATATACAATGAAGCAGGTTGCTCAATACATATATGCTCTTCCTCAGATGAAAAAGACACAGTCCTTTTTACTACGTCACTATCTATATTCTTCCTTTTAAAAGTTACCTGTTTCTTCTTTTCATCATCATTCACATCCTT  e@A  I341 313J12L21/03/96M27/03/96N6P44R3D7 MunI3D7 MunIA49oC annealing@A>    >TAnonymous markerU3D73D7V356 bpWcompleteXYesYFinal[Left\190135^EC_51A W 12-0007ft\190135?sH @& @sA+ ', `B-. B/ 0(. B/ 0((((0(((0((((((UBATATTACAAGGTTTAGGAGGTATCTCATTATTATTCGTTGAAGGTATF176G2E4RATTTTATTTATTATTTTATATTACAAGGTTTAGGAGGTATATTCCTTGGGTTTTTTTCAGATATATATGGAAGAAAAATATGTTTAAATATATCCTTTTTTATTTTATTAATAATATTTATTCTTTCTTTTATTACGGTGAAAACATCTTTCTTTTTGTTTTATCAAAACATACCTTCAACGAATAATAATGAGAAGGGCCTGGATTTATTB.CTTTTATACTAATACACCTCATACACAAATAAATGCATATCATGAA@A I341 313 95J12L21/03/96M14/04/96N7P441R3D7 MunI3D7 MunITony Triglia size selectedS43oC annealing 4b4f4f6rP4h7rF5'k1cl12b55k5a11f 5b11fV5b12fJ5b1fS5c11f35c12f5d9f5e11f5e8f^5f11fB5f12f"5h11fK5h12f-5h1fX60@6a2f?6a4f>7e12r:8a11r8b10f,8d7f8e6f8e7f 8g2r48g3f8h4f0Abra6Atp2%Atp4Clat$OraAaaaaagaatattgaaagta7Aaaagtttataaaaatagat Aaaatacgaccacacaaatg4AaagaaattgaaaaaataatAaagagttaaattagttataAaatgtaaacacattaggaa Aatatctttataacattttg"Aatgaaaaatatgatgaagaac I1271 492J12L21/03/96M27/03/96N13P146R3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D7V282WcompleteXYesYFinal[Left\60225^BA_1112A W 12-0013BATATTCATACAATTTCTGAGCCAGTATGTAGAAACTGTGTACTG F800G1H11I1271 946 492J12L21/03/96M27/03/96N13P14106R3D7 MunI3D7 MunI3D7 MunIS50oC annealingTAnonymous markerU3D73D73D792h`  A>    >TAnonymous markerU3D7B8V262 bpWcompleteXYesYFinal[Left\135120^CH_111 A W 12-0010BACTGCGCTTAAATAATAATCACAA CTCAAATAATCTATACTACCGCTGTTF230G5A11FAAAATGTAAACACATTAGGAAGGGAAAATAACCCTTCTCTATTCTCATCAAGAAATGAACAACTGCGCTTAAATAATAATCACAACATCATGAATAATAACAACACCAACAACAATAATAATAATAACAACAATAATAATAATAACAACAATAATAATAATAATAACAATAATAATGGAAATAATAATAACCATTGTAGTCATGGAGATACI341 313J12 AvAaafijAaaatacttattaggttcctZAaaattaaataaccgtggac;Aaactaacaacgaaaatgct2AaataaactcgtttttctcaAaataaatgttggtatggtgHAaatatgaatatggatactaVAaatcaaatattgtttcaaa!AaattagttatagagaaaagAaatttaaatattgaagaagAacvAagkuAagcgactcgttgtgtaaaa>AatgAatataatagagaacatctt_Aatatcagtgggaaaatgtg=AatattttctgatatttcatXAattataggacacttcgttcAattcttgtgcttcatctaaRAcarvAcaattagtggaaataaaacGAgcaaggtaaaactccgctg?@JKLMNOPQRS^_0CDEFGHIJK[`fgijn@%'(-01?@AXYZ[P !"#$%MNOPku` )*56789:;<=\]^_jmqp !"#$%'()*+,-.0KLMTUVafgjmnrs !"#$'(/01345./0123456IJKLSTUVfgi"#$'-./0STYZaijn‚! !"#()*+./0?@HIkst`g CCGGTATAATTAATAAATGAAATAF248G6D11FAAAAAGTTTATAAAAATAGATGTGATGGTGTATGTGTATTAAATGATAATATATATATTTGGGATAAAATAAAAAAATGTATATATTTGTATACACCATATAATAATGTATGGTATATCTTTTTAAATATATATGAAGAAATGTCTCTAAGAAAAAATACACTTTGGTCTTTATGTAGTAGTGATATTAAAAATGATATTAATGTAAATATBvAAATAAATCATATTATAAATATCATAATAATATTTCATTTATTAATTATACCGACATGGTCTACTTAAACAATTCTTTATTTATTATAAGTAGGAATGCACATTTTATGAATATTTG H@A I95J12L21/03/96M14/04/96N11P1RTony Triglia size selectedTGTGTAT 4xtN@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesY Provisional[Left\75606560190^GBBAE_85575A W 12-0003BACAGTTTGGAAACATAAATAAGTCAGTACCTTCTACACTCTCCTTTTF157G6B1F12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@nonymous markerU3D7Tccctatagatgattcttgt TccttgtttaaactcctcatLTcgtTctacattcatctcatatttITcttcctccgtttcttgatt6Tctttttcttcttcctctat actcatttttagtTttttccatgttatttattg2f6f4AtpaseOra‚‚Ã!0Ã!PÃ!Ã"` Ã#pÃ$ Ã$@Ã% Ã% Ã&pÃ&Ã(Ã(pÃ)`Ã)pÃ*pÃ3Ã3Ã4 Ã4Ã5 ÃC0ÃGÃ1b12f1d11r1e1f 1e9f1h112a7f2c10r2e4r2e7f 2f6f2g9r3a8r4b4fZ] BCTACTTTTATAACTATCTACGCTATAGTGTTAATAACAATGGACGTAACCATAACAACAGCGGTAGTATAGATTATTTGAATGATAACGAAGAAAGTGATAACAAATAATAATAATAATAATAATAATAATAGTAGTACTAAAGAAAGAAACAAATAGTAGATC w@A I95J12L21/03/96M14/04/96N10P1RTony Triglia size selectedA46oC annealing@A>    >TAnonymous markerUB8V394 bpWcompleteXYesYFinal[Left\120^H_11 A W 12-0011BTAGATGTGATGGTGTATGTGTAT gY A W 12-0012BTTATTTAAATCTTCAAGTAATTCCAAAGAAGCAAATTATTGAATGTG F147G6H3FAACATCATCTAATAATTTCATTTTTTCCATTTCTAATTCTTTTCTCTTTTTATTTAAATCTTCAAGTAATTCATTATATCTATCCATTCTACTATTTAATTCTTTTGATTTTATTAAGTTATTTCGTTCATTTCTTTGTATTTCTTCTTCTTTGTTTTGTAGATTATTATATTCACATTCAATAATTTGGTTCTTTTCTTTTTCTAAAGATBITCTAATTCTTTTTTCAACATTTCCAATTCATTCTTTTCTTTCATATTATTCAATTTCTCTTCTTCTAACTCT  @A  CTTTCATATTATTCAATTTCTCTTCTTCTAACTCT  @A  nK1. YAC 620 is a multitransformant 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M3/10/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V213 bpYeWcompleteXYesY Provisional[Left\6075225^BGA_111012A W 12-0014BAGAACATTTTATATACACATATGCGATCTTAAGATATAAACATTCG F131G5C12FAAAAGAAATTGAAAAAATAATAGAACATTTTATATACACATATGAAAAACCAATTTTTAAATATTTAAAATGCAAAAAGGTAGATAACGTAAATATATTTTTTTCTTATCGTCTAAATAATAAAAACAAACGAAATGTTTATATCTTAAGATCTATATGTATATATATTATTGAAAGTCAATGTTTCAAA @A I1271 946 492J12L21/03/96M27/03/96N14P14106R3D7 MunI3D7 MunI3D7 MunIH1 -./019:;<=>MNOP^_afg'()*+13'()*+-14 ()*0153-4 5 !"#)*6  !"#%'(./7 !"#$%+,-.8 !"#$%9 !"#$%./3d7  !"#$%'()*+-./0Muni  !"#$%'()*+-./0cl2A45oC annealing@A>    >TAnonymous markerUB83D73D7V189 bpWcompleteXYesYFinal[Left\6075225^BGA_111012A W 12-0015BATTGAAAAAGCAAAGAGAAAAAT CGATTGGTGTGATGGTTGATGTTCF186G7D8RI1271 946 492J12L21/03/96M27/03/96N14P14106R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@I3D7 MunIA45oC annealing@yK@r wAuAz[{A@|MA@PBAK@AK^@AAAR A!BIo"@#AA$%&@'K(AR)A )A *@A+A,B-B A.B B/@CK01AA2A]a[ S42oC annealingTAnonymous markerUB8V327 bpWcompleteXYesYFinal[Left\120^H_11AATATATATATTTGGGATAAAATAAAAAAATGTATATATTTGTATACACCATATAATAATGTATGGTATATCTTTTTAAATATATATGAAGAAATGTCTCTAAGAAAAAATACACTTTGGTCTTTATGTAGTAGTGATATTAAAAATGATATTAATGTAAATATBvAAATAAATCATATTATAAATATCATAATAATATTTCATTTATTAATTATACCGACATGGTCTACTTAAACAATTCTTTATTTATTATAAGTAGGAATGCACATTTTATGAATATTTG H@A I95J12L21/03/96M27/03/96N11P1RTony Triglia size selectediZ ATCCGTACATTTTCTACATCTCTTCAAATGATTATATTTATCATATAATTGATTATTAATATAATCTTTCATAATATTTAAAGCGTCTTTAGCAACTTCTTTTTTTACTTCAAAACAATACATGGGAACAAGCTCCACATCTCTTGTATACGAAAAAGGTAATATTTCTTCTAAGTTTATAACTTGTCTATCCATATTCTGATAATAATTAB5AATATAGGGCTAAATATTTTTTATTCATATATTTTATATATATCATACATGT  @A  I313 95J12L21/03/96M14/04/96N9P41R3D7 MunITony Triglia size selectedA41oC annealing@jhP J12L21/03/96M27/03/96N8P44R3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D7V244 bpWcompleteXYesYFinal[Left\190135^EC_51 A W 12-0009BATTATTAATATAATCTTTCAT CAGAATATGGATAGACAAGTTA F149G1E1FA I341 313J12L21/03/96M27/03/96N8P44 I341 313J12L21/03/96M27/03/96N8P44i A W 