Paper : "Biochem J_1997 Dec 15_328(3)_905-11"
Author "Gerold P"
Author "Vivas L"
Author "Ogun SA"
Author "Azzouz N"
Author "Brown KN"
Author "Holder AA"
Author "Schwarz RT"
Title "Glycosylphosphatidylinositols of Plasmodium chabaudi chabaudi: a basis for the study of malarial glycolipid toxins in a rodent model"
Journal "Biochem J"
Year 199715
Volume 328(3)
Page 905-11
Abstract "Biochem J_1997 Dec 15_328(3)_905-11"

LongText "Biochem J_1997 Dec 15_328(3)_905-11"
Free and protein-bound glycosylphosphatidylinositols (GPIs) of the
blood stages of the rodent malarial parasite Plasmodium chabaudi
chabaudi AS were identified and characterized. TLC analysis of material
extracted by organic solvents from metabolically labelled parasites
revealed a distinct set of glycolipids. These glycolipids were
identified as GPIs by specific chemical and enzymic treatments and by
structural analysis of their glycan and hydrophobic parts. These
analyses revealed that P.c.chabaudi AS synthesizes a set of GPI-
biosynthesis intermediates and two potential GPI-anchor precursors
exhibiting the following structures: ethanolamine-phosphate [(alpha1-
2)mannose]mann o s e ( alpha 1 - 2 ) m a n n o s e ( alpha 1 - 6 ) m a
n n o s e ( alpha 1 - 4 ) g l u c o s a m i n e - ( a c y l ) inositol-
phosphate-diacylglycerol (P.ch. alpha) and ethanolamine-p h o s p h a t
e - m a n n o s e ( alpha 1 - 2 ) m a n n o s e ( alpha 1 - 6 ) m a n n
o s e ( alpha 1 - 4 ) g l u c o samine-(acyl)inositol-phosphate-
diacylglycerol (P.ch. beta). One of these GPI-anchor precursors (P.ch.
alpha) possesses the same carbohydrate structure as the GPI membrane
anchor of merozoite surface protein-1 from P.c.chabaudi AS.
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