UI  - 0
AU  - Gerold P
AU  - Vivas L
AU  - Ogun SA
AU  - Azzouz N
AU  - Brown KN
AU  - Holder AA
AU  - Schwarz RT
TI  - Glycosylphosphatidylinositols of Plasmodium chabaudi chabaudi: a basis
      for the study of malarial glycolipid toxins in a rodent model
LA  - ENG
PT  - JOURNAL ARTICLE
DA  - 19971215
DP  - 1997 Dec 15
IS  - 0264-6021
TA  - Biochem J
PG  - 905-11
IP  - 3
VI  - 328
JC  - 9YO
AB  - Free and protein-bound glycosylphosphatidylinositols (GPIs) of the
      blood stages of the rodent malarial parasite Plasmodium chabaudi
      chabaudi AS were identified and characterized. TLC analysis of material
      extracted by organic solvents from metabolically labelled parasites
      revealed a distinct set of glycolipids. These glycolipids were
      identified as GPIs by specific chemical and enzymic treatments and by
      structural analysis of their glycan and hydrophobic parts. These
      analyses revealed that P.c.chabaudi AS synthesizes a set of GPI-
      biosynthesis intermediates and two potential GPI-anchor precursors
      exhibiting the following structures: ethanolamine-phosphate [(alpha1-
      2)mannose]mann o s e ( alpha 1 - 2 ) m a n n o s e ( alpha 1 - 6 ) m a
      n n o s e ( alpha 1 - 4 ) g l u c o s a m i n e - ( a c y l ) inositol-
      phosphate-diacylglycerol (P.ch. alpha) and ethanolamine-p h o s p h a t
      e - m a n n o s e ( alpha 1 - 2 ) m a n n o s e ( alpha 1 - 6 ) m a n n
      o s e ( alpha 1 - 4 ) g l u c o samine-(acyl)inositol-phosphate-
      diacylglycerol (P.ch. beta). One of these GPI-anchor precursors (P.ch.
      alpha) possesses the same carbohydrate structure as the GPI membrane
      anchor of merozoite surface protein-1 from P.c.chabaudi AS.
AD  - Zentrum für Hygiene und Med. Mikrobiologie, Philipps-
      Universität, Robert-Koch Str. 17, 35037 Marburg, Germany
PMID- 9396737
SO  - Biochem J 1997 Dec 15;328(3):905-11