12-0008BTAAGTATTTGTTTTATTATTACGCTCCCTATAGATGATTCTTGTTCAF115G2E7FAGAATTTCTTCCACTTTTTGTATTTTTTGTATTTTTTGTATTTTTTGAATTTCTGCTTGATTATAATATTCAAAAGAATTATCATAAGTATTTGTTTTATTATTACGTAAATTTAAACGTGTATCCATTCGATTATGTACATTTTTTTTGTTTTTTTTATTTTTTTGATTATTATATGAACAAGAATCATCTATAGGGATATTACACATATB"TACAATATTCATCAATTGAATTGATAAAAAAAAA@A I341 313TAGGGATATTACACATATB"TACAATATTCATCAATTGAATTGATAAAAAAAAA@A I341 313 Ttatttaaatcttcaagtaa Ttcctcatccttagtatcat/TtgvTtttTttagatgaaaaacaaatgg*TttatcgtacgaaatgaatgBTttcctttatcaccaaacctTttgtcctgctagaaatagaUAaairAaaaaaaattagatgatagtSAaaaacaggaaaaaagagttAaacgtatattcaagtaaagAaagaaattacattattataAaagaagcaaattattgaat AaagcactgtctaaggtatcQAaatatgttacatcatccac,AaatcttgtgcttcatctaaRAaatttaaatattgaagaagAacvAaccattttagattcatcttAAacgtatatacattcaagttJAacgttcgttcattatttcaCAagfaaatggag*eqAAATTTTTAAAAATACTCATGTCAAGAAAACATATCATACAAAAAATTATAGATAAAAATAATTTATCCACACATATGAATGAATATATCGAATATAATTATTATTTAGAATATAATAAAAAATTGAAAAAGCAAAGAGAAAAATTACTAAATCTAATTATAAATAAAAAAGGACAAATTCGAACACTTAAAAGCATAAAAAATATGTAACBmTTATGGAAGAAAATATGGTACAAGGGAAAAATAATATAAATACACATTTTGTTAAAACATTAAAGAAGTTATTAGAACATCAACCATCACACCAATCAGAAAATAACA ?@A I946 620 1092 492J12AAAATTACTAAATCTAATTATAAATAAAAAAGGACAAATTCGAACACTTAAAAGCATAAAAAATATGTAACCATTTTATATACACATATG9[Middle\150125^AC_51:A W 12--0055BCATATGATCATTTGTCAGTTTTT CTATACTTTCCATGCTTCTCTATT F114G7E12RATTTGATAAGCCATATTACTAATATCATATATATGAGCTTTCATATGTTGTTCATTAGACATATGATCATTTGTCAGTTTTTTTAAATATATATTCATTTGATATATATCATACAACCCTTTTCTCTCTTTATATGGATTAACAACTAATAATAGAGAAGCATGGAAAGTATATATTTTGTTTTTCTTATATCTGTTTTTTATATTTTGTAB-ATACATTCTCTACATTTAACTGTATAATATCTATATTGTGTTCG @A I293 25J12  >TACACATATGbrtWcompleteXYesYFinal[ Left Middle11070130225^DCDGA_81211412>TAnonymous markerU3D73D73D73D7V318 bp[ Middle Left\75110225^GDCA_1081212A W 12-0016BAGACATGCATATTCATTCTGTTGCTTTTTCCATGTTATTTATTGATAF79G2C10R0 1092 239 76 225J12KYAC 620 is a multitransformantL21/03/96M27/03/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI !6AC 620 is a multitransformant 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M27/03/96N17P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA41oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V213 bp* Yac'-./012YacsYk45f10.3C Iw}u { !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abc:dCe^fg_hijhkmlmntopuq{rstu/v Iw{  "#$%&'(/1T+lK1. YAC 620 is a multi-transformant. 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker s on this YAC L21/03/96M27/03/96N15P107126R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V318 bpWcompleteXYesYFinalnwL27/03/96M27/03/96N18A W 12-0017BTCATATAAGATGTGTAGGAGTAA CCTTATCACATTATTGGTTGAACA F227G3A8RAAATTAATATTATAGATTTGAAAGGACTAGTGACAATAGAAGTAAAATTATTAAATGGTATATCATATAAGATGTGTAGGAGTAATATCAAGATAGATGAAATATCTAGTTTTTTTGGATCTTCCAATAATTATTCTTCATATAATGATATAATAAATAAGACTATTTTATGTAACTACAATTTGAGTGTTAAGGAAGAAATTGACCTTTTBzTAATAAAAACGATAAATCATTTTTAAAACAAGATAAAGATAAAAATATTTCGAAATGTTCAACCAATAATGTGATAAGCCATAATATTATTAATAATCATTATAATAATA TAATTAGGAGGL@A I620&+ A gA gA gA gA gA gA gA g A  g A  g A  g A  g A  gA gA gA gA gA gA gA gA gA gA gA gg^IAAGAATATTGAGTTTATTAAATTTAAAACATTTCAATCATTAGTTTCATATGATAGATCTACAAAATGTTTATCTGAAAATAGTAAATATCACGTGAACTCTGATAATAACAAAGGTAATAAGTTGTTTGGACAGAAACAGTTTGGAAACATAAATAAGTGTGATGTTAAAGATGATGAATTAAAAATATCTAAAGATAATACAAACAAGABWAAAAGAAAATATGTTTTAAAAAAGAGAAAAGGTCGAATGAAGAAGAGTATAATAATTTAGAAAAGGAGAGTGTAGAAGGTACTTGT  )@A  I464 785 1073 1255 341J12L21/03/96M27/03/96N3P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIwz LA>    >TAnonymous markerU3D73D73D73D73D7V331 bpWcompleteXYesYFinal[ Left Middle11070130225^DCDGA_81211412A W 12-0018BGTGTGCTATTATGACTCTTATTA CCATTCATATTCATTCTGTTGATA F149G2A7FL27/03/96M27/03/96N20A42oC annealing@A>    >[ Left MiddleCe ReverseSSequence-.imtSequencedfwSequencesIwSequencinguSexual[/0Subunit>SurfaceSSwiss!-.:>Synthase!T-complex>T02521)T18056MThe-./0ThereIThisThree-./0To-./0IJKLMNOPQRSTTonyTrigliaTrna!TwoUCU194900001Var-3Var-7SVectorWasIWeWhereIWith!)-.:=>CMPQUWithvariant-specificSWould)*X546310001PX555040001=ru 1092 239 76 492J12 A1. YAC 620 is a multi-transformant. 2. Confusion - Oxford group have found YAC 1092 (3D7 Mun 12C12) to be from chromosome 14; we believe it to be from chromosome 12 with two additional chromosome 12 marker sBL on this YAC 3. STS sequence is reverse and complement of original sequenceL27/03/96M27/03/96N19P712316R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing@^A W 12-0002BTGCCTCTATTGTGTATACAT CTGCCTCTATTGTGTATACAT DORAF360GORAI464 785 1073 1255 341J12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing^FB_12A W 12-0002BTGCCTCTATTGTGTATACAT CTGCCTCTATTGTGTATACAT DORAF360GORAI464 785 1073 1255 341J12L21/03/96M27/03/96N2P5912144R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing-3f; @aIYWc@eAlKq[KbWnKq[eAlKq[q[lKq[Kq[AlKq[Kq[AlKq[AlKq[u{AGTTAACATTATTACTATTTTTTACTTTGATTACACCAGGAGGTGTGCTATTATGACTCTTATTAACACTATAAAAAGAGTTAATATTACTACTAGTATTATTTATAGGAATTTTTTTTTCCATGTTATTTATTGATATATCCTTATAAAGGGATATATATTTACCTTTATCAACAGAATGAATATGAATGTCTGTTGATTT @A I239 492J12L27/03/96M27/03/96N20P36R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V201 bpWcompleteXYesYFinalz[ Left Middle\70225^DA_1112A W 12-0019BACGAAACATCAAACTTTTATTAT CTTTATATACTCATTTTTAGTCAT F156G1D11RAATGACGAAACATCAAACTTTTATTATATTATACAATATGAGAATAAAGATTTTCTTACCATAATATTTATATCTCCTATAACCCTTACGACATATTACACGCTAAAAATAACTGACTTCTACTCAGGTACAAAAATATGACTAAAAATGAGTATATAAATATATATGACATGTACAAGCCATATTACATTTTGTTCGATT @A I76 492J12L27/03/96M27/03/96N21P16R3D7 MunI3D7 MunIA42oC annealing@9A W 12-0020BACATCATCTAGATCCCACACAAT CTACAATTTTTAATTCTCCTTTTT F102G5E11FATAGAGACATCATCTAGATCCCACACAATATTTACAGTAATATTAGAATCGAATCAAGTATTAAATGATGGAACATCTATTAATAAAAAAGGAGAATTAAAAATTGTAGATTTAGCTGGTAACGAAAGAGCAGGAAGAGCGGCTGAAGGGATAGAAAA@A I76 492J12L27/03/96ATCGAATCAAGTATTAAATGATGGAACATCTATTAATAAAAAAGGAGAATTAAAAATTGTAGATTTAGCTGGTAACGAAAGAGCAGGAAGAGCGGCTGAAGGGATAGAAAA@A I76 492J12L27/03/96APAnonymous !"#'()*+,-./012345789:;<=>?ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_fgjknqruvwCdna`aClone`aEndsGene$%6@bcimt\RG'()*?@IJKfgiH /01345JKLMNOPQRSjn|NM27/03/96N22P16R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V157 bpWcompleteXYesYFinal[ Left Middle\130225^GA_412A W 12-0021BAATTATAGGACACTTCGTTCAAT CACCAATTATATTCATTTTTCATA F97G8D7FL27/03/96M27/03/96N23A42oC annealing@A>    >TAnonymous marker[ Left MiddleTagagacatcatctagatccTagagattacmTagaggaagatTagatagatatTagatatatatTagatatgactTagatattattTagctcagtctTagctgggtgmTagctggttgmTaggaataagtTaggactaacmTaggagtttcmTaggattaatiTaggtaaatttTatagttaagmTatatataatmTatatggatamTatatgtgcgmTatattattamTatatttatamTatcaaaagamTatcctgaagmTatcgatcatmTatgaatcagmTatgaatgatmTatgatatcamTatgatcggamTatgatgaagmTatgcatctamTatggacaaamTatggtatatmTatgtacaaamTatgtcatacmTattagatca MuniSelected +,-.9:;<=>CDEFGHIVWXYZ[`amrsSize +,-.9:;<=>CDEFGHIVWXYZ[`amrs,743o/43oc "(18:44oc#545oc $%-2346946oc )*;47.47oc'048oc!<49oc50ocAnnealing  !"#$%'()*+,-./0123456789:;?BCDEFGHIJLMNOPQRSTUVWXYZ[\]^`Provisional%)*+0@AKbcfijkmnqrswLeft  !"#$,-.fgijnMiddle !"#$%'()*,-./)*+Ã!-Ã"Ã"`'/Ã#`./Ã$%'()*+Ã$` Ã%`AAATTATAGGACACTTCGTTCAATTGATGAACATACAGAAAAGTCTTTAAAATATTCTATGTCTATTTCAGCTAATATGAAAAATGAATATAATTGGTTAAGGTCTTTAGATACTAATGAATATGATACCAAGTGGGACGGTTTCGATAAATATGATGATCAGAATGCTAAAGAAAAA @A I76 492J12L27/03/96M14/04/96N23P16R3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D7V177 bpWcompleteXYesYFinal[ Left Middle\130225^GA W 12-0022BCAAATCTAATACATACGAATCAT CACTTGCTTTTATAACTATCAATA F185G2G9RAGGATAAAAATATTAACCATATAAAGAATGAAACTGATGCATTAAAATGTAGTAGTTATAAAAAAGAAATCAAATCTAATACATACGAATCATTTACAAACAATTCTTTAATTAATTTTGAAGATAAGAAAAGTAACGATGTTAAGAGTAATATCAGTACTTTATTATTAAATCTATATGAATATAATAATAAAAGTACAGATCAAATACABtTTATTTTGATCGAATTAAATCTATTGATAGTTATAAAAGCAAGTATAAAAATGGTTCATATTTATTGGAATTACACTCAAAAGATAGTGAAAATGATAGTAATAAAAATGATGCA F@A I492J12L27/03/96M27/03/96N2403/96N24P6R3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D7V325 bpWcompleteXYesYFinal[ Left Middle\225^A_12A W 12-0023BCAAATAAATGAAGAAGACAAAAA CCATGATGACTGCTATCTACCTTA F322G8A11RAAATGAAAAATATGATGAAGATTTTACTTTGAATTATATTCTGCAGAAATACGATGATGATGATCAATTTTTTTGTAGCAATATTTATACTCAAATAAATGAAGAAGACAAAAAAGATGAATTAAAAAAATATACAGAAGGAATGAATAGGTTAGTAGAACAAATGTACTTTTATGATAATTTCAATGATGAATATAAAATAACTAAAGAA CaaataaatgaagaagacaaCaaatctaatacatacgaatCaatatttgaatgtaatgtcWCagaatcttttccagttactMCagcttgtaaatctgttgaa5Catatgatcatttgtcagtt:CcafkuCcattggactcttggttctaCCcguCctkCcttatctccattcttgttgnCgtsCtsCtaaacagttgtgctaatacLCtactattctttctaactctaCtcrCtgtggataattcttggtccECttattaaaaattgatgagg^CttgtttataaatgtgatggDGaagGaagataacactgacgggaaYGaatataaatcgtgcttacc`GacgGaggrGatktGcakGctctatctttaataatcatAGgaagcaatataatgagtaaNu|AatgcatcatttcataatatAatggagtggmAatggctggatAatggtattaiAatgtaaagatAatgtaatgttAatgtagatgiAatgttaacatAatgttagttmAatgttcctamAattaaaaagtAattaaaatamAattaaatgttAattaaggaaiAattaataaatAattaataatmAattaatacgtAattaatattatagatttgaAattaattatattaatctaa/AattacgatgtAattagaaaatAattagctatiAattagtaattAattataggacacttcgttcAattattttgmAattcaatatttatattattRAattctttaatattataaac[AattgataccmAattggtataiAattgttatamAatttataaam%\ In3d7InhibitedUIs'./012STUVmItKb0Kinase-L406090001L426360001SLibrariesLightLikely-./0M691470001[MalariaimtMappedMarkerMore0Multi-transformantSTUVmMultitransformant'./012MunMun1-./012IJKLMNOPQRSTUVa-./0P)P31209.P37880!P39077-PcrsPf0131c)Polyadenylate-bindin.PositiveProtein!-.Regulatory-RestrictionaKNo positive YACs in3D7 Mun1 or Tony Triglia B8 YAC libraries but OK in positive control PCRs. The fragment has been mapped by restriction analysis of 3D7 gDNA.L27/03/96M27/03/96N26A45oC annealing@A>    >TAnonymous markerV148 bpXYesY Provisional[ Left MiddleGAACACTTAATTAATTTAGAA @A J12    >BAATAAAAAAGAGACAACTGAATCGTTTGATATTATAGATACATCATTGAATATACATGTTGTTGATGCTAATGAAGATAATATAACAGATGTAAACAAGGATAATCAGGAAATTATTTCAAAAATGGAATTAAAGATAAAAGAGTTGGAGAAAACTATTGATGAAATGAAAAAGAAGGATAAGGTAGATA GCAGTCATCATGA  @A  J12KNo positive YACs in3D7 Mun1 or Tony Triglia B8 YAC libraries but OK in positive control PCRs. The fragment has been mapped by restriction analysis of 3D7 gDNA.L27/03/96M27/03/96N25A46oC annealing@A W 12-0025BTCTTCCTTAAGTGATGAAAATAACA CAGCAGCAAACATTAATGACCTCGT DATPase 4F237GATP4I1347 237 357J12L27/03/96M27/03/96N27P153ysis of 3D7 gDNA.L27/03/96M27/03/96N26A45oC annealing@A>    >TAnonymous markerV148 bpXYesY Provisional[ Left MiddleA W 12-0025BTCTTCCTTAAGTGATGAAAATAACA CAGCAGCAAACATTAATGACCTCGT DATPase 4F237GATP4I1347 237 357J12L27/03/96M27/03/96N27P153 A>    >TAnonymous markerV413 bpXYesY Provisional[ Left MiddleAW12-0024BAAATTAGTTATAGAGAAAAGAGG CTTAATTAAGTGTTCTAAAACTTC F133G7A12FAAAAGAGTTAAATTAGTTATAGAGAAAAGAGGTAGAAAGGGATTAGATAAACATGAACATATTAATATATTATCTAAATTATGTGAAATAGAAAAACAATTAGTACACAATCTTATATAGAAGTTTTAGAACACTTAATTAATTTAGAA @A J12hAgtaccttctacactctcctOAgtatgtagaaactgtgtacAtaaaattattaaatcacaa8AtagatgtcgacaatgttatFAtatttttaaatcagtatgtAtgiAtgaccaccccctttttatc0AtgtttactcatcataggnAttgjsAttaatagatatgtcctgtg2Attcaagcccttcgtaatct=AtttcttctccgttatcaatBCasCaattttaaatatttcagaaqCactuCacagaaagattcataaagcGCaggruCaggcgaaagctatagttcta-Gtagtaataaaacgaaaagt/TacaatttttaattctccttTataatcatagtgtgggtaa+Tcaaataatctatactaccg Tcattattattcgttgaagga4R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D7WCOMPLETEXYesY Provisional[ Left Middle\60100145^ADF_399A W 12-0026BATTTTTTTCAAATATAGAAACTC CAAAGAAATTACATTATTATATCC F104G8E6FL27/03/96M27/03/96N28A45oC annealing @A>     >[ Left MiddleATTAAAATAATAATGTAATATAAAAATTATCAAATAAATCAATTAATCTTTTTCTTATTTTTTTCAAATATAGAAACTCAATAATGATCAAACGTTTATAATTTTTTTTTCTTTTTCAAATTAAATATATAATCGAGGGGATATAATAATGTAATTTTTTTATAATAAAATATAAAAA @A  I237 357J12L27/03/96M27/03/96N28P34R3D7 MunI3D7 MunIA45oC annealing @A>     >TAnonymous markerU3D73D7V177 bpWcompleteXYesYFinal[ Left Middle\100145^DK1. Homology with outer arm dynein light chain December 1995 Genbank protein acc. no. U194900001 2. Could not get a signal hybridizing Y1103 (Mun1 12D11) with pBR - deletion in vector sequence?L27/03/96M14/04/96N29P34126R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V192 bpWcompleteXNoYFinal[ Left Middle\100145225qb[ Middle Left\75110225^GDCA_1081212A W 12-0016BAGACATGCATATTCATTCTGTTGCTTTTTCCATGTTATTTATTGATAF79G2C10RAAATGCATCATTTCATAATATGTCTTCTATATATAAAAACAATGTAATCAAAAACCAAGGTACCTTTCCCTCAGGTAATGAAAAATCAACAGACATGCATATTCATTCTGTTGATAAAGGTAAATATATATCCCTTTATAAGGATATATCAATAAATAACATGGAAAAAAAAATTCCTATAAATAATACTAGTAGTAATATTAACTCTTTTBTAT @A I620 1092 239 76 492J12?y% AZ B` C]Sg% December!)-.:=>CPQSU[DeletionDetectedIDirectionuDirectionsfDivisionUDnaPDyneinEachuElegansCEndswlciparum)M[FoundFoundyac-./0FragmentFrom)*-./0fimtuGamma>GdnaGenbank)=CMPQSU[GeneQGetGlucoseUGroup-./0Grow)*GyrasePHas-./0IJKLMNOPQRSTaHaveHeavy:Homolgy:Homology!)-.=>CMPQSU[HybridizingInuQA W 12-0028BAAATTTAAATATTGAAGAAGAAA CAAATTTAAATATTGAAGAAGAAA F170G1B12F@A>    >TAnonymous markerU3D73D73D73D7V192 bpWcompleteXNoYFinal[ Left Middle\100145225^DFDE_991110A W 12-0028BAAATTTAAATATTGAAGAAGAAA CAAATTTAAATATTGAAGAAGAAA F170G1B12FL27/03/96M27/03/96N30A42oC annealing@   >LxGKEHomology with var-3 December 1995 Genbank protein acc. n.o.L406090001L21/03/96M27/03/96N1P414R3D7 MunI3D7 MunIA50oC annealing@A>    >TAnonymous markerU3D73D7V321bpWcompleteXYesYFinal[Left\7075^FB_12ATTATGAAATGAATTACTTGTATGAAAATTTAAATATTGAAGAAGAAAAGAGAAAGCGAAAATTAAGCAGCCTGAAAAATTATGAAAAAGATTTAAAACAAACCATTAATAAATTAAAGAAATATAAAATTATAAATGAAAATATTCAATATATTAATTTAGATGGTAATGGAAAAGGAGTGTATAAAATAATCGATGTGGATATAAACCAB AAAAGAAAAAG  @A  I538 3 619 724 822J12L27/03/96M27/03/96N30P61789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA42oC annealing@"[Ã( !"#$%Ã(` !"#$%-./0arsuvÃ)VWX`Ã)`13456Ã* Ã3`  !"#Ã4CDEFGHIJKLMNOPQRSijnÃH+,-.a1k4k5k+,-.789:;<=BCDEHISTUVXYZ[abcB $%'()*./01567VWfC  !"#$+-01345679:;<=>TUVWX^_`D !"#$()*+013456?@ACDEFGHIKLMN2SA@[DOFIJJ CGPRIMER BA AB E P ^ B DFHEC AV/ACEG @BBAAC B!A"A#A$ @%`&B'"E()ALK@x^At@IWM@TBUBWT @jJi Ah OA>    >TAnonymous markerU3D73D73D73D73D7V221 bpWcompleteXYesYFinal[ Left Middle\22575175175170^EADEE_103746A W 12-0029BATCAGTAAAAATTATTGACCTTT CATATTTTTAAATCAGTATGTGGT F185G7A2FL27/03/96M27/03/96N31A42oC annealing@A>    >TAnonymous markerAATGGTAAGAGATGATGGCTGGGTAGGTTTTGATAAAGCTGAAATTATAAAAGTATTTAATAATAATAGTAAAATTAAAGATGTACTTATACGTAACCTATCAAAAAATGATGAAATTATATCAGTAAAAATTATTGACCTTTATATGGATTATGTAAATAATCATAATCATGGTCATAATCATAATAATAATCATAACAATATTAATATABAATGATGATGACGAAAATAAAAATGATGAAAATAATAATAATGATGATGATATCTTAAAAAGAATTGCTGGACCACATACTGATTTAAAAATATTAAACCAGTTAGCTATAACATATATATGTAAATGGTTAAAATATAATATATC e@A I538 414 619 724 822J12L27/03/96M27/03/96N31P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA42oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V356 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_106746 A W 12-0030BTCTTATTATCCTTTTTATTTATG CTCTTTTTCTTCTTCCTCTATGAA F386G8E7F8 414 619 724J12L27/03/96M27/03/96N32P6578R3D7 MunI,- !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvw   @ Find New entryTestFind all/Control ScreenChromosome sort STS Sort Probe SortUpdate for Ross AFind_zTctttttcttcttcctctatTgajsvTgagagtgaagaagctttaa%TgatggacgtctaggaattaKTgcctctattgtgtatacatTgtrvTgtggtggtggttttggtagTgttacaacctattttgcctHTtaisTtaaataatttaaatgatgtNTtaattaagtgttctaaaacTtagttatacagacattcta_TtcgrtTttatatactcatttttagtTttcaataattacatttctgYTttgaatgtagactttttac#TtttcgtcggaggtatttgtPTtttgttcctaatttatcct(ÃIp Ã1b12f1d11r1e1f 1e9f1h112a7f2c10r2e4r2e7f 2f6f2g9r3a8r4b4f ACTTATTATATATTTGTAATATGTATTTCCAAAATTTGAAAGATGATATATATTTTTTTTCTTATTATCCTTTTTATTTATGGCCAGTTGAATTAATGAATTTTATTCATAACTTTTTATTATCAAATTTTAATTTTTTTTATAACGATATTATATCACTTTTTAAAAACTATAAAAAAGTTGGATCATATGAAAAGAAAATATTTTCATCBTAAATATTATCATAATATAAATAAAAAAGTAGACCTTTTTTTGAATAATTTTTATAGCTCACAAGTTGAAGACATGTCCCTAGAATCCTTTATGAAAGAAACTGATTTTTTTAATAATAAGAAACAAGAAGGTGAAGATAATAATTATAATAGTAATATTAATAATTATGATAATATCGAATGTATATCTCTTGATATTTATGGAAAAAACDTTTCATAGAGGAAGAAGAAAAAGAATGTTTACATAAAAATGAAATATCACAAAGAATATTATTAGAA  @A I538 414 619 724 822J12L27/03/96M27/03/96N32P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V487 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_106746!A W 12-003127/03/96o!I538 414 619 724 822J12KNHomology with arginyl tRNA synthase December 1995 Swiss Protein acc. no.P37880L27/03/96M3/10/96N33P65789R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA48oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V378 bpWcompleteXYesYFinal[ Left Middle\22570175175170^ECDEE_10674!BAAATCAAATATTGTTTCAAAATG CCTATGTTATCGACTGTTCTGGAT F247G7C4FAACTTCGTGTTTTAAATTTTTTATTATCTTCATTTAGAACGAAACCAAATCCTACATGCATTAATTTTGATTTTTATCTCCCCAGTTCGTTTTTTTAATCAAATCAAATATTGTTTCAAAATGTGTTAGTTGACCAATATCAGTAACATATATAACACAATTACAATTTAATTGTGTTAATCTATAATATAATGCAGCTACATCTGTAGAABTCATAACCATATCCCCCGTTCGATTTTTGTAAAAATAATGGTACTTTGAAATTTTCTGATTGATAACATATGGCATCTCCTATATTTGTTAATAATTTACTTTCCTTTAACAAATCCAGAACAGTCGATAACATAGGTACATAGAATGATTCACCAACATACTCTAAT {@A o"A W 12-0032BATTTTCTAAATTACCATTCTTTG CCCTTATTAGAGGATAGTGAACAA F128G5F12FAAATATCTTTATAACATTTTGTAAAGCTATCTACAATTGATGATAAGATTATAAAAATATTTTCTAAATTACCATTCTTTGGAATATCTTCACCTAAATATATACCTAATTCCTTAACATCTTTTTTAGTTTGTTCATATTGTTTTTGTGCTGTATTATATTTTTGTTCACTATCCTCTAATAAGGATACCATTTTTTCCTTAAA @A I538 414 619 724 822 705J12L27/03/96M27/03/96N34P657898R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIJ‚!‚" *-MNOPQRuv‚# -BCDESTUVWX]^_`bck ABHH +7G{HH%.d'@Dw/11/961/97ATTTC CTAGTTAGCC ATAGAATGGT TGATTAGGCA GCCTTTTCAA AAAAAAAAAA AAAAACAACA AAAAAAAACC ACCATAAAAT AAAATAAA"3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V204 bpWcompleteXYesYFinal[ Left Middle\2257017517517055^ECDEEC_1067469#A W 12-0033BGTATACTGGATCTAACTTCTTTT CTTTGAATGTAGACTTTTTACCAA F273G7D9FL27/03/96M27/03/96N35A44oC annealing@/Tttttccatgttatttattg1019c`1033ca11t2%2f6f3'k1cl12c45b60@Abra6Atpase%Clathrin$Cpn@CspiCtrpmK1cl12bOraPoltRnat$u‚J‚H‚X‚+‚R‚4‚K‚3[‚Ã!0Ã!PÃ!pMÃ!Ã"jnÃ"07Ã"@-Ã5 Ã7%Ã8@#ÃC0ÃGÃIp Ãd$Ã1b12f1c10r)1d11r1e1f 1e9f1h111h5r+2a7f2c10r2e4r2e7f 2e8r'2f6f2g9r3a8r3c10r(4b4f#AATAAAGTAAATCCTAAAGCAACTAAATCTTCATTATCATTTATACCATAATTTACATAATTTCCCCATTTTAAAACAGCTTTTAATAAAGATCTTAATCGTATACTGGATCTAACTTCTTTTACAGCTTTATCATATATATCTAAATCTTTATTTATTTCATTAATCATTTTATCATATTTTAAAGAAAATAAACATATTTGTATTTTACBTTTGACATTTATCTAGATATACAAATGGTATTAATTTCCTTTCAACATCTCTGAATTGTTCTGTTTTTATTTTTTCATTTTCTAATTTTGGTAATATACCGTCACATTCTTCTTTGGATGGAATAATATGTAATACTTTTTGGTAAAAAGTCTACATTCAAATCCGTCAGATCAAAAGAATTAATTTTATCAACAATTTCTTGAGTACTCCATTGGAAATCTTAATAAAATACCAATATTT @ #A I538 414 619 724 822 705J12L27/03/96M27/03/96N35P657898R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V450 bpWcompleteXYesYFinal[ Left Middle\2257017517517055^ECDEEC_10674#69$A W 12-0034BTGGTCTCTTCAAATTAGA CCTATAATATGGTACAGTTG DCLATHRINF530GCLATI619 724 822 705 594J12L27/03/96M27/03/96N36P78987R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesYFinal[ Left Middle\1751751705560^DEECB_7469$6%A W 12-0035BATAACAGAACCGTCAACAACT CTGAGAGTGAAGAAGCTTTAAT DATPase 2F260GATP2I724 822 594 495 1122J12L27/03/96M27/03/96N37P897612R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D73D73D7WcompleteXYesY Provisional[Middle\1751706080 130^EEBBF_466H[W  !"#$%'()*+,-./012356789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ\]^_`abcgijkmnqrstuwW12-0024W12-00494W12-0088[W3fW3-0015v&L27/03/96M27/03/96N38'A W 12-0036BAGGCCTCAACAAGGACCGAGTAT CAGGCCTCAACAAGGACCGAGTAT F154G2E8RACATGAAATCTCTTTCTTTATATTCAGGTGGTAATTGAAACCACGACTTATTATAAAATCCCATATTGTTATCATCATCATCTAACTCTTCATTCAAATGTAATGTTGAAATAATTTTATCATTATATAAAGTATTCTTTTCCTTTATAACATTATTTTTAATTGCTTCTTCATAAGCACATGCTTCTAACTTGGTTAATATATATTTATTB*TATTTTTTTATCATTATTATCATCATTATCAGTCTTTACTT @A I495 1122 1231 501 1125J12I495 1122 1231 501 1125J12  Aggcctcaacaaggaccgag'Agggggtggtttcaatcctt0AggtgtacacgtaactgctcIAgttagttgatttgatgttt(AtavAtaaaaaatgaacaagttaaKAtaaagacatgaaatcatgtwAtatcgtagaatgtttgcttAtatcttcagatactggaac[AtattacaaggtttaggaggAtattatgcttcaaagattc]AtattcatacaatttctgagAtcvAtcagtaaaaattattgaccAtgijtAtggctttctatactgtttcSAttfgsAttatatttaaagttttcagTAttattaatataatctttca AttgaaaaagcaaagagaaaAttttctaaattaccattct"AtttttttcaaatatagaaaCfkCagCaafst'K YAC 1125 is a multitransformant.L27/03/96M27/03/96N39P61213612R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA47oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V251 bpWcompleteXYesYFinal[Middle\8013055115^BFGBF_36799(A W 12-0037BAGTTAGTTGATTTGATGTTTTTA CTTTTGTTCCTAATTTATCCTTTG F142G3C10R@(AGCCACCATAAATTATTATCTCTAATTTCTTTGGGTAATTGTATACATCTTTCGGTATCTTCATTTAATGCTTCAAAATGAAAAGTTAGTTGATTTGATGTTTTTAACAATGGTACATATTTTTCTGGATATTCAACCATTAAGTGTACATCAAAAAAAATACTTGGGGTATATTTTTTTAAATTATTAATAACAGGTGGACCAAAGGATABAATTAGGAACAAAATGCATATCCATAACATCTAAATGGATCCATTCTGCACCCAAAGATTCCATTCGTTGTGTTTCTCCAGCTAATTGGCTTATATTGGATGCAAGTACTGAAGGGGAATTATAGCTTTTTAACGTACCCATCTCCAGCA  i@A  I495 1122 1294 1231J12L27/03/96M27/03/96N40P6121413z(R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43oC annealing @A>     >TAnonymous markerU3D73D73D73D7V360 bpWcompleteXYesYFinal[Middle\801308055^BFDG_36107)A W 12-0038BGTATTAAATTACTTTCGGGTTCC CAATGACAGGGTCAGATGAAGAAT F140G1C10R27/03/96M27/03/96N41A46oC annealing@A>    )ACTTTTTCTTAAATGTTGTCAAATGTATTAAATTACTTTCGGGTTCCCAAGTATTCTCATCATCTGAATATCCTTTCCATTTTACTAAATAAATAAAACCATTCTTCTTTTTTTTTATTTCAAGTATATCACCAATTTCAAATTCTTCATCTGACCCTGTCATTTTTTCTTCTTTATTATAAAACTACTTTTATTTTGTGTACAAGTAGGTBATATTGTGTATGTGTGTTCTTTC @A I1122 1294 1174 461J12 A1. Homology with P.falciparum cDNA clone Pf0131C December 1995 Genbank Nucleic acid acc. no. T02521 2. Y461 (Mun15G5) would never grow from our -70oC stock * A4Y461 (Mun15G5) would never grow from our -70oC stock4@A> ,  - >L27/03/96M14/04/96N42P121413514R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunIA46oC annealing@A>    >TAnonymous markerU3D73D73D73D73D7V356 bpWcompleteXYesY Provisional[Middle\130809045^FDBG5*G7D4RACGTGATAAAGAAGAAAAGTTACAAGAAAAATTAAGAATGAAACAAATAAAGAAGCTTTTAGATGAAAAACAAATGGTTATATGTAAAATTGAAGATATAGCTAAAAAGAGAAATAAAAAATATGTAAAAGCACAAGTATTATCTAAAGATTCTAAAATTGAACGAGCCTATTTTAGTCATAAAATGAAAAAATTTTATAATTCCAAATCTBAGCTATTTTGGTTGTGGATGGTCAAGTAACAAAAAGGAATGGACTCCATTTATTCATGCACCTTTCTTTGAAAAACAACATAACGCAATTTATAAAAATAGAAATAAAAAATTGTATGAAGAAATATATGATACATTATTACATGG e@A I1122 1294 1174 461 1295J12 >hCgtgataaagaagaaaagtt*CgtgtcttaamCgttgataatmCtaaaacttgmCtaatatatatCtaatccagtmCtacacataatCtaccctttttCtacgatatgmCtactattcamCtactttcattCtagtaaaaamCtagttagccmCtataaaagctCtatacctcctCtatgacatttCtatgcaaaamCtattattaatCtcaacaaatmCtccagttttmCtccatcaactCtccttaatccaatatttgaWCtctgaatacmCtgagagtagtCtgatttgatmCtgcaagtatmCtgcatgttcmCtgctgctgamCtggaaaattmCtgtggataattcttggtccECtgttatatgmCttacggatamCttagcagcamcgaf +A>    >TAnonymous markerU3D73D73D7B8V160 bpWcompleteXYesY Provisional\1303090370^FDCA_661067V356 bpWcompleteXYesY Provisional[Middle\130809045^FDBGD_61010511+A W 12-0040BTAAAGCTTATATTAAATGGTTGC CTATAATCATAGTGTGGGTAAAAT F83G1H5R22 1002 1186 582J12L27/03/96M27/03/96N43P1211137*D_61010511+A W 12-0040BTAAAGCTTATATTAAATGGTTGC CTATAATCATAGTGTGGGTAAAAT F83G1H5RAGGGTACCTTGTTGGATTTCTTTAATAAAGCTTATATTAAATGGTTGCGATTTTTAAACTTAAAAGAAAAGACGGATGAACTTATATTTTACCCACACTATGATTATAAATTAAATATACCTTCTGTTCTTAATTTTGTAAAATCAAAAAAAAAAAAAATT @A I1122 1002 1186 582J12L27/03/96M14/04/96N43P1211137R3D7 MunI3D7 MunI3D7 MunITony Triglia size selectedA41oC annealing@%,A W 12-0041BTTAAATCATGTTGATACAAAAAC CAAATATGTTACATCATCCACTTT F232G8B10FACAGCAATAATAAGATGTTAAAGGAACTGATTCCCCCCTTGGAGGCATCGTTTAAATCATGTTGATACAAAAACATTTTTAAGAATATAAGTATATATTTTATTAATGGATTTTTTTGTATATAGGTTTGTGTTTGTATTGTACTTGTGAGGGCACTTAGTTGATTAAAAGAAATATCAACCGATAATTTTGTATAATTAAAAAAGCATTTATGGATTTTTTTGTATATAGGTTTGTGTTTGTATTGTACTTGTGAGGGCACTTAGTTGATTAAAAGAAATATCAACCGATAATTTTGTATAATTAAAAAAGCATTTCBCCATTGGACTCTTGGTTCTATCC CAACGTTCGTTCATTATTTCATTT F234G6H2RATTTTAATGGATCTATAATTATAGCAAAGTCTCAAGATGGACCAAAAGAAAACAAGTTGATATATTTTCTTTCGATTTAATTCTTATACCCTTACACGTGGGTGGAAACCATTGGACTCTTGGTTCTATCCATATGAAAGACAAAAAAATATGTTTATATGATTCTTTGAATGGATCAAATAAAAAGTTTTTTGAATATATGAGAAGATATBATAGTTGATGAAATGAAGGATAAAAAACAAAAGGATTTGGACATATCCTTATGGACTTATAGTAAAGAGGGCGTTTCTGAGGTATCTTAATATATAAAAAGACTGAATAAATGAAATAATGAACGAACGTTTAATAGTATGATGTATATAAATATTATTATATATATGTATATGTATATATATATGTATATATGT @%36sF_99A W 12-0027BTGATTAGCACAATCAATAGCATC CAAAAACAGGAAAAAAGAGTTCTC F126 G5D9FATTCCTTTAATAAAAAAAACAGGAAAAAAGAGTTCTCAATTATAAAAAAATGGCTGATAGAAAATCTAATAAAAATGCAGTTGTAAAAAATGTAGATATGACAGAAGAAATGCAAATTGATGCTATTGATTGTGCTAATCAGGCCTTAGAAAAATATAACGTCGAAAAAGATATTGCTGCACATATAAAAAAG @A I237 357 1103 538J12{| A>    >TAnonymous markerU3D73D7V200 bpWcompleteXYesYFinal[ Left Middle\130225^GA_412d‚U,‚V-‚W.‚X/‚Y0‚Z1‚a2‚b3‚c4‚d5‚e6‚f7‚g8‚h9‚i:‚j;‚q<‚r=‚s>‚t?‚u@‚vA‚wB‚xC‚yD‚zE‚F‚G‚H‚I‚J‚K‚L‚M‚N‚O‚P‚Q‚R‚S‚T‚U‚V‚W‚X‚Y‚Z‚[‚\‚]‚^‚_‚`‚a‚b‚cÃ!dÃ! eÃ!0fÃ!@gÃ!PhÃ!`iÃ!pjÃ!kÃ!lÃ!mÃ"nÃ" oÃ"0pÃ"@qÃ"PrÃ"`sÃ"ptÃ"uÃ"vÃ"wN% ‚#Ã#`a 0424cM1921125'1212c1214-./018501995!)-.1f9-./02)-./03.03d70548./01262069-./06f8./01270oc)*81209d80A'-./012Acc!)-.Acid)AdditionalAnalysisAndArginyl!ArmB8Be-./0BeenBelieveButByCamp-dependent-Cdna)Chain-Chimeric-./0Chromosome-V-BAGAGCTAATTCTCCAAAGTAATC CGGAGAAGAAGGAGATACATTTTT F113G5H12FATTTTATAATAGCAGCTCTTGGTTTATTTTTAAGAAGAGCTAATTCTCCAAAGTAATCTCCCTTTGTATAAGTTTTGATGACTTTATTATCTTTAGAAGCTACAGGATTTCCATCTATAAGGATAAAAAATGTATCTCCTTCTTCTCCTTCTTTAATAATAATTTCTCCATCATTATAACTTTT @A I582 36 243 1211 69J12   >TAnonymous markerUB8V369 bpWcompleteXYesYFinal[ Left Middle^A_6-A W 12-0042BAGAGCTAATTCTCCAAAGTAATC p   )*+,-.3456>?@ABCDEFGHIJKLMNOPQRST02WXYZ[\]^_`abc:dC!789:;<=UVe_hmltop1fgijkmnqrstuvvvvv0@P`p  ‚! ‚" ‚$ ‚%‚&‚(‚)‚*‚1‚2‚3‚4‚5‚6‚7‚8‚9‚:‚A‚B‚C ‚D!‚E"‚F#‚G$‚H%‚I&‚J'‚Q(‚R)‚S*‚T+ jÃ"@Ã"P:Ã"` Ã"ZÃ#qÃ#`0Ã#pÃ#"Ã$ Ã$@UÃ$p\Ã%)Ã%0(9Ã%`TÃ%p/Ã% WÃ% Ã&gkÃ&P'Ã&pSÃ&OÃ&LÃ&QÃ'vÃ'@DÃ'p;Ã(Ã( BÃ(PPÃ(pÃ)`Ã)pÃ)1Ã*rÃ*@?Ã*P]Ã*pÃ1wÃ1P2Ã2aÃ2*Ã3`Ã3pEÃ3Ã3Ã4 Ã4 FÃ40,Ã4PC1b12f1c10r)1d11r1e1f 1e9f1h111h5r+2a7f2c10r2c3f72e4r2e7f 2e8r'2f2f.2f6f2g9r3a8r - >TAnonymous markerUB83D73D73D73D7V183 bpWcompleteXYesYFinal[ Left Middle\3708550100175^ACEEF_6123119.A W 12-0043BGTTATGAGAGATGAAAAAGAACA CAATTGTGGTGATTGAGGTGATGC F257G2F2FAGATGATATTATGTTAAGAGAATTATTTGAACCATTCGGTACTATAACATCAGCAAAGGTTATGAGAGATGAAAAAGAACAAAGTAAAGGCTTTGGTTTTGTTTGTTTTGCATCTCAAGAAGAAGCAAATAAAGCAGTAACAGAAATGCATTTAAAAATAATTAATGGAAAGCCATTATATGTAGGATTAGCAGAAAAAAGAGAACAAAGA- A1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. Sequence homology with cAMP-dependent protein kinase regulatory chaB.in December 1995 Swiss Protein acc. no. P39077 L31/03/96M14/04/96N45P713131RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunI3D7 MunIA45oC annealing @A>    .BTTATCAAGATTACAACAAAGATTTCGTTTGCATCCAATTAGACATCATATGAATAATCCATTAAATACCCCAATGCAATATGCATCACCTCAATCACCACAATTACAATTTAGTCAGAATACATTAAGTTATGG Y@A I582 69 790 548J12 A1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. YAC 548 (Mun1 6F8) is a multitransformant 3. Sequence homology witAGCCATTATATGTAGGATTAGCAGAAAAAAGAGAACAAAGAH12-0043.12-0044/12-0045012-0046112-0047212-0048312-0050512-0051612-0052712-0053812-0054912-0056;12-0057<12-0058=12-0059>12-0060?12-0061@12-0062A12-0063B12-0064C12-0065D12-0066E12-0067F12-0068G12-0069H12-0070I12-0071J12-0072K12-0073L12-0074M12-0075N12-0076O12-0077P12-0078Q12-0079R12-0080S12-0081T12-0082U12-0083V12-0084W12-0085X12-0086Y12-0087Z12-0089\12-0090]. BKh polyadenylate-binding protein December 1995 Swiss protein acc. no. P31209L31/03/96M14/04/96N46P7196RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunIA47 oC annealing@A>    >TAnonymous markerUB83D73D73D7V344 bpWcompleteXYesYFinal[ Left Middle\370175125^AFBF_69108I3D7 MunI3D7 MunIA47 oC annealing/K1. The Oxford group has foundYAC 69 (Mun1 1F9) to be from chromosome14 containing three chromosome 14 STSs; likely to be a chimeric YAC. 2. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N47P1916R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA43o C annealing@A>    >TAnonymous markerU3D73D73D73D7V240 bpWcompleteXYesYFinal[Middle\175125115^FBH Chromosome./0Chromosome14-./0ChromosomesIJKLMNOPQRSTaClone)MComplementConfusionContaining-./0ControlCouldDatabaseimtDecember)/A W 12-0044BTTCCTCATCCTTAGTATCATAAT ecember 1995 Swiss protein acc. no. P31209L31/03/96M31/03/96N46P7196RTony Triglia size selected3D7 MunI3D7 MunI3D7 MunIA47 oC annealing@A>    >TAnonymous markerUB83D73D73D7V344 bpWcompleteXYesYFinal[ Left Middle175125^AFBF_69108/A W 12-0044BTTCCTCATCCTTAGTATCATAAT l/CGTAGTAATAAAACGAAAAGTTGCF146 G6B9RAAATTAATTATATTAATCTAAAACAATCAATTATAAATTAAAATAAAATGATAAAACCAAAATAATAATAAATAAATATGGTTTCATTCCATATTACCAAATTATTATATTATATTATATTATATTATATTTTGAATTGATTAAAATGAATAAATAAATAAATATCATATATATATATATATATATATGTATGAGAAAGATATAAATAAATBATCATACTGATATATATATTTTATATGTAT @A I69 790 91 548J12O/F_910780A W 12-0045BAGGGGGTGGTTTCAATCCTTTTT CATGACCACCCCCTTTTTATCAAT F125G8H4FAAACATTTAATATTTTTTTATGTTGTGAGGGTATTGATCAAATTAGGGGGTGGTTTCAATCCTTTTTTTTTGTTTTTTTTTGTTTAAATTGGTTAAACCAAAGGAAAAAAAAAAGGTCCCAATTATTGAAAAATACTGATAAAATAATTGATAAAAAGGGGGTGGTCATTTTAAAAAGGGAAAAAATAAAAATTAATTTAAAAAATTATACB/CTTAATTAATAATAATAATAATGATAATAATAATAATAATAATAATAI1266 69 790 91 548 603 788 1196J12167913R3D7 MunI3D7 MunI3D7 MunID7fCatgatgactgctatctaccCattcatattcattctgttgCcajCcatttgtttgtactacgtt`Ccttattagaggatagtgaa"CcttctcattcagttcaaaaDCgafsCggtataattaataaatgaa CgttaaggaatatactcataWCgttccatgtcttctcatct>CtagvCtaaaggaatagaggactgg4Ctataatatggtacagttg$Ctatgttatcgactgttctg!CttgstuCttatcacattattggttgaCttttcaaagttttctgtgc;GuGactgtgtctttttcatctgGatfGatccaatataccatcagcaGatcttaagatataaacattGattggtgtgatggttgatgGctfikGgafGgagaagaaggagatacatt-" Middle0123456789:;<=>?@ABCDEFGHIJKLMNOPQRabcjkmnqrstRightBCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`bctuvw‚A+‚FVW‚Q0u‚V*MNOP‚a-?@‚f"#$'(j‚q $%34567‚v345‚ !"#01567k‚[]^_`‚%'()*j‚-01\]^fk‚)*+?@A‚?@mqÃ!-BCDEMIJKLÃ&789:;<=Ã&`KLMÃ'`013d110vw11+TUu12%'()*+M_mquv13'()*+-013STUV]^_jn14 ()*0_u15?@AKLMSTfgi234567JKLMNOPQRS]^fgjknst127 !"#$%+,-.018 !"#$%9 !"#$%./013d7  !"#$%'()*+-./012Muni  !"#$%'()*+-./012N  ÃÃ`?@ÃÃ013456Ã0 !"#$%Ã0vwÃpÃ@uÃpTUÄ!+Ä!wuÄ!Ä!uvÄ!Ä"Ä"mqÄ"'_Ä"(MÄ"3%'()*+Ä"6'Ä"qSTUVÄ")*Ä"+Ä"_Ä"013Ä#"-Ä#5]^Ä#B'(Ä#SjnÄ#fPP1A W 12-0046BTCTAAATGACTCTCTTCAGAAAT CACAAATAAAACAAATAAAACTTA F187G6B2Fng@A>    >TAnonymous markerU3D73D73D73D73D73D73D73D7WcompleteXYesY Provisional[Middle\401751251158570165^BFBHFCBD_6910783881A W 12-0046BTCTAAATGACTCTCTTCAGAAAT CACAAATAAAACAAATAAAACTTA F187G6B2F 548J12L31/03/96t1A43oC annealing@A>    >TAnonymous markerU3D73D73D73D73D73D7V218 bpWcompleteXYesYFinal[Middle\1158570165185^HFCBDD_7838882A W 12-0047BAAACTAACAACGAAAATGCTGTC CATTAATAGATATGTCCTGTGTGG F204G3F6F8J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N50P6R3D7 MunIA45oC annealing@v  Mun1Mun15g5)*Myosin:NNever)*No!)-.:=>CIMPQSU[NotNucleic)MNucleolar=OOfOkOnIOne0uOrOrderedIfuwOrf[OriginalOur)*OuterOverlapIwOxford-./0P)M[P31209.P37880!:P39077->PbrPcrsPf0131c)Polyadenylate-bindin.PositivePrimersIfuwProliferation-associ=Protein!-.:=>CPQSU[RIReactionsuRegulatory-Restriction1ACTTAGTATTATCTAAATGACTCTCTTCAGAAATTACTTTTAACTGTATTTTTTTTTCCTTTTTATTATAATGTACATCACTACTTTTTTCATGTTCATAAGATGAAGTACTATGATAATAACCATAGAATAATTTGAATAATTTTCACGGTGTTTTTTCGTTTTTATAGAAAAATAAGTTTTATTTGTTTTATTTGTTTTATAATTTTTAB TTTTTTTT @A I91 548 603 788 1196 812J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M31/03/96N49P1679139R3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI3D7 MunI*r3A W 12-0048BTATTTTCTATTTTTTATGTGTCC CAATTTCAACAATCTTATTCATCC F96G5C11FAvATTATTATCATCATATATGTTATTTTCTATTTTTTATGTGTCCTTTTTTATGTTTACACTATCTTGATTAATATCTCTAATGTCCGTTGTGCACGTGGATGAATAAGATTGTTGAAA v@A I1196 812 475 121J12L17/04/96M17/04/96N51P13952R3D7 MunI3D7 MunI3D7 MunI3D7 MunI196 812 475 121J12L17/04/96M17/04/96N51P13952R3D7 MunI3D7 MunI3D7 MunI3D7 MunI,BAATAATGGGTACTTTTGATGTACAATTTTTTTAATACTAACATTTTCAAAAAGTGGATGATGTAACATATTTTTATATAATTTTCTAATATTAATTAAATCTGATTGTATTGTATTATATATACATATATCAATATCTGAGTTATATATATCTATATCA r@A I582J12L31/03/96M14/04/96N44P7RTony Triglia size selectedA42oC annealing@A>    >TAnonymous markerUB8V369 bpWcompleteXYesYFinal[ Left Middle\370^A_6-A W 12-0042Wj{TAnonymous markerU3D73D7B8V256 bpWcompleteXYesYFinal[Left\190135120^ECH_5111A_412 ) @A>  >L27/03/96M14/04/96N41P1214135R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA46oC annealing@A>    >TAnonymous markerU3D73D73D73D7V233 bpWcompleteXYesY Provisional[Middle\1308090^FDBG_610105*A W 12-0039BTTTAGATGAAAAACAAATGGTTA CAGGTGCATGAATAAATGGAGTCC F217f9 ) @*G A+A,@B-B A.B B/@CK0KOPA2A3A4@AA5BT6\7@8B[9B[:K;AT<A=AK>AK?AA@[BACBADFEFA T GB[HAIBAAJJKKLB 1)-./012>IKLMNOPQRSTUV[m10921125'12IJKLMNOPQRSTa12c1212d1113IJKLMOPQRST13a8STUV14-./0a15d6KLM18501995!)-.:=>CPQSU[9d80A'-./012Acc!)-.Acid)AdditionalAnalysisAndArginyl!ArmB8Be-./0BeenBelieveButByCamp-dependent-Cdna)Chain-Chimeric-./0Chromosome-+^DFDE_991110*2AAGGACCTCGTGCTCTTCAGGTTGCTCGGTGTGTAAGTACCAAAACTAACAACGAAAATGCTGTCGCAAATATTATATACAGAGTTTTATCTGGAAGGCGCTCTTAATTAAAAAAAATATATATGTATAAAACAAGCAAAATTGTATTATATATACACAAATATTTATGTGTGTGTTTAAAAAATATATATATATATAATCACAATGTCCCBZAATAAAACAACCCCACACAGGACATATCTATTAATTTTATATAATATGTATATATATATATATATATATATATATATGTGTGTATATGTG,I548J12K,1. YAC 548 (Mun1 6F8) is a multitransformantL31/03/96M07/05/96N50P6R3D7 MunIA45oC annealing@_)23-?@HIjmrs 56789:;<=IJKLSTUVW\]^km5 !"#)*345IJKMNOPQRVWXYZ`j6  !"#%'(./012TUVgij7 !"#$%+,-.01BCDEFGHIXYZ[`abc8 !"#$%9 !"#$%./013456?@3d7  !"#$%'()*+-./0123456789:;<=?@AN4AW12-0049BGGTCATGGTGATGATAACAATGT CCTAAAGGAATAGAGGACTGGTAT F91G8G2R >TAnonymous markerU3D73D73D73D7V117bpWcompleteXYesYFinal[Middle\1651856560^DDHC_88714AW12-0049BGGTCATGGTGATGATAACAATGT CCTAAAGGAATAGAGGACTGGTAT F91G8G2RL17/04/96M17/04/96N52A45oC annealing@A>    >TAnonymous marker[Middle4AAAAATACGACCACACAAATGATACAACTATTAATAATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATAATAATAATAAATATAATGATGGTCATGGTGATGATAACAATGTTGATAAGGGTACACACAAAAACAAACCAATBEACAATTACAAAATAAATACCAGTCCTCTATTCCTTTAGATAATGAAAGAAAAGAAAAAGAAAAAAAAA  @A  I812 475 121J12L17/04/96M17/04/96N52P952R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@5BTGCTCTTATACAATCTTTTAAAGTACACTGTCTATTAACTTTTTTTAAGATAACATCATCTATATGTTGAATACCTAATTGAACTCTTGTACAACCATAATACCTTAATCTTAGTAATTCATCTTTATTAATTTGATCAGGTCTTGTTTCTAAAGTTAAACCTATAATTCTGCAATTACTTTTTTCATTAATTTCTTGTTCTTCT @A I812 475 121 305J12L17/04/96M17/04/96N53P9524R3D7 MunI3D7 MunI3D7 MunI3D7 MunIA44oC annealing@A>    > 4A>    >TAnonymous markerU3D73D73D7V278bpWcompleteXYesYFinal[Middle\1856560^DHC_8715A W 12-0050BCAGCTTGTAAATCTGTTGAACTT CAAGATTAGGGTATTATGGTTGTA F239G7A11FAATATGGTTTATATTCATGATTATTATACCATTTTTCAATTTTAGTAAATGGTGTAATTTCACAAGGATAAATTTTCCATTGATCAGCTTGTAAATCTGTTGAACTTAAAACATATTTAAACATATGAATATCTTTATATACATCAGAATATGGTAGATCAGGCATTAGATGAATATCAACTTTAAATCCATTTTCTTTTAATAAATAAAT3A45oC annealing@A>    >TAnonymous markerU3D73D73D73D7V117bpWcompleteXYesYFinal[Middle\1651856560^DDHC_8871(:Ggtcatggtgatgataacaa4GgttggaaagagaagttcagQGtuaaattactttcgggt)GtgtgctattatgactcttaGttatgagagatgaaaaaga.Taaagcttatattaaatggt+Taagtatttgttttattatt Taatgattctaatataaaga9Tagatgtgatggtgtatgtg Tattttctattttttatgtg3TcatataagatgtgtaggagTccattattactttcttgtg7Tctaaatgactctcttcaga1Tcttattatcctttttattt TcttccttaagtgatgaaaaTgattagcacaatcaatagcTgcctctattgtgtatacatTggagaaaagaaattaggaaTggtctcttcaaattaga$Ttaaatcatgttgatacaaa,kAagattagggtattatggtt5AatjuvAataaattatctaacaaaca[Aatacatatgatgatacatt]AatataaatacgaaacattgEAatgacagggtcagatgaag)Aattgtggtgattgaggtga.Aatttatattattctcattg\Aatttcaacaatcttattca3AattttagatgcttatccaaZAckAcauAcaaataaaacaaataaaac1AcataattattttaaccaagAccsAccaattatattcatttttcActtgcttttataactatcaAgaikvAgaatatggatagacaagtt AgcagcaaacattaatgaccAggcctcaacaaggaccgag'Aggtgcatgaataaatggag*5TAnonymous markerU3D73D73D73D7V415 bpWcompleteXYesYFinal[Middle\185656070^DHCB_87156A W 12-0051BACCAGAACCTACTGTTACTAATGA CTCTTCCTCCGTTTCTTGATTAAGA DABRAF730GABRAI812 121 305J12L17/04/96M17/04/96N54P924R3D7 MunI3D7 MunI3D7 MunIA45oC annealing@A>    >TKnown gene sequenceU3D73D73D7WCOMPLETEXYesYFinal[Middle 6\1856070^DCB_8157A W 12-0052BTCCATTATTACTTTCTTGTGTTA CGTAGGTAGCTTTTTGATTCATTA F112G2C3FAAAAAAAGAATATTGAAAGTATACAAAATAAGAAACACTTAATTTATAAAAACAAATCATATAATCCATTATTACTTTCTTGTGTTAAAAAAATGAATATGTTAAAAGAAAATGTTGACTATATTCAAAAAAATCAAAACTTATTTAAAGAATTAATGAATCAAAAAGCTACCTACTCTTTTGTTAATACCAAAAAAAAA @A I121 305 293J12L17/04/96M3/10/96N55P244R3D7 MunI3D7 MunI3D7 MunIA42oC annealing 8BCACTTGCCAAAGATATCAATATTTGTTTTTTATCATCAACATAACATTTTAATACTTGACTTTTATGACCTATAAATGTTGTAAAATCTTTTAAATTCAAAAACATTTCATTTGTAACAACGTTTTGTGATTTAATAATTTTATTTGTTTCTAAATGTAGATAATTAATATTAAGATGATGACTATCATTAAAATCCTCATCACCCGGGACTTATTATTATCACCACTTGATTGG  @A  I293J12L17/04/96M3/10/96N56P4R3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D7V444 bpWcompleteXYesYFinal8[Middle\150^A_5CAATATTTGTTTTTTATCATCAACATAACATTTTAATACTTGACTTTTATGACCTATAAATGTTGTAAAATCTTTTAAATTCAAAAACATTTCATTTGTAACAACGTTTTGTGATTTAATAATTTTATTTGTTTCTAAATGTAGATAATTAATATTAAGATGATGACTATCATTAAAATCCTCATCACCCGGGACTTATTATTATCACCACTTGATTGG  @A  I293J12L17/04/96M17/04/96N56P3R3D7 MunIA43oC annealing@A>    >TAnonymous markerU3D7V444 bpWcompleteXYesYFinal$9A W 12-0054BTAATGATTCTAATATAAAGAGAG CTATTATTATGAATAGACATGTCG F142G6A9FAATGTGTGTAATGATTCTAATATAAAGAGAGATTATTTTAATTTGAATTTTTATCTTGATTATTATAATAAAAAGAAGAATGAATTTTTAAATGTGGCTATAAATCATGATGAAGATAAGGAGGTGAACGACATGTCTATTCATAATAATAATAATAATATCTATAATGATAATATCCATATGGATAATAATAATAATAATAATGATATCCTAATAATAATAATAATGATATCCF8m9BATAATTATAATATCCATAATAATGATATCCATAATAATAATGATATATATAATAATAATATTCATAATAATAATATCCAAAATAATAATATCCAAATAATAATATCCATATTAATAACCATTATAATTATAACTATAATCATTCTAGTGATAACATTATAAATGAAAAGTGTACTCATCATGCGACAGTAC @A I293 25J12L17/04/96M3/10/96N57P41R3D7 MunITony Triglia size selectedA45oC annealing@A>    >TAnonymous markerU3D7B8V402 bpWcompleteXYesYFinalm:KOHomolgy with Myosin heavy chain 95F Swiss protein acc. no. P37880 December 1995L17/04/96M3/10/96N58P41R3D7 MunITony Triglia size selectedA43oC annealing@A>    >TAnonymous markerU3D7B8V254 bpWcompleteXYesYFinal[Middle\150125^AC_51;A W 12-0056BAAAATTAAATAACCGTGGACTAT CCTTTTCAAAGTTTTCTGTGCTAT F166G3E3R25J1212-000112-000212-000312-000412-000512-000612-000712-0008 12-0009 12-0010 12-0011 12-0012 12-001312-001412-001512-001612-001712-001812-001912-002012-002112-002212-002312-002512-002612-002712-002812-002912-0030 12-0031!12-0032"12-0033#12-0034$12-0035%12-0036'12-0037(12-0038)12-0039*12-0040+12-0041,12-0042-_Tatactttccatgcttctct:TatctatattttccccctccTTatgaaggtggtttccatttMTattattatgaatagacatg9Tattttccacgatttcttta?TcagTcaaataatctatactaccg TcaaatgcatctttattttcVTcaataaaaggattagaaaaUTcattattattcgttgaaggTccf  19951f9-./0a2)-./0IKLMPQSTUm234120003C2h1JKLMNOPQRST3.0MS3d703g2I548./0126JKLMNOPQRST62069-./06f8./0127S70oc)*812095f:9d80A'-./012STUVmAcc!)-.:=>CMPQSU[Acid)MAdditionalAfg3QAnalysisAndIJelieveButByCCCamp-dependent-Cdna)CChain-:Chimeric-./0Chromosome-;AATTTTTTAATTATGCTGTTATGGATATCAGTATAAAAGAGGAACAAAAGAATAATGATTTCCTTACAAAATTAAATAACCGTGGACTATTAAAAGAAATGTGGGGATATTCATCTTTCCCACAGGGTATCTCATTATTATATTCAAATCGATTATCTACATATCCTCATTGGAGAATATATACATGTACGGATCTTATGCAATTAAGTAAB'TAGCACAGAAAACTTTGAAAAGGGAAAAGAGAAAAGAGA @A  I293 25J12L17/04/96M3/10/96N59P41R3D7 MunITony Triglia size selectedA46oC annealing@A>    >;TAnonymous markerU3D7B8V249 bpWcompleteXYesYFinal[Middle\150125^AC_51 <A  I293 25J12L17/04/96M3/10/96N60P41R3D7 MunITony Triglia size selectedA48oC annealing@A>    >TAnonymous markerU3D7B8V284 bpWcompleteXYesYFinal[Middle\150125^AC_51=A W 12-0058BAATATCAGTGGGAAAATGTGTTA CATTCAAGCCCTTCGTAATCTTAT F300G3A9FCGATCAAACACTAAAAAATATAAAAATTAATAAAATAATTATTTCTATATATTTCA @'&O11PA W 12-0077BAGAAGATGACGGAAGAGGTATCC CTTTTCGTCGGAGGTATTTGTATT F174G4F6RAATATTATTTGAAATTATTGATAATTCAGTAGATGAATATAATAATTTTGAATGTAATACAACTAGAGTCGTTATACATCAAGATGATAGTGTTACTATAGAAGATGACGGAAGAGGTATCCCTTGTGATGTTCATGAGGAACCCACAAAATCTGCCTTAGAAACAGTTCTTCCTGTTCTGCATTCAGGAGCGACATTTTTTGATGACGACBmATGGATGATGATATTGATAATAATATGAACAATACAGGTAATACAAATACCTCCGACGAAAACAATCGTGATAATATATCTAATGATAATAATAATAATAATAATAAAA?@A I181 479 40J1262 bpWcompletete< YSizeTony +,-.9:;<=>CDEFGHIVWXYZ[`amrsTriglia +,-.9:;<=>CDEFGHIVWXYZ[`amrs41oc +W42oc ,7LR]43o/43oc "(18:?N[\445ocv44oc#5DMOT^450cf45oc $%-23469>ABHJKSUV50ocIPAnnealing  !"#$%'()*+,-./0123456789:;<=>?@ABCDRB8<=>CDEFGHIVWXYZ[`amrsÃ! JÃ"3Ã%Ã%ZÃ&Ã'+Ã' kÃ'jÃ(Ã(BÃ)@-Ã)Ã)Ã*TÃ*0Ã*pUÃ*7Ã1Ã1 Ã1@RÃ1P"LÃ2@HÃ2PDWÃ21Ã3 Ã4@)Ã5/Ã5P Ã5;qÃ6 'Ã6P:Ã6pÃ60Ã70 FOÃ7Ã8@vÃ84Ã90 ]Ã9P<Ã9pSÃ:PEÃ:pÃ:aÃA2ÃArÃB0QÃB`\ÃBÃBPAgtttttataattggattga8AtaaaaaatatAtaaaaatgatAtaaaatacamAtaaaatgagtAtaaacgaaamAtaaagagaamAtaaagatggtAtaaagtaaatcctaaagca#AtaaatagtatAtaaatctaatAtaacagtagmAtaagataatmAtaagcaaaamAtaataaaaamAtaataataaimAtaatgctaamAtaatggtcgiAtacaaaacamAtacaaaatamAtacagatacmAtacatatacmAtacattattmAtacgagtaamAtacgcaatamAtagaaaagamAtagaatggtmAtagacgaatmAtagctgttgmAtaggacttgmAtaggttatgmAtagtacatamAtagtagaagmAtataaaagtmzAgtttttataattggattga@Ataaatgtaa@Ataatgggaa@Atataagactttaataagta@|Atatattggt@Atattagtga@AtcaccaaaatAtgacgaaacatcaaacttt@}Atgcaaaccc@Atgtggtaca@tatgt@eAttttttaattatgctgtta@~Cagcaataataagatgttaa@Ccaaatgcaa@lCgtgataaagaagaaaagtt@Cttagtattatctaaatgac@Gaaaacggat@kGaagaaaatc@Gaattcctaagaaacaaata@Gaatttcttccactttttgt@TGatatatttcacatgttaaa@Gatgatattatgttaagaga@jGccaccataaattattatct@iGgagaagaat=AATATAAGACTTTAATAAGTAAAGGAATATCAGTGGGAAAATGTGTTAATTCACCATATGGATTATTATTAACCAAAAATCAAATATTAAAAAATATTCATGAATATAAAAAAGGTTACTTCGAAATTCAAGATGAAGCTAGCCAAATAGTGAGTTCCAAAATACCTGTACATCCAGGTGATAAGGTGCTGGATTATTGTGCAGGGTCAGGBTGGAAAAACATTAGCATTTTCTATGTTAATGGAAAATACAGGAAAAATATATTTACACGATATACGTGATCAAATGTTATCTCAAGCCAAAATAAGATTACGAAGGGCTGGAATACCAAATTATATTQ@A I293 25J12L17/04/96M17/04/96N61S48oC annealingTAnonymous marker[MiddleXB3a9f=3c10r(3c12A3c3f<3e3r;3f1fT3f6f24b4f'=KjHomology with Proliferation-associated nucleolar antigen Genbank protein acc. no. X555040001 December 1995L17/04/96M3/10/96N61P41R3D7 MunITony Triglia size selectedS48oC annealingTAnonymous markerU3D7B8V337 bpWcompleteXYesYFinal[Middle\150125^AC_51GTTATCTCAAGCCAAAATAAGATTACGAAGGGCTGGAATACCAAATTATATTQ@A I293 25J12P>A W 12-0059BAAGCGACTCGTTGTGTAAAAATG CCGTTCCATGTCTTCTCATCTAAT F267G6A4Fcc. no. X555040001 December 1995L17/04/96M17/04/96N61P31R3D7 MunITony Triglia size selectedS48oC annealingTAnonymous markerU3D7B8V337 bpWcompleteXYesYFinal[Middle\150125^DC_111>A W 12-0059BAAGCGACTCGTTGTGTAAAAATG CCGTTCCATGTCTTCTCATCTAAT F267G6A4FL22/04/96M22/04/96N62A45oC annealing@A>    X6a9f96b11fQ6b1f6b2f16b5fO6b9r/6c12fZ6d11f 6h2rC6h3f 7a11f57a12f7a2f7a4f87a6fL7c4f!7d4r*7d8r7d9f#7e12r:8a11r8a12f[8a9r\8b10f,8d5f]8d7f8e11fU8e6f8e7f 8f5fM8f8fD8g2r48g3f8g7rN8h4f0Abra6Atp2%Atp4Clat$Cpn@CspiCtrpmJtkrJt108vJt33gJt58jJt90OraPfuPf3.3fPoltRnatY130ls1imt10imt1021imt1081imt1141imtaaga>AAGATAGTTAGTAAGCTAGCTTTAGAAGCGACTCGTTGTGTAAAAATGGATAATTTAATGGGAAGAAAAGAAATTGATATAAAGAGATATGCAAAGGTTGAAAAAATTCCTGGAGGAGATATTACGGATAGTTATGTTCTAAAAGGTGTTATGATAAATAAGGACATTACCCATCCTAAGATGAGACGTTATATTAAGAATCCTCGAATATBTATTATTAGATTGTACTTTAGAATATAAGAAAGCAGAAAGTCAAACTAATGTAGAGATATTAGATGAGAAGACATGGAACGAATTATTATTACAAGAAGAAATAGAAGTAAAAAAAATGTGTGAATATATAATAGATAGT^@A I25J12>K\Homology with T-complex protein 1 gamma subunit Swiss protein acc. no. P39077 December 1995.L22/04/96M22/04/96N62P1RTony Triglia size selectedA45oC annealing@A>    >TAnonymous markerUB8V350 bpWcompleteXYesYFinal[Middle\125^C_1?A W 12-0060BGCTACCTCTTTATTTCCATACAT CTATTTTCCACGATTTCTTTATTA F193G6A2FL22/04/96M22/04/96N63A43oC annealing@?AAGAAATACTTATAAGAGATACAAACGAAACAACTTCAATAAAAAATAATTTCTATTTTTCTCATGATTATTTATATATTCCTCAAGCTACCTCTTTATTTCCATACATATATGATTTATTAAAAAATAATAACAAACAGAATATACAAAAATGTAATGATACAATATATGTAAGAAATAATCCGTATAGTGAAAATACACCTAATAATAAByTATTGATGTAAATAATAATATAGAAAAAAAGCAAGATAATTATATTAATAAAGAAATCGTGGAAAATAATAATGTGCATATAATAAATAATTCAAACATATTAAATAAGCAACGAGCATC  K@A  I274 805 1383J12L22/04/96M22/04/96N63P3915R3D7 MunI3D7 MunI3D7 MunI?A43oC annealing@A>    >TAnonymous markerU3D73D73D7V330 bpWcompleteXYesYFinal[Middle\509590^GDD_1013@A W 12-0061BAGATAAGGTTTTTAGATTCTTCT CTAATTGTATCCGTCATTCATAGA DCPN 60F246GCPN 60I274 805 1383J12L22/04/96M22/04/96N64P3915R3D7 MunI3D7 MunI3D7 MunIS46oC annealingTKnown gene sequenceU3D73D73D7WcompleteXYesY Provisional1:`Taattgtatccgtcattcat@TactTacaatttttaattctccttTatkstuTataatcatagtgtgggtaa+@[Middle\509590^GDD_1013AA W 12-0062BGCTCTATCTTTAATAATCATATGTAAC CAACCATTTTAGATTCATCTTC F885G3C12I1383J12L22/04/96M22/04/96N65P15R3D7 MunIA45oC annealing@A>